Introduction of a New Product in Ski Area

Tato diplomová práce se týká zavedení nového produktu v lyžařském areálu. Cílem této diplomové práce bylo prostřednictvím analýzy zjistit postoje a názory návštěvníků, zaměstnanců a oddílových členů Ski areálu Bílá na případné pořízení nové investice. Jako prostředek k získání potřebných informací k dosažení cíle této diplomové práce byla zvolena metoda online dotazování a hloubkového rozhovoru.This diploma thesis is focused on the Introduction of a New Product in Ski Area. The purpose of this thesis was to find out through analysis attitudes and opinions of visitors, employees and members of Bílá Ski Resort on the possible new investment. To obtain the goal of this diploma thesis was chosen the method of online questioning and deep in-depth interview.116 - Katedra marketingu a obchoduvelmi dobř

Involvement of nitric oxide (NO) and auxin in signal transduction of copper induced morphological responses in Arabidopsis seedlings

Background and Aims Plants are able to adapt to the environment dynamically through regulation of their growth and development. Excess copper (Cu2+ ), a toxic heavy metal, induces morphological alterations in plant organs; however, the underlying mechanisms are still unclear. With this in mind, the multiple signalling functions of nitric oxide (NO) in plant cells and its possible regulatory role and relationship with auxin were examined during Cu2+ -induced morphological responses. Methods Endogenous auxin distribution was determined by microscopic observation of X-Gluc-stained DR5::GUS arabidopsis, and the levels of NO, superoxide and peroxynitrite were detected by ¿uorescence microscopy. As well as wild-type, NO-overproducer (nox1) and -de¿cient (nia1nia2 and nia1nia2noa1-2) arabidopsis plants were used. Key Results Cu2+ at a concentration of 50mM resulted in a large reduction in cotyledon area and hypocotyl and primary root lengths, accompanied by an increase in auxin levels. In cotyledons, a low Cu2+ concentration promoted NO accumulation, which was arrested by nitric oxide synthase or nitrate reductase inhibitors. The 5-mM Cu2+ -induced NO synthesis was not detectable in nia1nia2 or nia1nia2noa1-2 plants. In roots, Cu2+ caused a decrease of the NO level which was not associated with superoxide and peroxynitrite formation. Inhibition of auxin transport resulted in an increase in NO levels, while exogenous application of an NO donor reduced DR5::GUS expression. The elongation processes of nox1 were not sensitive to Cu2+ , but NO-de¿cient plants showed diverse growth responses. ConclusionsIn plant organs, Cu2+ excess results in severe morphological responses during which the endogenous hormonal balance and signal transduction are affected. Auxin and NO negatively regulate each other¿s level and NO intensi¿es the metal-induced cotyledon expansion, but mitigates elongation processes under Cu2+ exposurePetó, A.; Lehotai, N.; Lozano Juste, J.; Leon Ramos, J.; Tari, I.; Erdei, L.; Kolbert, Z. (2011). Involvement of nitric oxide (NO) and auxin in signal transduction of copper induced morphological responses in Arabidopsis seedlings. Annals of Botany. 108(3):449-457. doi:10.1093/aob/mcr176S449457108

Collaborative European NeuroTrauma Effectiveness Research in Traumatic Brain Injury (CENTER-TBI): A Prospective Longitudinal Observational Study

BACKGROUND: Current classification of traumatic brain injury (TBI) is suboptimal, and management is based on weak evidence, with little attempt to personalize treatment. A need exists for new precision medicine and stratified management approaches that incorporate emerging technologies. OBJECTIVE: To improve characterization and classification of TBI and to identify best clinical care, using comparative effectiveness research approaches. METHODS: This multicenter, longitudinal, prospective, observational study in 22 countries across Europe and Israel will collect detailed data from 5400 consenting patients, presenting within 24 hours of injury, with a clinical diagnosis of TBI and an indication for computed tomography. Broader registry-level data collection in approximately 20 000 patients will assess generalizability. Cross sectional comprehensive outcome assessments, including quality of life and neuropsychological testing, will be performed at 6 months. Longitudinal assessments will continue up to 24 months post TBI in patient subsets. Advanced neuroimaging and genomic and biomarker data will be used to improve characterization, and analyses will include neuroinformatics approaches to address variations in process and clinical care. Results will be integrated with living systematic reviews in a process of knowledge transfer. The study initiation was from October to December 2014, and the recruitment period was for 18 to 24 months. EXPECTED OUTCOMES: Collaborative European NeuroTrauma Effectiveness Research in TBI should provide novel multidimensional approaches to TBI characterization and classification, evidence to support treatment recommendations, and benchmarks for quality of care. Data and sample repositories will ensure opportunities for legacy research. DISCUSSION: Comparative effectiveness research provides an alternative to reductionistic clinical trials in restricted patient populations by exploiting differences in biology, care, and outcome to support optimal personalized patient management

SLAVKOV - REVITALISATION OF BONAPARTE CENTER

This bachelor thesis deals with the revitalisation of community centre Bonaparte in Slavkov u Brna. Architectural design of the reconstruction of historical building “Záložna“ and new building in the gap in between houses brings new attractive functions into the historical city core. It aims for renewal of the values of historical object and at the same time tries to achieve the contemporary architectural expression in material solution, respecting the scale of historical environment

Annen Gate

A multifunctional city block is designed for the currently empty place in Brno, on the corner of Pekarská and Anenská streets. The urban, architectural and material-spatial solution tries to respect the scale and cultural-historical context of the place. The proposed main function of housing is enlivened by additional functions of administration and trade and services on the ground floor. The corner is an important compositional point and the proposed round and glazed shape of the building will form a visual dominant on the view from Pekarská Street. In the city block, the inner space of the courtyard is created, in which the historical heritage of the retaining wall of the hill on which the church once stood is presented. The passage through the city block is possible in the route of the former street U sv. Anny. The lack of green spaces in the courtyard is compensated by the creation of green roofs for residents and users. The architectural expression of the townhouse tries to follow the traditions of modernism, typical for the city of Brno

Optimization of purification of recombinant nitrilase from Arthroderma benhamie

Department of BiochemistryKatedra biochemieFaculty of SciencePřírodovědecká fakult

SLAVKOV - REVITALISATION OF BONAPARTE CENTER

Práca sa zaoberá revitalizáciou spoločenského centra Bonaparte v Slavkove u Brna. Architektonický návrh rekonštrukcie historického objektu Záložny i novostavby v preluke prináša nové atraktívne funkcie do historického jadra mesta. Zameriava sa na obnovenie hodnôt historického objektu a súčasne sa snaží o novodobý architektonický výraz v materiálovom riešení objektu novostavby v preluke, rešpektujúc mierku historického prostredia.This bachelor thesis deals with the revitalisation of community centre Bonaparte in Slavkov u Brna. Architectural design of the reconstruction of historical building “Záložna“ and new building in the gap in between houses brings new attractive functions into the historical city core. It aims for renewal of the values of historical object and at the same time tries to achieve the contemporary architectural expression in material solution, respecting the scale of historical environment.