O processo de formação profissional nos semi-internatos : a percepção do acadêmico de enfermagem da Universidade Federal do Pará, Brasil

Avaliou-se o processo de formação profissional nos semi-internatos do Curso de Graduação em Enfermagem da Universidade Federal do Pará, a partir da percepção dos discentes. Utilizou-se uma abordagem quali-quantitativa por meio de estudo de caso, com análise documental Projeto Pedagógico - PP e das ementas do semi-internato e construção de um instrumento atitudinal do Tipo Likert. Concluiu-se que as competências de atenção a saúde e comunicação, o trabalho docente, a relação docente/discente e a articulação teoria-prática têm uma percepção positiva entre os discentes. Contudo observa-se uma percepção mais crítica quanto à oferta de cenários de prática em diferentes níveis de atenção, aspecto de grande relevância para a formação profissional em saúde

CP-Violation and Baryogenesis in The Low Energy Minimal Supersymmetric Standard Model

In the context of the minimal supersymmetric extension of the Standard Model the effect of a realistic wall profile is studied. It has been recently showed that in the presence of light stops the electroweak scale phase transition can be strong enough for baryogenesis. In the presence of non-trivial CP-violating phases of left-handed mixing terms and Higgsino mass, the largest $n_B/s$ is created when Higgsino and gaugino mass parameters are degenerate, $\mu = M_2$. In the present paper we show that realistic wall profiles suppress the generated baryon number of the universe, so that quite a stringent bound |\sin\phi_\mu | \gsim 0.2 for $\mu$-phase $\phi_\mu$ can be inferred.Comment: 8 pages(LaTex), 2 figure

Induction of nitric oxide synthase in rat immune complex glomerulonephritis

Induction of nitric oxide synthase in rat immune complex glomerulonephritis. Nitric oxide (NO) is a biological mediator which is synthesized from L-arginine by a family of nitric oxide synthases (NOS). Previously we have shown that NO is synthesized ex vivo by glomeruli obtained from animals with acute immune complex glomerulonephritis. We have now sought evidence for the in vivo induction of NOS in glomeruli by immunohistochemistry using specific antisera raised against a peptide sequence of inducible mouse macrophage NOS and by in situ hybridization. The expression of the enzyme was studied in kidneys of rats with acute unilateral immune complex glomerulonephritis, induced by cationized IgG, by immunohistochemistry. Inducible NOS (iNOS) was present in glomeruli in nephritic (left) kidneys at the time of maximum macrophage infiltration, both within intraglomerular mononuclear cells and cells emigrating into Bowman's space. iNOS expressing cells were also present in interstitial infiltrates. There was no expression in normal rat kidneys or in glomeruli in the non-nephritic (right) kidneys of experimental rats. In situ hybridization confirmed the immunohistochemical localization. These results provide the first direct evidence for the presence and localization of inducible NOS in glomeruli and support a significant role for NO in the pathogenesis of immune complex glomerulonephritis

New insights into the genetic etiology of Alzheimer's disease and related dementias

Characterization of the genetic landscape of Alzheimer's disease (AD) and related dementias (ADD) provides a unique opportunity for a better understanding of the associated pathophysiological processes. We performed a two-stage genome-wide association study totaling 111,326 clinically diagnosed/'proxy' AD cases and 677,663 controls. We found 75 risk loci, of which 42 were new at the time of analysis. Pathway enrichment analyses confirmed the involvement of amyloid/tau pathways and highlighted microglia implication. Gene prioritization in the new loci identified 31 genes that were suggestive of new genetically associated processes, including the tumor necrosis factor alpha pathway through the linear ubiquitin chain assembly complex. We also built a new genetic risk score associated with the risk of future AD/dementia or progression from mild cognitive impairment to AD/dementia. The improvement in prediction led to a 1.6- to 1.9-fold increase in AD risk from the lowest to the highest decile, in addition to effects of age and the APOE ε4 allele

The 'not-so-strange' body in the mirror: : A principal components analysis of direct and mirror self-observation

This document is the Accepted Manuscript version of the following article: Paul M. Jenkinson, and Catherine Preston, ‘The “not-so-strange” body in the mirror: A principal components analysis of direct and mirror self-observation’, Consciousness and Cognition, Vol. 48, pp. 262-272, first published online 4 January 2017, doi: http://dx.doi.org/10.1016/j.concog.2016.12.007 This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/.In this study we adopted a psychometric approach to examine how the body is subjectively experienced in a mirror. One hundred and twenty-four healthy participants viewed their body for five minutes directly or via a mirror, and then completed a 20-item questionnaire designed to capture subjective experiences of the body. PCA revealed a two-component structure for both direct and mirror conditions, comprising body evaluations (and alienation) and unusual feelings and perceptions. The relationship between these components and pre-existing tendencies for appearance anxiety, body dysmorphic-type beliefs, dissociative symptomatology, self-objectification and delusion ideation further supported the similarity between direct and mirror conditions; however, the occurrence of strange experiences like those reported to occur during prolonged face viewing was not confirmed. These results suggest that, despite obvious differences in visual feedback, observing the body via a mirror (as an outside observer) is subjectively equivalent to observing the body directly (from our own viewpoint).Peer reviewedFinal Accepted Versio

Analysis of the machinery and intermediates of the 5hmC-mediated DNA demethylation pathway in aging on samples from the MARKAGE Study

Gradual changes in the DNA methylation landscape occur throughout aging virtually in all human tissues. A widespread reduction of 5-methylcytosine (5mC), associated with highly reproducible site-specific hypermethylation, characterizes the genome in aging. Therefore, an equilibrium seems to exist between general and directional deregulating events concerning DNA methylation controllers, which may underpin the age-related epigenetic changes. In this context, 5mC-hydroxylases (TET enzymes) are new potential players. In fact, TETs catalyze the stepwise oxidation of 5mC to 5-hydroxymethylcytosine (5hmC), 5-formylcytosine (5fC) and 5-carboxylcytosine (5caC), driving the DNA demethylation process based on thymine DNA glycosylase (TDG)-mediated DNA repair pathway. The present paper reports the expression of DNA hydroxymethylation components, the levels of 5hmC and of its derivatives in peripheral blood mononuclear cells of age-stratified donors recruited in several European countries in the context of the EU Project ‘MARK-AGE’. The results provide evidence for an age-related decline of TET1, TET3 and TDG gene expression along with a decrease of 5hmC and an accumulation of 5caC. These associations were independent of confounding variables, including recruitment center, gender and leukocyte composition. The observed impairment of 5hmC-mediated DNA demethylation pathway in blood cells may lead to aberrant transcriptional programs in the elderly

New insights into the genetic etiology of Alzheimer’s disease and related dementias

Characterization of the genetic landscape of Alzheimer’s disease (AD) and related dementias (ADD) provides a unique opportunity for a better understanding of the associated pathophysiological processes. We performed a two-stage genome-wide association study totaling 111,326 clinically diagnosed/‘proxy’ AD cases and 677,663 controls. We found 75 risk loci, of which 42 were new at the time of analysis. Pathway enrichment analyses confirmed the involvement of amyloid/tau pathways and highlighted microglia implication. Gene prioritization in the new loci identified 31 genes that were suggestive of new genetically associated processes, including the tumor necrosis factor alpha pathway through the linear ubiquitin chain assembly complex. We also built a new genetic risk score associated with the risk of future AD/dementia or progression from mild cognitive impairment to AD/dementia. The improvement in prediction led to a 1.6- to 1.9-fold increase in AD risk from the lowest to the highest decile, in addition to effects of age and the APOE ε4 allele

Age-dependent expression of DNMT1 and DNMT3B in PBMCs from a large European population enrolled in the MARK-AGE study

Aging is associated with alterations in the content and patterns of DNA methylation virtually throughout the entire human lifespan. Reasons for these variations are not well understood. However, several lines of evidence suggest that the epigenetic instability in aging may be traced back to the alteration of the expression of DNA methyltransferases. Here, the association of the expression of DNA methyltransferases DNMT1 and DNMT3B with age has been analysed in the context of the MARK-AGE study, a large-scale cross-sectional study of the European general population. Using peripheral blood mononuclear cells, we assessed the variation of DNMT1 and DNMT3B gene expression in more than two thousand age-stratified women and men (35-75 years) recruited across eight European countries. Significant age-related changes were detected for both transcripts. The level of DNMT1 gradually dropped with aging but this was only observed up to the age of 64 years. By contrast, the expression of DNMT3B decreased linearly with increasing age and this association was particularly evident in females. We next attempted to trace the age-related changes of both transcripts to the influence of different variables that have an impact on changes of their expression in the population, including demographics, dietary and health habits, and clinical parameters. Our results indicate that age affects the expression of DNMT1 and DNMT3B as an almost independent variable in respect of all other variables evaluated

Improved Results in Supersymmetric Electroweak Baryogenesis

Electroweak baryogenesis provides a very attractive scenario to explain the origin of the baryon asymmetry. The mechanism of electroweak baryogenesis makes use of the baryon number anomaly and relies on physics that can be tested experimentally. It is today understood that, if the Higgs mass is not larger than 120 GeV, this mechanism may be effective within supersymmetric extensions of the Standard Model. In this work, we reconsider the question of baryon number generation at the electroweak phase transition within the context of the minimal supersymmetric extension of the Standard Model. We derive the relevant diffusion equations, give a consistent definition of the sources, and compare our results with those appearing in the recent literature on this subjectComment: 19 pages, 2 figure