106 research outputs found
Purification, characterization and molecular cloning of the major chitinase from Tenebrio molitor larval midgut
- Publication venue
- 'Elsevier BV'
- Publication date
- 01/10/2006
- Field of study
Insect chitinases are involved in degradation of chitin from the exoskeleton cuticle or from midgut peritrophic membrane during molts. cDNAs coding for insect cuticular and gut chitinases were cloned, but only chitinases from moulting fluid were purified and characterized. In this study the major digestive chitinase from T. molitor midgut (TmChi) was purified to homogeneity, characterized and sequenced after cDNA cloning. TmChi is secreted by midgut epithelial cells, has a molecular weight of 44 kDa and is unstable in the presence of midgut proteinases. TmChi shows strong substrate inhibition when acting on umbelliferyl-derivatives of chitobio- and chitotriosaccharides, but has normal Michaelis kinetics with the N-acetylglucosamine derivative as substrate. TmChi has very low activity against colloidal chitin, but effectively converts oligosaccharides to shorter fragments. The best substrate for TmChi is chitopentaose, with highest kcat/KM value. Sequence analysis and chemical modification experiments showed that the TmChi active site contains carboxylic groups and a tryptophane, which are known to be important for catalysis in family 18 chitinases. Modification with p-hidroximercuribenzoate of a cysteine residue, which is exposed after substrate binding, leads to complete inactivation of the enzyme. TmChi mRNA encodes a signal peptide plus a protein with 37 kDa and high similarity with other insect chitinases from family 18. Surprisingly, this gene does not encode the C-terminal Ser-Thr-rich connector and chitin-binding domain normally present in chitinases. The special features of TmChi probably result from its adaptation to digest chitin-rich food without damaging the peritrophic membrane. © 2006 Elsevier Ltd. All rights reserved
Alcoholism and Strongyloides stercoralis: Daily Ethanol Ingestion Has a Positive Correlation with the Frequency of Strongyloides Larvae in the Stools
- Author
- A Gonzalez-Quintela
- AF Porto
- AJ Krolewiecki
- AM Galioto
- C Waltenbaugh
- Carla Couzi Marques
- Carlos Sandoval Gonçalves
- CL Mendenhall
- CS Lieber
- CV Silva
- D Gaburri
- FA Neva
- Fausto Edmundo Lima Pereira
- G Addolorato
- G Szabo
- G Szabo
- K Ogilvie
- L Avendaño
- LA Brown
- LCM Oliveira
- LE Diaz
- M Wegener
- Maria da Penha Zago-Gomes
- ME Viney
- MJ Dominguez-Santalla
- MP Zago-Gomes
- MS Macieira
- O Adedayo
- Peter Steinmann
- R Marcial-Rojas
- RM Genta
- RT Cook
- SC Harvey
- UM Padigel
- Publication venue
- Public Library of Science
- Publication date
- 22/06/2010
- Field of study
It has been reported that Strongyloides stercoralis infection is more prevalent in chronic alcoholic patients than in non alcoholics living in the same country. In a retrospective study on the prevalence of S. stercoralis infection in a large sample of alcoholic patients, we demonstrate that this prevalence is significantly higher than in non-alcoholic patients admitted at the same hospital. Moreover, the frequency of the parasite was in close relationship with the daily amount of ingested ethanol, even in the absence of liver cirrhosis, reinforcing the idea that chronic alcoholism is associated with increased susceptibility to Strongyloides infection. Beside the bad hygiene profile of alcoholic patients, which explains high risk for acquisition of the parasite, the high prevalence of S. stercoralis in alcoholics may be in relationship with other effects of ethanol on the intestinal motility, steroid metabolism and immune system, which could enhance the chance of autoinfection and the survival and fecundity of females in duodenum. In this way, the number of larvae in the stools is higher in alcoholic patients, increasing the chance of a positive result in a stool examination by sedimentation method
Comparative Genomic Analysis of Chitinase and Chitinase-Like Genes in the African Malaria Mosquito (Anopheles gambiae)
- Author
- A Krishnan
- A Nakabachi
- C Khajuria
- Dominik Hartl
- E Cohen
- E Fitches
- Enbo Ma
- FA Genta
- H de la Vega
- H Merzendorfer
- H Merzendorfer
- J Zhang
- JD Funkhouser
- Jianzhen Zhang
- JM Ramalho-Ortigao
- JZ Zhang
- Karl J. Kramer
- KJ Kramer
- KJ Kramer
- Kun Yan Zhu
- L Shao
- L Shi
- L Shuhui
- M Matsumiya
- MF Moreira
- MG Kim
- MW Pfaffl
- Q Zhu
- Q Zhu
- Q Zhu
- Q Zhu
- R Bolognesi
- RB Kirkpatrick
- Subbaratnam Muthukrishnan
- T Ahmad
- T Daimon
- T Shinoda
- T Togawa
- TA Reese
- V Royer
- Xin Zhang
- Y Arakane
- Y Wei
- Y Zheng
- Yasuyuki Arakane
- Z Shen
- Publication venue
- Public Library of Science
- Publication date
- 18/05/2011
- Field of study
Chitinase is an important enzyme responsible for chitin metabolism in a wide range of organisms including bacteria, yeasts and other fungi, nematodes and arthropods. However, current knowledge on chitinolytic enzymes, especially their structures, functions and regulation is very limited. In this study we have identified 20 chitinase and chitinase-like genes in the African malaria mosquito, Anopheles gambiae, through genome-wide searching and transcript profiling. We assigned these genes into eight different chitinase groupings (groups I–VIII). Domain analysis of their predicted proteins showed that all contained at least one catalytic domain. However, only seven (AgCht4, AgCht5-1, AgCht6, AgCht7, AgCht8, AgCht10 and AgCht23) displayed one or more chitin-binding domains. Analyses of stage- and tissue-specific gene expression revealed that most of these genes were expressed in larval stages. However, AgCht8 was mainly expressed in the pupal and adult stages. AgCht2 and AgCht12 were specifically expressed in the foregut, whereas AgCht13 was only expressed in the midgut. The high diversity and complexity of An. gambiae chitinase and chitinase-like genes suggest their diverse functions during different developmental stages and in different tissues of the insect. A comparative genomic analysis of these genes along with those present in Drosophila melanogaster, Tribolium castaneum and several other insect species led to a uniform classification and nomenclature of these genes. Our investigation also provided important information for conducting future studies on the functions of chitinase and chitinase-like genes in this important malaria vector and other species of arthropods
Performance of the CMS Cathode Strip Chambers with Cosmic Rays
- Author
- Abadjiev K
- Abbaneo D
- Abbiendi G
- Abbrescia M
- Abdullin S
- Abelev B
- Acosta D
- Acosta JG
- Acosta MV
- Actis O
- Adam N
- Adam W
- Adams MR
- Adams T
- Adiguzel A
- Adler V
- Adolphi R
- Adzic P
- Afaq MA
- Agostino L
- Agram JL
- Aguilar-Benitez M
- Ahmad M
- Ahmad WH
- Ahmed I
- Ahmed W
- Ahuja S
- Aisa D
- Aisa S
- Akchurin N
- Akgun B
- Akgun U
- Akimenko S
- Akin IV
- Alagoz E
- Alampi G
- Albajar C
- Albayrak EA
- Alberdi J
- Albergo S
- Albert E
- Albrow M
- Alexander J
- Alidra M
- Aliev T
- Allfrey P
- Almeida N
- Altenhofer G
- Altsybeev I
- Alver B
- Alverson G
- Alves GA
- Amaglobeli N
- Amapane N
- Ambroglini F
- Amsler C
- Anagnostou G
- Ananthan B
- Anastassov A
- Andelin D
- Anderson M
- Andrea J
- Andreev V
- Andreev Y
- Anghel IM
- Anguelov T
- Anh T. Vu
- Anisimov A
- Antillon E
- Antipov P
- Antonelli L
- Anttila E
- Antunes JR
- Antunovic Z
- Apanasevich L
- Apollinari G
- Apresyan A
- Arce P
- Arcidiacono R
- Arenton MW
- Arfaei H
- Argiro S
- Arisaka K
- Arneodo M
- Arnold B
- Arora S
- Artamonov A
- Asaadi J
- Asghar MI
- Ashby S
- Askew A
- Atac M
- Atramentov O
- Auffray E
- Aurisano A
- Autermann C
- Avery P
- Avetisyan A
- Avila C
- Awan MIM
- Ayan AS
- Ayhan A
- Azhgirey I
- Aziz T
- Azzi P
- Azzurri P
- Baarmand MM
- Babb J
- Babucci E
- Baccaro S
- Bacchetta N
- Bacchi W
- Bachtis M
- Baden D
- Badgett W
- Baechler J
- Baer H
- Baesso P
- Baffioni S
- Bagby L
- Bagliesi G
- Bahk SY
- Bailleux D
- Baillon P
- Bainbridge R
- Bakhshiansohi H
- Bakirci MN
- Bakken JA
- Balazs M
- Baldin B
- Ball G
- Ball H
- Ballin J
- Bally SL
- Ban Y
- Bandurin D
- Banerjee S
- Banerjee S
- Banicz K
- Bansal S
- Banzuzi K
- Barashko V
- Barbagli G
- Barberis E
- Barbone L
- Barcala JM
- Barcellan L
- Bard R
- Bargassa P
- Baringer P
- Barnes VE
- Barnett BA
- Barney D
- Barone L
- Bartalini P
- Bartoloni A
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- Battilana C
- Baty C
- Baud A
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- Bauerdick LAT
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- Bazterra VE
- Bean A
- Beauceron S
- Beaudette F
- Beaumont W
- Bechtel F
- Bedjidian M
- Bedoya CF
- Beetz CP
- Behrens U
- Bejar JC
- Belforte S
- Beliy N
- Bell KW
- Bellan P
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- Bellato M
- Bellinger JN
- Belotelov I
- Benaglia A
- Bencze G
- Bendavid J
- Bender W
- Benedetti D
- Benelli G
- Benettoni M
- Beni N
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- Benussi L
- Benvenuti AC
- Berengueres JOL
- Beretvas A
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- Bernardini J
- Bernet C
- Berntzon L
- Berretta L
- Berry D
- Berry E
- Berryhill J
- Bertani M
- Bertl W
- Bertoldi M
- Berzano U
- Besancon M
- Besson A
- Betchart B
- Betev B
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- Beuselinck R
- Bhat PC
- Bhatnagar V
- Bhattacharya S
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- Bhatti A
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- Bianco S
- Biasini M
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- Biino C
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- Cattai A
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- Chabert EC
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- Chang P
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- Chatrchyan S
- Chatterjee A
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- Chertok M
- Chetluru V
- Cheung HWK
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- Chierici R
- Chiochia V
- Chiorboli M
- Chipaux R
- Chiumarulo F
- Chlebana F
- Choi M
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- Choudhary BC
- Choudhury RK
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- Chtchipounov L
- Chuang SH
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- Chwalek T
- Cihangir S
- Cimmino A
- Cirino G
- Cittolin S
- Ciulli V
- Civinini C
- Claes DR
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- Clemente A
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- Clerbaux B
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- Cockerill DJA
- Codispoti G
- Colafranceschi S
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- Cooper SI
- Cordonnier AM
- Cortabitarte RV
- Cossutti F
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- Coughlan JA
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- Dykstra D
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- Zeuner WD
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- Ziebarth EB
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- Publication venue
- 'IOP Publishing'
- Publication date
- 01/01/2009
- Field of study
The Cathode Strip Chambers (CSCs) constitute the primary muon tracking device
in the CMS endcaps. Their performance has been evaluated using data taken
during a cosmic ray run in fall 2008. Measured noise levels are low, with the
number of noisy channels well below 1%. Coordinate resolution was measured for
all types of chambers, and fall in the range 47 microns to 243 microns. The
efficiencies for local charged track triggers, for hit and for segments
reconstruction were measured, and are above 99%. The timing resolution per
layer is approximately 5 ns
Trypanosoma cruzi Immune Response Modulation Decreases Microbiota in Rhodnius prolixus Gut and Is Crucial for Parasite Survival and Development
- Author
- A Bexfield
- A Rivero
- AH Kollien
- B Weiss
- BS McGwire
- CA Araujo
- Caroline S. Moraes
- CB Mello
- CB Pumpuni
- CRJ Chagas
- Daniele P. Castro
- DP Castro
- DP Castro
- Eloi S. Garcia
- ES Garcia
- ES Garcia
- ES Garcia
- ES Garcia
- ES Garcia
- ES Garcia
- ES Garcia
- FA Genta
- GA Vallejo
- JC Dias
- JC Dias
- Kristin Michel
- L Akman-Anderson
- Marcelo S. Gonzalez
- MM Whitten
- MMA Whitten
- MS Gonzalez
- N Boulanger
- NFS Nogueira
- Norman A. Ratcliffe
- P Ascenzi
- P Azambuja
- P Azambuja
- P Azambuja
- P Azambuja
- Patrícia Azambuja
- PJ Waniek
- R Ursic-Bedoya
- R Ursic-Bedoya
- S Eichler
- S Moncada
- S Thomas
- S Vijay
- SA Gomes
- SH Pomerantz
- Y Carton
- Y Dong
- Z Hao
- Publication venue
- Public Library of Science
- Publication date
- 01/01/2012
- Field of study
Trypanosoma cruzi in order to complete its development in the digestive tract of Rhodnius prolixus needs to overcome the immune reactions and microbiota trypanolytic activity of the gut. We demonstrate that in R. prolixus following infection with epimastigotes of Trypanosoma cruzi clone Dm28c and, in comparison with uninfected control insects, the midgut contained (i) fewer bacteria, (ii) higher parasite numbers, and (iii) reduced nitrite and nitrate production and increased phenoloxidase and antibacterial activities. In addition, in insects pre-treated with antibiotic and then infected with Dm28c, there were also reduced bacteria numbers and a higher parasite load compared with insects solely infected with parasites. Furthermore, and in contrast to insects infected with Dm28c, infection with T. cruzi Y strain resulted in a slight decreased numbers of gut bacteria but not sufficient to mediate a successful parasite infection. We conclude that infection of R. prolixus with the T. cruzi Dm28c clone modifies the host gut immune responses to decrease the microbiota population and these changes are crucial for the parasite development in the insect gut
Strongyloides venezuelensis alkaline extract for the diagnosis of human strongyloidiasis by enzyme-linked immunosorbent assay
- Author
- Baermann G
- Conway DJ
- Costa-Cruz JM
- Dreyer G
- Eleuza Rodrigues Machado
- Gam AA
- Genta RM
- Hoffmann WA
- João Batista Alves de Oliveira
- Julia Maria Costa-Cruz
- Lindo JF
- Lowry OH
- Lúcia Helena Faccioli
- Machado ER
- Maria do Rosário de Fátima Gonçalves-Pires
- Marlene Tiduko Ueta
- Neva FA
- Ritchie LS
- Rugai E
- Sato Y
- Siddiqui AA
- Uparanukraw P
- Publication venue
- 'FapUNIFESP (SciELO)'
- Publication date
- Field of study
CMS Data Processing Workflows during an Extended Cosmic Ray Run
- Author
- Abadjiev K
- Abbaneo D
- Abbiendi G
- Abbrescia M
- Abdullin S
- Abelev B
- Acosta D
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- Adiguzel A
- Adler V
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- Afaq MA
- Agostino L
- Agram JL
- Aguilar-Benitez M
- Ahmad M
- Ahmad WH
- Ahmed I
- Ahmed W
- Ahuja S
- Aisa D
- Aisa S
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- Akgun B
- Akgun U
- Akimenko S
- Akin IV
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- Albergo S
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- Aliev T
- Allfrey P
- Almeida N
- Altenhofer G
- Altsybeev I
- Alver B
- Alverson G
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- Ambroglini F
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- Apresyan A
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- Benaglia A
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- Chan M
- Chandra A
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- Chang S
- Chang YH
- Chanon N
- Chao Y
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- Chatelain JP
- Chatrchyan S
- Chatterjee A
- Chauhan S
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- Chien CY
- Chierici R
- Chiochia V
- Chiorboli M
- Chipaux R
- Chiumarulo F
- Chlebana F
- Choi M
- Choi S
- Choi Y
- Chou JP
- Choudhary BC
- Choudhury RK
- Christian G
- Christiansen T
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- Chung J
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- Churin I
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- Civinini C
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- Clemente A
- Clemente F
- Clerbaux B
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- CMS Collaboration
- Cockerill DJA
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- Publication venue
- INSTITUTE OF PHYSICS PUBLISHING
- Publication date
- 01/01/2009
- Field of study
Peer reviewe
Aligning the CMS Muon Chambers with the Muon Alignment System during an Extended Cosmic Ray Run
- Author
- Abadjiev K
- Abbaneo D
- Abbiendi G
- Abbrescia M
- Abdullin S
- Abelev B
- Acosta D
- Acosta JG
- Acosta MV
- Actis O
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- Adam W
- Adams MR
- Adams T
- Adiguzel A
- Adler V
- Adolphi R
- Adzic P
- Afaq MA
- Agostino L
- Agram JL
- Aguilar Benitez M
- Ahmad M
- Ahmad WH
- Ahmed I
- Ahmed W
- Ahuja S
- Aisa D
- Aisa S
- Akchurin N
- Akgun B
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- Akin IV
- Alagoz E
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- Albayrak EA
- Alberdi J
- Albergo S
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- Alexander J
- Alidra M
- Aliev T
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- Almeida N
- Altenhofer G
- Altsybeev I
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- Alverson G
- Alves GA
- Amaglobeli N
- Amapane N
- Ambroglini F
- Amsler C
- Anagnostou G
- Ananthan B
- Anastassov A
- Andelin D
- Anderson M
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- Andreev V
- Andreev Y
- Anghel IM
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- Anh T. Vu
- Anisimov A
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- Asghar MI
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- Publication venue
- Publication date
- 01/01/2009
- Field of study
Peer reviewe
Transverse-momentum and pseudorapidity distributions of charged hadrons in pp collisions at √s=7 TeV
- Author
- Abbaneo D
- Abbiendi G
- Abbrescia M
- Abdullin S
- Acosta D
- Acosta JG
- Acosta MV
- Actis O
- Adam N
- Adam W
- Adams MR
- Adams T
- Adiguzel A
- Adler V
- Adzic P
- Agostino L
- Agram JL
- Aguilar-Benitez M
- Ahmad M
- Ahmad WH
- Ahmed I
- Ahuja S
- Akchurin N
- Akgun B
- Akgun U
- Akin IV
- Alagoz E
- Albajar C
- Albayrak EA
- Albergo S
- Albrow M
- Alexander J
- Aliev T
- Allfrey P
- Almeida N
- Altsybeev I
- Alver B
- Alverson G
- Alves GA
- Amapane N
- Ambroglini F
- Amsler C
- Anagnostou G
- Anastassov A
- Anderson J
- Anderson M
- Andrea J
- Andreev V
- Andreev V
- Andreev Y
- Andrews W
- Anghel IM
- Antonelli L
- Antunovic Z
- Apanasevich L
- Apollinari G
- Appelt E
- Aranyi A
- Arce P
- Arcidiacono R
- Arenton MW
- Arfaei H
- Argiro S
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- Arneodo M
- Asaadi J
- Asghar MI
- Askew A
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- Atramentov O
- Attikis A
- Auffray E
- Aumeyr T
- Autermann C
- Avery P
- Avetisyan A
- Ayhan A
- Azhgirey I
- Aziz T
- Azzi P
- Azzolini V
- Azzurri P
- Baarmand MM
- Babb J
- Bacchetta N
- Bachtis M
- Baden D
- Baesso P
- Baffioni S
- Bagliesi G
- Baillon P
- Bainbridge R
- Bakhshiansohi H
- Bakirci MN
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- Yazgan E
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- Yi K
- Yilmaz Y
- Yohay R
- Yoo HD
- Yoon AS
- York A
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- Yu SS
- Yumiceva F
- Yun JC
- Zabi A
- Zablocki J
- Zaganidis N
- Zakaria M
- Zalewski P
- Zanetti M
- Zang SL
- Zarubin A
- Zatzerklyaniy A
- Zeidler C
- Zeinali M
- Zeise M
- Zennamo J
- Zeuner WD
- Zeyrek M
- Zhang J
- Zhang Z
- Zheng Y
- Zhokin A
- Zhu B
- Zhu RY
- Zhukov V
- Zhukova V
- Ziebarth EB
- Ziegler J
- Zielinski M
- Zinonos Z
- Zito G
- Zoeller MH
- Zorbilmez C
- Zumerle G
- Zuranski A
- Publication venue
- 'American Physical Society (APS)'
- Publication date
- 01/01/2010
- Field of study
This is the pre-print version of the Published Article which can be accessed from the link below.Charged-hadron transverse-momentum and pseudorapidity distributions in proton-proton collisions at √s=7 TeV are measured with the inner tracking system of the CMS detector at the LHC. The charged-hadron yield is obtained by counting the number of reconstructed hits, hit pairs, and fully reconstructed charged-particle tracks. The combination of the three methods gives a charged-particle multiplicity per unit of pseudorapidity dNch/dη||η|<0.5=5.78±0.01(stat)±0.23(syst) for non-single-diffractive events, higher than predicted by commonly used models. The relative increase in charged-particle multiplicity from √s=0.9 to 7 TeV is [66.1±1.0(stat)±4.2(syst)]%. The mean transverse momentum is measured to be 0.545±0.005(stat)±0.015(syst) GeV/c. The results are compared with similar measurements at lower energies
Dijet azimuthal decorrelations in pp collisions at √s = 7TeV
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- Xiao H
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- Yazgan E
- Yelton J
- Yetkin T
- Yi K
- Yildirim E
- Yilmaz Y
- Yohay R
- Yoo HD
- Yoon AS
- York A
- Yu I
- Yu SS
- Yumiceva F
- Yun JC
- Zabel J
- Zabi A
- Zablocki J
- Zaganidis N
- Zakaria M
- Zalewski P
- Zanetti M
- Zang J
- Zang SL
- Zarubin A
- Zatserklyaniy A
- Zeinali M
- Zeise M
- Zeuner WD
- Zeyrek M
- Zhang J
- Zhang L
- Zhang Z
- Zheng Y
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- Zhu B
- Zhu RY
- Zhukov V
- Zhukova V
- Ziebarth EB
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- Zoeller MH
- Zorbilmez C
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- Publication venue
- American Physical Society
- Publication date
- 01/01/2011
- Field of study
This is the pre-print version of the Published Article which can be accessed from the link below.Measurements of dijet azimuthal decorrelations in pp collisions at √s=7 TeV using the CMS detector at the CERN LHC are presented. The analysis is based on an inclusive dijet event sample corresponding to an integrated luminosity of 2.9 pb-1. The results are compared to predictions from perturbative QCD calculations and various Monte Carlo event generators. The dijet azimuthal distributions are found to be sensitive to initial-state gluon radiation
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