78 research outputs found

    Pt-Sn/C as a possible methanol-tolerant cathode catalyst for DMFC

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    An effective method was developed for preparing highly dispersed nano-sized Pt–Sn/C electrocatalyst synthesised by a modified polyol reduction method. From XRD patterns, the Pt–Sn/C peaks shifted slightly to lower 2θ angles when compared with commercial Pt/C catalyst, suggesting that Sn formed alloy with Pt. Based on HR-TEM images, the Pt–Sn/C nanoparticles showed small particle sizes and well dispersed onto the carbon support with a narrow particle distribution. The methanol oxidation reaction on the as-prepared Pt–Sn/C catalyst appeared at lower currents (+7.08 mA at +480 mV vs. Ag/AgCl) compared to the commercial Pt/C (+8.25 mA at +480 mV vs. Ag/AgCl) suggesting that the Pt–Sn/C catalyst has ‘methanol tolerance capabilities’. Pt–Sn/C HA Slurry pH3 catalysts showed better activity towards the oxygen-reduction reaction (ORR) than commercial Pt/C which could be attributed to smaller particle sizes. In our study, the Pt–Sn/C catalyst appears to be a promising methanol-tolerant catalyst with activity towards the ORR in the DMFC.Web of Scienc

    Evaluation of a novel real-time PCR test based on the ssrA gene for the identification of group B streptococci in vaginal swabs

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    <p>Abstract</p> <p>Background</p> <p>Despite the implementation of prevention guidelines, early-onset group B streptococci (GBS) disease remains a cause of neonatal morbidity and mortality worldwide. Strategies to identify women who are at risk of transmitting GBS to their infant and the administration of intrapartum antibiotics have greatly reduced the incidence of neonatal GBS disease. However, there is a requirement for a rapid diagnostic test for GBS that can be carried out in a labour ward setting especially for women whose GBS colonisation status is unknown at the time of delivery. We report the design and evaluation of a real-time PCR test (<it>RiboSEQ </it>GBS test) for the identification of GBS in vaginal swabs from pregnant women.</p> <p>Methods</p> <p>The qualitative real-time PCR <it>RiboSEQ </it>GBS test was designed based on the bacterial <it>ssrA </it>gene and incorporates a competitive internal standard control. The analytical sensitivity of the test was established using crude lysate extracted from serial dilutions of overnight GBS culture using the IDI Lysis kit. Specificity studies were performed using DNA prepared from a panel of GBS strains, related streptococci and other species found in the genital tract environment. The <it>RiboSEQ </it>GBS test was evaluated on 159 vaginal swabs from pregnant women and compared with the GeneOhm™ StrepB Assay and culture for the identification of GBS.</p> <p>Results</p> <p>The <it>RiboSEQ </it>GBS test is specific and has an analytical sensitivity of 1-10 cell equivalents. The <it>RiboSEQ </it>GBS test was 96.4% sensitive and 95.8% specific compared to "gold standard" culture for the identification of GBS in vaginal swabs from pregnant women. In this study, the <it>RiboSEQ </it>GBS test performed slightly better than the commercial BD GeneOhm™ StrepB Assay which gave a sensitivity of 94.6% and a specificity of 89.6% compared to culture.</p> <p>Conclusion</p> <p>The <it>RiboSEQ </it>GBS test is a valuable method for the rapid, sensitive and specific detection of GBS in pregnant women. This study also validates the <it>ssrA </it>gene as a suitable and versatile target for nucleic acid-based diagnostic tests for bacterial pathogens.</p

    The Physics of the B Factories

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    This work is on the Physics of the B Factories. Part A of this book contains a brief description of the SLAC and KEK B Factories as well as their detectors, BaBar and Belle, and data taking related issues. Part B discusses tools and methods used by the experiments in order to obtain results. The results themselves can be found in Part C

    The Physics of the B Factories

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    Essai sur la toxicité de quelques Champignons avant et après leur dessiccation, par le Dr Ch. Cordier

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    Convert B.-H. Essai sur la toxicité de quelques Champignons avant et après leur dessiccation, par le Dr Ch. Cordier. In: Annales de la Société botanique de Lyon, tome 24, Notes et Mémoires - Comptes-rendus des séances – 1899. 3-4e trimestres, 1899. pp. 148-150

    Herborisation du 23 Juin 1895 dans la plaine d'Ambronay (Ain)

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    Convert B.-H. Herborisation du 23 Juin 1895 dans la plaine d'Ambronay (Ain). In: Annales de la Société botanique de Lyon, tome 20, Notes et Mémoires - Comptes-rendus des séances – 1895. 2e trimestre, 1895. pp. 45-47

    Essai sur la toxicité de quelques Champignons avant et après leur dessiccation, par le Dr Ch. Cordier

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    Convert B.-H. Essai sur la toxicité de quelques Champignons avant et après leur dessiccation, par le Dr Ch. Cordier. In: Annales de la Société botanique de Lyon, tome 24, Notes et Mémoires - Comptes-rendus des séances – 1899. 3-4e trimestres, 1899. pp. 148-150
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