Vivre au chevet de la mort : une analyse phénoménologique et interprétative de l’expérience spirituelle et existentielle d’infirmières qui accompagnent des patients en fin de vie

La présente thèse poursuit l’objectif global de mieux comprendre comment, dans un contexte socioculturel où la mort est déniée, des soignants arrivent à se préserver psychiquement en faisant quotidiennement face à la réalité existentielle de la finitude. Pour ce faire, nous proposons une exploration détaillée de l’expérience spirituelle et existentielle d’infirmières qui accompagnent des patients en fin de vie. Cette thèse est présentée sous forme de trois articles. Le premier article vise, par une analyse conceptuelle systématique et exhaustive de la littérature empirique, à élaborer une définition intégrative du concept de spiritualité en contexte de fin de vie. La synthèse effectuée a permis de définir le concept de spiritualité comme étant « un processus de développement conscient qui se caractérise par deux mouvements de transcendance; celui d’aller profondément à l’intérieur de soi ou encore au-delà de soi-même. » Le deuxième article a pour but d’exposer une meilleure compréhension (a) de la façon dont les infirmières qui accompagnent des patients en fin de vie gèrent la confrontation quotidienne à la finitude, ainsi que (b) des dimensions spirituelles et existentielles de leur expérience. Des entrevues qualitatives en profondeur ont été menées auprès de 11 infirmières en soins palliatifs. L’analyse phénoménologique et interprétative des données recueillies a permis d’établir une typologie, laquelle fait état de trois modes de régulation psychique dans la confrontation à la finitude : « la mort intégrée », « la mort à combattre » et « la mort-souffrance ». De plus, les résultats de l’analyse suggèrent une relation potentielle entre les mécanismes de régulation psychique des infirmières, leur expérience spirituelle et existentielle personnelle, ainsi que leur mode de régulation intersubjective dans la relation avec les patients. Le troisième article vise pour sa part à décrire les effets d’une intervention d’approche existentielle de groupe sur l’expérience spirituelle et existentielle d’infirmières, tant au niveau personnel que professionnel. Pour ce faire, 10 infirmières ont été rencontrées en entrevues suite à leur participation à une intervention existentielle de groupe. Selon la perspective des infirmières, la participation à l’intervention existentielle leur a permis d’élargir leur conscience spirituelle et existentielle, notamment par une conscience accrue (a) de la finitude, (b) des sources de sens à la vie, (c) du sens de la souffrance, ainsi que (d) par la possibilité d’accéder à un état de gratitude. Les participants ont de plus bénéficié de la fonction « contenante » de l’expérience de groupe, en développant un langage partagé pour discuter des dimensions spirituelle et existentielle de leur expérience et ainsi qu’en éprouvant un sentiment de validation de leur expérience par le partage entre pairs. En conclusion, les résultats de notre analyse soulignent l’importance des différences individuelles dans les mécanismes de régulation psychique impliqués dans la confrontation à la mort chez les infirmières. De plus, le bilan des articles proposés permet de tisser des liens conceptuels cohérents pour supporter la thèse du rôle potentiellement catalyseur de la confrontation à la mort dans le développement de l’expérience spirituelle et existentielle des infirmières qui accompagnent des patients en fin de vieThe global objective of this thesis is to develop a better understanding of how health care providers psychologically negotiate death proximity, given the common contextual tendencies towards denial of death. To address this objective, we suggest an in-depth exploration of the spiritual and existential experience of palliative care nurses. This thesis is comprised of three articles. The first article is a systematic and exhaustive conceptual analysis of the empirical literature that provides an integrative definition of the concept of spirituality at the end of life. The resulting synthesis suggests that spirituality can be defined as “developmental and conscious process, characterized by two movements of transcendence; either deep within the self or beyond the self.” The objectives of the second article are to derive a better understanding of (a) how palliative care nurses handle daily confrontation with death, and (b) the spiritual and existential dimensions of their experience. In-depth qualitative interviews were conducted with 11 palliative care nurses. An Interpretative Phenomenological Analysis of the data allowed the emergence of a typology that described three modes of affective regulation of death confrontation: “Integrating death”, “fighting death”, and “suffering death”. Furthermore, the results suggest a potential relationship between these three factors: (a) nurses’ affective regulation, (b) spiritual-existential experiences' and (3) intersubjective regulation in relationship with patients. The third article describes the effects of a Meaning-Centered Intervention on the spiritual and existential dimensions of nurses’ experience, both at a personal and at a professional level. Ten nurses were interviewed individually after participating in a group Meaning-Centered Intervention. From the nurses’ point of view, the intervention helped them to enlarge their spiritual and existential awareness by encouraging them to become more conscious of (a) the finiteness of life, (2) the sources of meaning in life, (3) the meaning of suffering and (4) the impact of mindfulness. Moreover, participants reported to have benefited from the containing function of the group experience by developing a common language and feeling validated through sharing their experience with peers. In conclusion, our results indicate the importance of individual differences in the psychological mechanisms involved in nurses’ experience of confrontation with death. Taken as a whole, the findings of the three articles suggest a coherent conceptual link between the role of death confrontation in the development of spiritual and existential experiences for palliative care nurses

“Growing from an Invisible Wound” A Humanistic-Existential Approach to PTSD

From a humanistic and existential perspective, posttraumatic stress disorder (PTSD) can be understood as a normal response to a threatening existential event. The humanistic-existential approach to understanding and treating PTSD also places particular emphasis on the meaning of the traumatic experience and on the awareness of the existential part of the self. Such an understanding conveys to a different approach to trauma assessment and potential for healing in the clinical encounter. In this chapter, we wish to provide a humanistic-existential understanding of trauma. To do so, we review the key humanistic-existential concepts for trauma conceptualization, assessment, and intervention. Afterwards, we present two different short case studies to illustrate and understand the humanistic-existential psychotherapeutic process and its diversity. In conclusion, we discuss the contribution and limits of a humanistic-existential approach to trauma conceptualization, assessment, and healing

Kinetics of pore formation by the Bacillus thuringiensis toxin Cry1Ac

AbstractAfter binding to specific receptors, Cry toxins form pores in the midgut apical membrane of susceptible insects. The receptors could form part of the pore structure or simply catalyze pore formation and consequently be recycled. To discriminate between these possibilities, the kinetics of pore formation in brush border membrane vesicles isolated from Manduca sexta was studied with an osmotic swelling assay. Pore formation, as deduced from changes in membrane permeability induced by Cry1Ac during a 60-min incubation period, was strongly dose-dependent, but rapidly reached a maximum as toxin concentration was increased. Following exposure of the vesicles to the toxin, the osmotic swelling rate reached a maximum shortly after a delay period. Under these conditions, at relatively high toxin concentrations, the maximal osmotic swelling rate increased linearly with toxin concentration. When vesicles were incubated for a short time with the toxin and then rapidly cooled to prevent the formation of new pores before and during the osmotic swelling experiment, a plateau in the rate of pore formation was observed as toxin concentration was increased. Taken together, these results suggest that the receptors do not act as simple catalysts of pore formation, but remain associated with the pores once they are formed

Functional mechanisms underlying pleiotropic risk alleles at the 19p13.1 breast-ovarian cancer susceptibility locus

A locus at 19p13 is associated with breast cancer (BC) and ovarian cancer (OC) risk. Here we analyse 438 SNPs in this region in 46,451 BC and 15,438 OC cases, 15,252 BRCA1 mutation carriers and 73,444 controls and identify 13 candidate causal SNPs associated with serous OC (P=9.2 × 10-20), ER-negative BC (P=1.1 × 10-13), BRCA1-associated BC (P=7.7 × 10-16) and triple negative BC (P-diff=2 × 10-5). Genotype-gene expression associations are identified for candidate target genes ANKLE1 (P=2 × 10-3) and ABHD8 (P<2 × 10-3). Chromosome conformation capture identifies interactions between four candidate SNPs and ABHD8, and luciferase assays indicate six risk alleles increased transactivation of the ADHD8 promoter. Targeted deletion of a region containing risk SNP rs56069439 in a putative enhancer induces ANKLE1 downregulation; and mRNA stability assays indicate functional effects for an ANKLE1 3′-UTR SNP. Altogether, these data suggest that multiple SNPs at 19p13 regulate ABHD8 and perhaps ANKLE1 expression, and indicate common mechanisms underlying breast and ovarian cancer risk

The FANCM:p.Arg658* truncating variant is associated with risk of triple-negative breast cancer

Abstract: Breast cancer is a common disease partially caused by genetic risk factors. Germline pathogenic variants in DNA repair genes BRCA1, BRCA2, PALB2, ATM, and CHEK2 are associated with breast cancer risk. FANCM, which encodes for a DNA translocase, has been proposed as a breast cancer predisposition gene, with greater effects for the ER-negative and triple-negative breast cancer (TNBC) subtypes. We tested the three recurrent protein-truncating variants FANCM:p.Arg658*, p.Gln1701*, and p.Arg1931* for association with breast cancer risk in 67,112 cases, 53,766 controls, and 26,662 carriers of pathogenic variants of BRCA1 or BRCA2. These three variants were also studied functionally by measuring survival and chromosome fragility in FANCM−/− patient-derived immortalized fibroblasts treated with diepoxybutane or olaparib. We observed that FANCM:p.Arg658* was associated with increased risk of ER-negative disease and TNBC (OR = 2.44, P = 0.034 and OR = 3.79; P = 0.009, respectively). In a country-restricted analysis, we confirmed the associations detected for FANCM:p.Arg658* and found that also FANCM:p.Arg1931* was associated with ER-negative breast cancer risk (OR = 1.96; P = 0.006). The functional results indicated that all three variants were deleterious affecting cell survival and chromosome stability with FANCM:p.Arg658* causing more severe phenotypes. In conclusion, we confirmed that the two rare FANCM deleterious variants p.Arg658* and p.Arg1931* are risk factors for ER-negative and TNBC subtypes. Overall our data suggest that the effect of truncating variants on breast cancer risk may depend on their position in the gene. Cell sensitivity to olaparib exposure, identifies a possible therapeutic option to treat FANCM-associated tumors

Functional mechanisms underlying pleiotropic risk alleles at the 19p13.1 breast-ovarian cancer susceptibility locus

A locus at 19p13 is associated with breast cancer (BC) and ovarian cancer (OC) risk. Here we analyse 438 SNPs in this region in 46,451 BC and 15,438 OC cases, 15,252 BRCA1 mutation carriers and 73,444 controls and identify 13 candidate causal SNPs associated with serous OC (P = 9.2 x 10(-20)), ER-negative BC (P = 1.1 x 10(-13)), BRCA1-associated BC (P = 7.7 x 10(-16)) and triple negative BC (P-diff = 2 x 10(-5)). Genotype-gene expression associations are identified for candidate target genes ANKLE1 (P = 2 x 10(-3)) and ABHD8 (PPeer reviewe

A case-only study to identify genetic modifiers of breast cancer risk for BRCA1/BRCA2 mutation carriers

Breast cancer (BC) risk for BRCA1 and BRCA2 mutation carriers varies by genetic and familial factors. About 50 common variants have been shown to modify BC risk for mutation carriers. All but three, were identified in general population studies. Other mutation carrier-specific susceptibility variants may exist but studies of mutation carriers have so far been underpowered. We conduct a novel case-only genome-wide association study comparing genotype frequencies between 60,212 general population BC cases and 13,007 cases with BRCA1 or BRCA2 mutations. We identify robust novel associations for 2 variants with BC for BRCA1 and 3 for BRCA2 mutation carriers, P < 10−8, at 5 loci, which are not associated with risk in the general population. They include rs60882887 at 11p11.2 where MADD, SP11 and EIF1, genes previously implicated in BC biology, are predicted as potential targets. These findings will contribute towards customising BC polygenic risk scores for BRCA1 and BRCA2 mutation carriers

Le groupe de discussion comme espace de soutien : briser l’isolement chez des survivantes du génocide rwandais

En plus d’être une technique riche et utile de cueillette de données qualitatives, le dispositif du groupe de discussion peut remplir d’autres fonctions méthodologiques, voire cliniques, comme nous le proposons dans cet article. En soutenant nos propos par des données issues d’un travail ethnographique, dont l’objectif était de tenter de mieux comprendre l’expérience de survie et d’adaptation de femmes rescapées du génocide rwandais, nous suggérons que l’utilisation du groupe de discussion, dans une optique de vérification des résultats auprès des participantes, peut aussi permettre de soutenir le critère de rigueur d’authenticité d’une démarche naturaliste-constructiviste (Morrow, 2005). En outre, nous explorons en quoi le recours au groupe de discussion peut être un espace thérapeutique pour ses participantes, tel qu’il fut le cas dans le contexte de notre ethnographie au Rwanda

Ethnographie rwandaise sur l’apport subjectif bénéfique de l’interprète dans l’analyse de données

Dans le cadre de ce numéro spécial sur la recherche qualitative en contexte africain, nous suggérons que, bien que communément considéré comme une source de biais, le travail conjoint avec un interprète en contexte ethnographique peut s’avérer une source précieuse de données à utiliser dans la compréhension du phénomène à l’étude. En retraçant l’évolution de la démarche réflexive inhérente à une cueillette de données ethnographiques sur l’expérience de survie et d’adaptation de femmes rescapées du génocide rwandais, nous proposons que la relation établie avec notre interprète nous a permis de saisir l’essence de l’expérience traumatique des survivantes