135 research outputs found
Genetic variability of muscle biological characteristics of young Limousin bulls
Genetic parameters of 4 muscle biological characteristics (protein to DNA ratio (Pro/DNA), lactate dehydrogenase (LDH) activity, isocitrate dehydrogenase (ICDH) activity and the proportion of type I myosin heavy chains (MHC I)), in the Semitendinosus and the Longissimus thoracis, were estimated simultaneously with average daily gain (ADG), 480-d final weight (FW), carcass lean and fat contents (CL% and CF% respectively) in a sample of young Limousin bulls tested in station. The data came from 144 animals, the progeny of 15 sires. Sire and residual variances and covariances were estimated u using an expectation maximization restricted maximum likelihood (EM-REML) procedure applied to a multitrait mixed model. Heritability coefficients of production traits, ADG, FW, CL% and CF%, were 0.19, 0.49, 0.39 and 0.43, respectively, while heritability coefficients of muscle characteristics, Pro/DNA, LDH, ICDH and MHC I, were 0.11, 0.26, 1.03 and 0.35 respectively, in the Semitendinosus muscle and 0.29, 0.31, 0.28 and 0.41, respectively, in the Longissimus thoracis muscle. In both muscles, the oxidative activity of the ICDH appeared to be genetically associated with the proportion of type I myosin heavy chains and opposed to the glycolytic activity of the LDH. The LDH activity was clearly associated with higher muscle-to-fat ratio, while the opposite relationship was observed between that ratio and the ICDH activity or the MHC I proportion.Les paramètres génétiques de 4 caractéristiques biologiques - le rapport protéines /ADN (Pro/DNA), les activités de la lactate déshydrogénase (LDH) et de l’isocitrate déshydrogénase (ICDH) et la proportion en chaînes lourdes de myosine lente (MHC I) - des muscles Semitendinosus et Longissimus thoracis, et ceux du gain moyen quotidien (ADG), du poids vif finaL à 480 j (FW) et des teneurs de la carcasse en muscles et en dépôts adipeux (CL% and CF% respectivement), ont été estimés simultanément à partir d’un échantillon de taurillons Limousins contrôlés en station. Le fichier comprenait 144 veaux issus de 15 pères testés sur descendance. Les variances et covariances paternelles et résiduelles ont été estimées par la méthode du maximum de vraisemblance restreinte, avec l’algorithme d’espérance-maximisation, appliquée à un modèle mixte multicaractère (EM-REML). Les coefficients d’héritabilité des variables de production, ADG, FW, CL% et CF%, s’élevaient respectivement à 0,19, 0,49, 0,39 et 0,43, tandis que les coefficients d’héritabilité des caractéristiques musculaires, Pro/DNA, LDH, ICDH et MHC I, valaient respectivement 0,11, 0,26, 1,03 et 0,35 dans le muscle Semitendinosus et 0,29, 0,31, 0,28 et 0,41 dans le muscle Longissimus thoracis. Dans les 2 muscles, l’activité oxidative de l’ICDH était génétiquement associée à la proportion de myosine lente et opposée à l’activité glycolytique du LDH. Cette activité du LDH était positivement corrélée avec le rapport muscles / dépôts adipeux, alors qu’une relation inverse était observée avec l’activité de l’ICDH et la proportion de MHC I
Spillover Effects of Studying with Immigrant Students; A Quantile Regression Approach
Abstract: We analyze how the share of immigrant children in the classroom aects the educational attainment of native Dutch children in terms of their language and math performance at the end of primary school. Our paper studies the spill-over effects at different parts of the test score distribution of native Dutch students using a quantile regression approach. We fi nd no evidence of negative spillover effects of the classroom presence of immigrant children at the median of the test score distribution. In addition, there is no indication that these spill-over effects are present at other parts of the distribution.
Climate field reconstruction uncertainty arising from multivariate and nonlinear properties of predictors
Climate field reconstructions (CFRs) of the global annual surface air temperature (SAT) field and associated global area-weighted mean annual temperature (GMAT) are derived in a collection of pseudoproxy experiments for the past millennium. Pseudoproxies are modeled from temperature (T), precipitation (P), T + P, and VS-Lite (VSL), a nonlinear and multivariate proxy system model for tree ring widths. Spatial patterns of reconstruction skill and spectral bias for the T + P and VSL-derived CFRs are similar to those previously shown using temperature-only pseudoproxies but demonstrate overall degraded skill and spectral bias for SAT reconstruction. Analysis of GMAT spectra nevertheless suggests that the true GMAT frequency spectrum is resolved by those pseudoproxies (T, T + P, and VSL) that contain some temperature information. The results suggest that mixed temperature and moisture-responding paleoclimate data may produce actual GMAT reconstructions with skill, error, and spectral characteristics like those expected from univariate and linear temperature responders, but spatially resolved CFR results should be analyzed cautiousl
Replacement of Marine Fish Oil with de novo Omega-3 Oils from Transgenic Camelina sativa in Feeds for Gilthead Sea Bream (Sparus aurata L.)
Omega-3 (n-3) long-chain polyunsaturated fatty acids (LC-PUFA) are essential components of the diet of all vertebrates and. The major dietary source of n-3 LC-PUFA for humans has been fish and seafood but, paradoxically, farmed fish are also reliant on marine fisheries for fish meal and fish oil (FO), traditionally major ingredients of aquafeeds. Currently, the only sustainable alternatives to FO are vegetable oils, which are rich in C18 PUFA, but devoid of the eicosapentaenoic (EPA) and docosahexaenoic acids (DHA) abundant in FO. Two new n-3 LC-PUFA sources obtained from genetically modified (GM) Camelina sativa containing either EPA alone (ECO) or EPA and DHA (DCO) were compared to FO and wild-type camelina oil (WCO) in juvenile sea bream. Neither ECO nor DCO had any detrimental effects on fish performance, although final weight of ECO-fed fish (117 g) was slightly lower than that of FO- and DCO-fed fish (130 and 127 g, respectively). Inclusion of the GM-derived oils enhanced the n-3 LC-PUFA content in fish tissues compared to WCO, although limited biosynthesis was observed indicating accumulation of dietary fatty acids. The expression of genes involved in several lipid metabolic processes, as well as fish health and immune response, in both liver and anterior intestine were altered in fish fed the GM-derived oils. This showed a similar pattern to that observed in WCO-fed fish reflecting the hybrid fatty acid profile of the new oils. Overall the data indicated that the GM-derived oils could be suitable alternatives to dietary FO in sea bream
Effects of the total replacement of fish-based diet with plant-based diet on the hepatic transcriptome of two European sea bass (Dicentrarchus labrax) half-sibfamilies showing different growth rates with the plant-based diet
Background: Efforts towards utilisation of diets without fish meal (FM) or fish oil (FO) in finfish aquaculture have been being made for more than two decades. Metabolic responses to substitution of fishery products have been shown to impact growth performance and immune system of fish as well as their subsequent nutritional value, particularly in marine fish species, which exhibit low capacity for biosynthesis of long-chain poly-unsaturated fatty acids (LC-PUFA). The main objective of the present study was to analyse the effects of a plant-based diet on the hepatic transcriptome of European sea bass (Dicentrarchus labrax). Results: We report the first results obtained using a transcriptomic approach on the liver of two half-sibfamilies of the European sea bass that exhibit similar growth rates when fed a fish-based diet (FD), but significantly different growth rates when fed an all-plant diet (VD). Overall gene expression was analysed using oligo DNA microarrays (GPL9663). Statistical analysis identified 582 unique annotated genes differentially expressed between groups of fish fed the two diets, 199 genes regulated by genetic factors, and 72 genes that exhibited diet-family interactions. The expression of several genes involved in the LC-PUFA and cholesterol biosynthetic pathways was found to be up-regulated in fish fed VD, suggesting a stimulation of the lipogenic pathways. No significant diet-family interaction for the regulation of LC-PUFA biosynthesis pathways could be detected by microarray analysis. This result was in agreement with LC-PUFA profiles, which were found to be similar in the flesh of the two half-sibfamilies. In addition, the combination of our transcriptomic data with an analysis of plasmatic immune parameters revealed a stimulation of complement activity associated with an immunodeficiency in the fish fed VD, and different inflammatory status between the two half-sibfamilies. Biological processes related to protein catabolism, amino acid transaminations, RNA splicing and blood coagulation were also found to be regulated by diet, while the expression of genes involved in protein and ATP synthesis differed between the half-sibfamilies. Conclusions: Overall, the combined gene expression, compositional and biochemical studies demonstrated a large panel of metabolic and physiological effects induced by total substitution of both FM and FO in the diets of European sea bass and revealed physiological characteristics associated with the two half-sibfamilies
PaCTS 1.0: a crowdsourced reporting standard for paleoclimate data
The progress of science is tied to the standardization of measurements, instruments, and data. This is especially true in the Big Data age, where analyzing large data volumes critically hinges on the data being standardized. Accordingly, the lack of community-sanctioned data standards in paleoclimatology has largely precluded the benefits of Big Data advances in the field. Building upon recent efforts to standardize the format and terminology of paleoclimate data, this article describes the Paleoclimate Community reporTing Standard (PaCTS), a crowdsourced reporting standard for such data. PaCTS captures which information should be included when reporting paleoclimate data, with the goal of maximizing the reuse value of paleoclimate datasets, particularly for synthesis work and comparison to climate model simulations. Initiated by the LinkedEarth project, the process to elicit a reporting standard involved an international workshop in 2016, various forms of digital community engagement over the next few years, and grassroots working groups. Participants in this process identified important properties across paleoclimate archives, in addition to the reporting of uncertainties and chronologies; they also identified archive-specific properties and distinguished reporting standards for new vs. legacy datasets. This work shows that at least 135 respondents overwhelmingly support a drastic increase in the amount of metadata accompanying paleoclimate datasets. Since such goals are at odds with present practices, we discuss a transparent path towards implementing or revising these recommendations in the near future, using both bottom-up and top-down approaches
Decadal climate variability in the tropical Pacific: Characteristics, causes, predictability, and prospects
This is the author accepted manuscript. The final version is available from the American Association for the Advancement of Science via the DOI in this recordData and materials availability: All observational and model datasets used here are publicly available or available on request.Climate variability in the tropical Pacific affects global climate on a wide range of time scales. On interannual time scales, the tropical Pacific is home to the El Niño–Southern Oscillation (ENSO). Decadal variations and changes in the tropical Pacific, referred to here collectively as tropical Pacific decadal variability (TPDV), also profoundly affect the climate system. Here, we use TPDV to refer to any form of decadal climate variability or change that occurs in the atmosphere, the ocean, and over land within the tropical Pacific. “Decadal,” which we use in a broad sense to encompass multiyear through multidecadal time scales, includes variability about the mean state on decadal time scales, externally forced mean-state changes that unfold on decadal time scales, and decadal variations in the behavior of higher-frequency modes like ENSO
Performance, feed utilization, and hepatic metabolic response of weaned juvenile Atlantic bluefin tuna (Thunnus thynnus L.): effects of dietary lipid level and source
The development of formulated diets and feeds is essential to increase production of farmed tuna species. There is limited knowledge of this topic, mainly on Pacific Bluefin tuna (Thunnus orientalis) in Japan, whereas no major attempts have been made with Atlantic Bluefin tuna (Thunnus thynnus; ABT). In the present study, two trials were performed using inert formulated diets as on-growing feeds for weaned ABT juvenile in order to establish adequate dietary levels of both lipid and omega-3 long-chain polyunsaturated fatty acids (LC-PUFA). In a first trial, ABT (initial weight = 2.9±0.9g) were fed for 10 days with either a commercial (Magokoro®, MGK) or two experimental feeds with two different lipid levels (15 or 20%) using krill oil (KO) as the single lipid source in order to estimate the suitable lipid content. Fish fed MGK displayed the highest growth, followed by 15KO, with no differences in fish survival. Thus, a lipid content of 15% was considered better than 20% for ABT juveniles. In the second trial, fish (initial weight = 3.3 ± 0.6g) were fed either MGK, 15KO or a feed containing 15% lipid with a combination (1:1, v/v) KO and rapeseed oil (RO) (15KORO). Fish fed 15KO and 15KORO showed the highest growth in terms of weight and fork length (including weight gain and SGR). Increasing dietary lipid level or adding RO to the feeds did not increase liver lipid content. The liver fatty acid profile largely reflected dietary intake confirming very limited LC-PUFA biosynthetic activity for this teleost species. In this respect, liver of fish fed 15KO and 20KO displayed the highest contents of docosahexaenoic acid (DHA). The hepatic expression of genes of lipid and fatty acid metabolism, transcription factors, and antioxidant enzymes was investigated with many of the genes showing regulation by both dietary lipid and LC-PUFA contents. The present study showed promising results that suggested ABT juveniles can be on grown on inert dry feeds that supported good fish growth and the accumulation of the health-promoting fatty acid DHA. Further studies are required in order to fully elucidate lipid and fatty acid requirements of this iconic species regarding dietary sources and production costs.En prensa1,52
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