Molusco contagioso: sorologia e microscopia eletrônica em vinte e um pacientes

Twenty one cases of molluscum contagiosum virus disease were collected for electron microscopical and serological tests. Molluscum virus was detected in the crust, inside the vacuoles formed in the keratinocytes cells. The patients developed specific antibodies to the virus detected by complement fixation test.Vinte e um casos de molusco contagioso foram escolhidos para serem analisados por testes sorológicos e de microscopia eletrônica. O vírus do molusco foi detectado nas crostas e observados também nos vacúolos formados dentro dos queratinócitos. Os pacientes desenvolveram anticorpos fixadores do complemento para o vírus

Influência do número de camadas na propriedade mecânica de espécimes fabricados com atadura gessada usados para confeccionar splints ortopédicos

OBJECTIVE: To evaluate the effects of varying numbers of layers of plaster of Paris bandages on the mechanical properties of specimens used on the construction of orthopedic splints. METHODS: Rectangular plate-shaped and cylinder-shaped specimens were constructed and assigned to two groups simulating plaster slabs and cast and further divided into six subgroups according to the number of layers used: 3, 6, 8, 10, 12 and 14 layers. The specimens were subjected to either a three-point bending test (plates/slab) or compressive strength test (cylinders/cast). The following mechanical properties were evaluated: maximum load, elastic limit load and stiffness. Specimen weight was also calculated. Data was analyzed using Kruskal-Wallis and the least significant difference (LSD) tests. RESULTS: Pairwise comparisons of the subgroups 10x12 and 10x14 revealed significant differences for all mechanical properties (p<0.05). The results of this study suggest that when the goal is to construct appliances with high mechanical strength, regardless of weight, such as serial plaster slabs splints for stimulating tissue growth through the application of gradual load, splints made with plaster of Paris bandages with 12 or 14 layers should be preferred. For orthotic devices such as positioning orthotics, the use of 10 layers plaster bandages slab splints is advisable as they were found to have better correlation between mechanical strength and weight in comparison to those made wtih 6 or 8 layers. CONCLUSION: Based on the findings of this study, we suggest the use of 10 layers of plaster of Paris for the construction of orthopedic splints.OBJETIVO: Avaliar as propriedades mecânicas de amostras fabricadas a partir de ataduras de gesso que são utilizadas em órteses ortopédicas e que variam quanto ao número de camadas. MÉTODOS: Foram confeccionados espécimes em forma de placa retangular e em forma cilíndrica, divididos em dois grupos que simulavam splint e gesso circular, os quais foram divididos em seis subgrupos de acordo com o número de camadas utilizadas, ou seja, três, seis, oito, dez, 12 e 14 camadas. Os espécimes foram submetidos a um teste de inclinação de três pontos (placas/splint) ou teste de resistência à compressão (cilindros/gesso circular). As seguintes propriedades mecânicas foram avaliadas: carga máxima e carga no limite de elasticidade e rigidez. O peso da amostra foi calculado. Os dados foram analisados estatisticamente pelos testes de Kruskal-Wallis e diferença mínima significativa (DMS). Comparações pareadas entre os subgrupos 10x12 e 10x14 revelaram diferenças significativas para todas as propriedades mecânicas (p<0,05). RESULTADOS: Os resultados sugerem que, quando o objetivo é construir aparelhos com alta resistência mecânica, independente do peso, tais como órteses seriadas de posicionamento para simular força gradual aplicada no tecido para a melhoria da amplitude de movimento, talas de 12 ou 14 camadas devem ser preferidas. Para os aparelhos ortopédicos que irão ser submetidos a esforços de baixa intensidade, aconselha-se a utilização de dez camadas para as órteses, porque houve uma melhor correlação entre a resistência mecânica e peso para as amostras fabricadas com dez camadas de atadura gessada comparadas com aquelas confeccionadas com seis ou oito camadas. CONCLUSÂO: Baseado nos achados deste estudo, sugere-se a utilização de dez camadas na confecção de órteses ortopédicas

Bolometric luminosity variations in the Luminous Blue Variable AFGL2298

We characterise the variability in the physical properties of the luminous blue variable AFGL2298 between 1989-2008. In conjunction with published data from 1989-2001, we have undertaken a long term (2001-2008) near-IR spectroscopic and photometric observational campaign for this star and utilise a non-LTE model atmosphere code to interpret these data. We find AFGL2298 to have been highly variable during the two decades covered by the observational datasets. Photometric variations of >1.6 mag have been observed in the JHK wavebands; however, these are not accompanied by correlated changes in near-IR colour. Non-LTE model atmosphere analysis of 4 epochs of K band spectroscopy obtained between 2001-7 suggests that the photometric changes were driven by expansion and contraction of the stellar photosphere accompanied by comparatively small changes in the stellar temperature. Unclumped mass loss rates throughout this period were modest and directly comparable to those of other highly luminous LBVs. However, the bolometric luminosity of AFGL2298 appears to have varied by at least a factor of ~2 between 1989-2008, with it being one of the most luminous stars in the Galaxy during maximum. Comparison to other LBVs that have undergone non bolometric luminosity conserving `eruptions' shows such events to be heterogeneous, with AFGL2298 the least extreme example. These results - and the diverse nature of both the quiescent LBVs and associated ejecta - may offer support to the suggestion that more than one physical mechanism is responsible for such behaviour. [ABRIDGED]Comment: 12 pages, 6 figures, accepted for publication in Astronomy and Astrophysic

Genome of the Avirulent Human-Infective Trypanosome—Trypanosoma rangeli

Background: Trypanosoma rangeli is a hemoflagellate protozoan parasite infecting humans and other wild and domestic mammals across Central and South America. It does not cause human disease, but it can be mistaken for the etiologic agent of Chagas disease, Trypanosoma cruzi. We have sequenced the T. rangeli genome to provide new tools for elucidating the distinct and intriguing biology of this species and the key pathways related to interaction with its arthropod and mammalian hosts.  Methodology/Principal Findings: The T. rangeli haploid genome is ,24 Mb in length, and is the smallest and least repetitive trypanosomatid genome sequenced thus far. This parasite genome has shorter subtelomeric sequences compared to those of T. cruzi and T. brucei; displays intraspecific karyotype variability and lacks minichromosomes. Of the predicted 7,613 protein coding sequences, functional annotations could be determined for 2,415, while 5,043 are hypothetical proteins, some with evidence of protein expression. 7,101 genes (93%) are shared with other trypanosomatids that infect humans. An ortholog of the dcl2 gene involved in the T. brucei RNAi pathway was found in T. rangeli, but the RNAi machinery is non-functional since the other genes in this pathway are pseudogenized. T. rangeli is highly susceptible to oxidative stress, a phenotype that may be explained by a smaller number of anti-oxidant defense enzymes and heatshock proteins.  Conclusions/Significance: Phylogenetic comparison of nuclear and mitochondrial genes indicates that T. rangeli and T. cruzi are equidistant from T. brucei. In addition to revealing new aspects of trypanosome co-evolution within the vertebrate and invertebrate hosts, comparative genomic analysis with pathogenic trypanosomatids provides valuable new information that can be further explored with the aim of developing better diagnostic tools and/or therapeutic targets

The evolution and masses of the neutron star and donor star in the high mass X-ray binary OAO 1657-415

We report near-infrared radial velocity measurements of the recently identified donor star in the high mass X-ray binary system OAO 1657-415 obtained in the H band using ISAAC on the VLT. Cross-correlation methods were employed to construct a radial velocity curve with a semi-amplitude of $22.1 \pm 3.5$ km s$^{-1}$. Combined with other measured parameters of this system this provides a dynamically determined neutron star mass of $1.42 \pm 0.26 M$_{\odot}$and a mass of$14.3 \pm 0.8 M$_{\odot}$ for the Ofpe/WN9 highly evolved donor star. OAO 1657-415 is an eclipsing High Mass X-ray binary pulsar with the largest eccentricity and orbital period of any within its class. Of the ten known eclipsing X-ray binary pulsars OAO 1657-415 becomes the ninth with a dynamically determined neutron star mass solution and only the second in an eccentric system. Furthermore, the donor star in OAO 1657-415 is much more highly evolved than the majority of the supergiant donors in other High Mass X-ray binaries (HMXBs), joining a small but growing list of HMXBs donors with extensive hydrogen depleted atmospheres. Considering the evolutionary development of OAO 1657-415, we have estimated the binding energy of the envelope of the mass donor and find that there is insufficient energy for the removal of the donor's envelope via spiral-in, ruling out a Common Envelope evolutionary scenario. With its non-zero eccentricity and relatively large orbital period the identification of a definitive evolutionary pathway for OAO1657-415 remains problematic, we conclude by proposing two scenarios which may account for OAO1657-415 current orbital configuration.Comment: 9 pages, 4 figures. Accepted for publication in MNRA

The weak magnetic field of the O9.7 supergiant zeta Orionis A

We report here the detection of a weak magnetic field of 50 - 100 G on the O9.7 supergiant zeta Ori A, using spectropolarimetric observations obtained with NARVAL at the 2m Telescope Bernard Lyot atop Pic du Midi (France). zeta Ori A is the third O star known to host a magnetic field (along with theta^1 Ori C and HD 191612), and the first detection on a 'normal' rapidly-rotating O star. The magnetic field of zeta Ori A is the weakest magnetic field ever detected on a massive star. The measured field is lower than the thermal equipartition limit (about 100 G). By fitting NLTE model atmospheres to our spectra, we determined that zeta Ori A is a 40 Msun star with a radius of 25 Rsun and an age of about 5 - 6 Myr, showing no surface nitrogen enhancement and losing mass at a rate of about 2x10^(-6) Msol/yr. The magnetic topology of zeta Ori A is apparently more complex than a dipole and involves two main magnetic polarities located on both sides of the same hemisphere; our data also suggest that zeta Ori A rotates in about 7.0 d and is about 40 degrees away from pole-on to an Earth-based observer. Despite its weakness, the detected magnetic field significantly affects the wind structure; the corresponding Alfven radius is however very close to the surface, thus generating a different rotational modulation in wind lines than that reported on the two other known magnetic O stars. The rapid rotation of zeta Ori A with respect to theta^1 Ori C appears as a surprise, both stars having similar unsigned magnetic fluxes (once rescaled to the same radius); it may suggest that the sub-equipartition field detected on zeta Ori A is not a fossil remnant (as opposed to that of theta^1 Ori C and HD 191612), but the result of an exotic dynamo action produced through MHD instabilities.Comment: 12 page