Serum Levels of Advanced Glycation End Products Are Associated with In-Stent Restenosis in Diabetic Patients

The formation of advanced glycation end products (AGEs), in various tissues has been known to enhance immunoinflammatory reactions and local oxidant stresses in long standing diabetes. Recently, AGEs have been reported to play a role in neointimal formation in animal models of arterial injury. We attempted to determine whether the serum levels of AGEs are associated with coronary restenosis in diabetic patients. Blood samples were collected from diabetic patients with coronary artery disease undergoing stent implantation and the serum levels of AGEs were analyzed by the fluorescent intensity method. The development of in-stent restenosis (ISR) was evaluated by a 6-month follow-up coronary angiography. A total of 263 target lesions were evaluated, in 203 patients. The ISR rate in the high-AGE (>170 U/ml) group (40.1%) was significantly higher than in the low-AGE group (≤170 U/ml) (19.6%) (p<0.001). Furthermore, multivariate analysis revealed that a high level of serum AGEs is an independent risk factor for the development of ISR (odds ratio, 2.659; 95% CI, 1.431-4.940; p=0.002). The serum levels of AGEs constitute an excellent predictive factor for ISR, and should be one of the guidelines for medical therapy and interventional strategy to prevent ISR in diabetic patients

The Aetiology of Diabetic Neuropathy: the Combined Roles of Metabolic and Vascular Defects

Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/75362/1/j.1464-5491.1995.tb00544.x.pd

Early- and advanced non-enzymatic glycation in diabetic vascular complications: the search for therapeutics

Cardiovascular disease is a common complication of diabetes and the leading cause of death among people with diabetes. Because of the huge premature morbidity and mortality associated with diabetes, prevention of vascular complications is a key issue. Although the exact mechanism by which vascular damage occurs in diabetes in not fully understood, numerous studies support the hypothesis of a causal relationship of non-enzymatic glycation with vascular complications. In this review, data which point to an important role of Amadori-modified glycated proteins and advanced glycation endproducts in vascular disease are surveyed. Because of the potential role of early- and advanced non-enzymatic glycation in vascular complications, we also described recent developments of pharmacological inhibitors that inhibit the formation of these glycated products or the biological consequences of glycation and thereby retard the development of vascular complications in diabetes

Regulation of expression of the p21CIP1 gene by the transcription factor ZNF217 and MDM2

Using mouse double minute 2 (MDM2) protein-specific affinity chromatography and mass spectrometry, we have isolated the protein product of the oncogene znf217, which is a transcription factor and a component of a Hela-S-derived HDAC1 complex, as a novel MDM2-interacting protein. When co-expressed in cultured cancer cells, ZNF217 forms a complex with MDM2 and its ectopic over-expression reduces the steady-state levels of acetylated p53 in cell lines, suppressing its ability to activate the expression of a p21 promoter construct. In-silico analysis of the p21 promoter revealed the presence of several ZNF217-binding sites. These findings suggest that MDM2 controls p21 expression by at least 2 mechanisms: through ZNF217-mediated recruitment of HDAC1/MDM2 activity, which inhibits p53 acetylation; and through direct interaction with its binding site(s) on the p21 promoter. © 2016 Published by NRC Research Press

Increased Δ133p53 mRNA in lung carcinoma corresponds with reduction of p21 expression

La “intervención social” no es sinónimo de Trabajo Social y tampoco refiere exclusivamente a “procedimientos”. En el campo académico resulta complejo reconocer a la “intervención social” como campo de conocimientos al igual que los conocimientos políticos o sociológicos, pero nos proponemos fundamentar en este texto que la Intervención Social sí constituye una categoría teórica en cuya definición ha aportado y aporta el Trabajo Social, pero también la Ciencia Política y la Sociología. En términos generales, podemos decir que la intervención social es un concepto que abarca el conjunto de procesos y estrategias que tienen lugar en la implementación-gestión de políticas sociales y en las múltiples formas de acción colectiva que desarrollan los sujetos en torno al acceso a derechos. Así planteado, estamos reconociendo un campo de conocimientos que incluye al Estado como garante de derechos y regulador en los procesos de redistribución democrática de los recursos en las sociedades desiguales en las que vivimos, al igual que a la sociedad movilizada en torno a demandas, necesidades y reivindicaciones en la conquista de esos derechos, en la reformulación de políticas y en los modos de acceso - o no acceso - a los mismos.“Social intervention” is not synonymous with Social Work and does not refer exclusively to “procedures.” In the academic field it is complex to recognize “social intervention” as a field of knowledge as well as political or sociological knowledge, but we propose to base in this text that Social Intervention does constitute a theoretical category in whose definition it has contributed and provides the Social Work, but also Political Science and Sociology. In general terms, we can say that social intervention is a concept that encompasses the set of processes and strategies that take place in the implementation-management of social policies and in the multiple forms of collective action that subjects develop around access to rights . Thus raised, we are recognizing a field of knowledge that includes the State as guarantor of rights and regulator in the processes of democratic redistribution of resources in the unequal societies in which we live, as well as to the society mobilized around demands, needs and claims in the conquest of those rights, in the reformulation of policies and in the modes of access - or non-access - to them.Facultad de Trabajo Socia

Accumulation of carbapenem resistance mechanisms in VIM-2-producing Pseudomonas aeruginosa under selective pressure

Pseudomonas aeruginosa has the potential to achieve resistance to carbapenems via the acquisition of carbapenemase-encoding genes, the downregulation of the OprD porin, the overexpression of efflux systems and the overproduction of cephalosporinases. One hundred and fifty carbapenem-non-susceptible isolates from 2008 to 2010 were screened for carbapenemase production, OprD porin loss, efflux pumps overexpression and inducible AmpC beta-lactamase production. For comparison reasons, the presence of the same mechanisms was also assessed in a previous collection of 30 carbapenem-non-susceptible P. aeruginosa isolated between 2003 and 2005. Results showed the accumulation of various resistance mechanisms among VIM-2 producers isolated between 2008 and 2010 with a parallel considerable increase in imipenem MIC90 and the geometric mean of the MIC values of imipenem and meropenem between the two study groups. The accumulation of carbapenem resistance mechanisms highlights the potential of this formidable pathogen for evolutionary success under antibiotic selective pressure