Mesenchymal Stem/ Stromal Cells metabolomic and bioactive factors profiles: A comparative analysis on the umbilical cord and dental pulp derived Stem/ Stromal Cells secretome

Mesenchymal Stem/ Stromal Cells assume a supporting role to the intrinsic mechanisms of tissue regeneration, a feature mostly assigned to the contents of their secretome. A comparative study on the metabolomic and bioactive molecules/factors content of the secretome of Mesenchymal Stem/ Stromal Cells derived from two expanding sources: the umbilical cord stroma and the dental pulp is presented and discussed. The metabolic profile (Nuclear Magnetic Resonance Spectroscopy) evidenced some differences in the metabolite dynamics through the conditioning period, particularly on the glucose metabolism. Despite, overall similar profiles are suggested. More prominent differences are highlighted for the bioactive factors (Multiplexing Laser Bear Analysis), in which Follistatin, Growth Regulates Protein, Hepatocyte Growth Factor, Interleukin-8 and Monocyte Chemotactic Protein-1 dominate in Umbilical Cord Mesenchymal Stem/ Stromal Cells secretion, while in Dental Pulp Stem/ Stromal Cells the Vascular Endothelial Growth Factor-A and Follistatin are more evident. The distinct secretory cocktail did not result in significantly different effects on endothelial cell populations dynamics including proliferation, migration, tube formation capacity and in vivo angiogenesis, or in chemotaxis for both Mesenchymal Stem/ Stromal Cells populations.This research was supported by Programa Operacional Regional do Norte (ON.2 - O Novo Norte), QREN, FEDER with the project "iBone Therapies: Terapias inovadoras para a regeneração óssea", ref. NORTE-01-0247-FEDER-003262, and by the program COMPETE - Programa Operacional Factores de Competitividade, Projects PEst-OE/AGR/UI0211/ 2011 and PEst-C/EME/UI0285/2013 funding from FCT. This research was also supported by Programa Operacional Competitividade e Internacionalização (P2020), Fundos Europeus Estruturais e de Investimento (FEEI) and FCT with the project "BioMate - A novel bio-manufacturing system to produce bioactive scaffolds for tissue engineering" with reference PTDC/EMS-SIS/7032/ 2014 and by COMPETE 2020, from ANI - Projetos ID&T Empresas em Copromoção, Programas Operacionais POCI, by the project "insitu.Biomas - Reinvent biomanufacturing systems by using an usability approach for in situ clinic temporary implants fabrication? with the reference POCI-01-0247-FEDER-017771. This work received further financial support from the framework of QREN through Project NORTE-07-0124-FEDER-000066. The Bruker Avance III 600 HD spectrometer was purchased under the framework of QREN, through Project NORTE-07-0162-FEDER-000048 and is part of the Portuguese NMR Network created with support of FCT through Contract REDE/1517/RMN/ 2005, with funds from POCI 2010 (FEDER). Ana Rita Caseiro (SFRH/BD/101174/2014) acknowledges FCT, for financial support. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript

Tamanho da parcela e número de repetições para experimentos com frutos de mangueira em pós-colheita.

Este trabalho teve por objetivo estudar a influência do número de repetições e do tamanho da parcela na precisão de experimentos com frutos de mangueira em pós-colheita

Comportamento, adaptabilidade e estabilidade de genótipos de feijoeiro comum no Nordeste brasileiro.

Diversas linhagens avançadas e variedades comerciais dos grupos comerciais carioca, mulatinho e preto de feijoeiro comum foram valiadas em diversas localidades dos estados da Bahia, Sergipe e Alagoas, no decorrer dos anos agrícolas de 2003 e 2004, em blocos ao acaso, com três repetições, visando conhecer o comportamento, a adaptabilidade e a estabilidade desses materiais para fins de exploração comercial. No município de Simão Dias,SE, os ensaios foram realizados nos sistemas em monocultivo e consorciado com o milho. Essa região mostrou-se mais propícia ao desenvolvimento do cultivo do feijoeiro comum, mostrando as maiores produtividades de grãos. O comportamento dos genótipos de feijoeiro nos sistemas em monocultivo e consorciado com o milho foi inconsistente, na maioria dos ensaios, revelando que a seleção de genótipos para o sistema consorciado não pode ser realizado no monocultivo. Os genótipos avaliados, dos diferentes grupos omerciais, mostraram-se diferentes quanto à adaptabilidade e à estabilidade de produção. As linhagens CNFM 7958 e CNFM 10375, do grupo mulatinho, evidenciaram melhor adaptação, além de expressarem adaptabilidade ampla, consolidando-se em alternativas importantes para a agricultura regional.bitstream/item/34048/1/bp-11.pd

Comportamento, adaptabilidade e estabilidade de cultivares de feijoeiro comum no Nordeste brasileiro.

Avaliaram-se diversas variedades e linhagens avançadas de feijoeiro comum, distribuídas em três redes experimentais, no decorrer dos anos agrícolas de 2003 e 2004, no Nordeste brasileiro, em blocos ao acaso com três repetições, visando conhecer o comportamento produtivo, a adaptabilidade e a estabilidade de produção, para fins de recomendação. Os parâmetros de adaptabilidade e estabilidade foram estimados conforme Eberhart e Russell (1966). Na maioria das avaliações nos sistemas em monocultivo e consorciado com o milho, as cultivares comportaram-se diferentemente nos sistemas praticados, evidenciando que a seleção de variedades para o consórcio não pode ser baseada no monocultivo. As cultivares diferiram quanto a adaptabilidade em todos os conjuntos estudados e mostraram boa estabilidade nos ambientes considerados. As variedades BRS Supremo, BRS Pontal, BRS Valente e Pérola e a linhagem CNFR 10241 justificaram suas recomendações para os ambientes favoráveis. As variedades Rudá, BRS Marfim, e Roxo evidenciaram adaptabilidade ampla, consubstanciando-se em alternativas importantes para a agricultura regional.bitstream/item/34050/1/bp-10.pd

Physiological and biochemical responses of apple trees to irrigation water depth in a semiarid region of Brazil.

The present study evaluated the physiological and biochemical parameters of apple trees under different irrigation water depths in a semiarid region of Brazil. The experiment had a split-plot randomized block design. Irrigation levels (60, 80, 100, and 120% of the reference evapotranspiration - ET0) and two apple cultivars (Julieta and Princesa) were analysed in the experimental plots and the phenological stages (vegetative, flowering and fruiting) were analysed in the experimental subplots. The physiological characteristics evaluated included the chlorophyll index, relative water content, net photosynthesis, stomatal conductance, transpiration, internal CO2 concentration, leaf temperature and instant carboxylation efficiency. The biochemical characteristics evaluated included the leaf contents of reducing sugars, total soluble sugars, and non-reducing sugars and proteins. The best biochemical responses were observed in the Princesa cultivar under 80% ET0 during the flowering and vegetative stages and under 120% ET0 during the fruiting stage. The best physiological responses for all phenological stages were observed under irrigation levels of 120% ET0 for both cultivars. The cv. Princesa demonstrated osmotic adjustment under water stress conditions during the phenological stages evaluated

Standalone vertex finding in the ATLAS muon spectrometer

A dedicated reconstruction algorithm to find decay vertices in the ATLAS muon spectrometer is presented. The algorithm searches the region just upstream of or inside the muon spectrometer volume for multi-particle vertices that originate from the decay of particles with long decay paths. The performance of the algorithm is evaluated using both a sample of simulated Higgs boson events, in which the Higgs boson decays to long-lived neutral particles that in turn decay to bbar b final states, and pp collision data at √s = 7 TeV collected with the ATLAS detector at the LHC during 2011

Measurements of Higgs boson production and couplings in diboson final states with the ATLAS detector at the LHC

Measurements are presented of production properties and couplings of the recently discovered Higgs boson using the decays into boson pairs, H →γ γ, H → Z Z∗ →4l and H →W W∗ →lνlν. The results are based on the complete pp collision data sample recorded by the ATLAS experiment at the CERN Large Hadron Collider at centre-of-mass energies of √s = 7 TeV and √s = 8 TeV, corresponding to an integrated luminosity of about 25 fb−1. Evidence for Higgs boson production through vector-boson fusion is reported. Results of combined fits probing Higgs boson couplings to fermions and bosons, as well as anomalous contributions to loop-induced production and decay modes, are presented. All measurements are consistent with expectations for the Standard Model Higgs boson