Insights into the development of Ixodes scapularis: a resource for research on a medically important tick species

Ticks (Acari: Ixodida) are arthropod ectoparasites dependent on a bloodmeal from a vertebrate host at each developmental stage for completion of their life cycle. This tick feeding cycle impacts animal health by causing damage to hides, secondary infections, immune reactions and diseases caused by transmission of pathogens. The genus Ixodes includes several medically important species that vector diseases, including granulocytic anaplasmosis and Lyme disease. I. scapularis, commonly called the black-legged or deer tick, is a medically-important tick species in North America and therefore was the first tick genome to be sequenced, thus serving as an important resource for tick research. This Primer focuses on the normal developmental cycle and laboratory rearing of I. scapularis. Definition of normal morphology, along with a consistent source of laboratory-reared I. scapularis, are fundamental for all aspects of future research, especially the effects of genetic manipulation and the evaluation of tick vaccine efficacy. Recent research important for the advancement of tick research, namely the development of tick cell culture systems for study of ticks and tick-borne pathogens, RNA interference for genetic manipulation of ticks and discovery of candidate antigens for development of tick vaccines, are briefly presented along with areas to target for future research.The preparation of this chapter was partially supported by the EU FP7 ANTIGONE project number 278976, and the Walter R. Sitlington Endowed Chair for Food Animal Research to K.M. Kocan.Peer Reviewe

Completing the puzzle of the 2004-2005 outburst in V0332+53: the brightening phase included

Analysis of the data obtained with the RXTE observatory during a powerful outburst of the X-ray pulsar V0332+53 in 2004-2005 is presented. Observational data covering the outburst brightening phase are analysed in detail for the first time. A comparison of source parameters and their evolution during the brightening and fading phases shows no evidence for any hysteresis behaviour. It is found that the dependences of the energy of the cyclotron absorption line on the luminosity during the brightening and fading phases are almost identical. The complete data sequence including the outburst brightening and fading phases makes it possible to impose the more stringent constraints on the magnetic field in the source. The pulse profile and pulsed fraction are studied as functions of the luminosity and photon energy.Comment: 9 pages, 10 figures, accepted for publication in MNRA

XMM-Newton observation of the persistent Be/NS X-ray binary pulsar RX J1037.5-5647 in a low luminosity state

The spectra of several X-ray binary pulsars display a clear soft excess, which in most cases can be described with a blackbody model, above the main power-law component. While in the high-luminosity sources it is usually characterized by low temperature (kT 100 km), in the two persistent and low-luminosity pulsars 4U 0352+309 and RX J0146.9+6121 this component has a high temperature (kT > 1 keV) and a smaller radius (R < 0.5 km), consistent with the estimated size of the neutron-star polar cap. Here we report on the timing and spectral analysis of RX J1037.5-5647, another low-luminosity persistent Be binary pulsar, based on the first XMM-Newton observation of this source. We have found a best-fit period P = 853.4(+/-0.2) s, that implies an average pulsar spin-up dP/dt ~ -2E-8 s/s in the latest decade. The estimated source luminosity is Lx ~ 10^34 erg/s, a value comparable to that of the other persistent Be binary pulsars and about one order of magnitude lower than in most of the previous measurements. The source spectrum can be described with a power law plus blackbody model, with kTbb = 1.26(+0.16/-0.09) keV and Rbb = 128(+13/-21) m, suggesting a polar-cap origin of this component. These results strengthen the hypothesis that, in addition to low luminosities and long periods, this class of sources is characterized also by common spectral propertiesComment: 9 pages, 8 figures, 2 tables. Accepted for publication by Astronomy and Astrophysic

XMM-Newton observation of the persistent Be/neutron-star system X Persei at a high-luminosity level

We report on the XMM-Newton observation of the HMXRB X Persei, the prototype of the persistent and low-luminosity Be/neutron star pulsars, which was performed on February 2003. The source was detected at a luminosity level of ~ 1.4x10^35 erg/s, which is the highest level of the latest three decades. The pulsation period has increased up to 839.3 s, thus confirming the overall spin-down of the NS detected in the previous observations. The folded light-curve has a complex structure, with features not observed at lower luminosities, and shows a significant energy dependence. The spectral analysis reveals the presence of a significant excess at low energies over the main power-law spectral component, which can be described by a black-body spectrum of high temperature (kT_BB ~ 1.5 keV) and small emitting region (R_BB ~ 340 m); its properties are consistent with a polar-cap origin. Phase-resolved spectroscopy shows that the emission spectrum varies along the pulse period, but it is not possible to prove whether the thermal component is pulsed or not.Comment: 8 pages, 8 figures, 4 tables. Accepted for publication by Astronomy & Astrophysic

Suzaku X-ray Spectra and Pulse Profile Variations during the Superorbital Cycle of LMC X-4

We present results from spectral and temporal analyses of Suzaku and RXTE observations of the high mass X-ray binary LMC X-4. Using the full 13 years of available RXTE/ASM data, we apply the ANOVA and Lomb normalized Periodogram methods to obtain an improved superorbital period measurement of 30.32 +/- 0.04 days. The phase-averaged X-ray spectra from Suzaku observations during the high state of the superorbital period can be modeled in the 0.6--50 keV band as the combination of a power-law with Gamma ~ 0.6 and a high-energy cutoff at ~ 25 keV, a blackbody with kT_BB ~ 0.18 keV, and emission lines from Fe K_alpha, O VIII, and Ne IX (X Lyalpha). Assuming a distance of 50 kpc, The source has luminosity L_X ~ 3 x 10^38 ergs s^-1 in the 2--50 keV band, and the luminosity of the soft (blackbody) component is L_BB ~ 1.5 x 10^37 ergs s^-1. The energy resolved pulse profiles show single-peaked soft (0.5-1 keV) and hard (6-10 keV) pulses but a more complex pattern of medium (2-10 keV) pulses; cross-correlation of the hard with the soft pulses shows a phase shift that varies between observations. We interpret these results in terms of a picture in which a precessing disk reprocesses the hard X-rays and produces the observed soft spectral component, as has been suggested for the similar sources Her X-1 and SMC X-1.Comment: 13 emulateapj pages, 11 figures, 4 tables; accepted for publication in Ap

A model for cyclotron resonance scattering features

(abbreviated version of the abstract) We study the physics of cyclotron line formation in the high-energy spectra of accreting X-ray pulsars using Monte Carlo methods, assuming that the line-forming region is a low-density electron plasma in a sub-critical magnetic field. We investigate the dependence of the shape of the fundamental line on angle, geometry, optical depth and temperature. We also discuss variations of the line ratios for non-uniform magnetic fields. These numerical predictions for the line profiles are linked to results from observational data analysis using an XSPEC model based on the Monte Carlo simulations. We apply this model to observational data from RXTE and INTEGRAL. The predicted strong emission wings of the fundamental cyclotron feature are not found in observational data, hinting at a bottom illuminated slab geometry for line formation.Comment: 16 pages, 15 figures, Astron. Astrophys. (in press

GX 301-2 as seen by Integral

We present observations of the High Mass X-ray Binary GX 301-2 taken during the Galactic Plane Scan (GPS) with Integral following our RXTE observations (Kreykenbohm et al. 2004). The optical companion of GX 301-2 is the B1Ia+ hypergiant Wray 977 with a luminosity of 1.3 x 10^6 Lsun and a mass of ~48 Msun making the system one of the most massive X-ray binaries known. The system was observed 24 times during the GPS thus covering many orbital phases including the pre-periastron flare. The source is clearly detected in all Integral instruments in most pointings. The derived X-ray light curves show strong variability on all timescales. Depending on the orbital phase of the pointings, the luminosity changes by factor of more than 10 during the pre-periastron flare compared to other pointings. Furthermore, broad band spectra using all instruments available on Integral are compared with data taken by the RXTE instruments PCA and HEXTE (using the same data as in Kreykenbohm et al. 2004).Comment: accepted for publication in the proceedings of the 5th Integral Workshop (The INTEGRAL Universe, Munich, 16-20 February 2004

Strong outburst activity of the X-ray pulsar X Persei during 2001-2011

We present results of a comprehensive analysis of the X-ray pulsar X Persei over the period 1996 to 2011, encompassing the quite low state and subsequent strong outburst activity. Using data from the RXTE and Swift observatories we detected several consecutive outbursts, during which the source luminosity increased by a factor of ~5 up to L_x~1.2x10^{35} erg/s. Previously the source was observed in a high state only once. The source spectrum in a standard energy band (4-25 keV) is independent of the flux change and can be described by a model that includes both thermal and non-thermal components. With the help of the INTEGRAL observatory data we registered the highly significant cyclotron absorption line in the source spectrum and, for the first time, significantly detected a hard X-ray emission from the pulsar up to ~160 keV. We also report drastic changes of the pulse period during the outburst activity: a long episode of spin-down was changed to spin-up with a rate of Pdot/P = -(3-5)x10^{-4} yr^{-1}, that is several times higher than previous rates of spin-up and spin-down. To search for a correlation between the X-ray and optical lightcurves we took data from the AAVSO International Database. No significant correlation between optical and X-ray fluxes at any time lag from dozens of days to years was found.Comment: 8 pages, 6 figures, accepted for publication in MNRA

Is there a highly magnetized neutron star in GX 301-2?

We present the results of an in-depth study of the long-period X-ray pulsar GX 301-2. Using archival data of INTEGRAL, RXTE ASM, and CGRO BATSE, we study the spectral and timing properties of the source. Comparison of our timing results with previously published work reveals a secular decay of the orbital period at a rate of \simeq -3.25 \times 10^{-5} d yr^{-1}, which is an order of magnitude faster than for other known systems. We argue that this is probably result either of the apsidal motion or of gravitational coupling of the matter lost by the optical companion with the neutron star, although current observations do not allow us to distinguish between those possibilities. We also propose a model to explain the observed long pulse period. We find that a very strong magnetic field B \sim 10^{14} G can explain the observed pulse period in the framework of existing models for torques affecting the neutron star. We show that the apparent contradiction with the magnetic field strength B_{CRSF} \sim 4 \times 10^{12} G derived from the observed cyclotron line position may be resolved if the line formation region resides in a tall accretion column of height \sim 2.5 - 3 R_{NS}. The color temperature measured from the spectrum suggests that such a column may indeed be present, and our estimates show that its height is sufficient to explain the observed cyclotron line position.Comment: 10 pages, 7 figures, accepted for publication in Astronomy and Astrophysics. Referee comments are implemented

Broadband Observations of the Be/X-ray Binary Pulsar RX J0440.9+4431: Discovery of a Cyclotron Absorption Line

We report the results of an analysis of data obtained with the INTEGRAL, Swift and RXTE observatories during the 2010 April and September outbursts of the X-ray pulsar RX J0440.9+4431. The temporal and spectral properties of the pulsar in a wide energy band (0.6-120 keV) were studied for the first time. We discovered a ~32 keV cyclotron resonant scattering feature in the source spectrum, that allowed us to estimate the magnetic field strength of the neutron star as B~3.2 x 10^12 G. The estimate of the magnetic field strength was confirmed by a comprehensive analysis of the noise power spectrum of the source. Based on the recurrence time between Type I outbursts the orbital period of the binary system can be estimated as ~155 days. We have shown that the pulse profile has a sinusoidal-like single-peaked shape and has practically no dependence on the source luminosity or energy band.Comment: 8 pages, 6 figures, accepted for publication in MNRA