Influence of Metal-Coordinating Comonomers on the Coordination Structure and Binding in Magnetic Poly(ionic Liquid)s

A poly(ionic liquid) (PIL), poly(acrylamide-co-diallyl dimethylammonium chloride), was systematically complexed with Co2+, Fe3+, and Co2+/Fe3+ (mixed) at different molar equivalencies to form a series of magnetic PILs (MPILs). These novel MPILs were utilized to examine molecular structure and binding between the polymer and metal species using comprehensive spectroscopic studies in both dry and liquid states. FTIR, X-ray photoelectron, UV-vis, and Raman spectroscopies showed evidence of metal coordination of both the iron and cobalt chloride species with the acrylamide comonomer. Using AC susceptibility measurements, the MPILs were found to have magnetic properties within the range of typical MPIL homopolymers with magnetic mass susceptibilities dependent on metal cation type and concentration. This work demonstrates MPILs can be tuned using the metal ion species and concentration, to increase the magnetic mass susceptibility and alter metal-ion coordination structure and binding with the PIL copolymer. Furthermore, the coordination structure of the transition metal halide complex was determined to be dependent on metal halide concentration in the polymer for the iron-based system, and it was shown that both metal cations (Fe3+ and Co2+) bond not only electrostatically with the PIL monomer but also predominantly with the acrylamide comonomer. In particular, metal–oxygen and metal–nitrogen bonding was observed with the amide group present in the acrylamide. Understanding and controlling the coordination structure and binding in PILs is significant as these materials allow for the formability and flexibility advantages of polymers to be combined with properties atypical for polymers, such as high ion conductivity, electrical and thermal stability, antibacterial properties, and magnetic responsiveness, in the case of MPILs

A systematic literature review of undergraduate clinical placements in underserved areas.

Context: The delivery of undergraduate clinical education in underserved areas is increasing in various contexts across the world in response to local workforce needs. A collective understanding of the impact of these placements is lacking. Previous reviews have often taken a positivist approach by only looking at outcome measures. This review addresses the question: What are the strengths and weaknesses for medical students and supervisors of community placements in underserved areas? Methods: A systematic literature review was carried out by database searching, citation searching, pearl growing, reference list checking and use of own literature. The databases included MEDLINE, EMBASE, PsycINFO, Web of Science and ERIC. The search terms used were combinations and variations of four key concepts exploring general practitioner (GP) primary care, medical students, placements and location characteristics. The papers were analysed using a textual narrative synthesis. Findings: The initial search identified 4923 results. After the removal of duplicates and the screening of titles and abstracts, 185 met the inclusion criteria. These full articles were obtained and assessed for their relevance to the research question; 54 were then included in the final review. Four main categories were identified: student performance, student perceptions, career pathways and supervisor experiences. Conclusions: This review reflects the emergent qualitative data as well as the quantitative data used to assess initiatives. Underserved area placements have produced many beneficial implications for students, supervisors and the community. There is a growing amount of evidence regarding rural, underserved areas, but little in relation to inner city, deprived areas, and none in the UK

Christian ethics and political economy

The value-free and relativistic human and scientific discourses have led to an era of ideology. From fascism at the dawn of the century, through liberalism and the associated phenomenon of unfettered statism, to the current disillusionment of postmodernism and relativism with endeavours towards new mercantilism. All have maintained poverty, inequality and created scepticism amongst both lay persons and academics. Above all else a renewed yearning for moral and ethical direction in political and economic conduct has been created. This book provides a Christian ethical reflection on political-economic conduct in South Africa as an alternative to current modernistic ideas. This book aims to produce new Christian ethical insight into the value of new liberal perspectives on the enhancement of the South African political economy. New Christian ethical insight will be gained through new perspectives on the South African political economy

Visual adaptation to goal-directed hand actions

Prolonged exposure to visual stimuli, or adaptation, often results in an adaptation “aftereffect” which can profoundly distort our perception of subsequent visual stimuli. This technique has been commonly used to investigate mechanisms underlying our perception of simple visual stimuli, and more recently, of static faces. We tested whether humans would adapt to movies of hands grasping and placing different weight objects. After adapting to hands grasping light or heavy objects, subsequently perceived objects appeared relatively heavier, or lighter, respectively. The aftereffects increased logarithmically with adaptation action repetition and decayed logarithmically with time. Adaptation aftereffects also indicated that perception of actions relies predominantly on view-dependent mechanisms. Adapting to one action significantly influenced the perception of the opposite action. These aftereffects can only be explained by adaptation of mechanisms that take into account the presence/absence of the object in the hand. We tested if evidence on action processing mechanisms obtained using visual adaptation techniques confirms underlying neural processing. We recorded monkey superior temporal sulcus (STS) single-cell responses to hand actions. Cells sensitive to grasping or placing typically responded well to the opposite action; cells also responded during different phases of the actions. Cell responses were sensitive to the view of the action and were dependent upon the presence of the object in the scene. We show here that action processing mechanisms established using visual adaptation parallel the neural mechanisms revealed during recording from monkey STS. Visual adaptation techniques can thus be usefully employed to investigate brain mechanisms underlying action perception.Publisher PDFPeer reviewe

Intravital FRAP imaging using an E-cadherin-GFP mouse reveals disease- and drug-dependent dynamic regulation of cell-cell junctions in live tissue

E-cadherin-mediated cell-cell junctions play a prominent role in maintaining the epithelial architecture. The disruption or deregulation of these adhesions in cancer can lead to the collapse of tumor epithelia that precedes invasion and subsequent metastasis. Here we generated an E-cadherin-GFP mouse that enables intravital photobleaching and quantification of E-cadherin mobility in live tissue without affecting normal biology. We demonstrate the broad applications of this mouse by examining E-cadherin regulation in multiple tissues, including mammary, brain, liver, and kidney tissue, while specifically monitoring E-cadherin mobility during disease progression in the pancreas. We assess E-cadherin stability in native pancreatic tissue upon genetic manipulation involving Kras and p53 or in response to anti-invasive drug treatment and gain insights into the dynamic remodeling of E-cadherin during in situ cancer progression. FRAP in the E-cadherin-GFP mouse, therefore, promises to be a valuable tool to fundamentally expand our understanding of E-cadherin-mediated events in native microenvironments

Higher Activity of the Inducible Nitric Oxide Synthase Contributes to Very Early Onset Inflammatory Bowel Disease

OBJECTIVES: The NOS2 gene encodes for the inducible nitric oxide synthase (iNOS), responsible for nitric oxide (NO) production, which contributes to antimicrobial and antipathogenic activities. Higher levels of both iNOS and NO-induced damage have been observed in inflammatory bowel disease (IBD) patients. NOS2 may have a role in a specific subset of IBD patients with severe and/or extensive colitis. Therefore, the aim of this study is to examine the role of NOS2 in such a subset, very early onset IBD (VEO-IBD). METHODS: Seventeen tag single nucleotide polymorphisms (SNPs) in the NOS2 gene were successfully genotyped in VEO-IBD patients. Genetic associations were replicated in an independent VEO-IBD cohort. Functional analysis for iNOS activity was performed on the most significantly associated functional variant. RESULTS: The NOS2 rs2297518 SNP was found to be associated in VEO-IBD in two independent cohorts. Upon combined analysis, a coding variant (S608L) showed the strongest association with VEO-IBD (Pcombined=1.13 × 10−6, OR (odds ratio)=3.398 (95% CI (confidence interval) 2.02–5.717)) as well as associations with VEO-Crohn's disease and VEO-ulcerative colitis (UC). This variant also showed an association with UC diagnosed between 11 and 17 years of age but not with adult-onset IBD (>17 years). B-cell lymphoblastoid cell lines genotyped for the risk variant as well as Henle-407 cells transfected with a plasmid construct with the risk variant showed higher NO production. Colonic biopsies of VEO-IBD patients showed higher immunohistochemical staining of nitrotyrosine, indicating more nitrosative stress and tissue damage. CONCLUSIONS: These studies suggest the importance of iNOS in genetic susceptibility to younger IBD presentation due to higher NO production

Association between ants (Hymenoptera: Formicidae) and the vine mealybug (Hemiptera: Pseudococcidae) in table-grape vineyards in Eastern Spain

[EN] BACKGROUNDThe vine mealybug, Planococcus ficus (Signoret) (Hemiptera: Pseudococcidae) is a key pest of grapevine in the Mediterranean Basin. Some honeydew collecting ant species are known to increase mealybug populations in other grape-growing regions. However, there is scarce information on either the ant species present in Mediterranean vineyards or their impact on mealybugs. We conducted a study in four commercial vineyards in Eastern Spain in order to i) identify the ant species foraging on the vine canopies, ii) study the association among ant activity, vine mealybug abundance and fruit damage, and iii) test a novel method for ant management, distracting ants from guarding vine mealybugs by providing sugar dispensers. RESULTSWe recorded three ant species native to the Mediterranean foraging on the vine canopies: Lasius grandis (Forel), Pheidole pallidula (Nylander) and Plagiolepis schmitzii (Forel). The mean percentage of damaged fruits per vine was positively correlated with the number of vine mealybugs captured in traps placed at the trunk. We detected a positive but weak relationship between ant activity, vine mealybug abundance and fruit damage. The provisioning of sugar dispensers reduced the number of ants foraging on the vines by 23.4% although this reduction was not statistically significant. Vine mealybug abundance was significantly reduced (72%) after sugar provisioning. CONCLUSIONOur results suggest that the ant species native to vineyards in eastern Spain induce population increases of the vine mealybug. Moreover, the provisioning of sugars can be a valuable tool for ant management and mealybug control. (c) 2017 Society of Chemical IndustryWe are grateful to two anonymous reviewers for their comments. This research was supported by the European grants FP7-IAPP #324475 'Colbics' and FP7-IRSES #612566 'Biomodics'.Beltrà Ivars, A.; Navarro Campos, C.; Calabuig Gomar, A.; Estopa, L.; Wäckers, F.; Pekas, A.; Soto Sánchez, AI. (2017). Association between ants (Hymenoptera: Formicidae) and the vine mealybug (Hemiptera: Pseudococcidae) in table-grape vineyards in Eastern Spain. Pest Management Science. 73(12):2473-2480. https://doi.org/10.1002/ps.4640S247324807312Ramzi, M., Kaouthar, G.-L., Pompeo, S., Gaetana, M., & Agatino, R. (2017). Key scale insects (Hemiptera: Coccoidea) of high economic importance in a Mediterranean area: host plants, bio-ecological characteristics, natural enemies and pest management strategies – a review. Plant Protection Science, 53(No. 1), 1-14. doi:10.17221/53/2016-ppsDaane, K. M., Almeida, R. P. P., Bell, V. A., Walker, J. T. S., Botton, M., Fallahzadeh, M., … Zaviezo, T. (2012). Biology and Management of Mealybugs in Vineyards. 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More Aggressive Ant Species (Hymenoptera: Formicidae) Provide Better Protection for Soft Scales and Mealybugs (Homoptera: Coccidae, Pseudococcidae). Biotropica, 23(3), 282. doi:10.2307/2388205Tena, A., Hoddle, C. D., & Hoddle, M. S. (2013). Competition between honeydew producers in an ant–hemipteran interaction may enhance biological control of an invasive pest. Bulletin of Entomological Research, 103(6), 714-723. doi:10.1017/s000748531300045xMgocheki, N., & Addison, P. (2010). Spatial distribution of ants (Hymenoptera: Formicidae), vine mealybugs and mealybug parasitoids in vineyards. Journal of Applied Entomology, 134(4), 285-295. doi:10.1111/j.1439-0418.2009.01494.xMgocheki, N., & Addison, P. (2009). Interference of ants (Hymenoptera: Formicidae) with biological control of the vine mealybug Planococcus ficus (Signoret) (Hemiptera: Pseudococcidae). 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Intravital FRAP Imaging using an E-cadherin-GFP Mouse Reveals Disease- and Drug-Dependent Dynamic Regulation of Cell-Cell Junctions in Live Tissue.

E-cadherin-mediated cell-cell junctions play a prominent role in maintaining the epithelial architecture. The disruption or deregulation of these adhesions in cancer can lead to the collapse of tumor epithelia that precedes invasion and subsequent metastasis. Here we generated an E-cadherin-GFP mouse that enables intravital photobleaching and quantification of E-cadherin mobility in live tissue without affecting normal biology. We demonstrate the broad applications of this mouse by examining E-cadherin regulation in multiple tissues, including mammary, brain, liver, and kidney tissue, while specifically monitoring E-cadherin mobility during disease progression in the pancreas. We assess E-cadherin stability in native pancreatic tissue upon genetic manipulation involving Kras and p53 or in response to anti-invasive drug treatment and gain insights into the dynamic remodeling of E-cadherin during in situ cancer progression. FRAP in the E-cadherin-GFP mouse, therefore, promises to be a valuable tool to fundamentally expand our understanding of E-cadherin-mediated events in native microenvironments

Effects of antiplatelet therapy on stroke risk by brain imaging features of intracerebral haemorrhage and cerebral small vessel diseases: subgroup analyses of the RESTART randomised, open-label trial

Background Findings from the RESTART trial suggest that starting antiplatelet therapy might reduce the risk of recurrent symptomatic intracerebral haemorrhage compared with avoiding antiplatelet therapy. Brain imaging features of intracerebral haemorrhage and cerebral small vessel diseases (such as cerebral microbleeds) are associated with greater risks of recurrent intracerebral haemorrhage. We did subgroup analyses of the RESTART trial to explore whether these brain imaging features modify the effects of antiplatelet therapy