Data on European seabass fed with methionine-enriched diets obtained through label free shotgun proteomics

This data article is associated with the research article "Evaluating the impact of methionine-enriched diets in the liver of European seabass through label-free shotgun proteomics". Here it is described the data obtained from proteomic analysis of 36 European seabass juveniles (3 fish x 3 replicate tanks) after 18 days of feeding with experimental diets containing four inclusion levels of methionine (Met): 0.77%, 1%, 1.36% and 1.66% Met (w/w). We analysed this dataset and compared it with that obtained during the long-term feeding period i.e., 85 days. Fish liver proteins were digested with trypsin and purified peptides were analysed by LC-MS/MS. Proteins were identified with at least two peptides at 0.1% Decoy false discovery rate (FDR). In this dataset, we present the analysis of the differential abundant proteins (DAP) with significant differences across treatments after 18 days of feeding (One-Way ANOVA, p < 0.05). Treatment's comparisons were also performed between the 18- and 85-days feeding trials through Two-Way ANOVA (p < 0.05). MS/MS raw data are available via ProteomeXChange with identifiers PXD019610 and 10.6019/PXD019610 (18-days dataset); and PXD019622 and 10.6019/PXD019622 (85-days dataset). This dataset corresponds to fish sampled after 18-days of experimental trial and is made available to support the study conducted in the afore-mentioned article, by performing the analysis during a short-term period of feeding. The data presented may be further used in other nutritional studies e.g., addressing hepatic changes mediated by Met.ALG-01-0247-FEDER-3520/ UIDB/04326/2020/ IF/00482/2014/CP1217/CT0005info:eu-repo/semantics/publishedVersio

Effects of dietary curcumin in growth performance, oxidative status and gut morphometry and function of gilthead seabream postlarvae

An imbalance in the production and detoxification of reactive oxygen species and other oxidants can disrupt a l l types of cellular compounds, and lead to a state of oxidative stress. Preventing this state is essential to improve growth and health in animal production. Therefore, the aim of this work was to assess i f the dieta r y supple-mentation of curcumin cou l d improve the antioxidant status and intestine structu r e and functionalit y of gilthead seabream postlarvae, with the global objective of improving fish growth performance and robustness. Two experimental diets supplemented with different doses of curcumin (LOW and HIGH diets), and a commercial diet were fed to quadruplicate groups of postlarvae, for 20 days. At the end of the feeding trial fish fed the supple-mented diets significantly improved their antioxidant status compared to CTRL fed fish. LOW and HIGH fed fish presented lower protein oxidative damage (P 0.05). In conclusion, dieta r y curcumin supplementation was able to enhance gilthead seabream postlarvae robustness through a modulation of the oxidative status, increasing total antioxidant capacity and decreasing protein oxidative damage. This data pro-vide evidence that curcumin can be a suitable feed additive to promote heath status and robustness of fish at early stages of development, therefore contributing for the development and sustainabilit y of marine fish hatchery production.info:eu-repo/semantics/publishedVersio

Validation of optimal levels of micronutrients in feed for Atlantic salmon

12.11.20 Feil filnavn. ellers klar for publisering. klb Source at http://hdl.handle.net/11250/2565091ASSESSFEED er et prosjekt utgått fra EU-prosjektet ARRAINA, med målsetning å validere nye anbefalinger for tilsetning av mikronæringsstoffer, særlig vannløselige vitaminer, i laksefôr. Det ble kjørt et fôingsforsøk der kontrollfôret (100 % NP) ble tilsatt mikronæringsstoffer iht. anbefalinger gitt i NRC (2011), som i stor grad bygger på forsøk med yngel av regnbueørret fra 1980-90-tallet. Forsøksfôret (Ny NP) bygget på resultatene fra ARRAINA. Det var også et kommersielt kontrollfôr (Biomar). Det var ingen signifikant effekt på vekst, men en tendens til større biomassevekst i fisk gitt Ny NP. Opptak av vitaminer og mineraler i fisken fulgte stort sett samme mønster i ASSESSFEED som i ARRAINA, noe som styrker konklusjonene når det gjelder behov fra ARRAINA. Muskelen i fisken som fikk ny NP var bløtere og hadde større celler enn i fisken som fikk 100 % NP. Denne effekten på filetkvalitet ble tolket som økt muskelcelle hypertrofi og falt sammen med endringer i muskelens innhold av noen frie aminosyrer og nitrogen-metabolitter. Noen av disse endringene er kjent som tegn på B-vitaminmangel hos mammalier.ASSESSFEED is a project with basis in the EU-funded project ARRAINA, where the aim was to validate new recommendations for supplementation of diets for Atlantic salmon with micronutrients, especially water-soluble vitamins. A feeding trial was conducted, where the control feed (100% NP) contained micronutrients according to NRC (2011) which is largely based on trials with rainbow trout from the 1980-90ies. The experimental diet (Ny NP) was based on the results from ARRAINA. A commercial control diet was also included (Biomar). There was no significant effect on growth, but a tendency of increased biomass growth with the new NP. Uptake of vitamins and minerals in the fish largely followed the results from ARRAINA, strengthening the ARRAINA requirement estimations. The muscle in fish given Ny NP was softer and had larger cells than the muscle of fish given 100% NP. The effect on fillet quality was interpreted as increased muscle cell hyprtrophy and coincided with changes in concentrations of some free amino acids and nitrogen metabolites. Some of these changes are known as indicators of vitamin B-deficiency in mammals

Validation of optimal levels of micronutrients in feed for Atlantic salmon

ASSESSFEED er et prosjekt utgått fra EU-prosjektet ARRAINA, med målsetning å validere nye anbefalinger for tilsetning av mikronæringsstoffer, særlig vannløselige vitaminer, i laksefôr. Det ble kjørt et fôingsforsøk der kontrollfôret (100 % NP) ble tilsatt mikronæringsstoffer iht. anbefalinger gitt i NRC (2011), som i stor grad bygger på forsøk med yngel av regnbueørret fra 1980-90-tallet. Forsøksfôret (Ny NP) bygget på resultatene fra ARRAINA. Det var også et kommersielt kontrollfôr (Biomar). Det var ingen signifikant effekt på vekst, men en tendens til større biomassevekst i fisk gitt Ny NP. Opptak av vitaminer og mineraler i fisken fulgte stort sett samme mønster i ASSESSFEED som i ARRAINA, noe som styrker konklusjonene når det gjelder behov fra ARRAINA. Muskelen i fisken som fikk ny NP var bløtere og hadde større celler enn i fisken som fikk 100 % NP. Denne effekten på filetkvalitet ble tolket som økt muskelcelle hypertrofi og falt sammen med endringer i muskelens innhold av noen frie aminosyrer og nitrogen-metabolitter. Noen av disse endringene er kjent som tegn på B-vitaminmangel hos mammalier.publishedVersio

Effect of varying dietary levels of LC-PUFA and vegetable oil sources on performance and fatty acids of Senegalese sole post larvae: Puzzling results suggest complete biosynthesis pathway from C18 PUFA to DHA

Lipid nutrition of marine fish larvae has focused on supplying essential fatty acids (EFA) at high levels to meet requirements for survival, growth and development. However, some deleterious effects have been reported suggesting that excessive supply of EFA might result in insufficient supply of energy substrates, particularly in species with lower EFA requirements such as Senegalese sole (Solea senegalensis). This study addressed how the balance between EFA and non-EFA (better energy sources) affects larval performance, body composition and metabolism and retention of DHA, by formulating enrichment emulsions containing two different vegetable oil sources (olive oil or soybean oil) and three DHA levels. DHA positively affected growth and survival, independent of oil source, confirming that for sole post-larvae it is advantageous to base enrichments on vegetable oils supplying higher levels of energy, and supplement these with a DHA-rich oil. In addition, body DHA levels were generally comparable considering the large differences in their dietary supply, suggesting that the previously reported ∆4 fatty acyl desaturase (fad) operates in vivo and that DHA was synthesized at physiologically significant rates through a mechanism involving transcriptional up-regulation of ∆4fad, which was significantly up-regulated in the low DHA treatments. Furthermore, data suggested that DHA biosynthesis may be regulated by an interaction between dietary n - 3 and n - 6 PUFA, as well as by levels of LC-PUFA, and this may, under certain nutritional conditions, lead to DHA production from C18 precursors. The molecular basis of putative fatty acyl ∆5 and ∆6 desaturation activities remains to be fully determined as thorough searches have found only a single (∆4) Fads2-type transcript. Therefore, further studies are required but this might represent a unique activity described within vertebrate fads

Do dietary amino acid profiles affect performance of larval gilthead seabream?

Live preys commonly used in fish larval rearing seem to be imbalanced in terms of amino acids. Manipulation of their amino acid composition is difficult, but the use of microencapsulated diets allows this manipulation. This study analysed the effect of amino acid supplementation, in order to compensate for dietary amino acid imbalances, on growth and survival of gilthead seabream (Sparus aurata) larvae. Larvae were reared until 32 days after hatching, in a closed recirculating water system (19 °C), using only live food (rotifers and Artemia). Thereafter, larvae were fed Artemia or one of the experimental microencapsulated diets: supplemented with indispensable amino acids (IAAsup diet), or supplemented with dispensable amino acids (DAAsup diet). Experiment lasted for 10 days. Dietary supplementation with indispensable amino acids resulted in A/E ratios [(each indispensable amino acid) × (total indispensable amino acids)−1 × 1000] more similar to the ones of larval seabream and in higher IAA:DAA ratios than in the DAAsup diet. Survival was similar in larvae fed the IAAsup diet (75%) or Artemia (87%), but was significantly lower in larvae fed the DAAsup diet (52%). Larvae from all treatments more than doubled their average dry weight during the experimental period. Final dry weight was similar in larvae fed both microcapsules, but these were lighter than larvae fed with Artemia. Relative growth rate (RGR) and total biomass production tended to be higher in larvae fed the IAAsup (RGR = 9% day−1) than the DAAsup diet (RGR = 7.5% day−1) and only in this last treatment these parameters were significantly lower than in larvae fed with Artemia. Therefore, dietary supplementation with indispensable amino acids resulted in a more balanced dietary amino acid profile, which significantly increased survival. Further studies introducing microdiets earlier in the development seem necessary in order to optimise growth

Plant‐based diets fed to juvenile meagre Argyrosomus regius with low methionine and taurine supplementation led to an overall reduction in fish performance and to an increase in muscle fibre recruitment

Methionine and taurine are amino acids (AA) that are usually deficient when fish meal is replaced by plant proteins. In this study, three diets were tested in juvenile meagre (initial weight: 13.4 g) for 8 weeks. The D1 diet had 0.2% methionine and 1% taurine supplementation; the D2 and D3 diets had 0.6% methionine and 1% and 2% taurine supplementation, respectively. The results showed that meagre fed the D1 diet had lower specific growth rate (2.2 to 2.5), lower feed efficiency (0.9 to 1.2) and higher food conversion rate (FCR, 1.1 to 0.8) as well as a lower activity of the alanine aminotransferase (ALAT) enzyme. Furthermore, a higher recruitment of muscle fibres (46% compared to 36%) as well as a higher fibre density was observed (1019 compared to 870 fibres mm(-2)). This study shows that meagre requires a sufficient quantity of methionine in plant-based diets to avoid a reduction in fish performance. Furthermore, taurine supplementation in the D1 diet was not able to mitigate the effects of methionine deficiency. A higher taurine supplementation did not improve meagre performance.info:eu-repo/semantics/publishedVersio

Validering av optimale mikronæringsstoff-nivå i laksefôr - ASSESSFEED

ASSESSFEED er et prosjekt utgått fra EU-prosjektet ARRAINA, med målsetning å validere nye anbefalinger for tilsetning av mikronæringsstoffer, særlig vannløselige vitaminer, i laksefôr. Det ble kjørt et fôingsforsøk der kontrollfôret (100 % NP) ble tilsatt mikronæringsstoffer iht. anbefalinger gitt i NRC (2011), som i stor grad bygger på forsøk med yngel av regnbueørret fra 1980-90-tallet. Forsøksfôret (Ny NP) bygget på resultatene fra ARRAINA. Det var også et kommersielt kontrollfôr (Biomar). Det var ingen signifikant effekt på vekst, men en tendens til større biomassevekst i fisk gitt Ny NP. Opptak av vitaminer og mineraler i fisken fulgte stort sett samme mønster i ASSESSFEED som i ARRAINA, noe som styrker konklusjonene når det gjelder behov fra ARRAINA. Muskelen i fisken som fikk ny NP var bløtere og hadde større celler enn i fisken som fikk 100 % NP. Denne effekten på filetkvalitet ble tolket som økt muskelcelle hypertrofi og falt sammen med endringer i muskelens innhold av noen frie aminosyrer og nitrogen-metabolitter. Noen av disse endringene er kjent som tegn på B-vitaminmangel hos mammalier

Dietary protein complexity modulates growth, protein utilisation and the expression of protein digestion-related genes in Senegalese sole larvae

Given its complex metamorphosis and digestive system ontogeny, Senegalese sole larvae capacity to digest and utilize dietary protein is likely to change throughout development. In the present study, we hypothesized that the manipulation of dietary protein complexity may affect Senegalese sole larvae capacity to digest, absorb and retain protein during metamorphosis, as well as the mRNA expression of genes encoding for the precursors of proteolytic enzymes of the digestive tract and the enterocyte peptide transporter PepT1, which may have further impact on somatic growth. Three diets were formulated using approximately the same practical ingredients, except for the main protein source. The Intact diet protein content was mostly based on intact plant protein where the target peptide molecular weight (MW) would be > 70 kDa. The PartH diet protein fraction was mostly based on a protein hydrolysate with a high content of 5-70 kDa peptides. The HighH diet protein fraction was mostly based on a protein hydrolysate with a high content of 5 kDa peptides. A growth trial was performed with larvae reared at 19 degrees C under a co-feeding regime from mouth opening. The transcription of pga, tryp1c, ialp, ampn and pepT1 (encoding respectively for PepsinogenA, Trypsinogen1C, Intestinal alkaline phosphatase, Aminopeptidase N and for the enterocyte peptide transporter 1) was quantified by qPCR, during the metamorphosis climax (16 DAH) and after the metamorphosis was completed (28 DAH). An in vivo method of controlled tube-feeding was used to assess the effect on the larvae capacity to utilize polypeptides with different MW (1.0 and 7.2 kDa) representing a typical peptide MW of each of the hydrolysates included in the diets. The PartH diet stimulated growth in metamorphosing larvae (16 DAH), whereas the Intact diet stimulated growth after 36 DAH. The Intact diet stimulated the larvae absorption capacity for 1.0 kDa peptides at 16 DAH, which may have contributed for enhanced growth in later stages. The PartH diet stimulated the transcription of tryp1c and pept1 at 28 DAH, which seemed to reflect on increased post-larvae capacity to retain dietary 7.2 kDa polypeptides. That may indicate a possible strategy to optimize the digestion and utilisation of the PartH dietary protein, though it did not reflect into increased growth. The Intact diet promoted the transcription of pepsinogenA, which may reflect a reduced gastrointestinal transit time, which could have enhanced the dietary nutrients assimilation, ultimately improving growth. The present results suggest that, whereas pre-metamorphic sole larvae utilize better dietary protein with a moderate degree of hydrolysis, post-metamorphic sole make a greater use of intact protein.Project EPISOLE (FCT) [PTDC/MAR/110547/2009]FCT (Portugal) [CCMAR/Multi/04326/2013 (Portugal)]MICALA - I & DT Co-Promocao I & DT Co-Promocao (Portugal) - POAlgarve 21 [13380]MICALA - I & DT Co-Promocao I & DT Co-Promocao (Portugal) - QRENMICALA - I & DT Co-Promocao I & DT Co-Promocao (Portugal) - European UnionFCT [SFRH/BD/82149/2011]FCT investigator grant - European Social Fund [IF/00482/2014/CP1217/CT0005]Operational Programme Human PotentialFoundation for Science and Technology of Portugal (FCT)info:eu-repo/semantics/publishedVersio