Fyn-Mediated Regulation of Protein Kinase A

Protein kinases are enzymes important for signal transduction in the regulation of cellular processes. The cAMP-dependent protein kinase A (PKA) has been previously reported to regulate the activity of the Src family kinase Fyn, an event important for cellular migration. This study aimed to characterize the reciprocal interaction, in which Fyn regulates PKA. In addition to our preliminary, unpublished findings that Fyn phosphorylates the PKA catalytic subunit at Y69 to increase its catalytic activity, we have shown through co-immunoprecipitation that Fyn physically associates with PKA in HEK293 cells. Quantitative mass spectrometry and subsequent biochemical validation shows that PKACα undergoes enhanced binding to a complex of centrosomal and Golgi-localized A-kinase anchoring proteins when Fyn is overexpressed, independent of Fyn kinase activity. Fyn was found in this complex, as well, implicating its involvement as an adaptor protein. Co-immunoprecipitation experiments with various Fyn alleles demonstrated the dispensability of the Fyn SH3 domain and the functioning SH2 domain in binding to PKACα. GST-fusion proteins containing either of these domains were also unable to enrich PKACα from HEK293 lysates. Fyn was found to bind PKACα independent of its association with the regulatory subunit, and preliminary data suggests that this interaction is direct through purified protein pulldown experiments. We hypothesize that this regulatory interaction activates PKA at the centrosome and Golgi apparatus, facilitating the potential phosphorylation of proximal substrates involved in cytoskeletal organization and mitotic processes

Margie Barritt to Mr. Meredith (5 October 1962)

https://egrove.olemiss.edu/mercorr_pro/1085/thumbnail.jp

The Effects of Self-Compassion and Shame on the Relationship Between Perfectionism and Depression

The following study examined how self-compassion and shame effect the relationship between adaptive/maladaptive perfectionism and depression. Hierarchical multiple regression was used to examine the predictive role of adaptive/maladaptive perfectionism, shame, and self-compassion on depression. This study included a sample size of 226 undergraduate and graduate students from a university in the Rocky Mountain region. Results from the multiple regression analysis found maladaptive perfectionism was a significant predictor of depression (β = .540, p \u3c .01), which supported findings from previous research. When shame and self-compassion were included, results indicated self-compassion (β = -.257, p \u3c .01) and shame (β = .382, p \u3c .01) were full mediators in the relationship between maladaptive perfectionism (β = .035, p = .707) and depression. The change in significance for maladaptive perfectionism from β = .540 to β = .035 was statistically significant (p \u3c .01) showing important mediating effects of self-compassion and shame. Interestingly, adaptive perfectionism was found to act as a suppressor variable in this study; which provided important theoretical and methodological implications for future research. Overall, results emphasized the importance of targeting decreasing shame and increasing self-compassion for those with depression and maladaptive perfectionistic behaviors and beliefs

Ovule development in seeded and seedless grapes

Female gametophyte and early embryo and endosperm development were examined in 3 seedless grape varieties, Himrod, Interlaken Seedless and N. Y. 15302, and in their seeded parent Ontario. A high proportion of functional embryo sacs was found at full bloom in all varieties.Fertilization occurred in all varieties.Precocious initiation of endosperm was observed with Himrod and extremely rapid development of endosperm was found with N. Y. 15302.Early degeneration of zygotes, failure of the zygotes to divide, and endosperm degeneration were observed in the seedless varieties with smallest seed remnants, Himrod and Interlaken Seedless.Imperfect development of the zygote and endosperm resulted in seed abortion (stenospermy) and seedless fruit (stenospermocarpy) in Himrod and Interlaken Seedless varieties

The rhetoric of disability : a Foucauldian discourse analysis.

The institution of disability, as it is perceived in the United States and most of Western civilization, signifies a site of oppression that has been historically neglected if not actively rejected by the hegemonic population of nondisabled people. While disability studies has congregated as a concerted effort of academic inquiry, scholars have largely approached disability from cultural and sociological perspectives and have focused on physical rather than cognitive disabilities. The field of rhetoric, as the study of discourse and its relation to the production and reception of meaning, offers vital perspectives for better understanding the institution of disability and may serve as a framework for first illuminating and then disempowering the discursive structures that have heretofore suggested that people with disabilities are deviant and inferior. This exposition aims to trace how and in what contexts the concept of disability has been formed as a rhetorical object via Foucauldian discourse analysis. The findings of this research elucidate how the disabled person has come to be known as a subject of the domain of disability through discursive knowledge-power relations. Though a rhetorical analysis may not alleviate the physical, intellectual, or emotional obstacles that may occur from the phenomena of disability, this project represents one step toward reshaping the oppressive paradigm of cultural thought regarding disability and the unjust hierarchization of human ability and worth

The controls of radioelement distribution in the Etive and Cairngorm granites: Implications for heat production

Radiometric, whole rock trace element and petrological studies are reported for two late Caledonian granite complexes from the Grampian Highlands, Scotland. These studies throw light on the magmatic history of the intrusions and. more particularly. on their radioelement geochemistry and heat production which is interpreted in a geothermal context. The Etive Complex is a multiphase intrusion, ranging from diorite to granite in composition, emplaced by a cauldron subsidence mechanism. Its complex magmatic history involved crystal fractionation, both in-situ and at depth, coupled with episodic magma mixing in a deep magma chamber. Radioelement contents (means for the whole complex; 12.7 ppm Th, 2.9 ppm U, 4.1% K20) increase with magmatic differentiation and are concentrically zoned in the N Cruachan and Starav units. Mass balance calculations, incorporating radiometric, whole rock trace element, fission track and accessory phase microprobe data, show that uranium and thorium contents were, initially, controlled by the crystallisation of apatite + zircon + sphene ± allanite and chevkinite. Later, thorite and monazite became important thorium-hosts. Locally, enhanced uranium levels in the Starav Granites followed expulsion and limited outward migration of uranium-rich residual fluids. The distribution of radioelements in surface samples suggests that heat production decreases with depth in some units. Similar studies have identified four units in the Cairngorm Granite; NE Granite - Porphyritic Granite - Microgranite - Main Granite. Radioelement contents increase with magmatic evolution from the N Granite to the Main Granite; 26.5 > 32.3 ppm Th, 4.3 > 10.1 ppm U, 4.6 > 4.7% K20 (mean values). Uranium and thorium contents were controlled, predominantly, by the crystallisation of apatite + zircon + sphene ± allanite in the NE Granite and of apatite + zircon + monazite + xenotime + Nb-Ta-oxides ± thorite ± uraninite in the Main Granite. Minor amounts of uranium reside in secondary sites in hydrothermally altered samples. Modelled surface heat flow anomalies are 5.8 mW m-2 and 23.0 mW m-2 for the Etive Complex and Cairngorm Granite respectively. Comparison of calculated and preliminary heat flow measurements in the Cairngorm granite indicates that at least 35% of the observed heat flow arises from radioactive sources in the granite and that background heat flow is low

Dr. Clay F. Barritt to James Meredith (1 October 1962)

https://egrove.olemiss.edu/mercorr_pro/1828/thumbnail.jp

Evidence that TRPM8 is an androgen-dependent Ca2+ channel required for the survival of prostate cancer cells

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