Multi-modelling predictions show high uncertainty of required carbon input changes to reach a 4‰ target

Soils store vast amounts of carbon (C) on land, and increasing soil organic carbon (SOC) stocks in already managed soils such as croplands may be one way to remove C from the atmosphere, thereby limiting subsequent warming. The main objective of this study was to estimate the amount of additional C input needed to annually increase SOC stocks by 4%(0) at 16 long-term agricultural experiments in Europe, including exogenous organic matter (EOM) additions. We used an ensemble of six SOC models and ran them under two configurations: (1) with default parametrization and (2) with parameters calibrated site-by-site to fit the evolution of SOC stocks in the control treatments (without EOM). We compared model simulations and analysed the factors generating variability across models. The calibrated ensemble was able to reproduce the SOC stock evolution in the unfertilised control treatments. We found that, on average, the experimental sites needed an additional 1.5 +/- 1.2 Mg C ha(-)(1) year(-1) to increase SOC stocks by 4%(0) per year over 30 years, compared to the C input in the control treatments (multi-model median +/- median standard deviation across sites). That is, a 119% increase compared to the control. While mean annual temperature, initial SOC stocks and initial C input had a significant effect on the variability of the predicted C input in the default configuration (i.e., the relative standard deviation of the predicted C input from the mean), only water-related variables (i.e., mean annual precipitation and potential evapotranspiration) explained the divergence between models when calibrated. Our work highlights the challenge of increasing SOC stocks in agriculture and accentuates the need to increasingly lean on multi-model ensembles when predicting SOC stock trends and related processes. To increase the reliability of SOC models under future climate change, we suggest model developers to better constrain the effect of water-related variables on SOC decomposition

Towards a simple global-standard bioassay for a key ecosystem process: organic-matter decomposition using cotton strips

Cotton-strip bioassays are increasingly used to assess ecosystem integrity because they provide a standardized measure of organic-matter decomposition – a fundamental ecosystem process. However, several different cotton- strip assays are routinely used, complicating the interpretation of results across studies, and hindering broader synthesis. Here, we compare the decay rates and assemblages of bacteria and fungi colonizing the three most commonly used cotton materials: Artist’s canvas, Calico cloth, and Empa fabric. Cotton strips from each material type were incubated in 10 streams that span a wide range of physicochemical properties across five ecoregions. Additionally, to evaluate responses to environmental stress without potentially confounding biogeographical effects, we deployed identical bioassays in five streams across an acidification gradient within a single ecoregion. Across all streams decomposition rates (as tensile strength loss [TSL]) differed among the three cotton ma- terials; Calico cloth decomposed fastest (time to 50% TSL [T50]=16.7d), followed by the Empa fabric (T50 = 18.3 d) and then Artist’s canvas (T50 = 21.4 d). Despite these differences, rates of TSL of the three cotton materials responded consistently to variation in environmental conditions; TSL of each fabric increased with stream temperature, dissolved-nutrient concentrations and acid-neutralizing capacity, although Artist’s canvas and Calico cloth were more sensitive than Empa fabric. Microbial communities were similar among the mate- rials, and values of community structure (e.g., phylotype richness and diversity) were comparable to those reported for decaying leaves in streams from the same region, the major natural basal carbon resource in forested-stream ecosystems. We present linear calibrations among pairs of assays so that past and future studies can be expressed in a “common currency” (e.g., Artist’s-fabric equivalents) ‘past and future studies’ repeated two times in the sentence. Lastly, given its relatively low within-site variability, and the large number of streams where it has been used (> 700 across the globe), we recommend Artist’s fabric for future work. These results show that cotton provides an effective and realistic standardized substrate for studying heterotrophic microbial assemblages, and acts as a reasonable proxy for more chemically complex forms of detritus. These findings add to growing evidence that cotton-strip bioassays are simple, effective and easily standardized indicators of het- erotrophic microbial activity and the ecosystem processes that result

Non-target impact of fungicide tetraconazole on microbial communities in soils with different agricultural management

Effect of the fungicide tetraconazole on microbial community in silt loam soils from orchard with long history of triazole application and from grassland with no known history of fungicide usage was investigated. Triazole tetraconazole that had never been used on these soils before was applied at the field rate and at tenfold the FR. Response of microbial communities to tetraconazole was investigated during 28-day laboratory experiment by determination of changes in their biomass and structure (phospholipid fatty acids method—PLFA), activity (fluorescein diacetate hydrolysis—FDA) as well as changes in genetic (DGGE) and functional (Biolog) diversity. Obtained results indicated that the response of soil microorganisms to tetraconazole depended on the management of the soils. DGGE patterns revealed that both dosages of fungicide affected the structure of bacterial community and the impact on genetic diversity and richness was more prominent in orchard soil. Values of stress indices—the saturated/monounsaturated PLFAs ratio and the cyclo/monounsaturated precursors ratio, were almost twice as high and the Gram-negative/Gram-positive ratio was significantly lower in the orchard soil compared with the grassland soil. Results of principal component analysis of PLFA and Biolog profiles revealed significant impact of tetraconazole in orchard soil on day 28, whereas changes in these profiles obtained for grassland soil were insignificant or transient. Obtained results indicated that orchards soil seems to be more vulnerable to tetraconazole application compared to grassland soil. History of pesticide application and agricultural management should be taken into account in assessing of environmental impact of studied pesticides. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s10646-016-1661-7) contains supplementary material, which is available to authorized users

Additional carbon inputs to reach a 4 per 1000 objective in Europe: feasibility and projected impacts of climate change based on Century simulations of long-term arable experiments

International audienceAbstract. The 4 per 1000 initiative aims to maintain and increase soil organic carbon (SOC) stocks for soil fertility, food security, and climate change adaptation and mitigation. One way to enhance SOC stocks is to increase carbon (C) inputs to the soil. In this study, we assessed the amount of organic C inputs that are necessary to reach a target of SOC stocks increase by 4 ‰ yr−1 on average, for 30 years, at 14 long-term agricultural sites in Europe. We used the Century model to simulate SOC stocks and assessed the required level of additional C inputs to reach the 4 per 1000 target at these sites. Then, we analyzed how this would change under future scenarios of temperature increase. Initial stocks were simulated assuming steady state. We compared modeled C inputs to different treatments of additional C used on the experimental sites (exogenous organic matter addition and one treatment with different crop rotations). The model was calibrated to fit the control plots, i.e. conventional management without additional C inputs from exogenous organic matter or changes in crop rotations, and was able to reproduce the SOC stock dynamics. We found that, on average among the selected experimental sites, annual C inputs will have to increase by 43.15 ± 5.05 %, which is 0.66 ± 0.23 MgCha-1yr-1 (mean ± standard error), with respect to the initial C inputs in the control treatment. The simulated amount of C input required to reach the 4 ‰ SOC increase was lower than or similar to the amount of C input actually used in the majority of the additional C input treatments of the long-term experiments. However, Century might be overestimating the effect of additional C inputs on SOC stocks. At the experimental sites, we found that treatments with additional C inputs were increasing by 0.25 % on average. This means that the C inputs required to reach the 4 per 1000 target might actually be much higher. Furthermore, we estimated that annual C inputs will have to increase even more due to climate warming, that is 54 % more and 120 % more for a 1 and 5 ∘C warming, respectively. We showed that modeled C inputs required to reach the target depended linearly on the initial SOC stocks, raising concern on the feasibility of the 4 per 1000 objective in soils with a higher potential contribution to C sequestration, that is soils with high SOC stocks. Our work highlights the challenge of increasing SOC stocks at a large scale and in a future with a warmer climate

Phosphorus availability modulates the toxic effect of silver on aquatic fungi and leaf litter decomposition

International audienceThe functioning of forested headwater streams is intimately linked to the decomposition of leaf litter by decomposers, mainly aquatic hyphomycetes, which enables the transfer of allochthonous carbon to higher trophic levels. Evaluation of this process is being increasingly used as an indicator of ecosystem health and ecological integrity. Yet, even though the individual impacts of contaminants and nutrient availability on decomposition have been well studied, the understanding of their combined effects remains limited. In the current study, we investigated whether the toxic effects of a reemerging contaminant, silver (Ag), on leaf litter decomposition could be partly overcome in situations where microorganisms were benefitting from high phosphorus (P) availability, the latter being a key chemical element that often limits detritus decomposition. We also investigated whether these interactive effects were mediated by changes in the structure of the aquatic hyphomycete community. To verify these hypotheses, leaf litter decomposition by a consortium of ten aquatic hyphomycete species was followed in a microcosm experiment combining five Ag contamination levels and three P concentrations. Indirect effects of Ag and P on the consumption of leaf litter by the detritivorous crustacean, Gammarus fossarum, were also evaluated. Ag significantly reduced decomposition but only at the highest concentration tested, independently of P level. By contrast, P and Ag interactively affected fungal biomass. Both P level and Ag concentrations shaped microbial communities without significantly affecting the overall species richness. Finally, the levels of P and Ag interacted significantly on G. fossarum feeding rates, high [Ag] reducing litter consumption and low P availability tending to intensify the feeding rate. Given the high level of contaminant needed to impair the decomposition process, it is unlikely that a direct effect of Ag on leaf litter decomposition could be observed in situ. However, subtle Ag effects in relation to nutrient levels in ecosystems could be expected. In particular, owing to higher consumption of low P leaf litter, shredding invertebrates could increase the ingestion of contaminated resources, which could, in turn, represent an important threat to headwater stream ecosystems

The Seine watershed water-agro-food system: long-term trajectories of C, N, P metabolism

International audienceBased on the GRAFS method of biogeochemical accounting for nitrogen (N), phosphorus (P) and carbon (C) fluxes through crop, grassland, livestock and human consumption, a full description of the structure and main functioning features of the French agro-food system was obtained from 1850 to the present at the scale of 33 agricultural regions. For the period since 1970, this description was compared with the results of an agronomic reconstitution of the cropping systems of the Seine watershed based on agricultural census and detailed enquiries about farming practices at the scale of small agricultural regions (the ARSeine database), which were then used as input to an agronomical model (STICS) calculating yields, and the dynamics of N and C. STICS was then coupled with a hydrogeological model (MODCOU), so that the entire modelling chain can thus highlight the high temporal inertia of both soil organic matter pool and aquifers. GRAFS and ARSeine revealed that the agriculture of the North of France is currently characterised by a high degree of territorial openness, specialisation and disconnection between crop and livestock farming, food consumption and production. This situation is the result of a historical trajectory starting in the middle of the nineteenth century, when agricultural systems based on mixed crop and livestock farming with a high level of autonomy were dominant. The major transition occurred only after World War II and the implementation of the Common Agricultural Policy and led, within only a few decades, to a situation where industrial fertilisers largely replaced manure and where livestock farming activities were concentrated either in the Eastern margins of the watershed in residual mixed farming areas or in specialised animal production zones of the Great West. A second turning point occurred around the 1990s when regulatory measures were taken to partly correct the environmental damage caused by the preceding regime, yet without in-depth change of its logic of specialisation and intensification. Agricultural soil biogeochemistry (C sequestration, nitrate losses, P accumulation, etc.) responds, with a long delay, to these long-term structural changes. The same is true for the hydrosystem and most of its different compartments (vadose zone, aquifers, riparian zones), so that the relationship between the diffuse sources of nutrients (or pesticides) and the agricultural practices is not immediate and is strongly influenced by legacies from the past structure and practices of the agricultural system. This has strong implications regarding the possible futures of the Seine basin agriculture