22 research outputs found
Different Transcript Patterns in Response to Specialist and Generalist Herbivores in the Wild Arabidopsis Relative Boechera divaricarpa
BACKGROUND: Plants defend themselves against herbivorous insects, utilizing both constitutive and inducible defenses. Induced defenses are controlled by several phytohormone-mediated signaling pathways. Here, we analyze transcriptional changes in the North American Arabidopsis relative Boechera divaricarpa in response to larval herbivory by the crucifer specialist lepidopteran Plutella xylostella (diamondback moth) and by the generalist lepidopteran Trichoplusia ni (cabbage semilooper), and compare them to wounding and exogenous phytohormone application. METHODOLOGY/PRINCIPAL FINDINGS: We use a custom macroarray constructed from B. divaricarpa herbivory-regulated cDNAs identified by suppression subtractive hybridization and from known stress-responsive A. thaliana genes for transcript profiling after insect herbivory, wounding and in response to jasmonate, salicylate and ethylene. In addition, we introduce path analysis as a novel approach to analyze transcript profiles. Path analyses reveal that transcriptional responses to the crucifer specialist P. xylostella are primarily determined by direct effects of the ethylene and salicylate pathways, whereas responses to the generalist T. ni are influenced by the ethylene and jasmonate pathways. Wound-induced transcriptional changes are influenced by all three pathways, with jasmonate having the strongest effect. CONCLUSIONS/SIGNIFICANCE: Our results show that insect herbivory is distinct from simple mechanical plant damage, and that different lepidopteran herbivores elicit different transcriptional responses
Upper limit on the cosmic-ray photon fraction at EeV energies from the Pierre Auger Observatory
From direct observations of the longitudinal development of ultra-high energy
air showers performed with the Pierre Auger Observatory, upper limits of 3.8%,
2.4%, 3.5% and 11.7% (at 95% c.l.) are obtained on the fraction of cosmic-ray
photons above 2, 3, 5 and 10 EeV (1 EeV = 10^18 eV) respectively. These are the
first experimental limits on ultra-high energy photons at energies below 10
EeV. The results complement previous constraints on top-down models from array
data and they reduce systematic uncertainties in the interpretation of shower
data in terms of primary flux, nuclear composition and proton-air
cross-section.Comment: 20 pages, 7 figures, 2 tables. Minor changes. Accepted by
Astroparticle Physic