A survey investigating postero-anterior chest X-ray clinical technique amongst radiographers and assistant practitioners in the UK: An extended pilot study

IntroductionWhilst many technical factors for the postero-anterior (PA) chest projection are well-researched and standardised, anecdotal evidence suggests a discrepancy regarding positioning of the X-ray tube; some radiographers using a horizontal tube, and others apply an angle. Currently there is a lack of published evidence supporting the benefits of either technique.MethodsFollowing University ethical approval, an invitation e-mail containing a link to a short questionnaire and participant information sheet was sent to radiographers and assistant practitioners in Liverpool and the surrounding areas, via professional networks/research team contacts. Questions related to length of experience, highest qualification and reasoned choice of horizontal versus angled tube preference in Computed Radiography (CR) and Digital Radiography (DR) rooms. The survey was open for nine weeks, with reminders at five and eight weeks.ResultsThere were 63 respondents. Both techniques were commonplace, with a non-statistically significant preference (p = 0.439) for a horizontal tube in both DR rooms (59%, n = 37) and CR rooms (52%, n = 30). Angled technique was employed by 41% (n = 26) of participants in DR rooms and 48% (n = 28) in CR rooms. Many participants indicated 'taught', or 'protocol', influenced their approach (46% [n = 29] in DR, 38% [n = 22] in CR). 35% (n = 10) of participants using caudal angulation, identified dose optimisation as the rationale in both CR and DR rooms. Most specifically noted reduced dose to the thyroid (69% [n = 11] in CR, 73% [n = 11] in DR).ConclusionsThere is evidence of variation in practice regarding horizontal versus an angled X-ray tube but no consistent rationale for either choice.Implications for practiceThere is a need to standardise tube positioning in PA chest radiography in line with future empirical research into the dose-optimisation implications of tube angulation

Dancing Greek Antiquity in Private and Public: Isadora Duncan's Early Patronage in Paris

This paper maps Isadora Duncan’s navigation of public and private venues, audiences, and receptions of “Greek” dances from her early career in Paris. I explore Duncan's relationship with Paris’ lesbian communities and the proliferation of ancient Greek dance in both private and public venues. Through comparisons to her contemporaries I contend that Duncan was aware of her early audiences’ interest in exotic and erotic representations of antiquity, and that she realigned these aspects of her art in later writings to appeal to changing aesthetics and interpretations of antiquity

Resilience and physical and mental well-being in adults with and without HIV

Resilience has been related to improved physical and mental health, and is thought to improve with age. No studies have explored the relationship between resilience, ageing with HIV, and well-being. A cross sectional observational study performed on UK HIV positive (N = 195) and HIV negative adults (N = 130). Associations of both age and ‘time diagnosed with HIV’ with resilience (RS-14) were assessed, and the association of resilience with depression, anxiety symptoms (PHQ-9 and GAD-7), and problems with activities of daily living (ADLs) (Euroqol 5D-3L). In a multivariable model, HIV status overall was not related to resilience. However, longer time diagnosed with HIV was related to lower resilience, and older age showed a non-significant trend towards higher resilience. In adults with HIV, high resilience was related to a lower prevalence of depression, anxiety, and problems with ADLs. It may be necessary to consider resilience when exploring the well-being of adults ageing with HIV

New genetic loci link adipose and insulin biology to body fat distribution.

Body fat distribution is a heritable trait and a well-established predictor of adverse metabolic outcomes, independent of overall adiposity. To increase our understanding of the genetic basis of body fat distribution and its molecular links to cardiometabolic traits, here we conduct genome-wide association meta-analyses of traits related to waist and hip circumferences in up to 224,459 individuals. We identify 49 loci (33 new) associated with waist-to-hip ratio adjusted for body mass index (BMI), and an additional 19 loci newly associated with related waist and hip circumference measures (P < 5 × 10(-8)). In total, 20 of the 49 waist-to-hip ratio adjusted for BMI loci show significant sexual dimorphism, 19 of which display a stronger effect in women. The identified loci were enriched for genes expressed in adipose tissue and for putative regulatory elements in adipocytes. Pathway analyses implicated adipogenesis, angiogenesis, transcriptional regulation and insulin resistance as processes affecting fat distribution, providing insight into potential pathophysiological mechanisms

Dataset in support of the Southampton doctoral thesis &#39;Characterisation of Genetic Diversity within Chlamydia trachomatis in High-Risk Sexual Networks&#39;

A zip file containing trimmed whole genome sequence reads, a spreadsheet indicating MLVA-ompA genotypes of DNA extracts obtained for the genotyping survey, and example nucleotide sequence traces.</span

Pyisotopomer: A Python package for obtaining intramolecular isotope ratio differences from mass spectrometric analysis of nitrous oxide isotopocules

Rationale: Obtaining nitrous oxide isotopocule measurements with isotope ratio mass spectrometry (IRMS) involves analyzing the ion current ratios of the nitrous oxide parent ion (N2O+) as well as those of the NO+ fragment ion. The data analysis requires correcting for “scrambling” in the ion source, whereby the NO+ fragment ion obtains the outer N atom from the N2O molecule. While descriptions exist for this correction, and interlaboratory intercalibration efforts have been made, there has yet to be published a package of code for implementing isotopomer calibrations. Methods: We developed a user-friendly Python package (pyisotopomer) to determine two coefficients (γ and κ) that describe scrambling in the IRMS ion source, and then used this calibration to obtain intramolecular isotope deltas in N2O samples. Results: With two appropriate reference materials, γ and κ can be determined robustly and accurately for a given IRMS system. An additional third reference material is needed to define the zero-point of the delta scale. We show that IRMS scrambling behavior can vary with time, necessitating regular calibrations. Finally, we present an intercalibration between two IRMS laboratories, using pyisotopomer to calculate γ and κ, and to obtain intramolecular N2O isotope deltas in lake water unknowns. Conclusions: Given these considerations, we discuss how to use pyisotopomer to obtain high-quality N2O isotopocule data from IRMS systems, including the use of appropriate reference materials and frequency of calibration

High-resolution genotyping of Lymphogranuloma Venereum (LGV) strains of Chlamydia trachomatis in London using multi-locus VNTR analysis-ompA genotyping (MLVA-ompA)

Background: lymphogranuloma venereum (LGV) is caused by Chlamydia trachomatis strains with ompA genotypes L1 to L3. An LGV epidemic associated with the L2b genotype has emerged in the past few decades amongst men who have sex with men (MSM). C. trachomatis genotypes can be discriminated by outer membrane protein A gene (ompA) sequencing, however this method has limited resolution. This study employed a high-resolution genotyping method, namely, multi-locus tandem repeat (VNTR) analysis with ompA sequencing (MLVA-ompA), to assess the distribution of LGV MLVA-ompA genotypes amongst individuals attending genitourinary medicine (GUM) clinics in London.Methods: clinical specimens were collected from individuals attending eight London-based GUM clinics. Specimens that tested positive for C. trachomatis by commercial nucleic acid amplification test (NAAT) were confirmed as LGV by pmpH real-time PCR. LGV-positive DNA extracts were subsequently genotyped using MLVA-ompA.Results: two hundred and thirty DNA extracts were confirmed as LGV, and 162 (70%) yielded complete MLVA-ompA genotypes. Six LGV MLVA-ompA genotypes were identified: 1.9.2b-L2, 1.9.3b-L2b, 1.9.2b-L2b, 1.9.2b-L2b/D, 1.4a.2b-L2b, and 5.9.2b-L1. The following LGV ompA genotypes were identified (in descending order of abundance): L2, L2b, L2b/D, and L1. Eight ompA sequences with the hybrid L2b/D profile were detected. The hybrid sequence was identical to the ompA of a recombinant L2b/D strain detected in Portugal in 2017.Conclusions: the L2 ompA genotype was found to predominate in the London study population. The study detected an unusual hybrid L2b/D ompA profile that was previously reported in Portugal. We recommend further monitoring and surveillance of LGV strains within the UK population.</p