Toxicity study in blood and tumor cells of laser produced medicines for application in fabrics

Phenothiazine derivatives are non-antibiotics with antimicrobial, fungistatic and fungicidal effects. We exposed to a high energy UV laser beam phenothiazines solutions in water at 20mg/mL concentration to increase antibacterial activity of resulting mixtures. Compared to previous results obtained on bacteria, more research is needed about UV laser irradiated phenothiazines applications on cancer cell cultures to evidence possible anticancerous properties. Evaluation of the safety of the newly obtained photoproducts in view of use on humans is also needed. Due to expensive animal testing in toxicology and pressure from general public and governments to develop alternatives to in vivo testing, in vitro cell-based models are attractive for preliminary testing of new materials. Cytotoxicity screening reported here shows that laser irradiated (4h exposure time length) chlorpromazine and promazine are more efficient against some cell cultures. Interaction of laser irradiated phenothiazines with fabrics show that promethazine and chlorpromazine have improved wetting properties. Correlation of these two groups of properties shows that chlorpromazine appears to be more recommended for applications on tissues using fabrics as transport vectors. The reported results concern stability study of phenothiazines water solutions to know the time limits within which they are stable and may be used. Keywords: Culture cells; Fabrics; Hemolysis; In vitro cytotoxicity; Laser; Phenothiazines

TiO2 nanoparticles influence on rhodamine 6G droplet emission

This work aims to investigate the effect of TiO 2 nanoparticles addition on the fluorescence emission of solutions of Rhodamine 6G excited in micro-volumetric droplets. In this paper are presented the similarities and the differences of the emission spectra by modifying parameters such as TiO 2 concentration, solutions pH and laser fluence. The pumping laser source used was the second harmonic beam emitted by a pulsed ns Nd:YAG laser at 532 nm. Lasing emission is observed and it is favorised by the solution acidity and laser beam intensity. © 2018, Editura Academiei Romane. All rights reserved

TiO2 nanoparticles influence on rhodamine 6G droplet emission

This work aims to investigate the effect of TiO 2 nanoparticles addition on the fluorescence emission of solutions of Rhodamine 6G excited in micro-volumetric droplets. In this paper are presented the similarities and the differences of the emission spectra by modifying parameters such as TiO 2 concentration, solutions pH and laser fluence. The pumping laser source used was the second harmonic beam emitted by a pulsed ns Nd:YAG laser at 532 nm. Lasing emission is observed and it is favorised by the solution acidity and laser beam intensity. © 2018, Editura Academiei Romane. All rights reserved

Biological Evaluation of Products Formed from the Irradiation of Chlorpromazine with a 266 nm Laser Beam

Models that can predict consumer choices are essential technical support fordecision makers in many contexts. The focus of this thesis is to address predictionproblems in discrete choice models and to develop methods to increase the predictivepower of these models with application to car type choice. In this thesis we challengethe common practice of prediction that is using statistical inference to estimateand select the ‘best’ model and project the results to a future situation. We showthat while the inference approaches are powerful explanatory tools in validating theexisting theories, their restrictive theory-driven assumptions make them not tailormadefor predictions. We further explore how modeling considerations for inferenceand prediction are different.Different papers of this thesis present various aspects of the prediction problemand suggest approaches and solutions to each of them.In paper 1, the problem of aggregation over alternatives, and its effects on bothestimation and prediction, is discussed. The focus of paper 2 is the model selectionfor the purpose of improving the predictive power of discrete choice models. Inpaper 3, the problem of consistency when using disaggregate logit models for anaggregate prediction question is discussed, and a model combination is proposedas tool. In paper 4, an updated version of the Swedish car fleet model is appliedto assess a Bonus-Malus policy package. Finally, in the last paper, we present thereal world applications of the Swedish car fleet model where the sensitivity of logitmodels to the specification of choice set affects prediction accuracy.QC 20160115</p

Elective cancer surgery in COVID-19-free surgical pathways during the SARS-CoV-2 pandemic: An international, multicenter, comparative cohort study

PURPOSE As cancer surgery restarts after the first COVID-19 wave, health care providers urgently require data to determine where elective surgery is best performed. This study aimed to determine whether COVID-19–free surgical pathways were associated with lower postoperative pulmonary complication rates compared with hospitals with no defined pathway. PATIENTS AND METHODS This international, multicenter cohort study included patients who underwent elective surgery for 10 solid cancer types without preoperative suspicion of SARS-CoV-2. Participating hospitals included patients from local emergence of SARS-CoV-2 until April 19, 2020. At the time of surgery, hospitals were defined as having a COVID-19–free surgical pathway (complete segregation of the operating theater, critical care, and inpatient ward areas) or no defined pathway (incomplete or no segregation, areas shared with patients with COVID-19). The primary outcome was 30-day postoperative pulmonary complications (pneumonia, acute respiratory distress syndrome, unexpected ventilation). RESULTS Of 9,171 patients from 447 hospitals in 55 countries, 2,481 were operated on in COVID-19–free surgical pathways. Patients who underwent surgery within COVID-19–free surgical pathways were younger with fewer comorbidities than those in hospitals with no defined pathway but with similar proportions of major surgery. After adjustment, pulmonary complication rates were lower with COVID-19–free surgical pathways (2.2% v 4.9%; adjusted odds ratio [aOR], 0.62; 95% CI, 0.44 to 0.86). This was consistent in sensitivity analyses for low-risk patients (American Society of Anesthesiologists grade 1/2), propensity score–matched models, and patients with negative SARS-CoV-2 preoperative tests. The postoperative SARS-CoV-2 infection rate was also lower in COVID-19–free surgical pathways (2.1% v 3.6%; aOR, 0.53; 95% CI, 0.36 to 0.76). CONCLUSION Within available resources, dedicated COVID-19–free surgical pathways should be established to provide safe elective cancer surgery during current and before future SARS-CoV-2 outbreaks

Elective Cancer Surgery in COVID-19-Free Surgical Pathways During the SARS-CoV-2 Pandemic: An International, Multicenter, Comparative Cohort Study.

PURPOSE: As cancer surgery restarts after the first COVID-19 wave, health care providers urgently require data to determine where elective surgery is best performed. This study aimed to determine whether COVID-19-free surgical pathways were associated with lower postoperative pulmonary complication rates compared with hospitals with no defined pathway. PATIENTS AND METHODS: This international, multicenter cohort study included patients who underwent elective surgery for 10 solid cancer types without preoperative suspicion of SARS-CoV-2. Participating hospitals included patients from local emergence of SARS-CoV-2 until April 19, 2020. At the time of surgery, hospitals were defined as having a COVID-19-free surgical pathway (complete segregation of the operating theater, critical care, and inpatient ward areas) or no defined pathway (incomplete or no segregation, areas shared with patients with COVID-19). The primary outcome was 30-day postoperative pulmonary complications (pneumonia, acute respiratory distress syndrome, unexpected ventilation). RESULTS: Of 9,171 patients from 447 hospitals in 55 countries, 2,481 were operated on in COVID-19-free surgical pathways. Patients who underwent surgery within COVID-19-free surgical pathways were younger with fewer comorbidities than those in hospitals with no defined pathway but with similar proportions of major surgery. After adjustment, pulmonary complication rates were lower with COVID-19-free surgical pathways (2.2% v 4.9%; adjusted odds ratio [aOR], 0.62; 95% CI, 0.44 to 0.86). This was consistent in sensitivity analyses for low-risk patients (American Society of Anesthesiologists grade 1/2), propensity score-matched models, and patients with negative SARS-CoV-2 preoperative tests. The postoperative SARS-CoV-2 infection rate was also lower in COVID-19-free surgical pathways (2.1% v 3.6%; aOR, 0.53; 95% CI, 0.36 to 0.76). CONCLUSION: Within available resources, dedicated COVID-19-free surgical pathways should be established to provide safe elective cancer surgery during current and before future SARS-CoV-2 outbreaks

The 42nd Symposium Chromatographic Methods of Investigating Organic Compounds : Book of abstracts

The 42nd Symposium Chromatographic Methods of Investigating Organic Compounds : Book of abstracts. June 4-7, 2019, Szczyrk, Polan

Photophysical study of Zn phthalocyanine in binary solvent mixtures

International audiencePhotophysical properties of phthalocyanines are important in photodynamic therapy, where these compounds are proposed as photosensitizing agents. We report here some significant solvent effects on the photophysical properties of Zn phthalocyanine (ZnPc) observed in binary solvent mixture dimethyl sulfoxide/water at several ratios of cosolvents. The absorbance of ZnPc at the maximum of Q band has a sharp drop in intensity for a water mass percent in the solvent mixture larger than 40%. The same characteristic shows also the quantum yield of fluorescence. A particular result is the increase of singlet oxygen lifetime for water percentage raise up to 20% in the solvent mixture. The effects are discussed in connection with the particular solvent microenvironment, involving DMSO/water clusters formation and the strong interaction between the solute and the solvent

Optical Characterization of Ciprofloxacin Photolytic Degradation by UV-Pulsed Laser Radiation

Ciprofloxacin is one of the most prescribed antibiotics in treating bacterial infections, becoming an important pollutant of the wastewaters. Moreover, ciprofloxacin is hard to be destroyed by conventional water treatment processes; therefore, efficient treatments to destroy it are needed in water decontamination. This study offers insights into the performance of 266 nm laser beams on the photodegradation of ciprofloxacin. An Nd:YAG laser was used that emitted 266 nm at an energy of 6.5 mJ (power of 65 mW) and ciprofloxacin water solutions were irradiated up to 240 min. The irradiated solutions were investigated by UV-Vis and FTIR absorption spectroscopy, pH assay, and laser-induced fluorescence. An HPTLC densitometer was used to characterize the laser-induced fluorescence and fluorescence lifetime of photodegradation products. The UV-Vis absorption, FTIR, and laser-induced fluorescence spectra showed the degradation of ciprofloxacin. Moreover, HPTLC densitometry offered the fluorescence and fluorescence lifetime of ciprofloxacin and its three photoproducts as well as their relative quantification. From the FTIR spectra, the molecular structure of two out of three photoproducts was proposed. In conclusion, the laser irradiation method provided the efficient photodegradation of ciprofloxacin, whereas the analytical techniques offered the proper means to monitor the process and detect the obtained photoproducts