A Cdk1 phosphomimic mutant of MCAK impairs microtubule end recognition

The microtubule depolymerising kinesin-13, MCAK, is phosphorylated at residue T537 by Cdk1. This is the only known phosphorylation site within MCAK’s motor domain. To understand the impact of phosphorylation by Cdk1 on microtubule depolymerisation activity, we have investigated the molecular mechanism of the phosphomimic mutant T537E. This mutant significantly impairs microtubule depolymerisation activity and when transfected into cells causes metaphase arrest and misaligned chromosomes. We show that the molecular mechanism underlying the reduced depolymerisation activity of this phosphomimic mutant is an inability to recognise the microtubule end. The microtubule-end residence time is reduced relative to wild-type MCAK, whereas the lattice residence time is unchanged by the phosphomimic mutation. Further, the microtubule-end specific stimulation of ADP dissociation, characteristic of MCAK, is abolished by this mutation. Our data shows that T537E is unable to distinguish between the microtubule end and the microtubule lattice

NGC2180: a disrupting open cluster

The spatial dependence of luminosity and mass functions of evolved open clusters is discussed in this work using J and H 2MASS photometry. The target objects are the overlooked open cluster NGC2180 and the intermediate-age open cluster NGC3680. We conclude that, although in an advanced dynamical state (mass segregated), NGC3680 does not present strong signs of dissolution. On the other hand, NGC2180 presents flat, eroded LFs throughout its structure, indicating that in addition to mass segregation, Galactic tidal stripping has been effective in depleting this cluster of stars. Accordingly, NGC2180 may be the missing link between evolved open clusters and remnants. We study both clusters in the context of dynamical states estimated from diagnostic-diagrams involving photometric and structural parameters. Both clusters are dynamically evolved systems. In particular, NGC2180 is closer to open cluster remnants than NGC3680.Comment: 9 pages, 8 figure

Dissolving star cluster candidates

We present a list of 34 neglected entries from star cluster catalogues located at relatively high galactic latitudes ($|b| >$ 15$^{\circ}$) which appear to be candidate late stages of star cluster dynamical evolution. Although underpopulated with respect to usual open clusters, they still present a high number density contrast as compared to the galactic field. This was verified by means of (i) predicted model counts from different galactic subsystems in the same direction, and (ii) Guide Star Catalog equal solid angle counts for the object and surrounding fields. This suggests that the objects are physical systems, possibly star clusters in the process of disruption or their fossil remains. The sample will be useful for followup studies in view of verifying their physi cal nature.Comment: manuscript in LATEX with 7 pages, 7 figures .ps Accepted for Astronomy and Astrophysics main journa

A kinematic study of Open Clusters: implications for their origin

The Galactic population of open clusters provides an insight into star formation in the Galaxy. The open cluster catalogue by Dias et al.(2002b) is a rich source of data, including kinematic information. This large sample made it possible to carry out a systematic analysis of 481 open cluster orbits, using parameters based on orbit eccentricity and separation from the Galactic plane. These two parameters may be indicative of origin, and we find them to be correlated. We also find them to be correlated with metallicity, another parameter suggested elsewhere to be a marker for origin in that high values of any of these two parameters generally indicates a low metallicity ([Fe/H] Solar$<-$0.2 dex). The resulting analysis points to four open clusters in the catalogue being of extra-Galactic origin by impact of high velocity cloud on the disk: Berkeley21, 32, 99, and Melotte66, with a possible further four due to this origin (NGC2158, 2420, 7789, IC1311). A further three may be due to Galactic globular cluster impact on the disk i.e of internal Galactic origin (NGC6791, 1817, and 7044).Comment: 14 pages, 816 figures, accepted for publication in MNRAS 14-May-201

A photometric study of the old open clusters Berkeley 73, Berkeley 75 and Berkeley 25

CCD BVI photometry of the faint open clusters Berkeley~73, Berkeley~75 and Berkeley~25 are presented. The two latter are previously unstudied clusters to our knowledge. While Berkeley~73 is found to be of intermediate-age (about 1.5 Gyr old), Berkeley~75 and Berkeley~25 are two old clusters, with ages larger than 3.0 Gyr. We provide also estimates of the clusters size. Very interestingly, all these clusters turn out to lie far away from the Galactic Center, at $R_{GC} \geq$ 16 kpc, and quite high onto the Galactic plane, at $|Z_{\odot}| \geq 1.5$ kpc. They are therefore important targets to probe the properties of the structure of the Galaxy in this direction, where the Canis Major over-density has been recently discovered to be located.Comment: 8 pages, 10 figures, in press in A&

Hubble Space Telescope Observations of Element Abundances in Low-redshift Damped Lyman-alpha Galaxies and Implications for the Global Metallicity-Redshift Relation

Most models of cosmic chemical evolution predict that the mass-weighted mean interstellar metallicity of galaxies should rise with time from a low value $\sim 1/30$ solar at $z \sim 3$ to a nearly solar value at $z = 0$. In the absence of any selection effects, the damped Lyman-alpha absorbers (DLAs) in quasar spectra are expected to show such a rise in global metallicity. However, it has been difficult to determine whether or not DLAs show this effect, primarily because of the very small number of DLA metallicity measurements at low redshifts. In an attempt to put tighter constraints on the low-redshift end of the DLA metallicity-redshift relation, we have observed Zn II and Cr II lines in four DLAs at $0.09 < z < 0.52$, using the Space Telescope Imaging Spectrograph (STIS) onboard the Hubble Space Telescope (HST). These observations have provided the first constraints on Zn abundances in DLAs with $z < 0.4$. In all the three DLAs for which our observations offer meaningful constraints on the metallicity, the data suggest that the metallicities are much lower than the solar value. These results are consistent with recent imaging studies indicating that these DLAs may be associated with dwarf or low surface brightness galaxies. We combine our results with higher redshift data from the literature to estimate the global mean metallicity-redshift relation for DLAs. We find that the global mean metallicity shows at most a slow increase with decreasing redshift. ...(Please see the paper for the complete abstract).Comment: 56 pages, including 13 figures. Accepted for publication in the Astrophysical Journa

Old open clusters in the inner Galaxy: FSR1744, FSR89 and FSR31

We establish the nature and derive fundamental and structural parameters of the recently catalogued objects FSR1744, FSR89 and FSR31. This work intends to provide clues to constrain the Galactic tidal disruption efficiency, improve statistics of the open cluster parameter space, and better define their age-distribution function inside the Solar circle. Properties of the objects are investigated by means of 2MASS colour-magnitude diagrams and stellar radial density profiles built with field star decontaminated photometry. Diagnostic-diagrams for structural parameters are used to help disentangle dynamical from high-background effects affecting such centrally projected open clusters. FSR1744, FSR89 and FSR31 are Gyr-class OCs located at Galactocentric distances 4.0 - 5.6kpc. Compared to nearby OCs, they have small core and limiting radii. With respect to the small number of OCs observed in the inner Galaxy, the emerging scenario in the near-infrared favours disruption driven by dynamical evolution rather than observational limitations associated with absorption and/or high background levels. Internally, the main processes associated with the dynamical evolution are, e.g. mass loss by stellar evolution, mass segregation and evaporation. Externally they are, e.g. tidal stress from the disk and bulge, and interactions with giant molecular clouds. FSR1744, FSR89 and FSR31 have structural parameters consistent with their Galactocentric distances, in the sense that tidally induced effects may have accelerated the dynamical evolution.Comment: 12 pages and 13 figs; A&A, accepted, July 9, 200

What can carcass-based assessments tell us about the lifetime welfare status of pigs?

There is increasing interest in developing abattoir-based measures of farm animal welfare. It is important to understand the extent to which these measures reflect lifetime welfare status. The study aim was to determine whether lesions acquired during different production stages remain visible on the carcass, and the degree to which carcass-based measures may reflect broader health and welfare issues. 532 animals were assessed at 7, 9 and 10 weeks of age (early life, EL), and at 15 and 20 weeks of age (later life, LL) for tail lesions (TL), skin lesions (SL) and a number of health issues (HI) including lameness and coughing. Pigs were categorised according to when individual welfare issues occurred in the production process; ‘early life’ [EL], ‘later life’ [LL], ‘whole life’ [WL], or ‘uninjured’ (U) if showing no signs of a specific welfare issue on-farm. Following slaughter, carcasses were scored for tail length, tail lesions, and skin lesions and cold carcass weights (CCW) were obtained. Generalised linear, ordinal logistic and binary logistic fixed model procedures were carried out to examine the ability of TL, SL and HI lifetime categories to predict carcass traits. Pigs with TL in EL, LL and WL had higher carcass tail lesion scores than U pigs (P 0.05), also had shorter tails at slaughter than U pigs. In relation to TL scores, U pigs also had a higher cold carcass weight compared to LL and WL (P 0.05). Pigs with SL in EL, LL and WL had higher healed skin lesion scores on the carcass than U pigs (P 0.05). The current study shows that tail lesions and skin lesions, acquired at least 10 weeks before slaughter, remain evident on the carcass and consequently, may be useful as tools to assist in determining the lifetime welfare status of pigs. Low CCW was associated with tail lesions, supporting previous research suggesting that tail lesions have a negative impact on growth performance in pigs

The Sun was not born in M 67

Using the most recent proper-motion determination of the old, Solar-metallicity, Galactic open cluster M 67, in orbital computations in a non-axisymmetric model of the Milky Way, including a bar and 3D spiral arms, we explore the possibility that the Sun once belonged to this cluster. We have performed Monte Carlo numerical simulations to generate the present-day orbital conditions of the Sun and M 67, and all the parameters in the Galactic model. We compute 3.5 \times 10^5 pairs of orbits Sun-M 67 looking for close encounters in the past with a minimum distance approach within the tidal radius of M 67. In these encounters we find that the relative velocity between the Sun and M 67 is larger than 20 km/s. If the Sun had been ejected from M 67 with this high velocity by means of a three-body encounter, this interaction would destroy an initial circumstellar disk around the Sun, or disperse its already formed planets. We also find a very low probability, much less than 10^-7, that the Sun was ejected from M 67 by an encounter of this cluster with a giant molecular cloud. This study also excludes the possibility that the Sun and M 67 were born in the same molecular cloud. Our dynamical results convincingly demonstrate that M67 could not have been the birth cluster of our Solar System.Comment: Astronomical Journal accepted (35 pages, 9 figures

Brown dwarfs and very low mass stars in the Praesepe open cluster: a dynamically unevolved mass function?

[Abridged] In this paper, we present the results of a photometric survey to identify low mass and brown dwarf members of the old open cluster Praesepe (age of 590[+150][-120]Myr and distance of 190[+6.0][-5.8]pc) and use this to infer its mass function which we compare with that of other clusters. We have performed an optical (Ic-band) and near-infrared (J and Ks-band) photometric survey of Praesepe with a spatial coverage of 3.1deg^2. With 5sigma detection limits of Ic=23.4 and J=20.0, our survey is sensitive to objects with masses from about 0.6 to 0.05Msol. The mass function of Praesepe rises from 0.6Msol down to 0.1Msol and then turns-over at ~0.1Msol. The rise observed is in agreement with the mass function derived by previous studies, including a survey based on proper motion and photometry. Comparing our mass function with that for another open cluster with a similar age, the Hyades (age ~ 600Myr), we see a significant difference. Possible reasons are that dynamical evaporation has not influenced the Hyades and Praesepe in the same way, or that the clusters did not have the same initial mass function, or that dynamical interactions have modified the evolution of one or both clusters. Although a difference in the binary fractions of the clusters could cause the observed (i.e. system) mass functions to differ, measurements in the literature give no evidence for a significant difference in the binary fractions of the two clusters. Of our cluster candidates, six have masses predicted to be equal to or below the stellar/substellar boundary at 0.072Msol.Comment: 11 pages, 11 figures, accepted for publication in A&A. Higher resolution of Figures 2-3-4-5 in A&A published version. Revised version corrected for Englis