International Journal of Pharma and Bio Sciences RESEARCH ARTICLE PHARMACOLOGY EVALUATION OF DNA DAMAGE BY COMET AND MTT-ASSAY IN LIVER TREATED WITH POLYGONUM BISTORTA AND TANNIC ACID.

Herbal preparations have been recommended in alternative systems of medicine for the treatment of hepatic disorders. Among the various studies involved in toxic, effect of CCl4 (1.5 ml/kg, i.p.) is oxidative damage through free radical generation. Drug-induced hepatotoxicity is still a significant unresolved clinical problem as liver is the most common site of damage. Polygonum Bistorta (PB) is powerful astringent, demulcent, diuretic, febrifuge, laxative, strongly styptic and rich in tannins. No systematic study has been done on protective efficacy of (PB) and Tannic acid (TA) to treat hepatic damage. The goal of work is to evaluate the efficacy of root extract of PB (100 mg/kg) and TA (25 mg/kg, p.o.) against CCl4 induced damage in liver. The activities of comet assay and MTT assay significantly recovered the damage towards normal and also reversed the histopathological alterations in liver. Thus, it may be concluded that PB and TA can be used to reduce the hepatic damage. Thus, it may be concluded that TA more effective towards control. This article can be downloaded from www.ijpbs.ne

Effect of monothiol along with antioxidant against mercury-induced oxidative stress in rat

1037-1044Efficacy of thiol chelators viz. N-acetyl cysteine and D-penicillamine (NAC and DPA) along with nutritional supplements viz. zinc acetate, sodium selenite and magnesium sulphate (Zn, Se and Mg) in the treatment of mercury intoxication was investigated in rats. This is of particular interest since high bonding affinity between mercuric ion and the thiol group exits. The mutual antagonism of mercury and selenium is one of the strongest examples of the interaction in the trace element field. Adult rats of Sprague-Dawley strain were administered a bolus dose of dimethyl mercury (10 mg/kg) orally. A significant rise in the aspartate aminotransferase, alanine aminotransferase, serum alkaline phosphatase, lactate dehydrogenase, gamma glutamyltranspeptidase, bilirubin and creatinine were observed. Single mercury exposure also resulted in a significant increase in lipid peroxides with a concomitant decrease in reduced glutathione level in liver, kidney and brain. A decrease in the enzymatic activities of acetyl cholinesterase in different regions of the brain was observed. These parameters were restored considerably with chelating agents along with nutritional supplementation, but NAC+Se and DPA+Mg offered significant protection in comparison with other combinations

Cotherapy of Tiron and selenium against vanadium induced toxic effects in lactating rats

Background: Vanadium is an important environmental and industrial pollutant. It has a status of reproductive toxicant and is reported to cross placental barrier. Objective: The current study was performed to assess the therapeutic efficacy of Tiron and its combination with selenium against vanadium induced toxicity in lactating and suckling rats. Materials and Methods: Rats were exposed to vanadium at a dose of 7.5 mg/kg/day (p.o.) for 20 days from 0 day of post partom (p.p.). Tiron (606 mg/kg/day, i.p.) and selenium (0.5 mg/kg/day, p.o.) were administered for 5 days on 21-25 day PP. Results: Vanadium exposure decreased blood sugar level while serum transaminases and serum alkaline phosphatase showed increased values significantly (p≤0.01). Elevation in glycogen content of liver and kidney of suckling and kidney of lactating rats was found after toxicant administration. Toxicant intoxication increased the enzymatic activity of acid phosphatase in liver of suckling and lactating and kidney of suckling rats. On the contrary alkaline phosphatase and adenosine triphosphatase activities were inhibited significantly (p≤0.01) in all the organs. Lipid peroxidation was enhanced whereas glutathione was reduced significantly in liver of suckling and lactating rats (p≤0.01). Vanadium also caused histopathological lesions. Therapies of Tiron per se and Tiron along with selenium maintained almost all blood and tissue biochemical parameters towards normal. Tiron along with selenium reduced vanadium induced lesions in lactating and sucklings rats. Conclusion: Tiron along with selenium is more effective than Tiron alone against vanadium induced toxic effect on lactating and suckling rats