11 research outputs found
Biological species and morphological characteristics of Armillaria mellea complex in Hokkaido: A. sinapina and two new species, A. jezoensis and A. singula
Molar tooth structures of the Neoarchean Monteville Formation, Transvaal Supergroup, South Africa. II: A wave-induced fluid flow model
Effect of Lipopolysaccharide Administration on the Number, Phenotype and Content of Nuclear Molecules in Blood Microparticles of Normal Human Subjects
Genome-Wide Identification and Expression Analysis of the Tubby-Like Protein Family in the Malus domestica Genome
Spatiotemporal immune zonation of the human kidney.
Tissue-resident immune cells are important for organ homeostasis and defense. The epithelium may contribute to these functions directly or by cross-talk with immune cells. We used single-cell RNA sequencing to resolve the spatiotemporal immune topology of the human kidney. We reveal anatomically defined expression patterns of immune genes within the epithelial compartment, with antimicrobial peptide transcripts evident in pelvic epithelium in the mature, but not fetal, kidney. A network of tissue-resident myeloid and lymphoid immune cells was evident in both fetal and mature kidney, with postnatal acquisition of transcriptional programs that promote infection-defense capabilities. Epithelial-immune cross-talk orchestrated localization of antibacterial macrophages and neutrophils to the regions of the kidney most susceptible to infection. Overall, our study provides a global overview of how the immune landscape of the human kidney is zonated to counter the dominant immunological challenge.St Baldrick's Foundation (Robert J Arceci International Award to S.B.). Additional funding was received from the Wellcome Trust (S.B.: 110104/Z/15/Z; M.H.: 107931/Z/15/Z; studentships to B.J.S., G.C., A.M.R., and C.G.). Kidney cancer bio-sampling was funded by core infrastructural funding from the Cambridge Biomedical Research Campus (CBRC) and Cancer Research UK Cambridge Centre. Additional funding in support of individual authors was provided as follows: B.J.S (CRUK predoctoral bursary, C63442/A25230); M.R.C. (CBRC; NIHR Blood and Transplant Research Unit, RG75628; MRC New Investigator Research Grant, MR/N024907/1; Arthritis Research UK Cure Challenge Research Grant, 21777; NIHR Research Professorship RP-2017-08-ST2-002); M.H. (The Lister Institute for Preventative Medicine; NIHR and Newcastle-Biomedical Research Centre); A.F. (ISAC SRL-EL program); S.Lis, S.Lin. (joint Wellcome Trust/MRC, 099175/Z/12/Z); K.W.L (Kidney Research UK Clinical Training Fellowship, TF_013_20171124). R.V-T is supported by an EMBO Long-Term Fellowship and a Human Frontier Science Progra
School-Located Influenza Vaccination With Third-Party Billing: Outcomes, Cost, and Reimbursement
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Genomic subtyping and therapeutic targeting of acute erythroleukemia.
Acute erythroid leukemia (AEL) is a high-risk leukemia of poorly understood genetic basis, with controversy regarding diagnosis in the spectrum of myelodysplasia and myeloid leukemia. We compared genomic features of 159 childhood and adult AEL cases with non-AEL myeloid disorders and defined five age-related subgroups with distinct transcriptional profiles: adult, TP53 mutated; NPM1 mutated; KMT2A mutated/rearranged; adult, DDX41 mutated; and pediatric, NUP98 rearranged. Genomic features influenced outcome, with NPM1 mutations and HOXB9 overexpression being associated with a favorable prognosis and TP53, FLT3 or RB1 alterations associated with poor survival. Targetable signaling mutations were present in 45% of cases and included recurrent mutations of ALK and NTRK1, the latter of which drives erythroid leukemogenesis sensitive to TRK inhibition. This genomic landscape of AEL provides the framework for accurate diagnosis and risk stratification of this disease, and the rationale for testing targeted therapies in this high-risk leukemia