Tannic extract efficiency combined or not with boric acid on protection of Ceiba pentandra wood against xylophagous termite

Dentre os métodos que vêm sendo testados para minimizar a lixiviação de compostos de boro na madeira, destaca-se sua combinação com taninos vegetais. Aos taninos vegetais é atribuída a durabilidade natural da madeira de algumas espécies, indicando sua potencialidade como preservativo natural. Neste estudo, avaliou-se o rendimento de taninos condensados provenientes da casca de Mimosa tenuiflora em extração realizada com água destilada, comparando-o ao da extração envolvendo a inclusão de sulfito de sódio, assim como a eficiência de extratos tânicos sulfitados, combinados ou não com ácido bórico, na melhoria da resistência da madeira de Ceiba pentandra ao térmita xilófago Nasutitermes corniger, por meio de ensaio de preferência alimentar. Extrato tânico obtido com a inclusão de sulfito de sódio à água teve melhor rendimento em taninos condensados. De maneira geral, a impregnação da madeira com o extrato tânico sulfitado proporcionou o mesmo comportamento quando comparada à aplicação do ácido bórico, sendo os melhores resultados verificados quando ambos foram utilizados conjuntamente. No entanto, depois de aplicado na madeira, o extrato tânico foi lixiviado e não favoreceu a redução da lixiviação do ácido bórico. Apesar da potencialidade do uso do extrato tânico ao cupim xilófago, estudos adicionais fazem-se necessários.Palavras-chave: Biodeterioração da madeira; térmitas xilófagos; Nasutitermes corniger. AbstractTannic extract efficiency combined or not with boric acid on protection of Ceiba pentandra wood against xylophagous termite. Among the methods tested to reduce the leaching of boron compounds in wood, it can be highlighted their combination with vegetable tannins. It is attributed the natural durability of some species of wood to vegetable tannins, pointing to their potentiality as natural wood preservatives. This research evaluated the efficiency of condensed tannins from bark of Mimosa tenuiflora extract with distilled water, compared to another extract added of sodium sulfite. It also evaluated the efficiency of sulfited tannin extracts, combined or not with boric acid in order to improve the resistance of Ceiba pentandra against the xylophagous termite Nasutitermes corniger by feeding test. Tannic extract obtained by adding sodium sulfite to distilled water revealed better yield compared to condensed tannin extraction. In general, impregnation of wood with tannin extract presented the same behavior as compared to application of boric acid, with best results observed when both them were simultaneously used. However, after applied to wood, tannin extracted was leachable and it did not favor the boric acid leaching. Despite the potential use of tannic extract against the xylophagous termite, additional studies are required for a best evaluation.Keywords: Wood biodeterioration; xilophogous termites; Nasutitermes corniger.AbstractAmong the methods tested to reduce the leaching of boron compounds in wood, it can be highlighted their combination with vegetable tannins. It is attributed the natural durability of some species of wood to vegetable tannins, pointing to their potentiality as natural wood preservatives. This research evaluated the efficiency of condensed tannins from bark of Mimosa tenuiflora extract with distilled water, compared to another extract added of sodium sulfite. It also evaluated the efficiency of sulfited tannin extracts, combined or not with boric acid in order to improve the resistance of Ceiba pentandra against the xylophagous termite Nasutitermes corniger by feeding test. Tannic extract obtained by adding sodium sulfite to distilled water revealed better yield compared to condensed tannin extraction. In general, impregnation of wood with tannin extract presented the same behavior as compared to application of boric acid, with best results observed when both them were simultaneously used. However, after applied to wood, tannin extracted was leachable and it did not favor the boric acid leaching. Despite the potential use of tannic extract against the xylophagous termite, additional studies are required for a best evaluation.Keywords: Wood biodeterioration; xilophogous termites; Nasutitermes corniger

Optimizing sampling design to deal with mist-net avoidance in Amazonian birds and bats

Mist netting is a widely used technique to sample bird and bat assemblages. However, captures often decline with time because animals learn and avoid the locations of nets. This avoidance or net shyness can substantially decrease sampling efficiency. We quantified the day-to-day decline in captures of Amazonian birds and bats with mist nets set at the same location for four consecutive days. We also evaluated how net avoidance influences the efficiency of surveys under different logistic scenarios using re-sampling techniques. Net avoidance caused substantial declines in bird and bat captures, although more accentuated in the latter. Most of the decline occurred between the first and second days of netting: 28% in birds and 47% in bats. Captures of commoner species were more affected. The numbers of species detected also declined. Moving nets daily to minimize the avoidance effect increased captures by 30% in birds and 70% in bats. However, moving the location of nets may cause a reduction in netting time and captures. When moving the nets caused the loss of one netting day it was no longer advantageous to move the nets frequently. In bird surveys that could even decrease the number of individuals captured and species detected. Net avoidance can greatly affect sampling efficiency but adjustments in survey design can minimize this. Whenever nets can be moved without losing netting time and the objective is to capture many individuals, they should be moved daily. If the main objective is to survey species present then nets should still be moved for bats, but not for birds. However, if relocating nets causes a significant loss of netting time, moving them to reduce effects of shyness will not improve sampling efficiency in either group. Overall, our findings can improve the design of mist netting sampling strategies in other tropical areas.We thank the Brazilian CNPq and Ministério do Meio Ambiente for research permits. João Valsecchi, Helder Queiróz, Miriam Marmontel, Pedro Santos, Jerry Tavares dos Santos, several field assistants, and the staff of the Instituto de Desenvolvimento Sustentável Mamirauá provided invaluable help with planning, logistics and field work. We also thank L. Reino, C. Miranda and N. Castanheira for help during field work. We are grateful to the communities of ASDR for their hospitality. Monique MacKenzie kindly helped with the GEE analyses. Dr. Mark Brigham made valuable comments and suggestions that helped to improve an earlier version of the manuscript.publishe

Nova emigração portuguesa: perfis, integração e expetativas

Este texto apresenta os principais resultados de um projeto de investigação, intitulado Regresso ao futuro: a nova emigração e a relação com a sociedade portuguesa (REMIGR), financiado pela FCT, que decorreu entre 2013 e 2015. O principal objetivo do projeto foi compreender a dimensão e características dos novos movimentos de emigração portuguesa, destacando, em especial, as relações que os novos emigrantes mantêm com o país de origem. Baseado numa estratégia metodológica mista, o estudo incluiu a recolha de estatísticas nacionais e internacionais, inquérito por questionário online e em papel, complementado por um conjunto de entrevistas a especialistas e representantes institucionais. Os países de destino escolhidos foram Reino Unido, França e Luxemburgo, na União Europeia, e Angola, Moçambique e Brasil, fora da Europa.info:eu-repo/semantics/publishedVersio

Nova emigração portuguesa: Perfis, integração e expetativas

Este texto apresenta os principais resultados de um projeto de investigação, intitulado Regresso ao futuro: a nova emigraçãoearelação com a sociedade portuguesa (REMIGR), financiado pela FCT, que decorreu entre 2013 e 2015. O principal objetivo do projeto foi compreender a dimensão e características dos novos movimentos de emigração portuguesa, destacando, em especial, as relações que os novos emigrantes mantêm com o país de origem. Baseado numa estratégia metodológica mista, o estudo incluiu a recolha de estatísticas nacionais e internacionais, inquérito por questionário online e em papel, complementado por um conjunto de entrevistas a especialistas e representantes institucionais. Os países de destino escolhidos foram Reino Unido, França e Luxemburgo, na União Europeia, e Angola, Moçambique e Brasil, fora da Europa.info:eu-repo/semantics/publishedVersio

Nova emigração portuguesa: Perfis, integração e expetativas

Este texto apresenta os principais resultados de um projeto de investigação, intitulado Regresso ao futuro: a nova emigração e a relação com a sociedade portuguesa (REMIGR), financiado pela FCT, que decorreu entre 2013 e 2015. O principal objetivo do projeto foi compreender a dimensão e características dos novos movimentos de emigração portuguesa, destacando, em especial, as relações que os novos emigrantes mantêm com o país de origem. Baseado numa estratégia metodológica mista, o estudo incluiu a recolha de estatísticas nacionais e internacionais, inquérito por questionário online e em papel, complementado por um conjunto de entrevistas a especialistas e representantes institucionais. Os países de destino escolhidos foram Reino Unido, França e Luxemburgo, na União Europeia, e Angola, Moçambique e Brasil, fora da Europa

OCORRÊNCIA DE CASOS DE RAIVA EM ANIMAIS SILVESTRES NO MUNICÍPIO DE CAICÓ-RN NO PERÍODO DE 2014 A 2019

Human health is closely linked to the health of the environment and animals, and it is extremely important to consider this relationship in decision-making that imply positive changes in social determinants of health. In this context, rabies is a zoonosis that has occurred in the municipality of Caicó-RN and has placed the city among the first in number of cases in wild animals in the state. This study aimed to report the occurrence of cases of rabies in wild animals in this municipality, from January 2014 to July 2019. A cross-sectional and descriptive epidemiological study of the type of time series, retrospective and quantitative, was carried out. Secondary data provided by LACEN / RN and CCZ regarding rabies casuistry in wild animals captured through passive rabies surveillance and subjected to Direct Immunofluorescence and Intracerebral Inoculation in Mice tests were used. 26 cases of rabies in wild animals were confirmed, 21 cases in bats (80.76%) and 5 in foxes (19.24%). Only these two wild species have been positively diagnosed for the disease. A wild variant commonly found in foxes, has been identified in a dog. The confirmation of positive cases of rabies in wild animals indicates that the circulation of the rabies virus exists in the municipality of Caicó, calling attention to the risk of exposure of domestic animals and the population. In view of this, it is of utmost importance that the public authorities subsidize the operation of surveillance services to prevent the spread of the disease.A saúde humana está intimamente ligada à saúde do ambiente e dos animais, sendo de extrema importância a consideração dessa relação nas tomadas de decisão que impliquem em mudanças positivas nos determinantes sociais de saúde. Nesse contexto, a raiva é uma zoonose que tem ocorrido no município de Caicó/RN e tem colocado a cidade entre as primeiras em número de casos em animais silvestres do estado. O presente trabalho objetivou relatar a ocorrência de casos de raiva em animais silvestres nesse município, no período de janeiro de 2014 a julho de 2019. Realizou-se um estudo epidemiológico transversal e descritivo do tipo de série temporal, retrospectivo e quantitativo. Foram utilizados dados secundários, fornecidos pelo LACEN/RN e CCZ, referentes à casuística de raiva em animais silvestres capturados através da vigilância passiva da raiva e submetidos a provas de Imunofluorescência Direta e Inoculação Intracerebral em Camundongos. Foram confirmados 26 casos de raiva em animais silvestres, sendo 21 casos em morcegos (80,76%) e 5 em raposas (19,24%). Apenas essas duas espécies silvestres, morcegos e raposas, foram diagnosticadas positivamente para a doença. Uma variante silvestre comumente encontrada em raposas foi identificada em um cão. A confirmação de casos positivos de raiva em animais silvestres indica que existe a circulação do vírus rábico no município de Caicó, chamando atenção para o risco de exposição de animais domésticos e da população. Diante disso, é de suma importância que o poder público subsidie o funcionamento dos serviços de vigilância para evitar a propagação da doença

Conflictos socioambientales y alternativas de la sociedad civil

Los conflictos socioambientales se multiplican en todo el mundo. Giran en torno a la contaminación industrial, las actividades mineras, el cambio de uso de suelo, la deforestación, la construcción de presas, la introducción de semillas genéticamente modificadas, el mal manejo de desechos sólidos y la privatización de tierra, agua y biodiversidad, entre otros. ¿Cuáles son las causas subyacentes a estos conflictos? ¿Quiénes son los protagonistas? ¿Cuáles son sus demandas, propuestas y estrategias? ¿En qué medida han contribuido a proteger o sanear el medio ambiente? ¿Hay conflictos locales que se convierten en glocales con la intervención de la sociedad civil? Estas interrogantes se abordan en este libro, que recoge inventarios de conflictos ambientales en México, en especial en Jalisco.ITESO, A.C

Casos de estudio en México y Latinoamérica

Ante la presencia de conflictos sociales y territoriales, las comunidades organizadas buscan estrategias de solución y confrontación. Es el estudio de dichos movimientos, lo que motiva a la publicación de éste libro: Respuestas comunitarias ante conflictos ambientales. Casos de estudio en México y Latinoamérica, reúne catorce textos que analizan las respuestas sociales y documentan la acción colectiva de comunidades que se han organizado para autogestionar soluciones ante conflictos territoriales, económicos y ambientales, en su propio entorno. Las aportaciones de investigadores y activistas, desde enfoques teóricos y metodológicos particulares, exponen casos de estudio sobre organizaciones formales e informales que se han conformado para afrontar los retos que representan proyectos productivos como fábricas cementeras, mineras, puertos, productores de energía, entre otros. Los enfoques analíticos tocan también temas nodales en el estudio de la acción colectiva como la ética, el despojo, los derechos humanos y las estrategias de comunicación y visibilización. Este material, que reúne estudios hechos en distintos lugares de México y Latinoamérica, es un compendio de métodos de investigación y un acercamiento al estudio de los movimientos sociales.UAEMEX, CONACyT, SE

Targeted sequencing identifies novel variants in common and rare MODY genes

Background: Maturity-onset diabetes of the young (MODY) is a form of monogenic diabetes with autosomal dominant inheritance. To date, mutations in 11 genes have been frequently associated with this phenotype. In Brazil, few cohorts have been screened for MODY, all using a candidate gene approach, with a high prevalence of undiagnosed cases (MODY-X). Methods: We conducted a next-generation sequencing target panel (tNGS) study to investigate, for the first time, a Brazilian cohort of MODY patients with a negative prior genetic analysis. One hundred and two patients were selected, of which 26 had an initial clinical suspicion of MODY-GCK and 76 were non-GCK MODY. Results: After excluding all benign and likely benign variants and variants of uncertain significance, we were able to assign a genetic cause for 12.7% (13/102) of the probands. Three rare MODY subtypes were identified (PDX1/NEUROD1/ABCC8), and eight variants had not been previously described/mapped in genomic databases. Important clinical findings were evidenced in some cases after genetic diagnosis, such as MODY-PDX1/HNF1B. Conclusion: A multiloci genetic approach allowed the identification of rare MODY subtypes, reducing the large percentage of MODY-X in Brazilian cases and contributing to a better clinical, therapeutic, and prognostic characterization of these rare phenotypes

CIGB-300, a synthetic peptide-based drug that targets the CK2 phosphoaceptor domain. Translational and clinical research

CK2 represents an oncology target scientifically validated. However, clinical research with inhibitors of the CK2-mediated phosphorylation event is still insufficient to recognize it as a clinically validated target. CIGB-300, an investigational peptide-based drug that targets the phosphoaceptor site, binds to a CK2 substrate array in vitro but mainly to B23/nucleophosmin in vivo. The CIGB-300 proapoptotic effect is preceded by its nucleolar localization, inhibition of the CK2-mediated phosphorylation on B23/nucleophosmin and nucleolar disassembly. Importantly, CIGB-300 shifted a protein array linked to apoptosis, ribosome biogenesis, cell proliferation, glycolisis, and cell motility in proteomic studies which helped to understand its mechanism of action. In the clinical ground, CIGB-300 has proved to be safe and well tolerated in a First-in-Human trial in women with cervical malignancies who also experienced signs of clinical benefit. In a second Phase 1 clinical trial in women with cervical cancer stage IB2/II, the MTD and DLT have been also identified in the clinical setting. Interestingly, in cervical tumors the B23/nucleophosmin protein levels were significantly reduced after CIGB-300 treatment at the nucleus compartment. In addition, expanded use of CIGB-300 in case studies has evidenced antitumor activity when administered as compassional option. Collectively, our data outline important clues on translational and clinical research from this novel peptide-based drug reinforcing its perspectives to treat cancer and paving the way to validate CK2 as a promising target in oncology.Fil: Perea, Silvio E.. Center for Genetic Engineering and Biotechnology; CubaFil: Baladron, Idania. Center for Genetic Engineering and Biotechnology; CubaFil: Garcia, Yanelda. Center for Genetic Engineering and Biotechnology; CubaFil: Perera, Yasser. Center for Genetic Engineering and Biotechnology; CubaFil: Lopez, Adlin. Center for Genetic Engineering and Biotechnology; CubaFil: Soriano, Jorge L.. Center for Genetic Engineering and Biotechnology; Cuba. General Hospital ‘‘Hermanos Ameijeiras’; CubaFil: Batista, Noyde. Center for Genetic Engineering and Biotechnology; Cuba. General Hospital ‘‘Hermanos Ameijeiras’; CubaFil: Palau, Aley. Center for Genetic Engineering and Biotechnology; Cuba. General Hospital ‘‘Hermanos Ameijeiras’; CubaFil: Hernández, Ignacio. Center for Genetic Engineering and Biotechnology; CubaFil: Farina, Hernán Gabriel. Universidad Nacional de Quilmes; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Garcia, Idrian. Center for Genetic Engineering and Biotechnology; CubaFil: Gonzalez, Lidia. Center for Genetic Engineering and Biotechnology; CubaFil: Gil, Jeovanis. Center for Genetic Engineering and Biotechnology; CubaFil: Rodriguez, Arielis. Center for Genetic Engineering and Biotechnology; CubaFil: Solares, Margarita. Center for Genetic Engineering and Biotechnology; CubaFil: Santana, Agueda. Center for Genetic Engineering and Biotechnology; CubaFil: Cruz, Marisol. Center for Genetic Engineering and Biotechnology; CubaFil: Lopez, Matilde. Center for Genetic Engineering and Biotechnology; CubaFil: Valenzuela, Carmen. Center for Genetic Engineering and Biotechnology; CubaFil: Reyes, Osvaldo. Center for Genetic Engineering and Biotechnology; CubaFil: López Saura, Pedro A.. Center for Genetic Engineering and Biotechnology; CubaFil: González, Carlos A.. Center for Genetic Engineering and Biotechnology; CubaFil: Diaz, Alina. Center for Genetic Engineering and Biotechnology; CubaFil: Castellanos, Lila. Center for Genetic Engineering and Biotechnology; CubaFil: Sanchez, Aniel. Center for Genetic Engineering and Biotechnology; CubaFil: Betancourt, Lazaro. Center for Genetic Engineering and Biotechnology; CubaFil: Besada, Vladimir. Center for Genetic Engineering and Biotechnology; CubaFil: González, Luis J.. Center for Genetic Engineering and Biotechnology; CubaFil: Garay, Hilda. Center for Genetic Engineering and Biotechnology; CubaFil: Gómez, Roberto. Center for Genetic Engineering and Biotechnology; CubaFil: Gomez, Daniel Eduardo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Quilmes; ArgentinaFil: Alonso, Daniel Fernando. Universidad Nacional de Quilmes; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Perrin, Phillipe. No especifíca;Fil: Renualt, Jean Yves. No especifíca;Fil: Sigman, Hugo. No especifíca;Fil: Herrera, Luis. Center for Genetic Engineering and Biotechnology; CubaFil: Acevedo, Boris. Center for Genetic Engineering and Biotechnology; Cub