FALSE-NEGATIVE DENGUE CASES IN RORAIMA, BRAZIL: AN APPROACH REGARDING THE HIGH NUMBER OF NEGATIVE RESULTS BY NS1 AG KITS

Serum samples from 150 NS1-negative (Platelia ELISA) patients presumptively diagnosed with dengue were analyzed by the TaqMan probed real-time reverse transcription PCR (TaqMan qRT-PCR) method. The qRT-PCR positive samples were tested for serotype by semi-nested RT-PCR and a qualitative immunochromatographic assay for IgG and IgM. Molecular detection methods showed 33 (22%) positive samples out of 150 NS1-antigen negative samples. Of these, 72% were collected up to day 2 after the onset of symptoms, when diagnostic sensitivity of NS1-antigen test assays is significantly enhanced. Most of the cases were not characterized as secondary infection. Twenty-eight samples were successfully serotyped, 75% of which for DENV-4, 14% for DENV-2, 7% for DENV-3 and 4% for DENV-1. These findings reaffirm the hyperendemic situation of the state of Roraima and suggest a lower sensitivity of the NS1 test, mainly when DENV-4 is the predominant serotype. Health care providers should therefore be aware of samples tested negative by NS1 antigen assays, especially when clinical symptoms and other laboratory data results show evidence of dengue infection

FALSE-NEGATIVE DENGUE CASES IN RORAIMA, BRAZIL: AN APPROACH REGARDING THE HIGH NUMBER OF NEGATIVE RESULTS BY NS1 AG KITS

Amostras séricas de 150 pacientes, com diagnóstico presuntivo de dengue e resultado negativo para dengue por ELISA-NS1-Antígeno do kit Platelia™ (NS1-Ag), foram analisadas pela técnica de TaqMan Transcrição Reversa seguida da Reação em Cadeia da Polimerase em Tempo Real (qRT-PCR). As amostras positivas por qRT-PCR, foram submetidas a identificação dos sorotipos por RT-Hemi nested-PCR e a ensaio imunocromatográfico para detecção qualitativa dos anticorpos IgG e IgM. A técnica molecular apresentou como resultado 33 (22%) amostras positivas entre as 150 negativas pela detecção do NS1-Ag, destas o 72% foram coletadas até o segundo dia de início dos sintomas da doença, período de maior sensibilidade para pesquisas de NS1-Ag. A maioria dos casos não evidenciou infecção secundária. Dessas amostras, 28 foram satisfatoriamente sorotipadas sendo 75% de DENV-4, 14% de DENV-2, 7% de DENV-3 e 4% de DENV-1. Os resultados reafirmam a situação hiperendêmica do Estado de Roraima e sugerem baixa sensibilidade do NS1 test, especialmente quando o sorotipo predominante é DENV-4. Sugerimos assim, que a comunidade médica deve ser alertada no sentido de ser cautelosa com resultados de NS1-Ag negativo, principalmente quando sintomas clínicos e outros resultados laboratoriais sejam indicativos de provável infecção por dengue.Serum samples from 150 NS1-negative (Platelia ELISA) patients presumptively diagnosed with dengue were analyzed by the TaqMan probed real-time reverse transcription PCR (TaqMan qRT-PCR) method. The qRT-PCR positive samples were tested for serotype by semi-nested RT-PCR and a qualitative immunochromatographic assay for IgG and IgM. Molecular detection methods showed 33 (22%) positive samples out of 150 NS1-antigen negative samples. Of these, 72% were collected up to day 2 after the onset of symptoms, when diagnostic sensitivity of NS1-antigen test assays is significantly enhanced. Most of the cases were not characterized as secondary infection. Twenty-eight samples were successfully serotyped, 75% of which for DENV-4, 14% for DENV-2, 7% for DENV-3 and 4% for DENV-1. These findings reaffirm the hyperendemic situation of the state of Roraima and suggest a lower sensitivity of the NS1 test, mainly when DENV-4 is the predominant serotype. Health care providers should therefore be aware of samples tested negative by NS1 antigen assays, especially when clinical symptoms and other laboratory data results show evidence of dengue infection

Correction for Melo et al., Small molecule enoxacin is a cancer-specific growth inhibitor that acts by enhancing TAR RNA-binding protein 2-mediated microRNA processing

Medical sciences: correction for "Small molecule enoxacin is a cancer-specific growth inhibitor that acts by enhancing TAR RNA-binding protein 2-mediated microRNA processing," by SoniaMelo, Alberto Villanueva, Catia Moutinho, Veronica Davalos, Riccardo Spizzo, Cristina Ivan, Simona Rossi, Fernando Setien, Oriol Casanovas, Laia Simo-Riudalbas, Javier Carmona, Jordi Carrere, August Vidal, Alvaro Aytes, Sara Puertas, Santiago Ropero, Raghu Kalluri, Carlo M. Croce, George A. Calin, and Manel Esteller, which appeared in issue 11, March 15, 2011, of Proc Natl Acad Sci USA (108:4394-4399; first published February 28, 2011; 10.1073/pnas.1014720108)

Reemergence of Dengue Virus Serotype 3, Brazil, 2023

We characterized 3 autochthonous dengue virus serotype 3 cases and 1 imported case from 2 states in the North and South Regions of Brazil, 15 years after Brazil's last outbreak involving this serotype. We also identified a new Asian lineage recently introduced into the Americas, raising concerns about future outbreaks

Reemergence of Dengue Virus serotype 3, Brazil, 2023

Fundação Oswaldo Cruz. Rio de Janeiro, RJ, Brazil / Fundação Oswaldo Cruz. Manaus, AM, Brazil.Centers for Disease Control and Prevention. San Juan, PR, USA.Laboratório Central de Saúde Pública de Roraima. Boa Vista, RR, Brazil.Laboratório Central de Saúde Pública de Roraima. Boa Vista, RR, Brazil.Fundação Oswaldo Cruz. Manaus, AM, Brazil.Fundação Oswaldo Cruz. Manaus, AM, Brazil.Fundação Oswaldo Cruz. Manaus, AM, Brazil.Fundação Oswaldo Cruz. Manaus, AM, Brazil.Fundação Oswaldo Cruz. Manaus, AM, Brazil.Fundação de Vigilância em Saúde Dra. Rosemary Costa Pinto. Manaus, AM, Brazil.Fundação de Medicina Tropical Dr Heitor Vieira Dourado. Manaus, AM, Brazil.Ministério da Saúde. Secretaria de Vigilância em Saúde e Ambiente. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde e Ambiente. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Laboratório Central de Saúde Pública do Paraná. Curitiba, PR, Brazil.Laboratório Central de Saúde Pública do Paraná. Curitiba, PR, Brazil.Laboratório Central de Saúde Pública do Paraná. Curitiba, PR, Brazil.Fundação Oswaldo Cruz. Rio de Janeiro, RJ, Brazil.Fundação Oswaldo Cruz. Rio de Janeiro, RJ, Brazil.Fundação Oswaldo Cruz. Rio de Janeiro, RJ, BrazilFundação Oswaldo Cruz. Rio de Janeiro, RJ, Brazil.Fundação Oswaldo Cruz. Rio de Janeiro, RJ, Brazil.Fundação Oswaldo Cruz. Recife, PE, Brazil.Fundação Oswaldo Cruz. Recife, PE, Brazil.Fundação Oswaldo Cruz. Curitiba, PR, Brazil.Universidade Federal do Espírito Santo. Alegre, ES, Brazil.Florida Department of Health. Tampa, FL, USA.Florida Department of Health. Tampa, FL, USA.Centers for Disease Control and Prevention. San Juan, PR, USA.Fundação Oswaldo Cruz. Rio de Janeiro, RJ, Brazil.We characterized 3 autochthonous dengue virus serotype 3 cases and 1 imported case from 2 states in the North and South Regions of Brazil, 15 years after Brazil’s last outbreak involving this serotype. We also identified a new Asian lineage recently introduced into the Americas, raising concerns about future outbreaks

Characterisation of microbial attack on archaeological bone

As part of an EU funded project to investigate the factors influencing bone preservation in the archaeological record, more than 250 bones from 41 archaeological sites in five countries spanning four climatic regions were studied for diagenetic alteration. Sites were selected to cover a range of environmental conditions and archaeological contexts. Microscopic and physical (mercury intrusion porosimetry) analyses of these bones revealed that the majority (68%) had suffered microbial attack. Furthermore, significant differences were found between animal and human bone in both the state of preservation and the type of microbial attack present. These differences in preservation might result from differences in early taphonomy of the bones. © 2003 Elsevier Science Ltd. All rights reserved