Human Motion Anticipation and Recognition from RGB-D

Predicting and understanding the dynamic of human motion has many applications such as motion synthesis, augmented reality, security, education, reinforcement learning, autonomous vehicles, and many others. In this thesis, we create a novel end-to-end pipeline that can predict multiple future poses from the same input, and, in addition, can classify the entire sequence. Our focus is on the following two aspects of human motion understanding: Probabilistic human action prediction: Given a sequence of human poses as input, we sample multiple possible future poses from the same input sequence using a new GAN-based network. Human motion understanding: Given a sequence of human poses as input, we classify the actual action performed in the sequence and improve the classification performance using the presentation learned from the prediction network. We also demonstrate how to improve model training from noisy labels, using facial expression recognition as an example. More specifically, we have 10 taggers to label each input image, and compare four different approaches: majority voting, multi-label learning, probabilistic label drawing, and cross-entropy loss. We show that the traditional majority voting scheme does not perform as well as the last two approaches that fully leverage the label distribution. We shared the enhanced FER+ data set with multiple labels for each face image with the research community (https://github.com/Microsoft/FERPlus). For predicting and understanding of human motion, we propose a novel sequence-to-sequence model trained with an improved version of generative adversarial networks (GAN). Our model, which we call HP-GAN2, learns a probability density function of future human poses conditioned on previous poses. It predicts multiple sequences of possible future human poses, each from the same input sequence but seeded with a different vector z drawn from a random distribution. Moreover, to quantify the quality of the non-deterministic predictions, we simultaneously train a motion-quality-assessment model that learns the probability that a given skeleton pose sequence is a real or fake human motion. In order to classify the action performed in a video clip, we took two approaches. In the first approach, we train on a sequence of skeleton poses from scratch using random parameters initialization with the same network architecture used in the discriminator of the HP-GAN2 model. For the second approach, we use the discriminator of the HP-GAN2 network, extend it with an action classification branch, and fine tune the end-to-end model on the classification tasks, since the discriminator in HP-GAN2 learned to differentiate between fake and real human motion. So, our hypothesis is that if the discriminator network can differentiate between synthetic and real skeleton poses, then it also has learned some of the dynamics of a real human motion, and that those dynamics are useful in classification as well. We will show through multiple experiments that that is indeed the case. Therefore, our model learns to predict multiple future sequences of human poses from the same input sequence. We also show that the discriminator learns a general representation of human motion by using the learned features in an action recognition task. And we train a motion-quality-assessment network that measure the probability of a given sequence of poses are valid human poses or not. We test our model on two of the largest human pose datasets: NTURGB-D, and Human3.6M. We train on both single and multiple action types. The predictive power of our model for motion estimation is demonstrated by generating multiple plausible futures from the same input and showing the effect of each of the several loss functions in the ablation study. We also show the advantage of switching to GAN from WGAN-GP, which we used in our previous work. Furthermore, we show that it takes less than half the number of epochs to train an activity recognition network by using the features learned from the discriminator

HP-GAN: Probabilistic 3D human motion prediction via GAN

Predicting and understanding human motion dynamics has many applications, such as motion synthesis, augmented reality, security, and autonomous vehicles. Due to the recent success of generative adversarial networks (GAN), there has been much interest in probabilistic estimation and synthetic data generation using deep neural network architectures and learning algorithms. We propose a novel sequence-to-sequence model for probabilistic human motion prediction, trained with a modified version of improved Wasserstein generative adversarial networks (WGAN-GP), in which we use a custom loss function designed for human motion prediction. Our model, which we call HP-GAN, learns a probability density function of future human poses conditioned on previous poses. It predicts multiple sequences of possible future human poses, each from the same input sequence but a different vector z drawn from a random distribution. Furthermore, to quantify the quality of the non-deterministic predictions, we simultaneously train a motion-quality-assessment model that learns the probability that a given skeleton sequence is a real human motion. We test our algorithm on two of the largest skeleton datasets: NTURGB-D and Human3.6M. We train our model on both single and multiple action types. Its predictive power for long-term motion estimation is demonstrated by generating multiple plausible futures of more than 30 frames from just 10 frames of input. We show that most sequences generated from the same input have more than 50\% probabilities of being judged as a real human sequence. We will release all the code used in this paper to Github

Cdc25B cooperates with Cdc25A to induce mitosis but has a unique role in activating cyclin B1–Cdk1 at the centrosome

Cdc25 phosphatases are essential for the activation of mitotic cyclin–Cdks, but the precise roles of the three mammalian isoforms (A, B, and C) are unclear. Using RNA interference to reduce the expression of each Cdc25 isoform in HeLa and HEK293 cells, we observed that Cdc25A and -B are both needed for mitotic entry, whereas Cdc25C alone cannot induce mitosis. We found that the G2 delay caused by small interfering RNA to Cdc25A or -B was accompanied by reduced activities of both cyclin B1–Cdk1 and cyclin A–Cdk2 complexes and a delayed accumulation of cyclin B1 protein. Further, three-dimensional time-lapse microscopy and quantification of Cdk1 phosphorylation versus cyclin B1 levels in individual cells revealed that Cdc25A and -B exert specific functions in the initiation of mitosis: Cdc25A may play a role in chromatin condensation, whereas Cdc25B specifically activates cyclin B1–Cdk1 on centrosomes

Semi-supervised Multi-modal Emotion Recognition with Cross-Modal Distribution Matching

Automatic emotion recognition is an active research topic with wide range of applications. Due to the high manual annotation cost and inevitable label ambiguity, the development of emotion recognition dataset is limited in both scale and quality. Therefore, one of the key challenges is how to build effective models with limited data resource. Previous works have explored different approaches to tackle this challenge including data enhancement, transfer learning, and semi-supervised learning etc. However, the weakness of these existing approaches includes such as training instability, large performance loss during transfer, or marginal improvement. In this work, we propose a novel semi-supervised multi-modal emotion recognition model based on cross-modality distribution matching, which leverages abundant unlabeled data to enhance the model training under the assumption that the inner emotional status is consistent at the utterance level across modalities. We conduct extensive experiments to evaluate the proposed model on two benchmark datasets, IEMOCAP and MELD. The experiment results prove that the proposed semi-supervised learning model can effectively utilize unlabeled data and combine multi-modalities to boost the emotion recognition performance, which outperforms other state-of-the-art approaches under the same condition. The proposed model also achieves competitive capacity compared with existing approaches which take advantage of additional auxiliary information such as speaker and interaction context.Comment: 10 pages, 5 figures, to be published on ACM Multimedia 202

Molecular mechanisms of cell death: recommendations of the Nomenclature Committee on Cell Death 2018.

Over the past decade, the Nomenclature Committee on Cell Death (NCCD) has formulated guidelines for the definition and interpretation of cell death from morphological, biochemical, and functional perspectives. Since the field continues to expand and novel mechanisms that orchestrate multiple cell death pathways are unveiled, we propose an updated classification of cell death subroutines focusing on mechanistic and essential (as opposed to correlative and dispensable) aspects of the process. As we provide molecularly oriented definitions of terms including intrinsic apoptosis, extrinsic apoptosis, mitochondrial permeability transition (MPT)-driven necrosis, necroptosis, ferroptosis, pyroptosis, parthanatos, entotic cell death, NETotic cell death, lysosome-dependent cell death, autophagy-dependent cell death, immunogenic cell death, cellular senescence, and mitotic catastrophe, we discuss the utility of neologisms that refer to highly specialized instances of these processes. The mission of the NCCD is to provide a widely accepted nomenclature on cell death in support of the continued development of the field

Impact of opioid-free analgesia on pain severity and patient satisfaction after discharge from surgery: multispecialty, prospective cohort study in 25 countries

Background: Balancing opioid stewardship and the need for adequate analgesia following discharge after surgery is challenging. This study aimed to compare the outcomes for patients discharged with opioid versus opioid-free analgesia after common surgical procedures.Methods: This international, multicentre, prospective cohort study collected data from patients undergoing common acute and elective general surgical, urological, gynaecological, and orthopaedic procedures. The primary outcomes were patient-reported time in severe pain measured on a numerical analogue scale from 0 to 100% and patient-reported satisfaction with pain relief during the first week following discharge. Data were collected by in-hospital chart review and patient telephone interview 1 week after discharge.Results: The study recruited 4273 patients from 144 centres in 25 countries; 1311 patients (30.7%) were prescribed opioid analgesia at discharge. Patients reported being in severe pain for 10 (i.q.r. 1-30)% of the first week after discharge and rated satisfaction with analgesia as 90 (i.q.r. 80-100) of 100. After adjustment for confounders, opioid analgesia on discharge was independently associated with increased pain severity (risk ratio 1.52, 95% c.i. 1.31 to 1.76; P < 0.001) and re-presentation to healthcare providers owing to side-effects of medication (OR 2.38, 95% c.i. 1.36 to 4.17; P = 0.004), but not with satisfaction with analgesia (beta coefficient 0.92, 95% c.i. -1.52 to 3.36; P = 0.468) compared with opioid-free analgesia. Although opioid prescribing varied greatly between high-income and low- and middle-income countries, patient-reported outcomes did not.Conclusion: Opioid analgesia prescription on surgical discharge is associated with a higher risk of re-presentation owing to side-effects of medication and increased patient-reported pain, but not with changes in patient-reported satisfaction. Opioid-free discharge analgesia should be adopted routinely

Mating type switching and transcriptional silencing in Kluyveromyces lactis

To explore the similarities and differences of regulatory circuits among budding yeasts, we characterized the role of unscheduled meiotic gene expression 6 (UME6) and a novel mating type switching pathway in Kluyveromyces lactis. We found that Ume6 was required for transcriptional silencing of the cryptic mating-type loci HMLα and HMRa. Ume6 acted directly at these loci by binding to the cis-regulatory silencers. Ume6 also served as a block to polyploidy and was required for repression of three meiotic genes, independently of the Rpd3 and Sin3 corepressors. Mating type switching from MATα to MATa required the α3 protein. The α3 protein was similar to transposases of the mutator like elements (MULEs). Mutational analysis showed that the DDE-motif in α3, which is conserved in MULEs was necessary for switching. During switching α3 mobilizes from the genome in the form of a DNA circle. The sequences encompassing the α3 gene circle junctions in the MATα locus were essential for switching from MATα to MATa. Switching also required a DNA binding protein, Mating type switch 1 (Mts1), whose binding sites in MATα were important. Expression of Mts1 was repressed in MATa/MATα diploids and by nutrients, limiting switching to haploids in low nutrient conditions. In a genetic selection for strains with increased switching rates we found a mutation in the RAS1 gene. By measuring the levels of the MTS1 mRNA and switching rates in ras1, pde2 and msn2 mutant strains we show that mating type switching in K. lactis was regulated by the RAS/cAMP pathway and the transcription factor Msn2. ras1 mutants contained 20-fold higher levels of MTS1 mRNA compared to wild type whereas pde2 and msn2 expressed less MTS1 mRNA and had decreased switching rates. Furthermore we found that MTS1 contained several potential Msn2 binding sites upstream of its ORF. We suggest that these observations explain the nutrient regulation of switching.At the time of the doctoral defense, the following papers were unpublished and had a status as follows: Paper 3: Manuscript