The Immunogenicity of the Tumor-Associated Antigen α-Fetoprotein Is Enhanced by a Fusion with a Transmembrane Domain

Aim. To investigate the ability of recombinant modified vaccinia virus Ankara (rMVA) vector to induce an immune response against a well-tolerated self-antigen. Methods. rMVA vectors expressing different form of α-fetoprotein (AFP) were produced and characterized. Naïve mice were vaccinated with MVA vectors expressing the AFP antigen in either a secreted, or a membrane-bound, or an intracellular form. The immune response was monitored by an IFNΓ ELISpot assay and antibody detection. Results. Vaccination with the membrane-associated form of AFP induced a stronger CD8+ T-cell response compared to the ones obtained with the MVA encoding the secreted or the intracellular forms of AFP. Moreover, the vaccination with the membrane-bound AFP elicited the production of AFP-specific antibodies. Conclusions. The AFP transmembrane form is more immunogenic. Expressing a membrane-bound form in the context of an MVA vaccination could enhance the immunogenicity of a self-antigen

The Lantern Vol. 67, No. 1, Fall 1999

• I Write Poetry • So Many Poems Start This Way... • Mantra 99 for A. Joe • Indecision • Last Night • My Body Cut Off at the Back • Brittany Sestina • The Hearth • To Jane • Attention • Chemistry • Recombination • Buried • Going Home • Bound for Happiness • Stumble • Punk Rock • Ghost Spray and a Slingshot • Distant Voices • Beyond the Gear Shift • Damn It • Poppy Hands • Waist Deep • The Lie • Paying Your Dueshttps://digitalcommons.ursinus.edu/lantern/1155/thumbnail.jp

CMS physics technical design report : Addendum on high density QCD with heavy ions

Peer reviewe

Outcomes from elective colorectal cancer surgery during the SARS-CoV-2 pandemic

This study aimed to describe the change in surgical practice and the impact of SARS-CoV-2 on mortality after surgical resection of colorectal cancer during the initial phases of the SARS-CoV-2 pandemic

Phototransformation of tetrazoline oxime ethers: photoisomerization vs. photodegradation

International audienceFungicides showing potent biological activity in green house may have poor activity in the field due to fast photodegradation. This is the case of tetrazoline oxime ethers 1 and 2, active in the Z form, on which very little is known. A comprehensive study was therefore conducted to understand the photochemical behaviour of these compounds. It could be firmly demonstrated that both photoisomerization Z ⇄ E and photodegradation occur, and that interestingly photodegradation only takes place from E forms. Quantum yields of photoisomerization lay within the range 0.38–0.48 and those of E photodegradation in the range 0.06–0.11. During the reaction, the non-fungicidal E forms become the major isomers due to their lower solar light absorptivity than Z forms. The analytical study showed that photodegradation involves the cleavage of the N–O bond and allowed the identification of photoproducts arising from the rearrangement of the iminyl and alkoxyl radicals. Moreover, the proposed mechanism of alkoxyl radical oxidation into corresponding aldehyde and acid was elucidated using a computational study. This fundamental investigation fully explains the fast loss of the fungicidal activity of tetrazoline oxime ethers 1 and 2 in the field