Intracellular Nanoparticle Dynamics Affected by Cytoskeletal Integrity

The cell interior is a crowded chemical space, which limits the diffusion of molecules and organelles within the cytoplasm, affecting the rates of chemical reactions. We provide insight into the relationship between non-specific intracellular diffusion and cytoskeletal integrity. Quantum dots entered the cell through microinjection and their spatial coordinates were captured by tracking their fluorescence signature as they diffused within the cell cytoplasm. Particle tracking revealed significant enhancement in the mobility of biocompatible quantum dots within fibrosarcoma cells versus their healthy counterparts, fibroblasts, as well as in actin destabilized fibroblasts versus untreated fibroblasts. Analyzing the displacement distributions provided insight into how the heterogeneity of the cell cytoskeleton influences intracellular particle diffusion. We demonstrate that intracellular diffusion of non-specific nanoparticles is enhanced by disrupting the actin network, which has implications for drug delivery efficacy and trafficking

New geomorphological and historical elements on morpho-evolutive trends and relative sea-level changes of naples coast in the last 6000 years

This research aims to present new data regarding the relative sea-level variations and related morpho-evolutive trends of Naples coast since the mid-Holocene, by interpreting several geomorphological and historical elements. The geomorphological analysis, which was applied to the emerged and submerged sector between Chiaia plain and Pizzofalcone promontory, took into account a dataset that is mainly composed of: measurements from direct surveys; bibliographic data from geological studies; historical sources; ancient pictures and maps; high-resolution digital terrain model (DTM) from Lidar; and, geo-acoustic and optical data from marine surveys off Castel dell’ Ovo carried out by using an USV (Unmanned Surface Vehicle). The GIS analysis of those data combined with iconographic researches allowed for reconstructing the high-resolution geomorphological map and three new palaeoenvironmental scenarios of the study area during the Holocene, deriving from the evaluation of the relative sea-level changes and vertical ground movements of volcano-tectonic origin affecting the coastal sector in the same period. In particular, three different relative sea-level stands were identified, dated around 6.5, 4.5, and 2.0 ky BP, respectively at +7, −5, and −3 m MSL, due to the precise mapping of several paleo-shore platforms that were ordered based on the altimetry and dated thanks to archaeological and geological interpretations

Innovative Technologies for Coastal Paleo-Landscape Reconstruction and Paleo-Sea Level Measuring

This paper is aimed at presenting an innovation-technology based approach for reconstructing underwater paleo-landscapes and related paleo-sea levels in coastal archaeological sites of great cultural value. Due to the low operational depth of the investigated area (between −0.5 and −5 m) an Unmanned Vessel Surface was used. It is equipped with instrumentation composed of both acoustic (Single Beam Echo Sounder and Side Scan Sonar) and optical sensors (a suite of cameras). This integrated instrumentation (data-fusion) has allowed acquiring simultaneously a large amount of georeferenced data by means of a Multi-Modal Mapping approach, in order to increase the perception and identification of targets. These innovative techniques have been tested in three different submerged archaeological areas of the Roman time located along the coasts of Campania (Southern Italy), with different operational scenarios. In detail, the port structures (pilae) of the Nisida harbour, and two fishponds located in the Underwater Archaeological Park of Baia have been investigated with the twofold purpose of recreating the ancient landscape and assess the vertical ground movements that have affected the volcanic district of Campi Flegrei in the last 2000 years

Coastal vs volcanic processes: Procida Island as a case of complex morpho-evolutive response

The island of Procida (Gulf of Naples, Italy) is a site of considerable importance from a historical, cultural, and landscape point of view. The island is located along a volcanic active area in the mid-Tyrrhenian and owes its formation to several explosive eruptions started more than 70 ka BP. Presently, the entire perimeter of the island is bordered by cliffs, often articulated in an alternation of headlands and coves, sometimes with shallow sandy beaches at the base (pocket beaches). However, the presence of different orders of submarine terraced surfaces represents a significant clue to coastal response to former sea-level stands.This paper aims at reconstructing the main coastal changes that occurred in the area during the Holocene mainly driven by the interaction between the post-glacial sea-level rise, vertical ground movements (VGMs) of volcano-tectonic origin, and wave action. The coastal sector was investigated using a multi-techniques approach including direct and indirect methods, in order to obtain a detailed geomorphological characterization of the whole study area. In particular, data from direct coastal surveys were integrated with photo-interpretation of areal and satellite photos, morphometric analysis of high-resolution DTMs from Lidar and bathymetric data, interpretation of morpho-acoustic data, archaeological, geomorphological and geological sea-level markers. The geomorphological analysis of this multidisciplinary dataset resulted in the detection of the main morphological elements interpreted as evidence of ancient seascapes. In particular, three orders of paleo-shore platforms were mapped in the underwater sector, allowing the evaluation of as many phases of relative sea-level stand, at-23.2 m,-11.2 m, and -4.2 m MSL dated at approximately 4.0, 2.0, and 1.0 ka BP. These coastal landforms were interpreted as the main consequence of a seacliff retreat, exacerbated by volcano-tectonic intermittent subsidence that favoured their poly-cyclic formation. However, the integration between the geomorphological and meteo-marine analysis allowed discriminating differentiated cliff backwearing responses between the western and eastern coastal sectors of the island, due to a prevailing occurrence of storms from the south-western sector. The proposed methodological approach focused on a multi-temporal geomorphological analysis applied to a multi-modal dataset, aims to comprehensive reconstruct the coastal response of a volcanic high rocky coast to the complex interaction between endogenous and exogenous forcing factors during the Holocene

On the influence of vertical ground movements on Late-Quaternary sea-level records. A comprehensive assessment along the mid-Tyrrhenian coast of Italy (Mediterranean Sea)

The mid-Tyrrhenian coast is the most complex volcano-tectonic sector in the Mediterranean basin. Here the vertical ground movements (VGMs) due to the interaction between extensive tectonic processes and volcano-tectonic activities have a significant impact on both past and future sea-level evolution. This area represents the youngest basin of the western Mediterranean and it hosts one of the most active volcanic districts, formed by the Campi Flegrei and Vesuvius volcanoes, which are worldwide known for the vertical ground movements accompanying their volcanic activity since the Late Pleistocene. The short-lived alternation of subsidence and uplift has induced great changes in inland areas and abrupt coastal landscape transformations since that time. This study aimed to spatially constrain the magnitude of the vertical displacements which significantly affected this coastal sector during the last millennia. We then reconstructed the multiple relative sea-level oscillations that occurred during the Late-Quaternary by developing a comprehensive WebGIS of sea-level index and limiting points (https://dist.altervista.org/seaproxy/). This includes newly produced sea-level data from recent marine surveys, which were coupled to previously available data standardized according to the recent international guidelines for RSL studies. This allowed producing a multiproxy dataset composed of depositional, erosional, biological, and archaeological sea-level markers, in this very complex Mediterranean area. The collected sea-level data were further compared with a number of geophysical predictions in order to disentangle the different components which influenced the sea-level evolution. Moreover, the tectonic rates derived from the available MIS 5e sea-level data were used to shift the LIG elevations vertically and backwards in time in order to constrain the Last Interglacial RSLs to certain levels depending on the areas. Finally, we used GIS techniques to spatially constrain the coastal sectors more vulnerable to the coupled effects of future sea-level rise and VGMs in the next decades