Improving conservation and translocation success of an endangered orchid, Caladenia xanthochila (Orchidaceae), through understanding pollination

Critical for conserving endangered orchids is identifying their pollinators and their distribution. Caladenia xanthochila is an endangered orchid that has floral traits characteristic of pollination by food foraging insects. We identified the pollinator(s), mechanisms of attraction and the presence of pollinators at natural, existing and potential translocation sites. Furthermore, we quantified pollination success at translocation sites and investigated the effect of rainfall on pollination success over 19 years at a natural site. We clarify if sharing of pollinators occurs with closely related species by comparing the CO1 barcoding region of the pollinators' DNA. Caladenia xanthochila was pollinated by a single species of thynnine wasp, Phymatothynnus aff. nitidus. Caladenia xanthochila produced 27.0 µg ± 7.1 sucrose on the labellum, while pollinators vigorously copulated with glandular clubs on the sepal tips, suggestive of a mixed pollination system. Pollination success of C. xanthochila was 7.6 ± 1.5% SE at the natural site and 16.1 ± 3.6% SE across the translocation sites. Furthermore, hand pollinations demonstrated that pollination was pollen limited. Pollination success was significantly related to average rainfall during the growth phase of the orchid (P < 0.001). Potential translocation sites for C. xanthochila were limited, with four of six surveyed lacking the pollinator. We found evidence for cryptic species of Phymatothynnus, with C. xanthochila pollinators being unique amongst the orchids studied. We recommend hand pollinations at translocated and remnant wild populations to boost initial recruitment. The evidence for cryptic species of pollinators further highlights the need for accurate identification of pollinators

Steering Mirror System with Closed-Loop Feedback for Free-Space Optical Communication Terminals

Precision beam pointing plays a critical role in free-space optical communications terminals in uplink, downlink and inter-satellite link scenarios. Among the various methods of beam steering, the use of fast steering mirrors (FSM) is widely adopted, with many commercial solutions employing diverse technologies, particularly focusing on small, high-bandwidth mirrors. This paper introduces a method using lightweight, commercial off-the-shelf components to construct a custom closed-loop steering mirror platform, suitable for mirror apertures exceeding 100 mm. The approach involves integrating optical encoders into two off-the-shelf open-loop actuators. These encoders read the signal reflected on purposefully diamond-machined knurled screw knobs, providing maximum contrast between light and dark lines. The resulting steering mirror has the potential to complement or replace FSM in applications requiring a larger stroke, at the expense of motion speed. In the presented setup, the mirror tilt resolution achieved based on the encoder closed-loop signal feedback is 45 μrad, with a mean slew rate of 1.5 mrad/s. Importantly, the steering assembly is self-locking, requiring no power to maintain a steady pointing angle. Using the mirror to actively correct for a constantly moving incoming beam, a 5-fold increase in concentration of the beam spot on the center of the detector was obtained compared to a fixed position mirror, demonstrating the mirrors ability to correct for satellite platform jitter and drift

Monte–Carlo simulation of crystallization in single‐chain square‐well homopolymers

© 2020 Wiley Periodicals LLC We present Monte–Carlo (MC) simulations of the crystallization transition of single-chain square-well homopolymers, with a continuous description of monomer positions. For long chains with short-ranged interactions this system shows a strong configurational bottleneck, which makes it difficult to explore the whole configuration space. To surmount this problem we combine parallel tempering with a nonstandard choice of tempering levels, a bespoke biasing strategy and a method to map results between different temperatures. We verify that our simulations mix well when simulating chains of 128 and 256 beads. Our simulation approach resolves issues with reproducibility of MC simulations reported in prior work, particularly for the transition region between the expanded coil and crystalline region. We obtain highly reproducible results for both the free energy landscape and the inverse temperature, with low statistical noise. We outline a method to extract the free energy barrier, at any temperature, for any choice of order parameter, illustrating this technique by computing the free energy landscape as a function of the Steinhardt–Nelson order parameter for a range of temperatures

eMouseAtlas, EMAGE, and the spatial dimension of the transcriptome

Abstract eMouseAtlas (www.emouseatlas.org) is a com-prehensive online resource to visualise mouse development and investigate gene expression in the mouse embryo. We have recently deployed a completely redesigned Mouse Anatomy Atlas website (www.emouseatlas.org/emap/ema) that allows users to view 3D embryo reconstructions, delineated anatomy, and high-resolution histological sec-tions. A new feature of the website is the IIP3D web tool that allows a user to view arbitrary sections of 3D embryo reconstructions using a web browser. This feature provides interactive access to very high-volume 3D images via a tiled pan-and-zoom style interface and circumvents the need to download large image files for visualisation. eMouseAtla

A nonlinear theory of the parallel firehose and gyrothermal instabilities in a weakly collisional plasma

Weakly collisional plasmas dynamically develop pressure anisotropies with respect to the magnetic field. These anisotropies trigger plasma instabilities at scales just above the ion Larmor radius \rho_i and much below the mean free path \lambda_{mfp}. They have growth rates of a fraction of the ion cyclotron frequency - much faster than either the global dynamics or local turbulence. The instabilities dramatically modify the transport properties and, therefore, the macroscopic dynamics of the plasma. Their nonlinear evolution drives pressure anisotropies towards marginal stability, controlled by the plasma beta \beta_i. Here this nonlinear evolution is worked out for the simplest analytically tractable example - the parallel firehose instability. In the nonlinear regime, both analytical theory and the numerical solution predict secular growth of magnetic fluctuations. They develop a k^{-3} spectrum, extending from scales somewhat larger than \rho_i to the maximum scale that grows secularly with time (~t^{1/2}); the relative pressure anisotropy (\pperp-\ppar)/\ppar tends to the marginal value -2/\beta_i. The marginal state is achieved via changes in the magnetic field, not particle scattering. When a parallel ion heat flux is present, the firehose mutates into the new gyrothermal instability (GTI), which continues to exist up to firehose-stable values of pressure anisotropy, which can be positive and are limited by the heat flux. The nonlinear evolution of the GTI also features secular growth of magnetic fluctuations, but the spectrum is eventually dominated by modes around the scale ~\rho_i l_T/\lambda_{mfp}, where l_T is the scale of the parallel temperature variation. Implications for momentum and heat transport are speculated about. This study is motivated by the dynamics of galaxy cluster plasmas.Comment: 34 pages, replaced with the version published in MNRA

Solar Wind Turbulence and the Role of Ion Instabilities

International audienc

Cognitive behavioural therapy for adults with dissociative seizures (CODES): a pragmatic, multicentre, randomised controlled trial.

BACKGROUND: Dissociative seizures are paroxysmal events resembling epilepsy or syncope with characteristic features that allow them to be distinguished from other medical conditions. We aimed to compare the effectiveness of cognitive behavioural therapy (CBT) plus standardised medical care with standardised medical care alone for the reduction of dissociative seizure frequency. METHODS: In this pragmatic, parallel-arm, multicentre randomised controlled trial, we initially recruited participants at 27 neurology or epilepsy services in England, Scotland, and Wales. Adults (≥18 years) who had dissociative seizures in the previous 8 weeks and no epileptic seizures in the previous 12 months were subsequently randomly assigned (1:1) from 17 liaison or neuropsychiatry services following psychiatric assessment, to receive standardised medical care or CBT plus standardised medical care, using a web-based system. Randomisation was stratified by neuropsychiatry or liaison psychiatry recruitment site. The trial manager, chief investigator, all treating clinicians, and patients were aware of treatment allocation, but outcome data collectors and trial statisticians were unaware of treatment allocation. Patients were followed up 6 months and 12 months after randomisation. The primary outcome was monthly dissociative seizure frequency (ie, frequency in the previous 4 weeks) assessed at 12 months. Secondary outcomes assessed at 12 months were: seizure severity (intensity) and bothersomeness; longest period of seizure freedom in the previous 6 months; complete seizure freedom in the previous 3 months; a greater than 50% reduction in seizure frequency relative to baseline; changes in dissociative seizures (rated by others); health-related quality of life; psychosocial functioning; psychiatric symptoms, psychological distress, and somatic symptom burden; and clinical impression of improvement and satisfaction. p values and statistical significance for outcomes were reported without correction for multiple comparisons as per our protocol. Primary and secondary outcomes were assessed in the intention-to-treat population with multiple imputation for missing observations. This trial is registered with the International Standard Randomised Controlled Trial registry, ISRCTN05681227, and ClinicalTrials.gov, NCT02325544. FINDINGS: Between Jan 16, 2015, and May 31, 2017, we randomly assigned 368 patients to receive CBT plus standardised medical care (n=186) or standardised medical care alone (n=182); of whom 313 had primary outcome data at 12 months (156 [84%] of 186 patients in the CBT plus standardised medical care group and 157 [86%] of 182 patients in the standardised medical care group). At 12 months, no significant difference in monthly dissociative seizure frequency was identified between the groups (median 4 seizures [IQR 0-20] in the CBT plus standardised medical care group vs 7 seizures [1-35] in the standardised medical care group; estimated incidence rate ratio [IRR] 0·78 [95% CI 0·56-1·09]; p=0·144). Dissociative seizures were rated as less bothersome in the CBT plus standardised medical care group than the standardised medical care group (estimated mean difference -0·53 [95% CI -0·97 to -0·08]; p=0·020). The CBT plus standardised medical care group had a longer period of dissociative seizure freedom in the previous 6 months (estimated IRR 1·64 [95% CI 1·22 to 2·20]; p=0·001), reported better health-related quality of life on the EuroQoL-5 Dimensions-5 Level Health Today visual analogue scale (estimated mean difference 6·16 [95% CI 1·48 to 10·84]; p=0·010), less impairment in psychosocial functioning on the Work and Social Adjustment Scale (estimated mean difference -4·12 [95% CI -6·35 to -1·89]; p<0·001), less overall psychological distress than the standardised medical care group on the Clinical Outcomes in Routine Evaluation-10 scale (estimated mean difference -1·65 [95% CI -2·96 to -0·35]; p=0·013), and fewer somatic symptoms on the modified Patient Health Questionnaire-15 scale (estimated mean difference -1·67 [95% CI -2·90 to -0·44]; p=0·008). Clinical improvement at 12 months was greater in the CBT plus standardised medical care group than the standardised medical care alone group as reported by patients (estimated mean difference 0·66 [95% CI 0·26 to 1·04]; p=0·001) and by clinicians (estimated mean difference 0·47 [95% CI 0·21 to 0·73]; p<0·001), and the CBT plus standardised medical care group had greater satisfaction with treatment than did the standardised medical care group (estimated mean difference 0·90 [95% CI 0·48 to 1·31]; p<0·001). No significant differences in patient-reported seizure severity (estimated mean difference -0·11 [95% CI -0·50 to 0·29]; p=0·593) or seizure freedom in the last 3 months of the study (estimated odds ratio [OR] 1·77 [95% CI 0·93 to 3·37]; p=0·083) were identified between the groups. Furthermore, no significant differences were identified in the proportion of patients who had a more than 50% reduction in dissociative seizure frequency compared with baseline (OR 1·27 [95% CI 0·80 to 2·02]; p=0·313). Additionally, the 12-item Short Form survey-version 2 scores (estimated mean difference for the Physical Component Summary score 1·78 [95% CI -0·37 to 3·92]; p=0·105; estimated mean difference for the Mental Component Summary score 2·22 [95% CI -0·30 to 4·75]; p=0·084), the Generalised Anxiety Disorder-7 scale score (estimated mean difference -1·09 [95% CI -2·27 to 0·09]; p=0·069), and the Patient Health Questionnaire-9 scale depression score (estimated mean difference -1·10 [95% CI -2·41 to 0·21]; p=0·099) did not differ significantly between groups. Changes in dissociative seizures (rated by others) could not be assessed due to insufficient data. During the 12-month period, the number of adverse events was similar between the groups: 57 (31%) of 186 participants in the CBT plus standardised medical care group reported 97 adverse events and 53 (29%) of 182 participants in the standardised medical care group reported 79 adverse events. INTERPRETATION: CBT plus standardised medical care had no statistically significant advantage compared with standardised medical care alone for the reduction of monthly seizures. However, improvements were observed in a number of clinically relevant secondary outcomes following CBT plus standardised medical care when compared with standardised medical care alone. Thus, adults with dissociative seizures might benefit from the addition of dissociative seizure-specific CBT to specialist care from neurologists and psychiatrists. Future work is needed to identify patients who would benefit most from a dissociative seizure-specific CBT approach. FUNDING: National Institute for Health Research, Health Technology Assessment programme

Employing the Gini coefficient to measure participation inequality in treatment-focused Digital Health Social Networks

Digital Health Social Networks (DHSNs) are common; however, there are few metrics that can be used to identify participation inequality. The objective of this study was to investigate whether the Gini coefficient, an economic measure of statistical dispersion traditionally used to measure income inequality, could be employed to measure DHSN inequality. Quarterly Gini coefficients were derived from four long-standing DHSNs. The combined data set included 625,736 posts that were generated from 15,181 actors over 18,671 days. The range of actors (8–2323), posts (29–28,684), and Gini coefficients (0.15–0.37) varied. Pearson correlations indicated statistically significant associations between number of actors and number of posts (0.527–0.835, p < .001), and Gini coefficients and number of posts (0.342–0.725, p < .001). However, the association between Gini coefficient and number of actors was only statistically significant for the addiction networks (0.619 and 0.276, p < .036). Linear regression models had positive but mixed R2 results (0.333–0.527). In all four regression models, the association between Gini coefficient and posts was statistically significant (t = 3.346–7.381, p < .002). However, unlike the Pearson correlations, the association between Gini coefficient and number of actors was only statistically significant in the two mental health networks (t = −4.305 and −5.934, p < .000). The Gini coefficient is helpful in measuring shifts in DHSN inequality. However, as a standalone metric, the Gini coefficient does not indicate optimal numbers or ratios of actors to posts, or effective network engagement. Further, mixed-methods research investigating quantitative performance metrics is required

Sexual Priming, Gender Stereotyping, and Likelihood to Sexually Harass: Examining the Cognitive Effects of Playing a Sexually-Explicit Video Game

The present study examines the short-term cognitive effects of playing a sexually explicit video game with female “objectification” content on male players. Seventy-four male students from a university in California, U.S. participated in a laboratory experiment. They were randomly assigned to play either a sexually-explicit game or one of two control games. Participants’ cognitive accessibility to sexual and sexually objectifying thoughts was measured in a lexical decision task. A likelihood-to-sexually-harass scale was also administered. Results show that playing a video game with the theme of female “objectification” may prime thoughts related to sex, encourage men to view women as sex objects, and lead to self-reported tendencies to behave inappropriately towards women in social situations