Mussel culture experiments in Ennore estuary, Chennai

The present paper gives a detailed account on experiments of mussel culture carried out by the Institute in 1996 in association with fishermen of Ennore in an estuarine environment by adopting the long-line and rack culture methods

Environmental impact assessment in the shrimp farming areas of Nagapattinam Quaid-E-Milleth district, Tamil Nadu

To assess the effects of shrimp farming on the environment and on the coastal population, a study was undertaken by a team of scientific and technical staff of CMFRl in Nagapattinam Quaid-e-Milleth district, Tamil Nadu in September, 1995. Several small and large farms and corporate farms in nine coastal places, where a number of farms are located were covered in the study. With the help of the mobile laboratory of the CMFR Institute, the team collected and aneilysed soil, water and plankton samples in the inlet and outlet of the farm areas, shrimp ponds and from the wells of the nearby villages. Estimations on hydrological parameters, nutrients and total and differential counts of bacteria in the water and soil samples were made following standard procedures

Strontium to calcium (Sr/Ca) ratio in otolith as a tool for stock discrimination of oilsardine and mackerel

Otoliths are found in the membranous labyrinth of the inner ear of teleost fish and serve mainly in balancing the organism. They are composed of calcium carbonate and are deposited rhythmically as aragonite crystals within a protein matrix. They have long been used in fish aging studies. Recently, changes in the strontium (Sr): calcium (Ca) ratios in otoliths have received increasing attention, as these provide a method of reconstructing environmental history of the fish. Strontium is a calcium analogue sharing a similar crystal ionic radius and can substitute for Ca in the aragonite lattice of otoliths

Mortality and pulmonary complications in patients undergoing surgery with perioperative sars-cov-2 infection: An international cohort study

Background The impact of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) on postoperative recovery needs to be understood to inform clinical decision making during and after the COVID-19 pandemic. This study reports 30-day mortality and pulmonary complication rates in patients with perioperative SARS-CoV-2 infection. Methods This international, multicentre, cohort study at 235 hospitals in 24 countries included all patients undergoing surgery who had SARS-CoV-2 infection confirmed within 7 days before or 30 days after surgery. The primary outcome measure was 30-day postoperative mortality and was assessed in all enrolled patients. The main secondary outcome measure was pulmonary complications, defined as pneumonia, acute respiratory distress syndrome, or unexpected postoperative ventilation. Findings This analysis includes 1128 patients who had surgery between Jan 1 and March 31, 2020, of whom 835 (740%) had emergency surgery and 280 (248%) had elective surgery. SARS-CoV-2 infection was confirmed preoperatively in 294 (261%) patients. 30-day mortality was 238% (268 of 1128). Pulmonary complications occurred in 577 (512%) of 1128 patients; 30-day mortality in these patients was 380% (219 of 577), accounting for 817% (219 of 268) of all deaths. In adjusted analyses, 30-day mortality was associated with male sex (odds ratio 175 [95% CI 128-240], p<00001), age 70 years or older versus younger than 70 years (230 [165-322], p<00001), American Society of Anesthesiologists grades 3-5 versus grades 1-2 (235 [157-353], p<00001), malignant versus benign or obstetric diagnosis (155 [101-239], p=0046), emergency versus elective surgery (167 [106-263], p=0026), and major versus minor surgery (152 [101-231], p=0047). Interpretation Postoperative pulmonary complications occur in half of patients with perioperative SARS-CoV-2 infection and are associated with high mortality. Thresholds for surgery during the COVID-19 pandemic should be higher than during normal practice, particularly in men aged 70 years and older. Consideration should be given for postponing non-urgent procedures and promoting non-operative treatment to delay or avoid the need for surgery. Funding National Institute for Health Research (NIHR), Association of Coloproctology of Great Britain and Ireland, Bowel and Cancer Research, Bowel Disease Research Foundation, Association of Upper Gastrointestinal Surgeons, British Association of Surgical Oncology, British Gynaecological Cancer Society, European Society of Coloproctology, NIHR Academy, Sarcoma UK, Vascular Society for Great Britain and Ireland, and Yorkshire Cancer Research

Mortality and pulmonary complications in patients undergoing surgery with perioperative SARS-CoV-2 infection: an international cohort study

Background: The impact of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) on postoperative recovery needs to be understood to inform clinical decision making during and after the COVID-19 pandemic. This study reports 30-day mortality and pulmonary complication rates in patients with perioperative SARS-CoV-2 infection. Methods: This international, multicentre, cohort study at 235 hospitals in 24 countries included all patients undergoing surgery who had SARS-CoV-2 infection confirmed within 7 days before or 30 days after surgery. The primary outcome measure was 30-day postoperative mortality and was assessed in all enrolled patients. The main secondary outcome measure was pulmonary complications, defined as pneumonia, acute respiratory distress syndrome, or unexpected postoperative ventilation. Findings: This analysis includes 1128 patients who had surgery between Jan 1 and March 31, 2020, of whom 835 (74·0%) had emergency surgery and 280 (24·8%) had elective surgery. SARS-CoV-2 infection was confirmed preoperatively in 294 (26·1%) patients. 30-day mortality was 23·8% (268 of 1128). Pulmonary complications occurred in 577 (51·2%) of 1128 patients; 30-day mortality in these patients was 38·0% (219 of 577), accounting for 81·7% (219 of 268) of all deaths. In adjusted analyses, 30-day mortality was associated with male sex (odds ratio 1·75 [95% CI 1·28–2·40], p\textless0·0001), age 70 years or older versus younger than 70 years (2·30 [1·65–3·22], p\textless0·0001), American Society of Anesthesiologists grades 3–5 versus grades 1–2 (2·35 [1·57–3·53], p\textless0·0001), malignant versus benign or obstetric diagnosis (1·55 [1·01–2·39], p=0·046), emergency versus elective surgery (1·67 [1·06–2·63], p=0·026), and major versus minor surgery (1·52 [1·01–2·31], p=0·047). Interpretation: Postoperative pulmonary complications occur in half of patients with perioperative SARS-CoV-2 infection and are associated with high mortality. Thresholds for surgery during the COVID-19 pandemic should be higher than during normal practice, particularly in men aged 70 years and older. Consideration should be given for postponing non-urgent procedures and promoting non-operative treatment to delay or avoid the need for surgery. Funding: National Institute for Health Research (NIHR), Association of Coloproctology of Great Britain and Ireland, Bowel and Cancer Research, Bowel Disease Research Foundation, Association of Upper Gastrointestinal Surgeons, British Association of Surgical Oncology, British Gynaecological Cancer Society, European Society of Coloproctology, NIHR Academy, Sarcoma UK, Vascular Society for Great Britain and Ireland, and Yorkshire Cancer Research

Not Available

Not AvailableThe present paper gives a detailed account on experiments of mussel culture carried out by the Institute in 1996 in association with fishermen of Ennore in an estuarine environment by adopting the long-line and rack culture methods.Not Availabl

Novel Etoposide Analogue Modulates Expression of Angiogenesis Associated microRNAs and Regulates Cell Proliferation by Targeting STAT3 in Breast Cancer.

Tumor microenvironment play role in angiogenesis and carcinogenesis. Etoposide, a known topoisomerase II inhibitor induces DNA damage resulting in cell cycle arrest. We developed a novel Etoposide analogue, Quinazolino-4β-amidopodophyllotoxin (C-10) that show better efficacy in regulating cell proliferation and angiogenesis. We evaluated its role on expression of microRNAs-15, 16, 17 and 221 and its targets Bcl-2, STAT3 and VEGF that dictate cell proliferation and angiogenesis. Docking studies clearly demonstrated the binding of Etoposide and C-10 to STAT3. We conclude that combination of Etoposide or C-10 with miR-15, 16, 17 and 221 as a new approach to induce apoptosis and control angiogenesis in breast cancer

Not Available

Not AvailableTo assess the effects of shrimp farming on the environment and on the coastal population, a study was undertaken by a team of scientific and technical staff of CMFRl in Nagapattinam Quaid-e-Milleth district, Tamil Nadu in September, 1995. Several small and large farms and corporate farms in nine coastal places, where a number of farms are located were covered in the study. With the help of the mobile laboratory of the CMFR Institute, the team collected and aneilysed soil, water and plankton samples in the inlet and outlet of the farm areas, shrimp ponds and from the wells of the nearby villages. Estimations on hydrological parameters, nutrients and total and differential counts of bacteria in the water and soil samples were made following standard procedures.Not Availabl

Combinatorial effect of C-10 and microRNAs on its target genes.

<p>(A and B) MCF-7 and MDA-MB-231 cells were transfected with microRNA-15, 16, 17 and 221 for 24 h followed by treatment with Cisplatin, Etoposide or C-10 and incubated for 24 h. Western blot analysis showing decrease in levels of Bcl-2, STAT3 and VEGF proteins. GAPDH was used as loading control in all combinations separately.</p

C-10 modulates microRNA expression and its biogenesis.

<p>(A) Endogenous microRNA expression studies in compound treated MCF-7 and MDA-MB-231 cells showing significant upregulation of miR-15 and miR-16 in Etoposide or C-10 treated cells compared to miR-17 and miR-221. (B) The C-10 compound enhanced the expression of Drosha, Dicer, TRBP and Ago-1 enzymes that involved in synthesis and processing of matured microRNAs. (C) Computational analysis of miRNA prediction shows the possible binding sites in 3’UTR of Bcl-2, STAT3 and VEGFA for each miRNA-15, 16, 17 and 221 along with miSVR scores as depicted by miRanda software.</p