Growth and elemental accumulation by canola on soil amended with coal fly ash

To explore the agronomic potential of an Australian coal fly ash, we conducted two glasshouse experiments in which we measured chlorophyll fluorescence, CO2 assimilation (A), transpiration, stomacal conductance, biomass accumulation, seed yield, and elemental uptake for canola (Brassica napus) grown on soil amended with an alkaline fly ash. In Experiment 1, application of up to 25 Mg/ha of fly ash increased A and plant weight early in the season before flowering and seed yield by up to 21%. However, at larger rates of ash application A, plant growth, chlorophyll concentration, and yield were all reduced. Increases in early vigor and seed yield were associated with enhanced uptake of phosphorus (P) by the plants treated with fly ash. Fly ash application did not influence accumulation of B, Cu, Mo, or Zn in the stems at any stage of plant growth or in the seed at harvest, except Mo concentration, which was elevated in the seed. Accumulation of these elements was mostly in the leaves, where concentrations of Cu and Mo increased with any amount of ash applied while that of B occurred only with ash applied at 625 Mg/ha. In Experiment 2, fly ash applied at 500 Mg/ha and mixed into die whole 30 cm soil core was detrimental to growth and yield of canola, compared with restricting mixing to 5 or 15 cm depth. In contrast, application of ash at 250 Mg/ha with increasing depth of mixing increased A and seed yield. We concluded that fly ash applied at not more than 25 Mg/ha and mixed into the top 10 to 15 cm of soil is sufficient to obtain yield benefits. Copyright © 2008 by the American Society of Agronomy, Crop Science Society of America, and Soil Science Society of America. All rights reserved

Photosynthetic pigment concentrations, gas exchange and vegetative growth for selected monocots and dicots treated with two contrasting coal fly ashes

There is uncertainty as to the rates of coal fly ash needed for optimum physiological processes and growth. In the current study we tested the hypothesis that photosynthetic pigments concentrations and CO2 assimilation (A) are more sensitive than dry weights in plants grown on media amended with coal fly ash. We applied the Terrestrial Plant Growth Test (Guideline 208) protocols of the Organization for Economic Cooperation and Development (OECD) to monocots [barley (Hordeum vulgare) and ryegrass (Secale cereale)] and dicots [canola (Brasica napus), radish (Raphanus sativus), field peas (Pisum sativum), and lucerne (Medicago sativa)] on media amended with fly ashes derived from semi-bituminous (gray ash) or lignite (red ash) coals at rates of 0, 2.5, 5.0, 10, or 20 Mg ha-1. The red ash had higher elemental concentrations and salinity than the gray ash. Fly ash addition had no significant effect on germination by any of the six species. At moderate rates (≤ 10 Mg ha-1) both ashes increased (p < 0.05) growth rates and concentrations of chlorophylls a and b, but reduced carotenoid concentrations. Addition of either ash increased A in radish and transpiration in barley. Growth rates and final dry weights were reduced for all of the six test species when addition rates exceeded 10 Mg ha-1 for gray ash and 5 Mg ha -1 for red ash. We concluded that plant dry weights, rather than pigment concentrations and/or instantaneous rates of photosynthesis, are more consistent for assessing subsequent growth in plants supplied with fly ash. Copyright © 2009 by the American Society of Agronomy, Crop Science Society of America, and Soil Science Society of America. All rights reserved

Stark Broadening of the B III 2s-2p Lines

We present a quantum-mechanical calculation of Stark line widths from electron-ion collisions for the $2s_{1/2}-2p_{1/2,3/2}$, lambda = 2066 and 2067 A, resonance transitions in B III. The results confirm the previous quantum-mechanical R-matrix calculations but contradict recent measurements and semi-classical and some semi-empirical calculations. The differences between the calculations can be attributed to the dominance of small L partial waves in the electron-atom scattering, while the large Stark widths inferred from the measurements would be substantially reduced if allowance is made for hydrodynamic turbulence from high Reynolds number flows and the associated Doppler broadening.Comment: 21 pages, 4 figures; to be published in Phys. Rev.

Multicolour interphase cytogenetics: 24 chromosome probes, 6 colours, 4 layers

From the late 1980s onwards, the use of DNA probes to visualise sequences on individual chromosomes (fluorescent in-situ hybridisation - FISH) revolutionised the study of cytogenetics. Following single colour experiments, more fluorochromes were added, culminating in a 24 colour assay that could distinguish all human chromosomes. Interphase cytogenetics (the detection of chromosome copy number in interphase nuclei) soon followed, however 24 colour experiments are hampered for this application as mixing fluorochromes to produce secondary colours produces images that are not easily distinguishable from overlapping signals. This study reports the development and use of a novel protocol, new fast hybridising FISH probes, and a bespoke image capture system for the assessment of chromosome copy number in interphase nuclei. The multicolour probe sets can be used individually or in sequential hybridisation layers to assess ploidy of all 24 human chromosomes in the same nucleus. Applications of this technique are in the investigation of chromosome copy number and the assessment of nuclear organisation for a range of different cell types including human sperm, cancer cells and preimplantation embryos

Husky Masquerade

Award for Best Demonstration . Abstract Face detection is the process where machines identify faces within an image or visual field. Face detection is used in analyzing emotions, and even in social networking applications, such as Snapchat. The underlying mechanism of face detection is to locate key landmarks on a person’s face. The goal is to detect faces using a webcam, find the facial landmarks of the detected faces, and overlay customized images relative to the facial landmarks. Machines need to be taught to detect faces. It is crucial to teach the program to identify the different types of jawlines. The program is provided a data model which specifies different combinations of facial landmark positions. The machine is then able to compare the objects seen in its visual field to the data model. Once the machine recognizes the landmarks, it places image overlays across the visual display or mutates the visual display. These images can be stationary across the visual field, or mobile relative to the facial landmarks. The programs built for this project are capable of detecting faces using a webcam, finding the facial landmarks of the detected faces, and overlaying customized images relative to the facial landmarks. Before the programs were created, existing software, toolboxes, and libraries were surveyed. The project’s programs closely resemble Snapchat and Instagram filters. The limitation of the programs built for this project is that they have not been optimized for smartphones; the programs work only on a laptop or a smaller computer, such as the Raspberry Pi. Hence, the programs are not widely accessible to the general public

Effects of data selection on the assimilation of AIRS data

The Atmospheric InfraRed Sounder (AIRS), flying aboard NASA's Earth Observing System (EOS) Aqua satellite with the Advanced Microwave Sounding Unit-A (AMSU-A), has been providing data for use in numerical weather prediction (NWP) and data assimilation systems (DAS) for over three years. The full AIRS data set is currently not transmitted in near-real-time (NRT) to the NWP centers. Instead, data sets with reduced spatial and spectral information are produced and made available in NRT. In this paper, we evaluate the use of different channel selections and error specifications. We achieved significant positive impact from the Aqua AIRS/AMSU-A combination in both hemispheres during our experimental time period of January 2003. The best results were obtained using a set of 156 channels that did not include any in the 6.7micron water vapor band. The latter have a large influence on both temperature and humidity analyses. If observation and background errors are not properly specified, the partitioning of temperature and humidity information from these channels will not be correct, and this can lead to a degradation in forecast skill. We found that changing the specified channel errors had a significant effect on the amount of data that entered into the analysis as a result of quality control thresholds that are related to the errors. However, changing the channel errors within a relatively small window did not significantly impact forecast skill with the 155 channel set. We also examined the effects of different types of spatial data reduction on assimilated data sets and NWP forecast skill. Whether we picked the center or the warmest AIRS pixel in a 3x3 array affected the amount of data ingested by the analysis but had a negligible impact on the forecast skill

The hand of Homo naledi

A nearly complete right hand of an adult hominin was recovered from the Rising Star cave system, South Africa. Based on associated hominin material, the bones of this hand are attributed to Homo naledi. This hand reveals a long, robust thumb and derived wrist morphology that is shared with Neandertals and modern humans, and considered adaptive for intensified manual manipulation. However, the finger bones are longer and more curved than in most australopiths, indicating frequent use of the hand during life for strong grasping during locomotor climbing and suspension. These markedly curved digits in combination with an otherwise human-like wrist and palm indicate a significant degree of climbing, despite the derived nature of many aspects of the hand and other regions of the postcranial skeleton in H. naledi

An Experimental Exploration of the QCD Phase Diagram: The Search for the Critical Point and the Onset of De-confinement

The QCD phase diagram lies at the heart of what the RHIC Physics Program is all about. While RHIC has been operating very successfully at or close to its maximum energy for almost a decade, it has become clear that this collider can also be operated at lower energies down to 5 GeV without extensive upgrades. An exploration of the full region of beam energies available at the RHIC facility is imperative. The STAR detector, due to its large uniform acceptance and excellent particle identification capabilities, is uniquely positioned to carry out this program in depth and detail. The first exploratory beam energy scan (BES) run at RHIC took place in 2010 (Run 10), since several STAR upgrades, most importantly a full barrel Time of Flight detector, are now completed which add new capabilities important for the interesting physics at BES energies. In this document we discuss current proposed measurements, with estimations of the accuracy of the measurements given an assumed event count at each beam energy.Comment: 59 pages, 78 figure

Science Requirements and Conceptual Design for a Polarized Medium Energy Electron-Ion Collider at Jefferson Lab

This report presents a brief summary of the science opportunities and program of a polarized medium energy electron-ion collider at Jefferson Lab and a comprehensive description of the conceptual design of such a collider based on the CEBAF electron accelerator facility.Comment: 160 pages, ~93 figures This work was supported by the U.S. Department of Energy, Office of Nuclear Physics, under Contract No. DE-AC05-06OR23177, DE-AC02-06CH11357, DE-AC05-060R23177, and DESC0005823. The U.S. Government retains a non-exclusive, paid-up, irrevocable, world-wide license to publish or reproduce this manuscript for U.S. Government purpose

The Glial Regenerative Response to Central Nervous System Injury Is Enabled by Pros-Notch and Pros-NFκB Feedback

Organisms are structurally robust, as cells accommodate changes preserving structural integrity and function. The molecular mechanisms underlying structural robustness and plasticity are poorly understood, but can be investigated by probing how cells respond to injury. Injury to the CNS induces proliferation of enwrapping glia, leading to axonal re-enwrapment and partial functional recovery. This glial regenerative response is found across species, and may reflect a common underlying genetic mechanism. Here, we show that injury to the Drosophila larval CNS induces glial proliferation, and we uncover a gene network controlling this response. It consists of the mutual maintenance between the cell cycle inhibitor Prospero (Pros) and the cell cycle activators Notch and NFκB. Together they maintain glia in the brink of dividing, they enable glial proliferation following injury, and subsequently they exert negative feedback on cell division restoring cell cycle arrest. Pros also promotes glial differentiation, resolving vacuolization, enabling debris clearance and axonal enwrapment. Disruption of this gene network prevents repair and induces tumourigenesis. Using wound area measurements across genotypes and time-lapse recordings we show that when glial proliferation and glial differentiation are abolished, both the size of the glial wound and neuropile vacuolization increase. When glial proliferation and differentiation are enabled, glial wound size decreases and injury-induced apoptosis and vacuolization are prevented. The uncovered gene network promotes regeneration of the glial lesion and neuropile repair. In the unharmed animal, it is most likely a homeostatic mechanism for structural robustness. This gene network may be of relevance to mammalian glia to promote repair upon CNS injury or disease