16 research outputs found
Resonances in rotationally inelastic scattering of OH() with helium and neon
We present detailed calculations on resonances in rotationally and spin-orbit
inelastic scattering of OH (X\,^2\Pi, j=3/2, F_1, f) radicals with He and Ne
atoms. We calculate new \emph{ab initio} potential energy surfaces for OH-He,
and the cross sections derived from these surfaces compare favorably with the
recent crossed beam scattering experiment of Kirste \emph{et al.} [Phys. Rev. A
\textbf{82}, 042717 (2010)]. We identify both shape and Feshbach resonances in
the integral and differential state-to-state scattering cross sections, and we
discuss the prospects for experimentally observing scattering resonances using
Stark decelerated beams of OH radicals.Comment: 14 pages, 15 Figure
RNA silencing can explain chlorotic infection patterns on plant leaves
<p>Abstract</p> <p>Background</p> <p>RNA silencing has been implicated in virus symptom development in plants. One common infection symptom in plants is the formation of chlorotic tissue in leaves. Chlorotic and healthy tissue co-occur on a single leaf and form patterns. It has been shown that virus levels in chlorotic tissue are high, while they are low in healthy tissue. Additionally, the presence of siRNAs is confined to the chlorotic spots and the boundaries between healthy and infected tissue. These results strongly indicate that the interaction between virus growth and RNA silencing plays a role in the formation of infection patterns on leaves. However, how RNA silencing leads to the intricate patterns is not known.</p> <p>Results</p> <p>Here we elucidate the mechanisms leading to infection patterns and the conditions which lead to the various patterns observed. We present a modeling approach in which we combine intra- and inter-cellular dynamics of RNA silencing and viral growth. We observe that, due to the spread of viruses and the RNA silencing response, parts of the tissue become infected while other parts remain healthy. As is observed in experiments high virus levels coincide with high levels of siRNAs, and siRNAs are also present in the boundaries between infected and healthy tissue. We study how single- and double-stranded cleavage by Dicer and amplification by RNA-dependent RNA polymerase can affect the patterns formed.</p> <p>Conclusion</p> <p>This work shows that RNA silencing and virus growth within a cell, and the local spread of virions and siRNAs between cells can explain the heterogeneous spread of virus in leaf tissue, and therewith the observed infection patterns in plants.</p
The dynamics and efficacy of antiviral RNA silencing: A model study
<p>Abstract</p> <p>Background</p> <p>Mathematical modeling is important to provide insight in the complicated pathway of RNA silencing. RNA silencing is an RNA based mechanism that is widely used by eukaryotes to fight viruses, and to control gene expression.</p> <p>Results</p> <p>We here present the first mathematical model that combines viral growth with RNA silencing. The model involves a plus-strand RNA virus that replicates through a double-strand RNA intermediate. The model of the RNA silencing pathway consists of cleavage of viral RNA into siRNA by Dicer, target cleavage of viral RNA via the RISC complex, and a secondary response. We found that, depending on the strength of the silencing response, different viral growth patterns can occur. Silencing can decrease viral growth, cause oscillations, or clear the virus completely. Our model can explain various observed phenomena, even when they seem contradictory at first: the diverse responses to the removal of RNA dependent RNA polymerase; different viral growth curves; and the great diversity in observed siRNA ratios.</p> <p>Conclusion</p> <p>The model presented here is an important step in the understanding of the natural functioning of RNA silencing in viral infections.</p
Low-energy inelastic collisions of oh radicals with he atoms and d-2 molecules
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99092.pdf (publisher's version ) (Open Access
Control and imaging of O(1D2) precession
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84442.pdf (publisher's version ) (Closed access)3 p