Detecção molecular de ovinos carreadores de Leptospira em ambiente tropical

The purpose of this study was to analyze the usefulness of PCR for the detection of leptospiral carriers in sheep under tropical field conditions. Two flocks, previously reported as seroreactive (A) and seronegative (B), were selected for this study. From those, the totality of animals of each flock, urine and vaginal fluid (VF)/semen were collected for bacteriological culture and PCR, as well as serum samples for serology. Serology confirmed the previous status of the two flocks. Culture was negative for all the samples. In PCR, animals of Flock A presented 26.7% (VF), 33.3% (semen) and 38.9% (urine) of positivity. Flock B presented 40.0% (VF), 33.3% (semen) and 5.6% (urine) of positivity by PCR. In conclusion, PCR was important to identify carriers of leptospires, including animals from a seronegative flock, what reinforces the advantages of the usage of this tool for the detection of carriers in sheep as part of control programs of leptospirosis under tropical field conditions.O objetivo do presente estudo foi analisar a aplicabilidade da PCR na detecção de ovinos carreadores de Leptospira em ambiente tropical. Brevemente, dois rebanhos ovinos, previamente reportados como sororeativo (A) e soronegativo (B) foram selecionados para este estudo. Da totalidade de animais de cada rebanho, amostras de urina e fluido vaginal (FV)/sêmen foram colhidas para cultura bacteriológica e PCR. Além disso, amostras de soro foram colhidas e utilizadas na sorologia (teste da soroaglutinação microscópica). Esta técnica confirmou o estado prévio dos dois rebanhos. Nenhuma amostra pura de leptospiras foi obtida no cultivo. Já na PCR, animais do Rebanho A apresentaram 26,7% (FV), 33,3% (sêmen) e 38,9% (urina) de amostras positivas. O Rebanho B apresentou 40,0% (FV), 33,3% (sêmen) e 5,6% (urina) de positividade pela PCR. Em conclusão, a PCR foi uma importante ferramenta na identificação de carreadores de leptospiras, incluindo animais do rebanho soronegativo, o que reforça as vantagens do uso desta técnica para a detecção de ovinos portadores como parte dos programas de controle da leptospirose em ambiente tropical.

Tecnologias e estratégias de comunicação online durante a pandemia covid-19: o caso das instituições de ensino superior em Portugal

The suspension of classroom classes from March 2020, determined by the Portuguese government in response to the CoViD-19 pandemic, imposed on educational institutions an unprecedented urgency of innovative pedagogical practices facilitated by technology. Among the many challenges, teachers had to adapt and reinvent their teaching strategies and methodologies. Having additional difficulties such as the lack of access to technologies that facilitate online classes and a concern about the possible decrease in the quality of learning due to the sudden switch to emergency remote learning (ERE). This communication proposal is an integral part of a broader study, still in progress, and from which we will present the results regarding the use of technologies for synchronous and asynchronous communication, in Portuguese higher education institutions, during the period of social confinement. The main objective of the investigation was to understand how higher education professors and students experienced the digital transition to the ERE, the pedagogical practices and the communication strategies adopted during this period. The investigation used mixed methods and, in order to respond to the proposed objectives and describe the pedagogical practices implemented and the communication strategies, specific instruments were built for data collection. A questionnaire was developed, aimed at students, and interviews aimed at both students and teachers, focusing on the technological, pedagogical and emotional dimensions, as well as on the evaluation of the experience. Data collection was carried out after the first confinement, at the end of the first semester of 2020, and took place in eight Portuguese higher education institutions (four Universities and four Polytechnic Institutes). In this communication, we will present a preliminary analysis of the results obtained and that are related to the pedagogical strategies adopted and the use of technology in synchronous and asynchronous online communication. We will also discuss the impacts of remote teaching on teaching work, evaluating whether they can bring positive and/or negative consequences to the pedagogical activity.info:eu-repo/semantics/publishedVersio

Índice de abortamento em adolescentes nos últimos 5 anos no estado do Amazonas

A adolescência dentre os seus limites, segundo a Organização Mundial da Saúde, está entre os 10 a 19 anos. Nesta fase encontram-se as primeiras descobertas, sociais, emocionais e físicas. Artigos revelam que é nesse período que eles estão em busca da autodescoberta, associada a sexualidade. O Instituto Brasileiro de Geografia e Estatística (IBGE), juntamente com o Ministério da Saúde, levantaram dados que indicaram que 30% dos adolescentes já tiveram relação sexual. Mudanças constantes nesta fase da vida, geram eventos de despreparo, sem uma responsabilidade que consequentemente é gerada ao longo da vida, tendo como resultado os riscos de reprodução que uma mulher adulta teria. Os métodos anticoncepcionais acabam sendo descartados por esse grupo etário

Patient-physician discordance in assessment of adherence to inhaled controller medication: a cross-sectional analysis of two cohorts

We aimed to compare patient's and physician's ratings of inhaled medication adherence and to identify predictors of patient-physician discordance.(SFRH/BPD/115169/2016) funded by Fundação para a Ciência e Tecnologia (FCT); ERDF (European Regional Development Fund) through the operations: POCI-01-0145-FEDER-029130 ('mINSPIRERS—mHealth to measure and improve adherence to medication in chronic obstructive respiratory diseases—generalisation and evaluation of gamification, peer support and advanced image processing technologies') cofunded by the COMPETE2020 (Programa Operacional Competitividade e Internacionalização), Portugal 2020 and by Portuguese Funds through FCT (Fundação para a Ciência e a Tecnologia).info:eu-repo/semantics/publishedVersio

Abordagem geriátrica ampla na promoção de um envelhecimento ativo e saudável: componentes do modelo de intervenção AGA@4life

info:eu-repo/semantics/publishedVersio

Integrative transcriptomic and metabolomic approaches to unravel the resistance profile of Kawisari coffee against Hemileia vastatrix

29th Conference of Association for the Science and Information on Coffee, 11 Sept. - 14 Sept. 2023 Hanoi, Vietnaminfo:eu-repo/semantics/publishedVersio

Characterization of the transcriptional signatures associated with resistance and susceptibility to Hemileia vastatrix in the Kawisari coffee hybrid

Coffee leaf rust (CLR), a disease caused by the biotrophic fungus Hemileia vastatrix (Hv), is the main threat to the worldwide production of Arabica coffee. The gradual breakdown of resistance in coffee varieties in the last years has highlighted the need for novel sources of resistance to CLR. This work aimed to unveil the cellular and molecular resistance profile of the Kawisari hybrid (C. arabica x C. liberica), a genotype used as a resistance donor in Arabica breeding programs in India. This coffee genotype was inoculated with two Hv races that triggered either resistance or susceptibility. Progress of infection was monitored using light microscopy. Simultaneously, we conducted a time-course RNA-seq characterization of the transcriptional responses. The microscopic studies showed that the post-haustorial resistance of Kawisari was associated with the hypersensitive response, accumulation of phenolic-like compounds and haustorium encasement with callose. The transcriptomic analysis suggest the downregulation of host primary metabolism genes at the early onset of infection, followed later by activation of genes functionally associated with multiple plant defense responses, including salicylic acid and jasmonate hormonal signaling. Resistance was also accompanied by the differential regulation of genes associated with phenylpropanoid metabolism and lignin biosynthesis. Our results, further validated by qPCR, provide important new insight into the molecular mechanisms underpinning resistance against CLR in this coffee genotype.Foundation for Science and Technology (FCT) and FEDER funds through PORNorte under the project CoffeeRES PTDC/ASPPLA/ 29779/2017 and by FCT UNIT LEAF (UID/AGR/04129/2020).info:eu-repo/semantics/publishedVersio

Comparação das associações cetamina-meperidina-dexmedetomidina e cetamina-meperidina-xilazina nas respostas de parâmetros fisiológicos, períodos anestésicos e recuperação anestésica de macacos prego do gênero Sapajus Kerr, 1792 (Primates: Cebidae)

Este estudo avalia, comparativamente, os efeitos de dois protocolos anestésicos em macacos prego (Sapajus sp.), sobre as variáveis fisiológicas, qualidade da anestesia e recuperação anestésica1. Participaram do estudo dezesseis espécimes de macacos prego, os quais foram divididos, aleatoriamente, em dois grupos experimentais: SX (10 mg/Kg de cloridrato de cetamina + 3 mg/Kg meperidina + 0,5 mg/Kg de cloridrato de xilazina) e SD (10 mg/Kg de cloridrato de cetamina + 3 mg/Kg de meperidina + 0,005 mg/Kg de cloridrato de dexmedetomidina). Mensurou-se os parâmetros fisiológicos durante 45 minutos no trans-anestésico: frequências cardíaca (FC) e respiratória (f), temperatura corpórea interna (TR), pressão arterial sistólica (PAS) e diastólica (PAD), saturação periférica de oxihemoglobina (SpO2) e glicemia2. Para os parâmetros referidos não foi possível observar alteração significativa entre os grupos SX e SD, p> 0,05. No entanto, houve diferença considerável entre os momentos anestésicos do grupo SX para a variável (FC) e do grupo SD para as variáveis (FC, f, TR, PAS e PAD), com regressão nos valores médios ao transcorrer das mensurações. Os parâmetros glicemia e Sp02 não apresentaram alterações fisiológicas expressivas intra e intergrupos. Para os primatas monitorados, o grupo SX apresentou maior período de latência (PL) e SD maior período de recuperação anestésica (PR). Foram acompanhados no retorno anestésico até o início da ambulação normal, não apresentando diferença (p> 0,05) intergrupos para os escores de recuperação propostos3. Para dois espécimes, anestesiados com o protocolo SD, não foi possível realizar monitoração pela ausência de relaxamento muscular adequado, sialorreia e opistótono. A profundidade do plano anestésico foi satisfatória para execução de procedimentos seguros, de curta duração e não-invasivos, em quatorze indivíduos do gênero Sapajus sp., para os grupos SX e SD4

Morphological and Postural changes in the foot during pregnancy and puerperium : a longitudinal study

The aim of this study is to observe the morphological and postural changes to the foot that take place during pregnancy and the puerperium. Method: In this descriptive, observational, longitudinal study, we analysed 23 pregnant women, with particular attention to morphological and postural aspects of the foot, at three time points during and after pregnancy: in weeks 9-13 of gestation, weeks 32-35 of gestation and weeks 4-6 after delivery. The parameters considered were changes in foot length, the Foot Posture Index (FPI) and the Hernández Corvo Index, which were analysed using a pedigraph and taking into account the Body Mass Index (BMI). The same procedure was conducted in each review. Results: The statistical analyses obtained for each foot did not differ significantly between the three measurement times. A pronator-type footprint was most frequently observed during the third trimester of pregnancy; it was predominantly neutral during the postpartum period. Statistically significant differences between the measurement times were obtained in the right foot for cavus vs. neutral foot type (between the first and third trimesters and also between the first trimester and the puerperium) (in both cases, p < 0.0001). Conclusions: Foot length increases in the third trimester and returns to normal in the puerperium. According to FPI findings, the third trimester of pregnancy is characterised by pronation, while the posture returns to neutrality during the postpartum period. During pregnancy, the plantar arch flattens, and this persists during the puerperium. The incidence of cavus foot increases significantly in the third trimester and in the puerperium

Vivência em estágio extracurricular voluntário em uma estratégia de saúde da família no município de Baião – PA: relato de experiência/ Experience in voluntary extracurricular internship through a family health strategy in the city of Baião, state of Pará: experience report

As estratégias de Saúde da Família (ESF) visam reorganizar a atenção básica em saúde no Brasil, sendo a principal porta de entrada no sistema único de saúde (SUS), e desde de 2004 é composta por uma equipe multidisciplinar de saúde. Nesse contexto, destaque-se a importância deste serviço para a comunidade. Sendo, de sua importância para acadêmicos da área da saúde durante sua formação vivenciar a rotina de trabalho deste serviço. Este trabalho trata-se de um estudo descritivo de natureza relato de experiência, tendo como objetivo descrever a vivência de acadêmicos do curso de enfermagem e terapia Ocupacional da Universidade da Amazônia (UNAMA), Faculdade Paraense de ensino (FAPEN) e Universidade Federal do Pará (UFPA), durante estágio extracurricular voluntário realizado em uma ESF no município de Baião-PA, Brasil