Development of DNA Based Active Macro–Materials for Biology and Medicine: A Review

DNA was first discovered as the carrier of genetic information for the majority of the known living organisms, encoding the secret of life. Its delicate design based upon double helical structure and base pairing offers a stable and reliable media for storing hereditary codes, laying the foundation for the central dogma (Watson et al. 2003). The impact of this molecule is far reaching into scientific community and our society, as manifested in many fields, for instance, forensics (Budowle et al. 2003), besides medicine. To date, a great deal of research effort has been directed towards understandin

Probing the interstellar medium and star formation of the Most Luminous Quasar at z=6.3

We report new IRAM/PdBI, JCMT/SCUBA-2, and VLA observations of the ultraluminous quasar SDSSJ010013.02+280225.8 (hereafter, J0100+2802) at z=6.3, which hosts the most massive supermassive black hole (SMBH) of 1.24x10^10 Msun known at z>6. We detect the [C II] 158 $\mu$m fine structure line and molecular CO(6-5) line and continuum emission at 353 GHz, 260 GHz, and 3 GHz from this quasar. The CO(2-1) line and the underlying continuum at 32 GHz are also marginally detected. The [C II] and CO detections suggest active star formation and highly excited molecular gas in the quasar host galaxy. The redshift determined with the [C II] and CO lines shows a velocity offset of ~1000 km/s from that measured with the quasar Mg II line. The CO (2-1) line luminosity provides direct constraint on the molecular gas mass which is about (1.0+/-0.3)x10^10 Msun. We estimate the FIR luminosity to be (3.5+/-0.7)x10^12 Lsun, and the UV-to-FIR spectral energy distribution of J0100+2802 is consistent with the templates of the local optically luminous quasars. The derived [C II]-to-FIR luminosity ratio of J0100+2802 is 0.0010+/-0.0002, which is slightly higher than the values of the most FIR luminous quasars at z~6. We investigate the constraint on the host galaxy dynamical mass of J0100+2802 based on the [C II] line spectrum. It is likely that this ultraluminous quasar lies above the local SMBH-galaxy mass relationship, unless we are viewing the system at a small inclination angle.Comment: 19 pages, 4 figures, published by the Astrophysical Journal, minimal changes in acknowledgement to match the published versio

SCUBA2 High Redshift Bright Quasar Survey: Far-infrared Properties and Weak-line Features

We present a submillimetre continuum survey ('SCUBA2 High rEdshift bRight quasaR surveY', hereafter SHERRY) of 54 high redshift quasars at $5.6<z<6.9$ with quasar bolometric luminosities in a range of (0.2$-$$5)\times10^{14}\,L_{\odot}$, using the Submillimetre Common-User Bolometer Array-2 (SCUBA2) on the James Clerk Maxwell Telescope. About 30% (16/54) of the sources are detected with a typical 850$\mu$m rms sensitivity of 1.2 $\rm mJy\,beam^{-1}$ ($S\rm _{\nu,850\,\mu m} = 4$-5 mJy, at $>3.5\sigma$). The new SHERRY detections indicate far-infrared (FIR) luminosities of $\rm 3.5\times10^{12}$ to $\rm 1.4\times10^{13}$ $L_{\odot}$, implying extreme star formation rates of 90 to 1060 $M_{\odot}$ yr$^{-1}$ in the quasar host galaxies. Compared with $z =$ 2$-$5 samples, the FIR luminous quasars ($L_{\rm FIR} > 10^{13}\,L_{\odot}$) are more rare at $z \sim 6$. The optical/near-infrared (NIR) spectra of these objects show 11% (6/54) of the sources have weak Ly$\alpha$, emission line features, which may relate to different sub-phases of the central active galactic nuclei (AGNs). Our SCUBA2 survey confirms the trend reported in the literature that quasars with submillimeter detections tend to have weaker ultraviolet (UV) emission lines compared to quasars with nondetections. The connection between weak UV quasar line emission and bright dust continuum emission powered by massive star formation may suggest an early phase of AGN-galaxy evolution, in which the broad line region is starting to develop slowly or is shielded from the central ionization source, and has unusual properties such as weak line features or bright FIR emission.Comment: 28 pages, 10 figures, published in Ap

(SHERRY) JCMT-SCUBA2 High Redshift Bright Quasar Survey -- II: the environment of z~6 quasars in sub-millimeter band

The formation of the first supermassive black holes is expected to have occurred in some most pronounced matter and galaxy overdensities in the early universe. We have conducted a sub-mm wavelength continuum survey of 54 $z\sim6$ quasars using the Submillimeter Common-User Bolometre Array-2 (SCUBA2) on the James Clerk Maxwell Telescope (JCMT) to study the environments around $z \sim 6$ quasars. We identified 170 submillimeter galaxies (SMGs) with above 3.5$\sigma$ detections at 450 or 850 \um\, maps. Their FIR luminosities are 2.2 - 6.4 $\times$ 10$^{12} L_{\odot}$, and star formation rates are $\sim$ 400 - 1200 M$_{\odot}$ yr$^{-1}$. We also calculated the SMGs differential and cumulative number counts in a combined area of $\sim$ 620 arcmin$^2$. To a $4\sigma$ detection (at $\sim$ 5.5 mJy), SMGs overdensity is $0.68^{+0.21}_{-0.19}$($\pm0.19$), exceeding the blank field source counts by a factor of 1.68. We find that 13/54 quasars show overdensities (at $\sim$ 5.5 mJy) of $\delta_{SMG}\sim$ 1.5 - 5.4. The combined area of these 13 quasars exceeds the blank field counts with the overdensity to 5.5 mJy of \dsmg $\sim$ $2.46^{+0.64}_{-0.55}$($\pm0.25$) in the regions of $\sim$ 150 arcmin$^2$. However, the excess is insignificant on the bright end (e.g., 7.5 mJy). We also compare results with previous environmental studies of Lyman alpha emitters (LAEs) and Lyman-Break Galaxies (LBGs) on a similar scale. Our survey presents the first systematic study of the environment of quasars at $z\sim6$. The newly discovered SMGs provide essential candidates for follow-up spectroscopic observations to test whether they reside in the same large-scale structures as the quasars and search for protoclusters at an early epoch.Comment: 12 pages, 8 figures and 2 tables, accepted for publication in Ap

Computational design of transmembrane pores.

Transmembrane channels and pores have key roles in fundamental biological processes1 and in biotechnological applications such as DNA nanopore sequencing2-4, resulting in considerable interest in the design of pore-containing proteins. Synthetic amphiphilic peptides have been found to form ion channels5,6, and there have been recent advances in de novo membrane protein design7,8 and in redesigning naturally occurring channel-containing proteins9,10. However, the de novo design of stable, well-defined transmembrane protein pores that are capable of conducting ions selectively or are large enough to enable the passage of small-molecule fluorophores remains an outstanding challenge11,12. Here we report the computational design of protein pores formed by two concentric rings of α-helices that are stable and monodisperse in both their water-soluble and their transmembrane forms. Crystal structures of the water-soluble forms of a 12-helical pore and a 16-helical pore closely match the computational design models. Patch-clamp electrophysiology experiments show that, when expressed in insect cells, the transmembrane form of the 12-helix pore enables the passage of ions across the membrane with high selectivity for potassium over sodium; ion passage is blocked by specific chemical modification at the pore entrance. When incorporated into liposomes using in vitro protein synthesis, the transmembrane form of the 16-helix pore-but not the 12-helix pore-enables the passage of biotinylated Alexa Fluor 488. A cryo-electron microscopy structure of the 16-helix transmembrane pore closely matches the design model. The ability to produce structurally and functionally well-defined transmembrane pores opens the door to the creation of designer channels and pores for a wide variety of applications

A Genome Wide Association Study Identifies Common Variants Associated with Lipid Levels in the Chinese Population

Plasma lipid levels are important risk factors for cardiovascular disease and are influenced by genetic and environmental factors. Recent genome wide association studies (GWAS) have identified several lipid-associated loci, but these loci have been identified primarily in European populations. In order to identify genetic markers for lipid levels in a Chinese population and analyze the heterogeneity between Europeans and Asians, especially Chinese, we performed a meta-analysis of two genome wide association studies on four common lipid traits including total cholesterol (TC), triglycerides (TG), low-density lipoprotein cholesterol (LDL) and high-density lipoprotein cholesterol (HDL) in a Han Chinese population totaling 3,451 healthy subjects. Replication was performed in an additional 8,830 subjects of Han Chinese ethnicity. We replicated eight loci associated with lipid levels previously reported in a European population. The loci genome wide significantly associated with TC were near DOCK7, HMGCR and ABO; those genome wide significantly associated with TG were near APOA1/C3/A4/A5 and LPL; those genome wide significantly associated with LDL were near HMGCR, ABO and TOMM40; and those genome wide significantly associated with HDL were near LPL, LIPC and CETP. In addition, an additive genotype score of eight SNPs representing the eight loci that were found to be associated with lipid levels was associated with higher TC, TG and LDL levels (P = 5.52×10-16, 1.38×10-6 and 5.59×10-9, respectively). These findings suggest the cumulative effects of multiple genetic loci on plasma lipid levels. Comparisons with previous GWAS of lipids highlight heterogeneity in allele frequency and in effect size for some loci between Chinese and European populations. The results from our GWAS provided comprehensive and convincing evidence of the genetic determinants of plasma lipid levels in a Chinese population

Disparities and risks of sexually transmissible infections among men who have sex with men in China: a meta-analysis and data synthesis.

BACKGROUND: Sexually transmitted infections (STIs), including Hepatitis B and C virus, are emerging public health risks in China, especially among men who have sex with men (MSM). This study aims to assess the magnitude and risks of STIs among Chinese MSM. METHODS: Chinese and English peer-reviewed articles were searched in five electronic databases from January 2000 to February 2013. Pooled prevalence estimates for each STI infection were calculated using meta-analysis. Infection risks of STIs in MSM, HIV-positive MSM and male sex workers (MSW) were obtained. This review followed the PRISMA guidelines and was registered in PROSPERO. RESULTS: Eighty-eight articles (11 in English and 77 in Chinese) investigating 35,203 MSM in 28 provinces were included in this review. The prevalence levels of STIs among MSM were 6.3% (95% CI: 3.5-11.0%) for chlamydia, 1.5% (0.7-2.9%) for genital wart, 1.9% (1.3-2.7%) for gonorrhoea, 8.9% (7.8-10.2%) for hepatitis B (HBV), 1.2% (1.0-1.6%) for hepatitis C (HCV), 66.3% (57.4-74.1%) for human papillomavirus (HPV), 10.6% (6.2-17.6%) for herpes simplex virus (HSV-2) and 4.3% (3.2-5.8%) for Ureaplasma urealyticum. HIV-positive MSM have consistently higher odds of all these infections than the broader MSM population. As a subgroup of MSM, MSW were 2.5 (1.4-4.7), 5.7 (2.7-12.3), and 2.2 (1.4-3.7) times more likely to be infected with chlamydia, gonorrhoea and HCV than the broader MSM population, respectively. CONCLUSION: Prevalence levels of STIs among MSW were significantly higher than the broader MSM population. Co-infection of HIV and STIs were prevalent among Chinese MSM. Integration of HIV and STIs healthcare and surveillance systems is essential in providing effective HIV/STIs preventive measures and treatments. TRIAL REGISTRATION: PROSPERO NO: CRD42013003721

Performance of the CMS Cathode Strip Chambers with Cosmic Rays

The Cathode Strip Chambers (CSCs) constitute the primary muon tracking device in the CMS endcaps. Their performance has been evaluated using data taken during a cosmic ray run in fall 2008. Measured noise levels are low, with the number of noisy channels well below 1%. Coordinate resolution was measured for all types of chambers, and fall in the range 47 microns to 243 microns. The efficiencies for local charged track triggers, for hit and for segments reconstruction were measured, and are above 99%. The timing resolution per layer is approximately 5 ns