4,111 research outputs found
Self-assembled dextrin nanogel as protein carrier : controlled release and biological activity of IL-10
Interleukin-10 (IL-10) is an anti-inflammatory cytokine, which active form is a non-covalent homodimer. Given the potential of IL-10 for application in various medical conditions, it is essential to develop systems for its effective delivery. In previous work, it has been shown that a dextrin nanogel effectively incorporated and
stabilized rIL10, enabling its release over time. In this work, the delivery system based on dextrin nanogels was further analyzed. The biocompatibility of the nanogel was comprehensively analyzed, through cytotoxicity (lactate dehydrogenase release, MTS,
Live and Dead) and genotoxicity (comet) assays. The release profile of rIL-10 and its biological activity were evaluated in vivo, using C57BL/6 mice. Although able to maintain a stable concentration of IL-10 for at least 4 hours in mice serum, the amount of protein released was rather low. Despite this, the amount of rIL-10 released from the complex was biologically active inhibiting TNF-Îą production, in vivo, by LPSchallenged mice.
In spite of the significant stabilization achieved using the nanogel, rIL-10 still denatures rather quickly. An additional effort is thus necessary to develop an effective delivery
system for this cytokine, able to release active protein over longer periods of time. Nevertheless, the good biocompatibility, the protein stabilization effect and the ability to perform as a carrier with controlled release suggest that self-assembled dextrin nanogels
may be useful protein delivery systems.Contract grant sponsor: Fundacao para a Ciencia e Tecnologia (FCT), PortugalContract grant number: SFRH/BD/27359/2006Contract grant sponsor: FCTContract grant number: PTDC/BIO/67160/2006; SUDOE-FEDERIMMUNONETSOE1/P1/E01
Hipotireoidismo e suas Implicaçþes no PerĂodo Gestacional: uma RevisĂŁo de Literatura
Hypothyroidism during pregnancy is a serious health concern, increasing the risk of complications such as miscarriage and neurointellectual disability in offspring. Careful regulation of thyroid hormones is crucial for optimal fetal development. This article is an integrative review that seeks to clarify thyroid disorders in pregnancy and their treatment options. 175 articles from the PubMed database were analyzed, limited to human studies published in English between 2018 and 2023. Eleven articles were selected for analysis. Fetal consumption of maternal thyroid hormone and physiological changes during pregnancy demand an increase in maternal secretion of this hormone. Iodine deficiency can cause thyroid dysfunction, resulting in serious complications in pregnancy and child development. Diagnosing hypothyroidism is challenging, especially in pregnant women. Serum TSH is used as an initial test, and levothyroxine therapy is recommended to prevent complications. Hypothyroidism in pregnancy requires careful regulation of thyroid hormones. Diagnostic complexity highlights the need to consider physiological and ethnic variations. Treatment recommendations vary between organizations, highlighting the need for consensus. Close monitoring and adjustments in therapy are essential, but challenges remain, highlighting the need for more research into treatment limits and the potential benefit of combination therapy to improve long- term quality of life.
Keywords: Pregnancy, Hypothyroidism, Thyroid Gland Disease.El hipotiroidismo durante el embarazo es un problema de salud grave que aumenta el riesgo de complicaciones como aborto espontĂĄneo y discapacidad neurointelectual en la descendencia. La regulaciĂłn cuidadosa de las hormonas tiroideas es crucial para el desarrollo fetal Ăłptimo. Este artĂculo es una revisiĂłn integradora que busca esclarecer los trastornos tiroideos en el embarazo y sus opciones de tratamiento. Se analizaron 175 artĂculos de la base de datos PubMed, limitados a estudios en humanos publicados en inglĂŠs entre 2018 y 2023. Se seleccionaron once artĂculos para el anĂĄlisis. El consumo fetal de hormona tiroidea materna y los cambios fisiolĂłgicos durante el embarazo exigen un aumento en la secreciĂłn materna de esta hormona. La deficiencia de yodo puede causar disfunciĂłn tiroidea, lo que resulta en complicaciones graves en el embarazo y el desarrollo infantil. Diagnosticar el hipotiroidismo es un desafĂo, especialmente en mujeres embarazadas. La TSH sĂŠrica se utiliza como prueba inicial y se recomienda el tratamiento con levotiroxina para prevenir complicaciones. El hipotiroidismo durante el embarazo requiere una regulaciĂłn cuidadosa de las hormonas tiroideas. La complejidad diagnĂłstica resalta la necesidad de considerar variaciones fisiolĂłgicas y ĂŠtnicas. Las recomendaciones de tratamiento varĂan entre organizaciones, lo que destaca la necesidad de consenso. La monitorizaciĂłn estrecha y los ajustes en la terapia son esenciales, pero persisten desafĂos, lo que destaca la necesidad de realizar mĂĄs investigaciones sobre los lĂmites del tratamiento y el beneficio potencial de la terapia combinada para mejorar la calidad de vida a largo plazo.
Palabras clave: Embarazo, Hipotiroidismo, Enfermedad de la GlĂĄndula Tiroides.O hipotireoidismo durante a gravidez pode ocasionar seĚrios problemas aĚ sauĚde, aumentando o risco de complicaçoĚes como aborto espontaĚneo e deficieĚncia neurointelectual na descendeĚncia. A regulaçaĚo cuidadosa dos hormoĚnios tireoidianos eĚ crucial para o desenvolvimento fetal ideal. Este artigo eĚ uma revisaĚo integrativa que busca esclarecer distuĚrbios tireoidianos na gravidez e suas opçoĚes de tratamento. Foram analisados 175 artigos da base PubMed, limitados a estudos em humanos publicados em ingleĚs entre 2018 e 2023. Onze artigos foram selecionados para a anaĚlise. O consumo fetal de hormoĚnio tireoidiano materno e alteraçoĚes fisioloĚgicas na gravidez demandam um aumento na secreçaĚo materna desse hormoĚnio. A deficieĚncia de iodo pode causar disfunçaĚo tireoidiana, resultando em complicaçoĚes graves na gravidez e no desenvolvimento infantil. O diagnoĚstico do hipotireoidismo eĚ desafiador, especialmente em mulheres graĚvidas. O TSH seĚrico eĚ usado como teste inicial, e a terapia com levotiroxina eĚ recomendada para prevenir complicaçoĚes. O hipotireoidismo na gravidez requer regulaçaĚo cuidadosa dos hormoĚnios tireoidianos. A complexidade diagnoĚstica destaca a necessidade de considerar variaçoĚes fisioloĚgicas e eĚtnicas. RecomendaçoĚes de tratamento variam entre organizaçoĚes, destacando a necessidade de consenso. O monitoramento rigoroso e ajustes na terapia saĚo essenciais, mas persistem desafios, evidenciando a necessidade de mais pesquisas sobre limites de tratamento e o potencial benefiĚcio da terapia combinada para melhorar a qualidade de vida a longo prazo.
Palavras-chave: Gravidez, Hipotireoidismo, Doenças da GlaĚndula Tireoide
Measurement of the t(t)over-bar production cross section in the dilepton channel in pp collisions at âs=8 TeV
The top-antitop quark (t (t) over bar) production cross section is measured in proton-proton collisions at root s = 8 TeV with the CMS experiment at the LHC, using a data sample corresponding to an integrated luminosity of 5.3 fb(-1). The measurement is performed by analysing events with a pair of electrons or muons, or one electron and one muon, and at least two jets, one of which is identified as originating from hadronisation of a bottom quark. The measured cross section is 239 +/- 2 (stat.) +/- 11 (syst.) +/- 6 (lum.) pb, for an assumed top-quark mass of 172.5 GeV, in agreement with the prediction of the standard model
Azimuthal anisotropy of charged particles at high transverse momenta in PbPb collisions at sqrt(s[NN]) = 2.76 TeV
The azimuthal anisotropy of charged particles in PbPb collisions at
nucleon-nucleon center-of-mass energy of 2.76 TeV is measured with the CMS
detector at the LHC over an extended transverse momentum (pt) range up to
approximately 60 GeV. The data cover both the low-pt region associated with
hydrodynamic flow phenomena and the high-pt region where the anisotropies may
reflect the path-length dependence of parton energy loss in the created medium.
The anisotropy parameter (v2) of the particles is extracted by correlating
charged tracks with respect to the event-plane reconstructed by using the
energy deposited in forward-angle calorimeters. For the six bins of collision
centrality studied, spanning the range of 0-60% most-central events, the
observed v2 values are found to first increase with pt, reaching a maximum
around pt = 3 GeV, and then to gradually decrease to almost zero, with the
decline persisting up to at least pt = 40 GeV over the full centrality range
measured.Comment: Replaced with published version. Added journal reference and DO
Compressed representation of a partially defined integer function over multiple arguments
In OLAP (OnLine Analitical Processing) data are analysed in an n-dimensional cube. The cube may be represented as a partially defined function over n arguments. Considering that often the function is not defined everywhere, we ask: is there a known way of representing the function or the points in which it is defined, in a more compact manner than the trivial one
Performance of CMS muon reconstruction in pp collision events at sqrt(s) = 7 TeV
The performance of muon reconstruction, identification, and triggering in CMS
has been studied using 40 inverse picobarns of data collected in pp collisions
at sqrt(s) = 7 TeV at the LHC in 2010. A few benchmark sets of selection
criteria covering a wide range of physics analysis needs have been examined.
For all considered selections, the efficiency to reconstruct and identify a
muon with a transverse momentum pT larger than a few GeV is above 95% over the
whole region of pseudorapidity covered by the CMS muon system, abs(eta) < 2.4,
while the probability to misidentify a hadron as a muon is well below 1%. The
efficiency to trigger on single muons with pT above a few GeV is higher than
90% over the full eta range, and typically substantially better. The overall
momentum scale is measured to a precision of 0.2% with muons from Z decays. The
transverse momentum resolution varies from 1% to 6% depending on pseudorapidity
for muons with pT below 100 GeV and, using cosmic rays, it is shown to be
better than 10% in the central region up to pT = 1 TeV. Observed distributions
of all quantities are well reproduced by the Monte Carlo simulation.Comment: Replaced with published version. Added journal reference and DO
Search for new physics with same-sign isolated dilepton events with jets and missing transverse energy
A search for new physics is performed in events with two same-sign isolated
leptons, hadronic jets, and missing transverse energy in the final state. The
analysis is based on a data sample corresponding to an integrated luminosity of
4.98 inverse femtobarns produced in pp collisions at a center-of-mass energy of
7 TeV collected by the CMS experiment at the LHC. This constitutes a factor of
140 increase in integrated luminosity over previously published results. The
observed yields agree with the standard model predictions and thus no evidence
for new physics is found. The observations are used to set upper limits on
possible new physics contributions and to constrain supersymmetric models. To
facilitate the interpretation of the data in a broader range of new physics
scenarios, information on the event selection, detector response, and
efficiencies is provided.Comment: Published in Physical Review Letter
Performance of CMS muon reconstruction in pp collision events at sqrt(s) = 7 TeV
The performance of muon reconstruction, identification, and triggering in CMS
has been studied using 40 inverse picobarns of data collected in pp collisions
at sqrt(s) = 7 TeV at the LHC in 2010. A few benchmark sets of selection
criteria covering a wide range of physics analysis needs have been examined.
For all considered selections, the efficiency to reconstruct and identify a
muon with a transverse momentum pT larger than a few GeV is above 95% over the
whole region of pseudorapidity covered by the CMS muon system, abs(eta) < 2.4,
while the probability to misidentify a hadron as a muon is well below 1%. The
efficiency to trigger on single muons with pT above a few GeV is higher than
90% over the full eta range, and typically substantially better. The overall
momentum scale is measured to a precision of 0.2% with muons from Z decays. The
transverse momentum resolution varies from 1% to 6% depending on pseudorapidity
for muons with pT below 100 GeV and, using cosmic rays, it is shown to be
better than 10% in the central region up to pT = 1 TeV. Observed distributions
of all quantities are well reproduced by the Monte Carlo simulation.Comment: Replaced with published version. Added journal reference and DO
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