DNA-PAINT MINFLUX nanoscopy

MINimal fluorescence photon FLUXes (MINFLUX) nanoscopy, providing photon-efficient fluorophore localizations, has brought about three-dimensional resolution at nanometer scales. However, by using an intrinsic on–off switching process for single fluorophore separation, initial MINFLUX implementations have been limited to two color channels. Here we show that MINFLUX can be effectively combined with sequentially multiplexed DNA-based labeling (DNA-PAINT), expanding MINFLUX nanoscopy to multiple molecular targets. Our method is exemplified with three-color recordings of mitochondria in human cells

Mentoring of newly qualified teachers. A bilateral comparison between Germany and Finland

Mentoring stellt in der Begleitung von in den Beruf einsteigenden Lehrpersonen eine international gängige Praxis dar. Ausgehend von theoretischen und empirisch fundierten Annahmen wird in diesem Beitrag der Ansatz der practice architectures genutzt, um Formen des Mentorings in Deutschland und Finnland im Hinblick auf länderspezifische Verständnisse vom Berufseinstieg von Lehrpersonen und deren Professionalisierung zu untersuchen. (DIPF/Orig.)Internationally, mentoring is a well-recognized practice in supporting newly qualified teachers. Based on theoretical and empirical understanding, this paper compares mentoring practices in two countries: Germany and Finland. The theoretical framework of practice architectures is used to explore mentoring as an expression of a particular view on teacher induction and professionalization of teachers. (DIPF/Orig.

Engagement and Learning from a team-based mini-project in mechatronic engineering

We outline our experiences with hidden and unsignposted learning by us and by our students arising from a team-based project activity in a 3rd-year undergraduate engineering module in the general Mechatronics area. We discuss the hidden learning achieved in areas such as team communications, team management, problem-solving skills, and communication through the media of student-produced video and presentations, as well as technical engineering reports. We describe the enablement of student reflection on their learning and its benefits and use these reflections to evidence various aspects of their learning. The work is situated within the literature on innovations and quality of STEM education

PERICLES Deliverable 4.3:Content Semantics and Use Context Analysis Techniques

The current deliverable summarises the work conducted within task T4.3 of WP4, focusing on the extraction and the subsequent analysis of semantic information from digital content, which is imperative for its preservability. More specifically, the deliverable defines content semantic information from a visual and textual perspective, explains how this information can be exploited in long-term digital preservation and proposes novel approaches for extracting this information in a scalable manner. Additionally, the deliverable discusses novel techniques for retrieving and analysing the context of use of digital objects. Although this topic has not been extensively studied by existing literature, we believe use context is vital in augmenting the semantic information and maintaining the usability and preservability of the digital objects, as well as their ability to be accurately interpreted as initially intended.PERICLE

Prevalence and predictors of video game addiction: a study based on a national representative sample of gamers

Video gaming has become a popular leisure activity in many parts of the world, and an increasing number of empirical studies examine the small minority that appears to develop problems as a result of excessive gaming. This study investigated prevalence rates and predictors of video game addiction in a sample of gamers, randomly selected from the National Population Registry of Norway (N =3389). Results showed there were 1.4 % addicted gamers, 7.3 % problem gamers, 3.9 % engaged gamers, and 87.4 % normal gamers. Gender (being male) and age group (being young) were positively associated with addicted-, problem-, and engaged gamers. Place of birth (Africa, Asia, South- and Middle America) were positively associated with addicted- and problem gamers. Video game addiction was negatively associated with conscientiousness and positively associated with neuroticism. Poor psychosomatic health was positively associated with problem- and engaged gaming. These factors provide insight into the field of video game addiction, and may help to provide guidance as to how individuals that are at risk of becoming addicted gamers can be identified

Optical techniques for 3D surface reconstruction in computer-assisted laparoscopic surgery

One of the main challenges for computer-assisted surgery (CAS) is to determine the intra-opera- tive morphology and motion of soft-tissues. This information is prerequisite to the registration of multi-modal patient-specific data for enhancing the surgeon’s navigation capabilites by observ- ing beyond exposed tissue surfaces and for providing intelligent control of robotic-assisted in- struments. In minimally invasive surgery (MIS), optical techniques are an increasingly attractive approach for in vivo 3D reconstruction of the soft-tissue surface geometry. This paper reviews the state-of-the-art methods for optical intra-operative 3D reconstruction in laparoscopic surgery and discusses the technical challenges and future perspectives towards clinical translation. With the recent paradigm shift of surgical practice towards MIS and new developments in 3D opti- cal imaging, this is a timely discussion about technologies that could facilitate complex CAS procedures in dynamic and deformable anatomical regions

Worldtube excision method for intermediate-mass-ratio inspirals: scalar-field model in 3+1 dimensions

Binary black hole simulations become increasingly more computationally expensive with smaller mass ratios, partly because of the longer evolution time, and partly because the lengthscale disparity dictates smaller time steps. The program initiated by Dhesi et al. (arXiv:2109.03531) explores a method for alleviating the scale disparity in simulations with mass ratios in the intermediate astrophysical range ($10^{-4} \lesssim q \lesssim 10^{-2}$), where purely perturbative methods may not be adequate. A region ("worldtube") much larger than the small black hole is excised from the numerical domain, and replaced with an analytical model approximating a tidally deformed black hole. Here we apply this idea to a toy model of a scalar charge in a fixed circular geodesic orbit around a Schwarzschild black hole, solving for the massless Klein-Gordon field. This is a first implementation of the worldtube excision method in full 3+1 dimensions. We demonstrate the accuracy and efficiency of the method, and discuss the steps towards applying it for evolving orbits and, ultimately, in the binary black-hole scenario. Our implementation is publicly accessible in the SpECTRE numerical relativity code.Comment: 19 pages, 10 figure

A scalable elliptic solver with task-based parallelism for the SpECTRE numerical relativity code

Elliptic partial differential equations must be solved numerically for many problems in numerical relativity, such as initial data for every simulation of merging black holes and neutron stars. Existing elliptic solvers can take multiple days to solve these problems at high resolution and when matter is involved, because they are either hard to parallelize or require a large amount of computational resources. Here we present a new solver for linear and non-linear elliptic problems that is designed to scale with resolution and to parallelize on computing clusters. To achieve this we employ a discontinuous Galerkin discretization, an iterative multigrid-Schwarz preconditioned Newton-Krylov algorithm, and a task-based parallelism paradigm. To accelerate convergence of the elliptic solver we have developed novel subdomain-preconditioning techniques. We find that our multigrid-Schwarz preconditioned elliptic solves achieve iteration counts that are independent of resolution, and our task-based parallel programs scale over 200 million degrees of freedom to at least a few thousand cores. Our new code solves a classic black-hole binary initial-data problem faster than the spectral code SpEC when distributed to only eight cores, and in a fraction of the time on more cores. It is publicly accessible in the next-generation SpECTRE numerical relativity code. Our results pave the way for highly-parallel elliptic solves in numerical relativity and beyond.Comment: 24 pages, 18 figures. Results are reproducible with the ancillary input file

Correlated intense X-ray and TeV activity of Mrk~501 in 1998 June

We present exactly simultaneous X-ray and TeV monitoring with {\it RXTE} and HEGRA of the TeV blazar Mrk 501 during 15 days in 1998 June. After an initial period of very low flux at both wavelengths, the source underwent a remarkable flare in the TeV and X-ray energy bands, lasting for about six days and with a larger amplitude at TeV energies than in the X-ray band. At the peak of the TeV flare, rapid TeV flux variability on sub-hour timescales is found. Large spectral variations are observed at X-rays, with the 3--20 keV photon index of a pure power law continuum flattening from $\Gamma=2.3$ to $\Gamma=1.8$ on a timescale of 2--3 days. This implies that during the maximum of the TeV activity, the synchrotron peak shifted to energies $\gtrsim 50$ keV, a behavior similar to that observed during the longer-lasting, more intense flare in 1997 April. The TeV spectrum during the flare is described by a power law with photon index $\Gamma=1.9$ and an exponential cutoff at $\sim$ 4 TeV; an indication for spectral softening during the flare decay is observed in the TeV hardness ratios. Our results generally support a scenario where the TeV photons are emitted via inverse Compton scattering of ambient seed photons by the same electron population responsible for the synchrotron X-rays. The simultaneous spectral energy distributions (SEDs) can be fit with a one-zone synchrotron-self Compton model assuming a substantial increase of the magnetic field and the electron energy by a factor of 3 and 10, respectively.Comment: Accepted for publication in ApJ, Part