Procjena citotoksičnog i genotoksičnog utjecaja suvremenih dentalnih materijala in vivo

Objectives: The aim of the study was to assess the biocompatibility of modern composite and amalgam dental fillings. Material and Methods: The research was conducted on 150 healthy patients between the ages of 10 and 20 who had amalgam and composite fillings between 6 and 12 months. Under in vivo conditions, a swab of buccal cells near the fillings was taken, and the cytotoxic and genotoxic impact of composite and amalgam fillings on these cells was analyzed using the extended micronucleus test (cytomeassay). Results: The results showed statistically significant differences between the groups of subjects with amalgam and composite fillings and subjects without fillings for the following parameters: number of micronuclei (p=0.006), number of buds (p<0.001), number of binuclear cells (p<0.001), number of nucleoplasmic bridges (p<0.001).The number of micronuclei was statistically significantly higher in the group of subjects with amalgam and composite fillings compared to the group without fillings. The results for nuclear buds, for the number of binuclear cells and the number of nucleoplasmic bridges showed that the group with amalgam fillings had a statistically significantly higher number of these changes compared to other groups.The results of the analysis of the relationship between the parameters of the micronucleus test and the number of amalgam and composite surfaces did not show statistically significant values. Parameters indicating cell cytotoxicity were not statistically significantly elevated in subjects with fillings.The results of the analysis of the influence of the patients’ lifestyle on the results of the micronucleus test showed statistically significant results for certain predictors (diagnostic X-ray radiation, coffee consumption, consumption of cooked, dried meat and baked food). Conclusion: Based on the results, it can be concluded that the buccal cells of subjects with amalgam fillings showed the highest degree of genotoxic changes, followed by those with composite fillings and the least buccal cells of patients without fillings.Svrha rada: Željela se procijeniti biokompatibilnost suvremenih kompozitnih i amalgamskih zubnih ispuna. Materijali i postupci: Istraživanje je provedeno na skupini od 150 zdravih pacijenata u dobi između 10 i 20 godina koji su imali amalgamske i kompozitne ispune starosti između 6 i 12 mjeseci. U uvjetima in vivo uzimao se bris bukalnih stanica u blizini ispuna te se primjenom proširenoga mikronukleusnoga testa (cytomeassay) analizirao citotoksični i genotoksični utjecaj kompozitnih i amalgamskih ispuna na te stanice. Rezultati: Rezultati su pokazali statistički značajne razlike između skupina ispitanika s amalgamskim i kompozitnim ispunima te ispitanika bez ispuna za sljedeće parametre: broj mikronukleusa (p = 0,006), broj pupova (p < 0,001), broj binuklearnih stanica (p < 0,001) i broj nukleoplazmatskih mostova (p < 0,001). Broj mikronukleusa bio je statistički značajno veći u skupini ispitanika s amalgamskim i kompozitnim ispunima u usporedbi sa skupinom bez ispuna. Rezultati za jezgrene pupove, za broj binuklearnih stanica i broj nukleoplazmatskih mostova pokazali su da je skupina s amalgamskim ispunima imala statistički značajno veći broj tih promjena u usporedbi s ostalim skupinama. Rezultati analize povezanosti parametara mikronukleusnog testa i broja amalgamskih te kompozitnih ploha nisu pokazali statistički značajne vrijednosti. Parametri koji upozoravaju na citotoksičnost stanica nisu bili statistički znatno povišeni kod ispitanika s ispunima. Rezultati analize utjecaja načina života pacijenata na ishode mikronukleusnog testa pokazali su statistički značajne rezultate za određene prediktore (dijagnostičko rendgensko zračenje, konzumacija kave, kuhanih i pečenih jela te suhomesnatih proizvoda). Zaključak: Na temelju rezultata može se zaključiti da su bukalne stanice ispitanika s amalgamskim ispunima pokazale najviši stupanj genotoksičnih promjena, zatim slijede one s kompozitnim ispunima, a najmanje su promjene zabilježene kod pacijenata bez ispuna

Centrality evolution of the charged-particle pseudorapidity density over a broad pseudorapidity range in Pb-Pb collisions at root s(NN)=2.76TeV

Peer reviewe

Oral health in childhood

According to American Dental Association (ADA), “oral health is a functional, structural, aesthetic, physiologic, and psychosocial state of well-being and is essential to an individual’s general health and quality of life” . Oral health education aims to avert diseases and put preventive measures in place. In order to control factors and avoid caries, preventive measures like: managing the bacterial flora in the mouth, educating people, fluoridating water, promoting healthy eating habits, and scheduling routine dental checkups are crucial. The goal is to influence the child’s systemic health in addition to their oral health by educating them about a proper diet. To avoid going to the dentist only when a tooth hurts, prevention should begin early. It is necessary to bring the child to the dental office to get used to the environment. The goal of prevention in childhood is to keep all teeth healthy in adulthood

Should the Application of Diode Laser Completely Replace Conventional Pulpotomy of Primary Teeth?

Nowadays, the use of lasers in dental medicine has become an effective approach for numerous restorative and soft tissue therapeutic procedures. The use of different types of lasers depends on how the tissue interacts with the laser light and the purpose of treatment. Although some studies show the benefits of laser pulpotomy compared to the conventional method of pulpotomy, clinical studies are lacking. Moreover, the material used for a definitive restoration of endodontically treated primary teeth can also affect the outcome of the therapy. Therefore, this prospective study aimed to compare the clinical use of the diode laser and the conventional method of vital pulpotomy, as well as the influence of the material chosen for a definitive restoration for the outcome of the procedure. Patients were divided into two groups: conventional pulpotomy and 980 nm diode laser. Each group was then divided into two subgroups according to the type of material for the final restoration. By comparing the success of the therapy according to clinical parameters depending on the material of the final restoration, the advantage of the composite material compared to the glass ionomer cement was noticed at 6 months checkup (p = 0.045). A strong positive correlation between the quality of the final restoration according to the United States Public Health Service (USPHS) criteria and the success of the clinical parameters was found in all investigated time points. There is no statistically significant difference between the diode laser and the conventional pulpotomy, however, the choice and quality of the final restoration significantly contribute to the outcome of therapy

Small Cell Lung Carcinoma: Current Diagnosis, Biomarkers, and Treatment Options with Future Perspectives

Small cell lung cancer (SCLC) is an aggressive malignancy characterized by rapid proliferation, early dissemination, acquired therapy resistance, and poor prognosis. Early diagnosis of SCLC is crucial since most patients present with advanced/metastatic disease, limiting the potential for curative treatment. While SCLC exhibits initial responsiveness to chemotherapy and radiotherapy, treatment resistance commonly emerges, leading to a five-year overall survival rate of up to 10%. New effective biomarkers, early detection, and advancements in therapeutic strategies are crucial for improving survival rates and reducing the impact of this devastating disease. This review aims to comprehensively summarize current knowledge on diagnostic options, well-known and emerging biomarkers, and SCLC treatment strategies and discuss future perspectives on this aggressive malignancy

Comprehensive molecular and clinical insights into non-small cell lung cancer transformation to small cell lung cancer with an illustrative case report

Histologic transformation to small cell lung cancer (tSCLC) is a rare but increasingly recognised mechanism of acquired resistance to tyrosine kinase inhibitors (TKI) in patients with epidermal growth factor receptor (EGFR)-positive non-small cell lung cancer (NSCLC). Beyond its acknowledged role in TKI resistance, histologic transformation to SCLC might be an important, yet under-recognised, mechanism of resistance in NSCLC treated with immunotherapy. Our review identified 32 studies that investigated tSCLC development in patients with EGFR-mutated NSCLC treated with TKI therapy and 16 case reports of patients treated with immunotherapy. It revealed the rarity of tSCLC, with a predominance of EGFR exon 19 mutations and limited therapeutic options and outcomes. Across all analysed studies in EGFR-mutated NSCLC treated with TKI therapy, the median time to tSCLC development was similar to 17 months, with a median overall survival of 10 months. Histologic transformation of EGFR-mutated NSCLC to SCLC is a rare, but challenging clinical problem with a poor prognosis. A small number of documented cases of tSCLC after immunotherapy highlight the need for rebiopsies at progression to diagnose this potential resistance mechanism. Further research is needed to better understand the mechanisms underlying this phenomenon and to develop more effective treatment strategies for patients with tSCLC

Possible Risk Factors for Dental Fear and Anxiety in Children Who Suffered Traumatic Dental Injury

Background: Children who undergo painful experiences such as traumatic dental injury (TDI) during their early years are more likely to be at an increased risk of developing dental fear and anxiety (DFA). The purpose of this study was to identify potential risk factors for DFA of these children. Methods: The study participants were 220 parents/caregivers and their children who experienced TDI. Their socio-demographic backgrounds were investigated with the modified WHO Oral Health Questionnaire for Children that included questions about parents’ knowledge and attitudes, while the DFA level was determined using the Children’s Fear Survey Schedule-Dental Subscale (CFSS-DS) and the Simplified Oral Hygiene Index (OHI-S Index) was used to assess oral hygiene status. Results: The confirmed risk factors are parental knowledge, female gender, and degree of oral hygiene and pain in the last three months, while age, type of TDI, presence of soft-tissue injury, and number of subjective complaints were not confirmed. The overall model predicted approximately 54% of variance in DFA, R2 = 0.545, F (4.215) = 64.28 p < 0.001. Conclusions: These findings emphasise the importance of addressing pain management, improving oral hygiene, and enhancing parental knowledge to mitigate DFA in children with TDIs

Relationship between Children’s and Parents’ Dental Anxiety: A Cross-Sectional Study on the Six European Countries

Background: The purpose of this study is to investigate the relationship between children’s and parents’ dental anxiety. Methods: 731 children of different ages and their parents from six European countries participated in this study. Dental anxiety was investigated through an online questionnaire, which consisted of general questions and the Corah Dental Anxiety Scale (CDAS), which is a questionnaire that measures respondents’ reactions on a 5-point scale for four different situations. Results: CDAS results were calculated for all children and their parents. A total of 12.5% of children from Croatia, 26.67% from Macedonia, 10.94% from Bosnia and Herzegovina, 20.31% from Montenegro, 23.08% from Slovenia and 16.10% from Serbia showed a high level of anxiety. The correlation between dental anxiety of parents and children was 0.4 (p < 0.01). Conclusions: Parents with negative experience from a dental office can have a bad effect on their child’s behaviour, which results in the creation of a non-active patient. Due to the clear and existing cause-and-effect relationship of dental anxiety in children and parents, it is extremely important to educate parents about the proper psychological approach to children in order to promote positive experiences from dental offices, as well as to emphasize the importance of regular visits to the dentist

Assessment of Cytotoxic and Genotoxic Effect of Modern Dental Materials in vivo

Objectives: The aim of the study was to assess the biocompatibility of modern composite and amalgam dental fillings. Material and Methods: The research was conducted on 150 healthy patients between the ages of 10 and 20 who had amalgam and composite fillings between 6 and 12 months. Under in vivo conditions, a swab of buccal cells near the fillings was taken, and the cytotoxic and genotoxic impact of composite and amalgam fillings on these cells was analyzed using the extended micronucleus test (cytomeassay). Results: The results showed statistically significant differences between the groups of subjects with amalgam and composite fillings and subjects without fillings for the following parameters: number of micronuclei (p=0.006), number of buds (p<0.001), number of binuclear cells (p<0.001), number of nucleoplasmic bridges (p<0.001).The number of micronuclei was statistically significantly higher in the group of subjects with amalgam and composite fillings compared to the group without fillings. The results for nuclear buds, for the number of binuclear cells and the number of nucleoplasmic bridges showed that the group with amalgam fillings had a statistically significantly higher number of these changes compared to other groups.The results of the analysis of the relationship between the parameters of the micronucleus test and the number of amalgam and composite surfaces did not show statistically significant values. Parameters indicating cell cytotoxicity were not statistically significantly elevated in subjects with fillings.The results of the analysis of the influence of the patients’ lifestyle on the results of the micronucleus test showed statistically significant results for certain predictors (diagnostic X-ray radiation, coffee consumption, consumption of cooked, dried meat and baked food). Conclusion: Based on the results, it can be concluded that the buccal cells of subjects with amalgam fillings showed the highest degree of genotoxic changes, followed by those with composite fillings and the least buccal cells of patients without fillings

Coherent J/ψ photoproduction in ultra-peripheral Pb–Pb collisions at √sNN=2.76 TeV

The ALICE Collaboration has made the first measurement at the LHC of J/ψ photoproduction in ultra-peripheral Pb–Pb collisions at sNN=2.76 TeV. The J/ψ is identified via its dimuon decay in the forward rapidity region with the muon spectrometer for events where the hadronic activity is required to be minimal. The analysis is based on an event sample corresponding to an integrated luminosity of about 55 μb−1. The cross section for coherent J/ψ production in the rapidity interval −3.6<y<−2.6 is measured to be dσJ/ψcoh/dy=1.00±0.18(stat)−0.26+0.24(syst) mb. The result is compared to theoretical models for coherent J/ψ production and found to be in good agreement with those models which include nuclear gluon shadowing