Emotional expressiveness of 5–6 month-old infants born very premature versus full-term at initial exposure to weaning foods

Facial expressions of 5–6 month-old infants born preterm and at term were compared while tasting for the first time solid foods (two fruit and two vegetable purées) given by the mother. Videotapes of facial reactions to these foods were objectively coded during the first six successive spoons of each test food using Baby FACS and subjectively rated by naïve judges. Infant temperament was also assessed by the parents using the Infant Behaviour Questionnaire. Contrary to our expectations, infants born preterm expressed fewer negative emotions than infants born full-term. Naïve judges rated infants born preterm as displaying more liking than their full-term counterparts when tasting the novel foods. The analysis of facial expressions during the six spoonfuls of four successive meals (at 1-week intervals) suggested a familiarization effect with the frequency of negative expressions decreasing after tasting the second spoon, regardless of infant age, type of food and order of presentation. Finally, positive and negative dimensions of temperament reported by the parents were related with objective and subjective coding of affective reactions toward foods in infants born preterm or full-term. Our research indicates that premature infants are more accepting of novel foods than term infants and this could be used for supporting the development of healthy eating patterns in premature infants. Further research is needed to clarify whether reduced negativity by infants born prematurely to the exposure to novel solid foods reflects a reduction of an adaptive avoidant behaviour during the introduction of novel foods

Assessment of sea surface temperature changes in the Gulf of Cadiz during the last 30 ka: Implications for glacial changes in the regional hydrography

New dinocyst analyses were conducted on core MD99-2339 retrieved from the central Gulf of Cadiz. Dinocyst and foraminiferal assemblages from this core are combined with existing data off SW Portugal and NW Morocco to investigate past hydrological and primary productivity regimes in the subtropical NE Atlantic Ocean over the last 30 ka. Our results have revealed highest upwelling intensity during Heinrich Stadial 1 (HS 1) and the Younger Dryas and weaker upwelling cells during the Last Glacial Maximum (LGM) and HS 2, off the SW Iberian and NW Moroccan margins. Similar assemblages between the Gulf of Cadiz and the NW Moroccan margin, and distinct species off Portugal, were observed during the cold climatic extremes that punctuated the last 30 ka. This pattern has been linked to the occurrence of a hydrological structure between SW Iberia and Cadiz during the last glacial period, perhaps similar to the modern Azores Front. This front was probably responsible locally for heterotrophic dinocysts found in the Gulf of Cadiz during the last glacial period, even if this sector is not conductive to upwelling phenomena by Ekman transport. Regional reconstructions of paleo-sea-surface temperatures (SSTs) using dinocyst and foraminiferal transfer functions, as well as alkenones, are also discussed and depict coherent scenarios over the last 30 ka. Seasonal reconstructions Correspondence to: A. Penaud ([email protected].) of LGM SSTs obtained with this multi-proxy panel are discussed jointly with model outputs in order to contribute to ongoing efforts in model-data comparisonC2007-FCT/319/2006info:eu-repo/semantics/publishedVersio

Real-time imaging of the bacillithiol redox potential in the human pathogen Staphylococcus aureus using a genetically encoded bacilliredoxin-fused redox biosensor

Aims: Bacillithiol (BSH) is utilized as major thiol-redox buffer in the human pathogen Staphylococcus aureus. Under oxidative stress, BSH forms mixed disulfides with proteins, termed as S-bacillithiolation which can be reversed by bacilliredoxins (Brx). In eukaryotes, glutaredoxin-fused roGFP2 biosensors have been applied for dynamic live-imaging of the glutathione redox potential. Here, we have constructed a genetically encoded bacilliredoxin-fused redox biosensor (Brx-roGFP2) to monitor dynamic changes in the BSH redox potential in S. aureus. Results: The Brx-roGFP2 biosensor showed a specific and rapid response to low levels bacillithiol disulphide (BSSB) in vitro which required the active-site Cys of Brx. Dynamic live-imaging in two methicillin-resistant S. aureus (MRSA) USA300 and COL strains revealed fast and dynamic responses of the Brx-roGFP2 biosensor under hypochlorite and H2O2 stress and constitutive oxidation of the probe in different BSH-deficient mutants. Furthermore, we found that the Brx-roGFP2 expression level and the dynamic range is higher in S. aureus COL compared to the USA300 strain. In phagocytosis assays with THP-1 macrophages, the biosensor was 87 % oxidized in S. aureus COL. However, no changes in the BSH redox potential were measured after treatment with different antibiotics classes indicating that antibiotics do not cause oxidative stress in S. aureus. Conclusion and Innovation: This Brx-roGFP2 biosensor catalyzes specific equilibration between the BSH and roGFP2 redox couples and can be applied for dynamic live imaging of redox changes in S. aureus and other BSH-producing Firmicutes

Why a new volume on Non-Pollen Palynomorphs?

Here we introduce SP511, Applications of Non-Pollen Palynomorphs: from Palaeoenvironmental Reconstructions to Biostratigraphy. The study of Non-pollen palynomorphs (NPPs) has a long and rich history that is interwoven with that of pollen-based studies. NPPs are among the oldest fossils on record, and are instrumental in determining the origin and evolution of life, as well as studying origination and extinction events prior to the origin of pollen-producing angiosperms. This new volume on NPPs provides an up-to-date and seminal overview of the subject, linking deep-time and Quaternary study of the subject for the first time

Late Little Ice Age palaeoenvironmental records from the Anzali and Amirkola Lagoons (south Caspian Sea): Vegetation and sea level changes

This is a postprint version of the article. The official published article can be found from the link below - Copyright @ 2011 Elsevier Ltd.Two internationally important Ramsar lagoons on the south coast of the Caspian Sea (CS) have been studied by palynology on short sediment cores for palaeoenvironmental and palaeoclimatic investigations. The sites lie within a small area of very high precipitation in a region that is otherwise dry. Vegetation surveys and geomorphological investigations have been used to provide a background to a multidisciplinary interpretation of the two sequences covering the last four centuries. In the small lagoon of Amirkola, the dense alder forested wetland has been briefly disturbed by fire, followed by the expansion of rice paddies from AD1720 to 1800. On the contrary, the terrestrial vegetation reflecting the diversity of the Hyrcanian vegetation around the lagoon of Anzali remained fairly complacent over time. The dinocyst and non-pollen palynomorph assemblages, revealing changes that have occurred in water salinity and water levels, indicate a high stand during the late Little Ice Age (LIA), from AD < 1620 to 1800–1830. In Amirkola, the lagoon spit remained intact over time, whereas in Anzali it broke into barrier islands during the late LIA, which merged into a spit during the subsequent sea level drop. A high population density and infrastructure prevented renewed breaking up of the spit when sea level reached its maximum (AD1995). Similar to other sites in the region around the southern CS, these two lagoonal investigations indicate that the LIA had a higher sea level as a result of more rainfall in the drainage basin of the CS.The coring and the sedimentological analyses were funded by the Iranian National Institute for Oceanography in the framework of a research project entitled “Investigation of the Holocene sediment along the Iranian coast of Caspian Sea: central Guilan”. The radiocarbon date of core HCGL02 was funded by V. Andrieu (Europôle Méditerranéen de l'Arbois, France) and that of core HCGA04 by Brunel University

Arctic sea-ice proxies: Comparisons between biogeochemical and micropalaeontological reconstructions in a sediment archive from Arctic Canada

Boxcore 99LSSL-001 from the southwest Canadian Arctic Archipelago (68.095°N, 114.186°W), studied by multiproxy approaches (sea-ice diatom biomarker IP25, phytoplankton-based biomarker brassicasterol, biogenic silica, total organic carbon, dinoflagellate cysts = dinocysts, diatoms) and their applications (sea-ice index PBIP25, modern analogue technique (MAT) transfer functions), provides a chronologically constrained (210Pb, 137Cs, two 14C dates) palaeoenvironmental archive spanning AD 1625–1999 with which to compare and evaluate proxies frequently used in sea-ice reconstructions. Whereas diatoms are rare, PBIP25, biogenic silica and qualitative dinocyst approaches show good agreement, suggesting that palaeo sea-ice histories based on biomarker and microfossil techniques are robust in this region. These combined approaches show fluctuating long open water to marginal ice zone conditions (AD 1625–1740), followed by high-amplitude oscillations between long open water and extended spring/summer sea ice (AD 1740–1870). Greater ice cover (AD 1870–1970) precedes recent reductions in seasonal sea ice (AD 1970–1999). Dinocyst-based MAT, however, produces a low-amplitude signal lacking the nuances of other proxies, with most probable sea-ice reconstructions poorly correlating with biomarker-based histories. Explanations for this disagreement may include limited spatial coverage in the modern dinocyst distribution database for MAT and the broad environmental tolerances of polar dinocysts. Overall, PBIP25 provides the most detailed palaeo sea-ice signal, although its use in a shallow polar archipelago downcore setting poses methodological challenges. This proxy comparison demonstrates the limitations of palaeo sea-ice reconstructions and emphasizes the need for calibration studies tying modern microfossil and biogeochemical proxies to directly measured oceanographic parameters, as a springboard for robust quantitative palaeo studies. </jats:p

Terrorism discourse on French international broadcasting: <i>France 24</i> and the case of <i>Charlie Hebdo</i> attacks in Paris

This article offers an inquiry into the discursive construction of ‘terrorism’ by France 24, the French international broadcaster, in the aftermath of the attacks on Charlie Hebdo satirical magazine in January 2015. The article argues that the broadcaster seems to employ a relatively narrow definition of terrorism linking it to Islam and Muslims. France 24 portrays the attacks as an external phenomenon coming to France from outside. The blame is assigned to non-French factors, mainly to foreign extremist organisations, Islamist ideologues and overseas training. No reasons for violence are sought inside the country. Internal developments, such as discrimination, youth marginalisation, lack of educational and work opportunities, relations between law enforcement and the Muslim community that could potentially contribute to the acts, are not explored by the broadcaster’s investigative journalism. This narrow interpretation of ‘terrorism’ that assigns responsibility to Muslims, Islamic indoctrination and overseas training may further alienate Muslim communities in France’s already divided society. It points to narrow policy responses that focus mainly on stricter monitoring of Muslim minorities, on limiting combat and cross-border movement. This type of discourse excludes long-term policy solutions that address broader socio-politico-economic conditions in which ‘terrorism’ might flourish

Rapid tests and urine sampling techniques for the diagnosis of urinary tract infection (UTI) in children under five years: a systematic review

Background: Urinary tract infection (UTI) is one of the most common sources of infection in children under five. Prompt diagnosis and treatment is important to reduce the risk of renal scarring. Rapid, cost-effective, methods of UTI diagnosis are required as an alternative to culture. Methods: We conducted a systematic review to determine the diagnostic accuracy of rapid tests for detecting UTI in children under five years of age. Results: The evidence supports the use of dipstick positive for both leukocyte esterase and nitrite (pooled LR+ = 28.2, 95% CI: 17.3, 46.0) or microscopy positive for both pyuria and bacteriuria (pooled LR+ = 37.0, 95% CI: 11.0, 125.9) to rule in UTI. Similarly dipstick negative for both LE and nitrite (Pooled LR- = 0.20, 95% CI: 0.16, 0.26) or microscopy negative for both pyuria and bacteriuria (Pooled LR- = 0.11, 95% CI: 0.05, 0.23) can be used to rule out UTI. A test for glucose showed promise in potty-trained children. However, all studies were over 30 years old. Further evaluation of this test may be useful. Conclusion: Dipstick negative for both LE and nitrite or microscopic analysis negative for both pyuria and bacteriuria of a clean voided urine, bag, or nappy/pad specimen may reasonably be used to rule out UTI. These patients can then reasonably be excluded from further investigation, without the need for confirmatory culture. Similarly, combinations of positive tests could be used to rule in UTI, and trigger further investigation

Perioperative celecoxib administration for pain management after total knee arthroplasty – A randomized, controlled study

<p>Abstract</p> <p>Background</p> <p>Non-steroidal anti-inflammatory drugs (NSAIDs) are recommended for multimodal postoperative pain management. We evaluated opioid-sparing effects and rehabilitative results after perioperative celecoxib administration for total knee arthroplasty.</p> <p>Methods</p> <p>This was a prospective, randomized, observer-blind control study. Eighty patients that underwent total knee arthroplasty were randomized into two groups of 40 each. The study group received a single 400 mg dose of celecoxib, one hour before surgery, and 200 mg of celecoxib every 12 hours for five days, along with patient-controlled analgesic (PCA) morphine. The control group received only PCA morphine for postoperative pain management. Visual analog scale (VAS) pain scores, active range of motion (ROM), total opioid use and postoperative nausea/vomiting were analyzed.</p> <p>Results</p> <p>Groups were comparable for age, pre-operative ROM, operation duration and intraoperative blood loss. Resting VAS pain scores improved significantly in the celecoxib group, compared with controls, at 48 hrs (2.13 ± 1.68 vs. 3.43 ± 1.50, p = 0.03) and 72 hrs (1.78 ± 1.66 vs. 3.17 ± 2.01, p = 0.02) after surgery. Active ROM also increased significantly in the patients that received celecoxib, especially in the first 72 hrs [40.8° ± 17.3° vs. 25.8° ± 11.5°, p = 0.01 (day 1); 60.7° ± 18.1° vs. 45.0° ± 17.3°, p = 0.004 (day 2); 77.7° ± 15.1° vs. 64.3° ± 16.9°, p = 0.004 (day 3)]. Opioid requirements decreased about 40% (p = 0.03) in the celecoxib group. Although patients suffering from post-operative nausea/vomiting decreased from 43% in control group to 28% in celecoxib group, this was not significant (p = 0.57). There were no differences in blood loss (intra- and postoperative) between the groups. Celecoxib resulted in no significant increase in the need for blood transfusions.</p> <p>Conclusion</p> <p>Perioperative celecoxib significantly improved postoperative resting pain scores at 48 and 72 hrs, opioid consumption, and active ROM in the first three days after total knee arthroplasty, without increasing the risks of bleeding.</p> <p>Trial registration</p> <p>Clinicaltrials.gov NCT00598234</p