Exploring the evidence base for national and regional policy interventions to combat resistance

The effectiveness of existing policies to control antimicrobial resistance is not yet fully understood. A strengthened evidence base is needed to inform effective policy interventions across countries with different income levels and the human health and animal sectors. We examine three policy domains—responsible use, surveillance, and infection prevention and control—and consider which will be the most effective at national and regional levels. Many complexities exist in the implementation of such policies across sectors and in varying political and regulatory environments. Therefore, we make recommendations for policy action, calling for comprehensive policy assessments, using standardised frameworks, of cost-effectiveness and generalisability. Such assessments are especially important in low-income and middle-income countries, and in the animal and environmental sectors. We also advocate a One Health approach that will enable the development of sensitive policies, accommodating the needs of each sector involved, and addressing concerns of specific countries and regions

Effects of different treatment subjects of Lactiplantibacillus plantarum SCS5 on oxidative stress in type 2 diabetic mice

ABSTRACTThe purpose of this study was to determine the effect of different subjects from Lactiplantibacillus plantarum SCS5 (L. plantarum SCS5) on type 2 diabetes by modulating oxidative stress levels.The normal group (NG) and the model group (MG) of mice were fed normally, while the three test groups (LP1,LP2,LP3) received 10 ml/kg (body weight) of the L. plantarum SCS5 suspension, intracellular material, or heat-killed intracellular material daily for 10 weeks. The weight loss and the increase in blood glucose were alleviated in the LP2 and LP3 groups. Insulin, catalase, superoxide dismutase, and glutathione peroxidase significantly increased in the LP3 group of mice compared with the MG group. Additionally, the pathological sections showed that the lesions in the LP3 group were less severe than those in the MG group. These findings indicate that the heat-killed intracellular L. plantarum SCS5 material was most effective in alleviating oxidative stress in type 2 diabetic mice

3,3′-Diindolylmethane Enhances Fluorouracil Sensitivity via Inhibition of Pyrimidine Metabolism in Colorectal Cancer

Chemoresistance limits treatment outcomes in colorectal cancer (CRC) patients. A dimeric metabolite of indole-3-carbinol, 3,3′-diindolylmethane (DIM) is abundant in cruciferous vegetables and has shown anticancer efficacy. The role of DIM in regulating chemosensitivity in CRC remains unknown. In this study, we demonstrated that DIM treatment inhibits the malignant progression of CRC. RNA sequencing indicated that pyrimidine synthesis genes are attenuated by DIM treatment. Stable 13C-labeled glucose tracing revealed that DIM inhibits de novo pyrimidine biosynthesis in CRC. DIM increases 5-FU cytotoxicity in CRC via regulation of the expression of pyrimidine metabolism-related genes. DIM synergizes with 5-FU to enhance its inhibitory effects on CRC both in vivo and in vitro. Our results suggest that DIM improves the therapeutic outcomes of FU-based chemotherapy in CRCs by inhibiting pyrimidine metabolism, identifying a new strategy for clinical therapy

Monetary diet cost is positively associated with diet quality and obesity: an analysis of school-aged children in Southwest China

Background: Little is known about the relationships between diet cost, dietary intake and obesity in Chinese populations. This study explored how diet cost was related to diet quality and obesity among school-aged children in Southwest China. Methods: Data from a cross-sectional study was analysed. Diet cost was estimated based on dietary intake assessed with 24-h dietary recalls and retail food prices. Diet quality was measured using the Chinese Children Dietary Index. Body height, weight, waist circumference and skinfold thicknesses were measured, and their body mass index standard deviation score (BMISDS), waist-to-height ratio (WHtR), fat mass index (FMI) and fat-free mass index (FFMI) were calculated. Multivariate regression models were used to explore the relevance of diet cost to diet quality and obesity. Results: After adjustment for potential confounders, a positive association was observed between diet quality and energy-adjusted diet cost (β = 0.143, 95% confidence interval, CI: 0.014–0.285, Pfor-trend = 0.0006). Energy-adjusted diet cost also showed a positive association with FMI (β = 0.0354, 95% CI: 0.0001–0.0709, Pfor-trend = 0.01), BMISDS (β = 0.0200, 95% CI: 0.0006–0.0394, Pfor-trend = 0.002) and WHtR (β = 0.0010, 95% CI: 0.0003–0.0017, Pfor-trend = 0.02). Conclusions: nergy-adjusted diet cost was independently and positively associated with diet quality and obesity among Chinese school-aged children

Indocyanine Green-Loaded Mesoporous Silica Nanocomposite for Breast Cancer Imaging

Fluorescence imaging is essential for tumor staging and treatment decision-making. However, indocyanine green (ICG) imaging still faces challenges in terms of stability and targeting specificity despite improvements in penetration depth and the signal-to-noise ratio. To address these issues, a nanocomposite fluorescence probe called ICG@MSNs-PEG-Ab has developed. This probe involves coupling an anti-PD-L1 antibody to mesoporous silica nanoparticles (MSNs) loaded with ICG and coated with polyethylene glycol (PEG). The ICG@MSNs-PEG-Ab nanocomposite probe demonstrates excellent biocompatibility and emits strong and stable fluorescence in the near-infrared second region (NIR-II) when it is exposed to 808 nm laser irradiation. Importantly, it outperforms free ICG in terms of prolonged retention in the body and increased resistance to photodegradation. By combining passive targeting of the enhanced permeability and retention effect with active targeting of the interaction between the anti-PD-L1 antibody and antigen, ICG@MSNs-PEG-Ab selectively targets PD-L1-positive hormonal nude mice. This achievement enables high-performance NIR-II imaging, allowing for rapid and precise in vivo tumor identification with an impressive tumor-to-muscle contrast ratio of 3.94. This work provides valuable insights into the design of high-quality NIR-II fluorescence probes for accurate tumor diagnosis and targeted drug delivery

Data_Sheet_2_The effects of family environment cognition and its difference perceived by adolescents and their parents on the treatment effect of non-suicidal self-injury behaviors in adolescents: a 1-year prospective cohort study.doc

IntroductionFamily environment is the primary environment for adolescent growth and development, which is believed to have an important impact on the occurrence of non-suicidal self-injury (NSSI) behavior in adolescents. This study aimed to explore the effects of family environment cognition and cognitive differences perceived by adolescents and their parents on the treatment effects of NSSI in adolescents and to provide more potential perspectives for NSSI treatment.MethodsA one-year prospective longitudinal sub-cohort investigation was carried out among 199 adolescents engaged in NSSI and one of their important guardians from the Longitudinal Psychosomatic Disease Study (LoPDS). The NSSI behaviors of adolescents were evaluated at 3 months, 6 months and 1 year after enrollment. The family environment scale (FES) and NSSI Behavior Questionnaire were used as assessment tools for family environment and adolescents NSSI behaviors. Multiple linear regression was used to investigate the role of family environment perception difference in the treatment effect of adolescent NSSI.ResultsAfter one year of follow-up, the perceived self-injury impulse score in recent 2 weeks, self-injury impulse frequency in recent 2 weeks, total number of self-injury in recent 2 weeks decreased significantly. The higher the adolescent family cohesion (Beta: 1.130, 95% CI: 0.886,1.373; p=0.032), parental family expressiveness (Beta: 0.818, 95% CI: 0.375,1.260; p=0.037) and parental family active-recreational orientation score (Beta: 0.609, 95% CI: 0.236,0.981; p=0.048), the better the treatment effect. However, higher adolescent family conflict (Beta: -0.838, 95% CI: -1.377,-0.298; p=0.024) were associated with lower treatment outcomes. The greater the cognitive difference between parents and adolescents in family cohesion (Beta: -1.307, 95% CI: -2.074,-0.539; p=0.014) and family conflict(Beta: -0.665, 95% CI: -0.919,-0.410; p=0.037), the worse the therapeutic effect of NSSI might be.DiscussionThere were certain differences in the cognition of family relationships between parents and adolescents, and subjective family relationship cognition and cognitive differences had a significant effect on the treatment effect of NSSI in adolescents. Helping them identify the cause of cognitive differences and conducting systematic family therapy from the points of difference may be another perspective to improve the treatment effect of NSSI in adolescents.</p