Life-cycle greenhouse gas emissions of portuguese olive oil

The main goal of this paper was to assess the greenhouse gas (GHG) intensity of olive oil production in Portugal. A life-cycle model and inventory were implemented for the entire production process, including a comprehensive analysis of olive cultivation, olive oil extraction, packaging, and distribution. Data originates from five differently-sized Portuguese olive growers and from a total of six olive oil mills, representing the three extraction processes in use: three-phase extraction, two-phase extraction, and traditional pressing. The results show that the GHG intensity lies in the range 1.8-8.2 kg CO2eq/liter and that the main contributors were fertilizers (production and field emissions). Efficient use of fertilizers thus seems to be a key factor for mitigating the GHG intensity of olive oil production.This research was supported by project ECODEEP (Eco-efficiency and Eco-management in the Agro Industrial sector, FCOMP–05–0128–FEDER–018643) and the Portuguese Scienc

Production of fructo-oligosaccharides by Aspergillus ibericus and their chemical characterization

A great demand for prebiotics is driving the search for new sources of fructo-oligosaccharides (FOS) producers and for FOS with differentiated functionalities. In the present work, FOS production by a new isolated strain of Aspergillus ibericus was evaluated. The temperature of fermentation and initial pH were optimized in shaken flask to yield a maximal FOS production, through a central composite experimental design. FOS were produced in a one-step bioprocess using the whole cells of the microorganism. The model (R2 = 0.918) predicted a yield of 0.56, experimentally 0.53 ± 0.03 gFOS.ginitial sucrose1 was obtained (37.0 °C and a pH of 6.2). A yield of 0.64 ± 0.02 gFOS.ginitial sucrose1 was obtained in the bioreactor, at 38 h, with a content of 118 ± 4 g.L1 in FOS and a purity of 56 ± 3%. The chemical structure of the FOS produced by A. ibericus was determined by HPLC and NMR. FOS were identified as 1-kestose, nystose, and 1F-fructofuranosylnystose. In conclusion, A. ibericus was found to be a good alternative FOS producer.Clarisse Nobre acknowledges the Portuguese Foundation forScience and Technology (FCT) for her Post-Doc Grant [ref. SFRH/BPD/87498/ 2012] and the project RECI/BBB-EBI/0179/2012 (FCOMP-01-0124FEDER-027462), the strategic funding of UID/BIO/04469/2013 unit, COMPETE 2020 (POCI-01-0145-FEDER-006684), BioTecNorte operation(NORTE-01-0145-FEDER-000004) and the project MultiBiorefinery (POCI-01-0145-FEDER-016403) funded by European Regional Development Fund under the scope of Norte2020 - Programa Operacional Regional do Norte.info:eu-repo/semantics/publishedVersio

An investigation of the potential application of chitosan/aloe-based membranes for regenerative medicine

A significant number of therapeutics derived from natural polymers and plants have been developed to replace or to be used in conjunction with existing dressing products. The use of the therapeutic properties of aloe vera could be very useful in the creation of active wound dressing materials. The present work was undertaken to examine issues concerning structural features, topography, enzymatic degradation behavior, antibacterial activity and cellular response of chitosan/aloe vera-based membranes. The chitosan/aloe vera-based membranes that were developed displayed satisfactory degradation, roughness, wettability and mechanical properties. A higher antibacterial potency was displayed by the blended membranes. Moreover, in vitro assays demonstrated that these blended membranes have good cell compatibility with primary human dermal fibroblasts. The chitosan/aloe vera-based membranes might be promising wound dressing materials.The authors acknowledge financial support from the Portuguese Foundation for Science and Technology (grants SFRH/BPD/45307/2008 and SFRH/BD/64601/2009), the "Fundo Social Europeu", and the "Programa Diferencial de Potencial Humano". This work was partially supported by the FEDER through POCTEP 0330_IBEROMARE_1_P

Expressão da esterilidade feminina e da carpeloidia em mamoeiro sob diferentes ambientes de cultivo protegido.

O presente estudo teve por objetivo avaliar a ocorrência de esterilidade feminina e de carpeloidia em mamoeiros hermafroditas 'Baixinho-de-Santa Amália' cultivados sob manejo orgânico, em diferentes tipos de ambiente de proteção,e conduzido com ou sem bifurcação do tronco no transcorrer das quatro estações do ano. Foram construídos três tipos de estruturas de proteção contíguas: (i) estufa (cobertura de plástico); (ii) estufa sombreada (cobertura adicional de tela 'sombrite' - 30% sobre o plástico), e (iii) telado (cobertura exclusiva de tela 'sombrite' - 30%), ao lado de uma área de ambiente natural, a pleno sol. Nestes locais, foram cultivados, dentro das normas técnicas da agricultura orgânica, mamoeiros da cv. Baixinho-de-Santa-Amália. Em metade das plantas, abrangendo todos os ambientes de cultivo, a gema apical foi incisada, logo após a sexagem, visando à bifurcação do tronco. Para efeito de análise de variância, foram considerados quatro blocos por ambiente de cultivo, tendo cada bloco três repetições relativas ao modo de condução das plantas (com e sem bifurcação do tronco). Para análise estatística, procedeu-se à "análise conjunta de experimentos", no caso, os ambientes de cultivo. Nos mamoeiros com tronco bifurcado, houve diminuição do número de frutos carpeloides e aumento do número de flores fêmeas estéreis. No entanto, essa bifurcação não influenciou a frequência de frutos normais. Durante a primavera (setembro a dezembro), e notadamente na estufa, o maior número de frutos carpeloides por planta correlacionou-se a temperaturas mais elevadas, maior amplitude térmica e maior vigor vegetativo; já, a maior ocorrência de flores estaminadas correlacionou-se também a temperaturas elevadas, baixa luminosidade e menor vigor vegetativo. Por outro lado, essas mesmas condições ambientais e fenológicas favoráveis à carpeloidia aumentaram a quantidade de frutos normais, assim contribuindo positivamente para a produtividade do mamoeiro

Measurement of the polarisation of W bosons produced with large transverse momentum in pp collisions at sqrt(s) = 7 TeV with the ATLAS experiment

This paper describes an analysis of the angular distribution of W->enu and W->munu decays, using data from pp collisions at sqrt(s) = 7 TeV recorded with the ATLAS detector at the LHC in 2010, corresponding to an integrated luminosity of about 35 pb^-1. Using the decay lepton transverse momentum and the missing transverse energy, the W decay angular distribution projected onto the transverse plane is obtained and analysed in terms of helicity fractions f0, fL and fR over two ranges of W transverse momentum (ptw): 35 < ptw < 50 GeV and ptw > 50 GeV. Good agreement is found with theoretical predictions. For ptw > 50 GeV, the values of f0 and fL-fR, averaged over charge and lepton flavour, are measured to be : f0 = 0.127 +/- 0.030 +/- 0.108 and fL-fR = 0.252 +/- 0.017 +/- 0.030, where the first uncertainties are statistical, and the second include all systematic effects.Comment: 19 pages plus author list (34 pages total), 9 figures, 11 tables, revised author list, matches European Journal of Physics C versio