GR 290 (Romano's Star): 2. Light history and evolutionary state

We have built the historical light curve of the luminous variable GR 290 back to 1901, from old observations of the star found in several archival plates of M 33. These old recordings together with published and new data show that for at least half a century the star was in a low luminosity state, with B ~18. After 1960, five large variability cycles of visual luminosity were recorded. The amplitude of the oscillations was seen increasing towards the 1992-1994 maximum, then decreasing during the last maxima. The recent light curve indicates that the photometric variations have been quite similar in all the bands, and that the B-V color index has been constant within +/-0.1 m despite the 1.5m change of the visual luminosity. The spectrum of GR 290 at the large maximum of 1992-94, was equivalent to late-B type, while, during 2002-2014, it has varied between WN10h-11h near the visual maxima to WN8h-9h at the luminosity minima. We have detected, during this same period, a clear anti-correlation between the visual luminosity, the strength of the HeII 4686 A emission line, the strength of the 4600-4700 A lines blend and the spectral type. From a model analysis of the spectra collected during the whole 2002-2014 period we find that the Rosseland radius R_{2/3}, changed between the minimum and maximum luminosity phases by a factor of 3, while T_eff varied between about 33,000 K and 23,000 K. The bolometric luminosity of the star was not constant, but increased by a factor of ~1.5 between minimum and maximum luminosity, in phase with the apparent luminosity variations. In the light of current evolutionary models of very massive stars, we find that GR 290 has evolved from a ~60 M_Sun progenitor star and should have an age of about 4 million years. We argue that it has left the LBV stage and is moving to a Wolf-Rayet stage of late nitrogen spectral type.Comment: Accepted on The Astronomical Journal, 10 figures. Replaced because the previous uploaded file was that without the final small corrections requested by the refere

An HST/WFPC2 survey of bright young clusters in M31. I. VdB0, a massive star cluster seen at t ≃ 25 Myr

Aims. We introduce our imaging survey of possible young massive globular clusters in M31 performed with the Wide Field and Planetary Camera 2 (WFPC2) on the Hubble Space Telescope (HST). We obtained shallow (to B ~ 25) photometry of individual stars in 20 candidate clusters. We present here details of the data reduction pipeline that is being applied to all the survey data and describe its application to the brightest among our targets, van den Bergh 0 (VdB0), taken as a test case. Methods. Point spread function fitting photometry of individual stars was obtained for all the WFPC2 images of VdB0 and the completeness of the final samples was estimated using an extensive set of artificial stars experiments. The reddening, the age and the metallicity of the cluster were estimated by comparing the observed color magnitude diagram (CMD) with theoretical isochrones. Structural parameters were obtained from model-fitting to the intensity profiles measured within circular apertures on the WFPC2 images. Results. Under the most conservative assumptions, the stellar mass of VdB0 is M> 2.4 x 10^4 M_☉ , but our best estimates lie in the range ≃4-9 x 10^4 M_☉. The CMD of VdB0 is best reproduced by models having solar metallicity and age ≃25 Myr. Ages less than ≃12 Myr and greater than ≃60 Myr are clearly ruled out by the available data. The cluster has a remarkable number of red super giants (≳18) and a CMD very similar to Large Magellanic Cloud clusters usually classified as young globulars such as NGC 1850, for example. Conclusions. VdB0 is significantly brighter (≳1 mag) than Galactic open clusters of similar age. Its present-day mass and half-light radius ((r_h = 7.4 pc) are more typical of faint globular clusters than of open clusters. However, given its position within the disk of M31, it is expected to be destroyed by dynamical effects, in particular by encounters with giant molecular clouds, within the next ~4 Gyr

The Gaia spectrophotometric standard stars survey -II. Instrumental effects of six ground-based observing campaigns

The Gaia SpectroPhotometric Standard Stars (SPSS) survey started in 2006, it was awarded almost 450 observing nights, and accumulated almost 100,000 raw data frames, with both photometric and spectroscopic observations. Such large observational effort requires careful, homogeneous, and automated data reduction and quality control procedures. In this paper, we quantitatively evaluate instrumental effects that might have a significant (i.e.,$\geq$1%) impact on the Gaia SPSS flux calibration. The measurements involve six different instruments, monitored over the eight years of observations dedicated to the Gaia flux standards campaigns: DOLORES@TNG in La Palma, EFOSC2@NTT and ROSS@REM in La Silla, [email protected] in Calar Alto, BFOSC@Cassini in Loiano, and [email protected] in San Pedro Martir. We examine and quantitatively evaluate the following effects: CCD linearity and shutter times, calibration frames stability, lamp flexures, second order contamination, light polarization, and fringing. We present methods to correct for the relevant effects, which can be applied to a wide range of observational projects at similar instruments.Comment: 16 pages, 13 figures, accepted for publication in Astron. Nach

Age as the Second Parameter in NGC 288 / NGC 362? I. Turnoff Ages: a Purely Differential Comparison

We present deep V,I photometry of the globular clusters NGC 288, NGC 362 and NGC 1851 obtained during a single observational run under strictly homogeneous conditions. We use the bimodal horizontal branch (HB) of NGC 1851 as a ``bridge'' to obtain the optimum relative match between the HBs of NGC 288 and NGC 362. In this way we can effectively remove the uncertainties associated with distance, reddening and inhomogeneities in the absolute calibration, thus obtaining a very robust, purely differential estimate of the age difference between these two clusters. According to the bridge test, NGC 288 is found to be older than NGC 362 by 2 +- 1 Gyr. This result is fully confirmed also by all classical differential age diagnostics, either based on the luminosity or color of the main sequence turnoff point.Comment: Accepted by The Astronomical Journal - 30 pages, 6 tables, 14 figures, LaTeX, the emulateapj5.sty macro is used. Fig. 2 and Fig. 3 are provided in reduced resolution. Full resolution versions available upon request to the first author ([email protected]). Minor changes due to modifications in the companion paper (Pap II

The first two transient supersoft X-ray sources in M 31 globular clusters and the connection to classical novae

Classical novae (CNe) have been found to represent the major class of supersoft X-ray sources (SSS) in our neighbour galaxy M 31. We determine properties and evolution of the two first SSSs ever discovered in the M 31 globular cluster (GC) system. We have used XMM-Newton, Chandra and Swift observations of the centre region of M 31 to discover both SSS and to determine their X-ray light curves and spectra. We performed detailed analysis of XMM-Newton EPIC PN spectra of the source in Bol 111 (SS1) using blackbody and NLTE white dwarf (WD) atmosphere models. For the SSS in Bol 194 (SS2) we used optical monitoring data to search for an optical counterpart. Both GC X-ray sources were classified as SSS. We identify SS1 with the CN M31N 2007-06b recently discovered in the M 31 GC Bol 111. For SS2 we did not find evidence for a recent nova outburst and can only provide useful constraints on the time of the outburst of a hypothetical nova. The only known CN in a M 31 GC can be identified with the first SSS found in a M31 GC. We discuss the impact of our observations on the nova rate for the M 31 GC system.Comment: 11 pages, 9 figures, 4 tables, submitted to A&A; v2: accepted versio

Gaia data release 1: Principles of the photometric calibration of the G band

Context. Gaia is an ESA cornerstone mission launched on 19 December 2013 aiming to obtain the most complete and precise 3D map of our Galaxy by observing more than one billion sources. This paper is part of a series of documents explaining the data processing and its results for Gaia Data Release 1, focussing on the G band photometry. Aims. This paper describes the calibration model of the Gaia photometric passband for Gaia Data Release 1. Methods. The overall principle of splitting the process into internal and external calibrations is outlined. In the internal calibration, a self-consistent photometric system is generated. Then, the external calibration provides the link to the absolute photometric flux scales. Results. The Gaia photometric calibration pipeline explained here was applied to the first data release with good results. Details are given of the various calibration elements including the mathematical formulation of the models used and of the extraction and preparation of the required input parameters (e.g. colour terms). The external calibration in this first release provides the absolute zero point and photometric transformations from the Gaia G passband to other common photometric systems. Conclusions. This paper describes the photometric calibration implemented for the first Gaia data release and the instrumental effects taken into account. For this first release no aperture losses, radiation damage, and other second-order effects have not yet been implemented in the calibration.Comment: 15 pages, 16 figures, Gaia data release 1 documentation special volum

Time variability of X-ray sources in the M 31 centre field

We present an extension to our XMM-Newton X-ray source catalogue of M 31, containing 39 newly found sources. In order to classify and identify more of the sources we search for X-ray time variability in XMM-Newton archival data of the M 31 centre field. As a source list we used our extended catalogue based on observations covering the time span from June 2000 to July 2004. We then determined the flux or at least an upper limit at the source positions for each observation. Deriving the flux ratios for the different observations and searching for the maximum flux difference we determined variability factors. We also calculated the significance of the flux ratios. Using hardness ratios, X-ray variability and cross correlations with catalogues in the X-ray, optical, infrared and radio regimes, we detected three super soft source candidates, one supernova remnant and six supernova remnant candidates, one globular cluster candidate, three X-ray binaries and four X-ray binary candidates. Additionally we identified one foreground star candidate and classified fifteen sources with hard spectra, which may either be X-ray binaries or Crab-like supernova remnants in M 31 or background active galactic nuclei. The remaining five sources stay unidentified or without classification. Based on the time variability results we suggest six sources, which were formerly classified as "hard", to be X-ray binary candidates. The classification of one other source (XMMM31 J004236.7+411349) as a supernova remnant, has to be rejected due to the distinct time variability we found. We now classify this source as a foreground star.Comment: 13 pages, 6 figures, accepted for publication in A&

The deep XMM-Newton Survey of M 31

Aims. M 31 has been completely imaged for the first time obtaining a luminosity lower limit ~10^35 erg s-1 in the 0.2-4.5 keV band. Our XMM-Newton EPIC survey combines archival observations with observations taken between June 2006 and February 2008. The main goal of the paper is a study of the X-ray source population of M 31. Methods. An X-ray catalogue of 1897 sources was created; 914 were detected for the first time. Source classification and identification were based on X-ray hardness ratios, spatial extent of the sources, and by cross correlating with catalogues in the X-ray, optical, infrared and radio wavelengths. We also analysed the long-term variability of the X-ray sources. Results. Fourteen of the 30 supersoft source candidates represent supersoft emission of optical novae. Many of the 25 supernova remnants (SNRs) and 31 SNR candidates lie within the 10 kpc dust ring and other star forming regions in M 31; this connection between SNRs and star forming regions implies that most of the remnants originate in type II supernovae. The brightest sources in X-rays in M 31 belong to the class of X-ray binaries (XRBs). Ten low mass XRBs (LMXBs) and 26 LMXB candidates were identified based on their temporal variability. In addition 36 LMXBs and 17 LMXB candidates were identified due to correlations with globular clusters and globular cluster candidates. From optical and X-ray colour-colour diagrams, possible high mass XRB (HMXB) candidates were selected. Two of these candidates have an X-ray spectrum as is expected for an HMXB containing a neutron star primary. Conclusions. While our survey has greatly improved our understanding of the X-ray source populations in M 31, at this point 65% of the sources can still only be classified as "hard" sources. Deeper observations in X-ray and at other wavelengths would help classify these sources. ABRIDGEDComment: 51 pages, 36 figures, submitted to A&

A HST/WFPC2 survey of bright young clusters in M31. I. VdB0, a massive star cluster seen at ~= 25 Myr

{Aims.} We introduce our imaging survey of possible young massive globular clusters in M31 performed with the Wide Field and Planetary Camera 2 (WFPC2) on the Hubble Space Telescope (HST). We present here details of the data reduction pipeline that is being applied to all the survey data and describe its application to the brightest among our targets, van den Bergh 0 (VdB0), taken as a test case. {Methods.} The reddening, the age and the metallicity of the cluster are estimated by comparison of the observed Color Magnitude Diagram (CMD) with theoretical isochrones. {Results.} Under the most conservative assumptions the stellar mass of VdB0 is M > 2.4 x 10^4 M_sun, but our best estimates lie in the range ~ 4-9 x 10^4 M_sun. The CMD of VdB0 is best reproduced by models having solar metallicity and age = 25 Myr. Ages smaller than = 12 Myr and larger than = 60 Myr are clearly ruled out by the available data. The cluster has a remarkable number of Red Super Giants (> 18) and a CMD very similar to Large Magellanic Cloud clusters usually classified as young globulars such as NGC 1850, for example. {Conclusions.} VdB0 is significantly brighter (>~ 1 mag) than Galactic open clusters of similar age. Its present-day mass and half-light radius (r_h=7.4 pc) are more typical of faint globular clusters than of open clusters. However, given its position within the disk of M31 it is expected to be destroyed by dynamical effects, in particular by encounters with giant molecular clouds, within the next ~ 4 Gyr.Comment: 18 pages, 16 figures (quality of Figures 1,2,3 and 4 reduced). Accepted for publication in Astronomy & Astrophysics. Minor revisions to sect. 1.

Comparison of different delivery systems of DNA vaccination for the induction of protection against tuberculosis in mice and guinea pigs

The great challenges for researchers working in the field of vaccinology are optimizing DNA vaccines for use in humans or large animals and creating effective single-dose vaccines using appropriated controlled delivery systems. Plasmid DNA encoding the heat-shock protein 65 (hsp65) (DNAhsp65) has been shown to induce protective and therapeutic immune responses in a murine model of tuberculosis (TB). Despite the success of naked DNAhsp65-based vaccine to protect mice against TB, it requires multiple doses of high amounts of DNA for effective immunization. In order to optimize this DNA vaccine and simplify the vaccination schedule, we coencapsulated DNAhsp65 and the adjuvant trehalose dimycolate (TDM) into biodegradable poly (DL-lactide-co-glycolide) (PLGA) microspheres for a single dose administration. Moreover, a single-shot prime-boost vaccine formulation based on a mixture of two different PLGA microspheres, presenting faster and slower release of, respectively, DNAhsp65 and the recombinant hsp65 protein was also developed. These formulations were tested in mice as well as in guinea pigs by comparison with the efficacy and toxicity induced by the naked DNA preparation or BCG. The single-shot prime-boost formulation clearly presented good efficacy and diminished lung pathology in both mice and guinea pigs