1,101 research outputs found

    Evaluation of different methods for determining the angle of attack on wind turbine blades with CFD results under axial inflow conditions

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    This work presents an investigation on different methods for the calculation of the angle of attack and the underlying induced velocity on wind turbine blades using data obtained from three-dimensional Computational Fluid Dynamics (CFD). Several methods are examined and their advantages, as well as shortcomings, are presented. The investigations are performed for two 10MW reference wind turbines under axial inflow conditions, namely the turbines designed in the EU AVATAR and INNWIND.EU projects. The results show that the evaluated methods are in good agreement with each other at the mid-span, though some deviations are observed at the root and tip regions of the blades. This indicates that CFD results can be used for the calibration of induction modeling for Blade Element Momentum (BEM) tools. Moreover, using any of the proposed methods, it is possible to obtain airfoil characteristics for lift and drag coefficients as a function of the angle of attack.Comment: This manuscript is Accepted at at Renewable Energy journal- online 13 March 2018 under the CC-BY-NC-ND 4.0 licens

    Demethylation of DNA by purified chick embryo 5-methylcytosine-DNA glycosylase requires both protein and RNA

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    We have previously purified and characterized a 5-methylcytosine (5-MeC)-DNA glycosylase from 12 day old chick embryos [Jost,J.P. et al. (1995) J. Biol. Chem. 270, 9734-9739]. The activity of the purified enzyme is abolished upon treatment with proteinase K and ribonuclease A. RNA copurifies with 5-MeC-DNA glycosylase activity throughout all chromatographic steps and preparative gel electrophoresis. RNA with a length of ∼300-500 nucleotides was isolated from the gel purified enzyme. Upon extensive treatment with proteinase K, the gel eluted and labeled RNA did not show any significant change in molecular mass. The purified RNA incubated alone or in the presence of Mg2+ and deoxyribonucleotide phosphates had no 5-MeC-DNA glycosylase or demethylating activities. However, activity of 5-MeC-DNA glycosylase could be restored when the purified RNA was incubated with the inactive protein, free of RN

    Primary structure and functional expression of a cyclic nucleotidegated channel from rabbit aorta

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    Sequences specific for cyclic nucleotide-gated channels (CNG channels) have been amplified by PCR from cDNA of heart, aorta, sinoatrial node, cerebellum, C-cells and kidney. The complete amino acid sequence of a CNG channel from rabbit aorta has been deduced by cloning and sequence analysis of the cDNA. Synthetic RNA derived from this cDNA induces the formation of a functional CNG channel in Xenopus oocytes

    Generalized inverse mean curvature flows in spacetime

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    Motivated by the conjectured Penrose inequality and by the work of Hawking, Geroch, Huisken and Ilmanen in the null and the Riemannian case, we examine necessary conditions on flows of two-surfaces in spacetime under which the Hawking quasilocal mass is monotone. We focus on a subclass of such flows which we call uniformly expanding, which can be considered for null as well as for spacelike directions. In the null case, local existence of the flow is guaranteed. In the spacelike case, the uniformly expanding condition leaves a 1-parameter freedom, but for the whole family, the embedding functions satisfy a forward-backward parabolic system for which local existence does not hold in general. Nevertheless, we have obtained a generalization of the weak (distributional) formulation of this class of flows, generalizing the corresponding step of Huisken and Ilmanen's proof of the Riemannian Penrose inequality.Comment: 21 pages, 1 figur

    Controls on the diurnal streamflow cycles in two subbasins of an alpine headwater catchment

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    In high-altitude alpine catchments, diurnal streamflow cycles are typically dominated by snowmelt or ice melt. Evapotranspiration-induced diurnal streamflow cycles are less observed in these catchments but might happen simultaneously. During a field campaign in the summer 2012 in an alpine catchment in the Swiss Alps (Val Ferret catchment, 20.4 km2, glaciarized area: 2%), we observed a transition in the early season from a snowmelt to an evapotranspiration-induced diurnal streamflow cycle in one of two monitored subbasins. The two different cycles were of comparable amplitudes and the transition happened within a time span of several days. In the second monitored subbasin, we observed an ice melt-dominated diurnal cycle during the entire season due to the presence of a small glacier. Comparisons between ice melt and evapotranspiration cycles showed that the two processes were happening at the same times of day but with a different sign and a different shape. The amplitude of the ice melt cycle decreased exponentially during the season and was larger than the amplitude of the evapotranspiration cycle which was relatively constant during the season. Our study suggests that an evapotranspiration-dominated diurnal streamflow cycle could damp the ice melt-dominated diurnal streamflow cycle. The two types of diurnal streamflow cycles were separated using a method based on the identification of the active riparian area and measurement of evapotranspiration

    Calibration of TCCON column-averaged CO2: the first aircraft campaign over European TCCON sites

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    The Total Carbon Column Observing Network (TCCON) is a ground-based network of Fourier Transform Spectrometer (FTS) sites around the globe, where the column abundances of CO2, CH4, N2O, CO and O2 are measured. CO2 is constrained with a precision better than 0.25% (1-σ). To achieve a similarly high accuracy, calibration to World Meteorological Organization (WMO) standards is required. This paper introduces the first aircraft calibration campaign of five European TCCON sites and a mobile FTS instrument. A series of WMO standards in-situ profiles were obtained over European TCCON sites via aircraft and compared with retrievals of CO2 column amounts from the TCCON instruments. The results of the campaign show that the FTS measurements are consistently biased 1.1% ± 0.2% low with respect to WMO standards, in agreement with previous TCCON calibration campaigns. The standard a priori profile for the TCCON FTS retrievals is shown to not add a bias. The same calibration factor is generated using aircraft profiles as a priori and with the TCCON standard a priori. With a calibration to WMO standards, the highly precise TCCON CO2 measurements of total column concentrations provide a suitable database for the calibration and validation of nadir-viewing satellite

    Trapped surfaces and symmetries

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    We prove that strictly stationary spacetimes cannot contain closed trapped nor marginally trapped surfaces. The result is purely geometric and holds in arbitrary dimension. Other results concerning the interplay between (generalized) symmetries and trapped submanifolds are also presented.Comment: 9 pages, no figures. Final corrected version to appear in Class. Quantum Gra

    Foisonnement de l'innovation agricole : quelques exemples d'initiatives en élevage herbivore

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    Les témoignages rassemblés pour illustrer le foisonnement des innovations agricoles émanent d'acteurs différents (agriculteurs, recherche, développement) mais sont tous caractérisés par des approches plutôt systémiques et des dynamiques de co-conception. Les thèmes abordés concernent la production (valorisation des surfaces avec des cultures dérobées, sélection d'espèce prairiales locales), l'appropriation de résultats de recherche (amélioration de la gestion des prairies), la conception d'itinéraires techniques (solutions pour limiter les pertes d'azote en rotation prairie - prairie), l'évaluation de systèmes (repérer des pratiques innovantes en mobilisant des principes de l'agroécologie) mais aussi l'amélioration des conditions de travail et la formation (communication « intergénérationnelle » entre des paysans herbagers et des élèves)

    Evolution of the gut microbiome following acute HIV-1 infection

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    Background: In rhesus macaques, simian immunodeficiency virus infection is followed by expansion of enteric viruses but has a limited impact on the gut bacteriome. To understand the longitudinal effects of HIV-1 infection on the human gut microbiota, we prospectively followed 49 Mozambican subjects diagnosed with recent HIV-1 infection (RHI) and 54 HIV-1-negative controls for 9–18 months and compared them with 98 chronically HIV-1- infected subjects treated with antiretrovirals (n = 27) or not (n = 71). Results: We show that RHI is followed by increased fecal adenovirus shedding, which persists during chronic HIV-1 infection and does not resolve with ART. Recent HIV-1 infection is also followed by transient non-HIV-specific changes in the gut bacterial richness and composition. Despite early resilience to change, an HIV-1-specific signature in the gut bacteriome—featuring depletion of Akkermansia, Anaerovibrio, Bifidobacterium, and Clostridium—previously associated with chronic inflammation, CD8+ T cell anergy, and metabolic disorders, can be eventually identified in chronically HIV-1-infected subjects. Conclusions: Recent HIV-1 infection is associated with increased fecal shedding of eukaryotic viruses, transient loss of bacterial taxonomic richness, and long-term reductions in microbial gene richness. An HIV-1-associated microbiome signature only becomes evident in chronically HIV-1-infected subjects

    Atmospheric GHG measurements onboard Voluntary Observing Ships - approaches for improved atmospheric sampling

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    Autonomous systems measuring the partial pressure of CO2 (pCO2) in surface waters on commercial carrier ships (Voluntary Observing Ship, VOS), which allows for high spatiotemporal data coverage, are a major component of the Ocean Thematic Centre (OTC) data stream. Currently, ICOS operates lines in the Atlantic, North Sea and the Baltic. All lines are determining pCO2 by measuring CO2 in air that has been equilibrated with seawater. As part of the European H2020 project RINGO (https://www.icos-ri.eu/ringo), we are evaluating the possibility of using VOS to expand the atmospheric network. We will provide technical solutions for three different settings and approaches, and assess the added value for the atmospheric observation network. Two systems are designed as stand-alone modules for continuous atmospheric CO2 and CH4 measurements, following the technological requirements defined by the ATC, and will be operated in the Baltic (high anthropogenic influence) and on a line between France and Brazil (clean marine air, large temperature and humidity gradient). A second approach is using the existing instrumentation for seawater measurements (North Atlantic), which we aim to improve in order to make these measurements usable for the atmospheric research community. This is an effort that connects the ocean research community with the Central Analytical Laboratories (CAL; testing an extended range of standard gases, providing flask sampling opportunity), the Atmospheric Thematic Centre (ATC; work on data streams that can be digested by the ATC system), and the modelling community (identifying useful sampling strategies). Here we present a status update of the ongoing work, which is a joined effort of the atmospheric and ocean community within ICOS and relying on the expertise of both fields
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