10 research outputs found
"An adventure that went wrong": Reasons given by convicted perpetrators of multiple perpetrator sexual offending for their involvement in the offense
Finding a Needle in the Virus Metagenome Haystack - Micro-Metagenome Analysis Captures a Snapshot of the Diversity of a Bacteriophage Armoire
Viruses are ubiquitous in the oceans and critical components of marine microbial communities, regulating nutrient transfer to higher trophic levels or to the dissolved organic pool through lysis of host cells. Hydrothermal vent systems are oases of biological activity in the deep oceans, for which knowledge of biodiversity and its impact on global ocean biogeochemical cycling is still in its infancy. In order to gain biological insight into viral communities present in hydrothermal vent systems, we developed a method based on deep-sequencing of pulsed field gel electrophoretic bands representing key viral fractions present in seawater within and surrounding a hydrothermal plume derived from Loki's Castle vent field at the Arctic Mid-Ocean Ridge. The reduction in virus community complexity afforded by this novel approach enabled the near-complete reconstruction of a lambda-like phage genome from the virus fraction of the plume. Phylogenetic examination of distinct gene regions in this lambdoid phage genome unveiled diversity at loci encoding superinfection exclusion- and integrase-like proteins. This suggests the importance of fine-tuning lyosgenic conversion as a viral survival strategy, and provides insights into the nature of host-virus and virus-virus interactions, within hydrothermal plumes. By reducing the complexity of the viral community through targeted sequencing of prominent dsDNA viral fractions, this method has selectively mimicked virus dominance approaching that hitherto achieved only through culturing, thus enabling bioinformatic analysis to locate a lambdoid viral “needle" within the greater viral community “haystack". Such targeted analyses have great potential for accelerating the extraction of biological knowledge from diverse and poorly understood environmental viral communities
Pan genome of the phytoplankton Emiliania underpins its global distribution
Coccolithophores have influenced the global climate for over 200
million years1. These marine phytoplankton can account for 20 per
cent of total carbon fixation in some systems2. They form blooms
that can occupy hundreds of thousands of square kilometres and
are distinguished by their elegantly sculpted calcium carbonate exoskeletons
(coccoliths), rendering themvisible fromspace3.Although
coccolithophores export carbon in the form of organic matter and
calcite to the sea floor, they also release CO2 in the calcification
process. Hence, they have a complex influence on the carbon cycle,
driving either CO2 production or uptake, sequestration and export
to the deep ocean4. Here we report the first haptophyte reference
genome, from the coccolithophore Emiliania huxleyi strain
CCMP1516, and sequences from 13 additional isolates. Our analyses
reveal a pan genome (core genes plus genes distributed variably
between strains) probably supported by an atypical complement
of repetitive sequence in the genome. Comparisons across strains
demonstrate thatE. huxleyi, which has long been considered a single
species, harbours extensive genome variability reflected in different
metabolic repertoires. Genome variability within this species
complex seems to underpin its capacity both to thrive in habitats
ranging from the equator to the subarctic and to form large-scale
episodic blooms under a wide variety of environmental conditions
Transcriptional Regulation by Asf1: NEW MECHANISTIC INSIGHTS FROM STUDIES OF THE DNA DAMAGE RESPONSE TO REPLICATION STRESS*
Asf1 is a conserved histone H3/H4 chaperone. We find that Asf1 in budding yeast promotes an essential cellular response to replication stress caused by the ribonucleotide reductase inhibitor hydroxyurea. That is, Asf1 stimulates derepression of DNA damage response (DDR) genes during the S phase. Derepression of DDR genes strongly correlates with Asf1 binding to their promoters. Having identified the C terminus and histone-binding domains of Asf1 as molecular determinants of its constitutive and inducible association with chromatin, we tested whether Asf1 binding to DDR genes is mechanistically important for their derepression. Our results provide little support for this hypothesis. Rather, the contribution of Asf1 to DDR gene derepression depends on its ability to stimulate H3K56 acetylation by lysine acetyltransferase Rtt109. The precise regulation of H3K56 acetylation in the promoters of DDR genes is unexpected: DDR gene promoters are occupied by H3K56-acetylated nucleosomes under repressing conditions, and the steady state level of H3K56 promoter acetylation does not change upon derepression. We propose that replication-coupled deposition of Lys56-acetylated H3 poises the DDR genes in newly synthesized daughter duplexes for derepression during the S phase. In this model, the presence of a histone mark that destabilizes nucleosomes is compatible with suppression of transcription because in the uninduced state, DDR gene promoters are constitutively occupied by a potent repressor-corepressor complex