a pore water pressure model calibration based on in situ test results

Abstract This paper proposes a procedure for the calibration of a simplified pore water pressure model implemented in 1D effective stress dynamic analyses. The calibration procedure is based on the cyclic strength of the soils as quantified using empirical correlations with in-situ tests, CPT and SPT. Specific relationships have been directly defined among the parameters of the pore water pressure model and the results of in-situ tests. All the steps for the definition of these relationships are described in detail. The proposed calibration procedure has been used to simulate the seismic response of two well-documented reclaimed sites where widespread liquefaction occurred: Port Island, in Kobe (Japan) and Treasure Island in California (US), struck by the 1995 Kobe and the 1989 Loma Prieta earthquakes, respectively. The results of the analyses have been compared to the actual site response as recorded by downhole acceleration arrays, showing that the proposed approach leads to a good estimate of the site response. Guidelines for calibration of the proposed model are finally provided, and the advantages and limitations of its use are discussed in detail

Development and assessment of a numerical model for non-linear coupled analysis on seismic response of liquefiable soils

Widespread liquefaction evidences characterized the most recent earthquakes in Italy (Emilia, 2011) and in the world (Canterbury, New Zealand, 2010-2011), resulting in a renewed interest on the characterisation and modelling of pore pressure build-up during seismic events. In particular, it has been highlighted the roughness of some traditional tools for assessing the liquefaction susceptibility as well as the overall complexity of the available numerical models. Therefore, it seems necessary to develop reliable analysis method that comprehensively take into account for the complexity of the mechanical behaviour of soils, even though remaining accessible for professional practice. In this work, a simplified constitutive model, incorporated in a one-dimensional code, is proposed. It allows to pursuing a simplified dynamic analysis: it is easy to be applied as the semi-empirical methods and reliable as an advanced numerical approach. The proposed model is based on the results of laboratory undrained cyclic tests. lt is easy to calibrate and reasonably approximates the main features of soil behaviour. The numerical formulation of the model has been incorporated in a one-dimensional computer program for seismic soil response, in order to develop a useful tool for engineering practice. In the same code it has been also implemented a numerical routine modelling the pore pressure dissipation thus allowing to simulate both the pore pressure build-up during the seismic event as well as its dissipation. The upgraded code has been validated using test sites data in order to provide an extensive documentation of the capabilities and limitations of the implemented model in prediction the seismic site response under strong ground motions. Finally, the developed code has been used to perform specific analyses on a dyke, severely damaged by the 2012 Emilia earthquake sequence. Taking into account the aftershocks that followed the main seismic event, effective stress analyses have been allowed a better understanding of the damage source detected along the embankment

Genetic and molecular characterization of the human osteosarcoma 3AB-OS cancer stem cell line: a possible model for studying osteosarcoma origin and stemness.

Finding new treatments targeting cancer stem cells (CSCs) within a tumor seems to be critical to halt cancer and improve patient survival. Osteosarcoma is an aggressive tumor affecting adolescents, for which there is no second-line chemotherapy. Uncovering new molecular mechanisms underlying the development of osteosarcoma and origin of CSCs is crucial to identify new possible therapeutic strategies. Here, we aimed to characterize genetically and molecularly the human osteosarcoma 3AB-OS CSC line, previously selected from MG63 cells and which proved to have both in vitro and in vivo features of CSCs. Classic cytogenetic studies demonstrated that 3AB-OS cells have hypertriploid karyotype with 71–82 chromosomes. By comparing 3AB-OS CSCs to the parental cells, array CGH, Affymetrix microarray, and TaqMan1 Human MicroRNA array analyses identified 49 copy number variations (CNV), 3,512 dysregulated genes and 189 differentially expressed miRNAs. Some of the chromosomal abnormalities and mRNA/miRNA expression profiles appeared to be congruent with those reported in human osteosarcomas. Bioinformatic analyses selected 196 genes and 46 anticorrelated miRNAs involved in carcinogenesis and stemness. For the first time, a predictive network is also described for two miRNA family (let-7/98 and miR-29a,b,c) and their anticorrelated mRNAs (MSTN, CCND2, Lin28B, MEST, HMGA2, and GHR), which may represent new biomarkers for osteosarcoma and may pave the way for the identification of new potential therapeutic targets

Site response analyses for complex geological and morphological conditions: relevant case-histories from 3rd level seismic microzonation in Central Italy

The paper presents the results of 5 case studies on complex site e ects selected within the project for the level 3 seismic microzonation of several municipalities of Central Italy dam- aged by the 2016 seismic sequence. The case studies are characterized by di erent geo- logical and morphological con gurations: Monte San Martino is located along a hill slope, Montedinove and Arquata del Tronto villages are located at ridge top whereas Capitignano and Norcia lie in correspondence of sediment- lled valleys. Peculiarities of the sites are constituted by the presence of weathered/jointed rock mass, fault zone, shear wave veloc- ity inversion, complex surface and buried morphologies. These factors make the de ni- tion of the subsoil model and the evaluation of the local response particularly complex and di cult to ascertain. For each site, after the discussion of the subsoil model, the results of site response numerical analyses are presented in terms of ampli cation factors and acceleration response spectra in selected points. The physical phenomena governing the site response have also been investigated at each site by comparing 1D and 2D numerical analyses. Implications are deduced for seismic microzonation studies in similar geological and morphological conditions.Published5741–57775T. Sismologia, geofisica e geologia per l'ingegneria sismicaJCR Journa

PRENOLIN project. Results of the validation phase at sendai site

One of the objectives of the PRENOLIN project is the assessment of uncertainties associated with non-linear simulation of 1D site effects. An international benchmark is underway to test several numerical codes, including various non-linear soil constitutive models, to compute the non-linear seismic site response. The preliminary verification phase (i.e. comparison between numerical codes on simple, idealistic cases) is now followed by the validation phase, which compares predictions of such numerical estimations with actual strong motion data recorded from well-known sites. The benchmark presently involves 21 teams and 21 different non-linear computations. Extensive site characterization was performed at three sites of the Japanese KiK-net and PARI networks. This paper focuses on SENDAI site. The first results indicate that a careful analysis of the data for the lab measurement is required. The linear site response is overestimated while the non-linear effects are underestimated in the first iteration. According to these observations, a first set of recommendations for defining the non-linear soil parameters from lab measurements is proposed. PRENOLIN is part of two larger projects: SINAPS@, funded by the ANR (French National Research Agency) and SIGMA, funded by a consortium of nuclear operators (EDF, CEA, AREVA, ENL)

Finalised dependability framework and evaluation results

The ambitious aim of CONNECT is to achieve universal interoperability between heterogeneous Networked Systems by means of on-the-fly synthesis of the CONNECTors through which they communicate. The goal of WP5 within CONNECT is to ensure that the non-functional properties required at each side of the connection going to be established are fulfilled, including dependability, performance, security and trust, or, in one overarching term, CONNECTability. To model such properties, we have introduced the CPMM meta-model which establishes the relevant concepts and their relations, and also includes a Complex Event language to express the behaviour associated with the specified properties. Along the four years of project duration, we have developed approaches for assuring CONNECTability both at synthesis time and at run-time. Within CONNECT architecture, these approaches are supported via the following enablers: the Dependability and Performance analysis Enabler, which is implemented in a modular architecture supporting stochastic verification and state-based analysis. Dependability and performance analysis also relies on approaches for incremental verification to adjust CONNECTor parameters at run-time; the Security Enabler, which implements a Security-by-Contract-with-Trust framework to guarantee the expected security policies and enforce them accordingly to the level of trust; the Trust Manager that implements a model-based approach to mediate between different trust models and ensure interoperable trust management. The enablers have been integrated within the CONNECT architecture, and in particular can interact with the CONNECT event-based monitoring enabler (GLIMPSE Enabler released within WP4) for run-time analysis and verification. To support a Model-driven approach in the interaction with the monitor, we have developed a CPMM editor and a translator from CPMM to the GLIMPSE native language (Drools). In this document that is the final deliverable from WP5 we first present the latest advances in the fourth year concerning CPMM, Dependability&Performance Analysis, Incremental Verification and Security. Then, we make an overall summary of main achievements for the whole project lifecycle. In appendix we also include some relevant articles specifically focussing on CONNECTability that have been prepared in the last period

Assessing the carcinogenic potential of low-dose exposures to chemical mixtures in the environment: the challenge ahead.

Lifestyle factors are responsible for a considerable portion of cancer incidence worldwide, but credible estimates from the World Health Organization and the International Agency for Research on Cancer (IARC) suggest that the fraction of cancers attributable to toxic environmental exposures is between 7% and 19%. To explore the hypothesis that low-dose exposures to mixtures of chemicals in the environment may be combining to contribute to environmental carcinogenesis, we reviewed 11 hallmark phenotypes of cancer, multiple priority target sites for disruption in each area and prototypical chemical disruptors for all targets, this included dose-response characterizations, evidence of low-dose effects and cross-hallmark effects for all targets and chemicals. In total, 85 examples of chemicals were reviewed for actions on key pathways/mechanisms related to carcinogenesis. Only 15% (13/85) were found to have evidence of a dose-response threshold, whereas 59% (50/85) exerted low-dose effects. No dose-response information was found for the remaining 26% (22/85). Our analysis suggests that the cumulative effects of individual (non-carcinogenic) chemicals acting on different pathways, and a variety of related systems, organs, tissues and cells could plausibly conspire to produce carcinogenic synergies. Additional basic research on carcinogenesis and research focused on low-dose effects of chemical mixtures needs to be rigorously pursued before the merits of this hypothesis can be further advanced. However, the structure of the World Health Organization International Programme on Chemical Safety 'Mode of Action' framework should be revisited as it has inherent weaknesses that are not fully aligned with our current understanding of cancer biology

Design of Approaches for Dependability and Initial Prototypes

The aim of CONNECT is to achieve universal interoperability between heterogeneous Networked Systems. For this, the non-functional properties required at each side of the connection going to be established must be fulfilled. By the one inclusive term "CONNECTability" we comprehend properties belonging to all four non-functional concerns of interest for CONNECT, namely dependability, performance, security and trust. We model such properties in conformance with a meta-model which establishes the relevant concepts and their relations. Then, building on the conceptual models proposed in the first year in Deliverable D5.1, in this document we present the approaches developed for assuring CONNECTability both at synthesis time and at runtime. The contributions include: the Dependability&Performance analysis Enabler, for which we release a modular architecture supporting stochastic verification and state-based analysis; incremental verification and event-based monitoring for runtime analysis; a model-based approach to interoperable trust management; the Security-by-Contract-with-Trust framework, which guarantees and enforces the expected trust levels and security policies

Local site effects and incremental damage of buildings during the 2016 Central Italy earthquake sequence

The Central Italy earthquake sequence initiated on 24 August 2016 with a moment magnitude M6.1 event followed by a M5.9 and a M6.5 earthquake, that caused significant damage and loss of life in the town of Amatrice and other nearby villages and hamlets. The significance of this sequence led to a major international reconnaissance effort to thoroughly examine the effects of this disaster. Specifically, this paper presents evidences of strong local site effects (i.e., amplification of seismic waves due to stratigraphic and topographic effects that leads to damage concentration in certain areas). It also examines the damage patterns observed along the entire sequence of events in association with the spatial distribution of ground motion intensity with emphasis on the clearly distinct performance of reinforced concrete and masonry structures under multiple excitations. The paper concludes with a critical assessment of past retrofit measures efficiency and a series of lessons learned as per the behavior of structures to a sequence of strong earthquake events