Variation of Hg concentration and accumulation in the soil of maritime pine plantations along a coast-inland transect in SW Europe

Financiado para publicación en acceso aberto: Universidade de Vigo/CISUGClimatic conditions have been shown as a major driver of the fate of Hg in forest ecosystems at a global scale, but less is known about climatic effects at shorter scales. This study assesses whether the concentration and pools of Hg in soils collected from seventeen Pinus pinaster stands describing a coastal-inland transect in SW Europe vary along a regional climatic gradient. In each stand, samples of the organic subhorizons (OL, OF + OH) and the mineral soil (up to 40 cm) were collected and some general physico-chemical properties and total Hg (THg) were analyzed. Total Hg was significantly higher in the OF + OH than in the OL subhorizons (98 and 38 μg kg−1, respectively), favored by a greater organic matter humification in the former. In the mineral soil, mean THg values decreased with depth, ranging from 96 μg kg−1 in the 0–5 cm layers to 54 μg kg−1 in the deepest layers (30–40 cm), respectively. The average Hg pool (PHg) was 0.30 mg m−2 in the organic horizons (92% accumulated in the OF + OH subhorizons), and 27.4 mg m−2 in the mineral soil. Changes in climatic factors, mainly precipitation, along the coast-inland transect resulted in a remarkable variation of THg in the OL subhorizons, consistent with their role as the first receiver of atmospheric Hg inputs. The high precipitation rate and the occurrence of fogs in coastal areas characterized by the oceanic influence would explain the higher THg found in the uppermost soil layers of pine stands located close to the coastline. The regional climate is key to the fate of mercury in forest ecosystems by influencing the plant growth and subsequent atmospheric Hg uptake, the atmospheric Hg transference to the soil surface (wet and dry deposition and litterfall) and the dynamics that determine net Hg accumulation in the forest floor.Ministerio de Educación y Formación Profesional | Ref. FPU17/05484Ministerio de Ciencia e Innovación | Ref. PID 2021-125114OB-I00Consellería de Cultura, Educación e Universidade (Xunta de Galicia) | Ref. ED431C2021/46- GRCUniversidad de Vigo/CISU

Needle age and precipitation as drivers of Hg accumulation and deposition in coniferous forests from a southwestern European Atlantic region

Financiado para publicación en acceso aberto: Universidade de Vigo/CISUGVegetation and climate are critical in the biogeochemical cycle of Hg in forest ecosystems. The study assesses the influence of needle age and precipitation on the accumulation of Hg in needle biomass and its deposition by litterfall in thirty-one pine plantations spread throughout two biogeographical regions in SW Europe. Welldeveloped branches of Pinus pinaster were sampled and pine needles were classified according to 4 age classes (y0, y1, y2, y3). The concentration of total Hg (THg) was analyzed in the samples and Hg content in needle biomass and its deposition by litterfall were estimated. The concentration of total Hg (THg) increased with needle age ranging from 9.1 to 32.7 μg Hg kg−1 in the youngest and oldest needles, respectively. The rate of Hg uptake (HgR) three years after needle sprouting was 10.2 ± 2.3 μg Hg kg−1 yr−1, but it decreased with needle age probably due to a diminution in photosynthetic activity as needles get older. The average total Hg stored in needle biomass (HgWt) ranged from 5.6 to 87.8 mg Hg ha−1, with intermediate needle age classes (y1 and y2) accounting for 70% of the total Hg stored in the whole needle biomass. The average deposition flux of Hg through needle litterfall (HgLt) was 1.5 μg Hg m−2 yr−1, with the y2 and y3 needles contributing most to the total Hg flux. The spatial variation of THg, HgWt and HgLt decreased from coastal pine stands, characterized by an oceanic climate, to inland pine stands, a feature closely related to the dominant precipitation regime in the study area. Climatic conditions and needle age are the main factors affecting Hg accumulation in tree foliage, and should be considered for an accurate assessment of forest Hg pools at a regional scale and their potential consequences in the functioning of terrestrial ecosystems.Ministerio de Educación y Formación Profesional | Ref. FPU17/05484Xunta de Galicia | Ref. ED431C 2021/46-GRCXunta de Galicia | Ref. ED431F2018/06-EXCELENCIAXunta de Galicia | Ref. ED431C2018/0

Mercury in a birch forest in SW Europe: Deposition flux by litterfall and pools in aboveground tree biomass and soils

Financiado para publicación en acceso aberto: Universidade de Vigo/CISUGAtmospheric mercury (Hg) is largely assimilated by vegetation and subsequently transferred to the soil by litterfall, which highlights the role of forests as one of the largest global Hg sinks within terrestrial ecosystems. We assessed the pool of Hg in the aboveground biomass (leaves, wood, bark, branches and twigs), the Hg deposition flux through litterfall over two years (by sorting fallen biomass in leaves, twigs, reproductive structures and miscellaneous) and its accumulation in the soil profile in a deciduous forest dominated by Betula alba from SW Europe. The total Hg pool in the aboveground birch biomass was in the range 532–683 mg ha−1, showing the following distribution by plant tissues: well-developed leaves (171 mg ha−1) > twigs (160 mg ha−1) > bark (159 mg ha−1) > bole wood (145 mg ha−1) > fine branches (25 mg ha−1) > thick branches (24 mg ha−1) > newly sprouted leaves (20 mg ha−1). The total Hg deposition fluxes through litterfall were 15.4 and 11.7 μg m−2 yr−1 for the two years studied, with the greatest contribution coming from birch leaves (73 %). In the soil profile, the pool of Hg in the mineral soil (37.0 mg m−2) was an order of magnitude higher than in the organic horizons (1.0 mg m−2), mostly conditioned by parameters such as soil bulk density and thickness, total C andNcontents and the presence of certain Al compounds.Ministerio de Educación y Formación Profesional | Ref. FPU17/05484Xunta de Galicia | Ref. ED431C2021/46-GRCXunta de Galicia | Ref. ED431F2018/06- EXCELENCI

Reducing the environmental impact of surgery on a global scale: systematic review and co-prioritization with healthcare workers in 132 countries

Abstract Background Healthcare cannot achieve net-zero carbon without addressing operating theatres. The aim of this study was to prioritize feasible interventions to reduce the environmental impact of operating theatres. Methods This study adopted a four-phase Delphi consensus co-prioritization methodology. In phase 1, a systematic review of published interventions and global consultation of perioperative healthcare professionals were used to longlist interventions. In phase 2, iterative thematic analysis consolidated comparable interventions into a shortlist. In phase 3, the shortlist was co-prioritized based on patient and clinician views on acceptability, feasibility, and safety. In phase 4, ranked lists of interventions were presented by their relevance to high-income countries and low–middle-income countries. Results In phase 1, 43 interventions were identified, which had low uptake in practice according to 3042 professionals globally. In phase 2, a shortlist of 15 intervention domains was generated. In phase 3, interventions were deemed acceptable for more than 90 per cent of patients except for reducing general anaesthesia (84 per cent) and re-sterilization of ‘single-use’ consumables (86 per cent). In phase 4, the top three shortlisted interventions for high-income countries were: introducing recycling; reducing use of anaesthetic gases; and appropriate clinical waste processing. In phase 4, the top three shortlisted interventions for low–middle-income countries were: introducing reusable surgical devices; reducing use of consumables; and reducing the use of general anaesthesia. Conclusion This is a step toward environmentally sustainable operating environments with actionable interventions applicable to both high– and low–middle–income countries

Specific adsorption of heavy metals in soils: individual and competitive experiments

The partitioning between the soil solid phase and the soil solution determines the mobility of pollutants like heavy metals. If nonspecific sorption takes place, the reactions are easily reversible and heavy metals are released to soil solution increasing the probability of leaching through soil profile. Mobility and leaching are also favoured if other metals are in the system and competition for specific adsorption sites takes place. In this study, desorption equilibrium experiments were conducted after adsorption ones. The specific adsorption was evaluated through the amounts of the still adsorbed Cu, Pb, Cr, Ni and Zn after desorption experiments in ten different soils. In addition, competition adsorption and desorption binary experiments were conducted for evaluating the metal competition in three of the soils. Pb and Cu are the metals adsorbed and retained in higher amounts in all the studied soils. In slightly neutral soils, Cr is retained in lesser amounts while in acidic soils Zn is the metal less retained. Results showed that despite the high and variable amounts of organic matter in the soils, soil pH is the most important variable in neutral soils. In acidic soils, soil properties different than pH play important roles and specific sorption of Pb is related to the cationic exchange capacity of the soils while that of Zn to the clay content. Instead, the release of Cu during desorption experiments is probably due to the more soluble organic fraction of the soils. The individual retention of Cu, Zn, Ni and Pb is higher than when they are in competition, except if Cr is present. In this case, the amount of those four metals and that of Cr increased. Therefore, the presence of Cr together with cationic heavy metals favoured the adsorption of those metals in multi-metal polluted areas. Specific adsorption is also important during competition as soil affinities increase during competition experiments.Xunta de Galicia | Ref. ED431F 2018/06Ministerio de Economía y Competitividad | Ref. CTM2015-73422-JINXunta de Galicia | Ref. ED431E 2018/07Xunta de Galicia | Ref. ED431C 2017/62-GR

The role of afforestation species as a driver of Hg accumulation in organic horizons of forest soils from a Mediterranean mountain area in SW Europe

Financiado para publicación en acceso aberto: Universidade de Vigo/CISUGForest areas are a primary sink of atmospheric mercury (Hg) within terrestrial ecosystems, whereas forest vegetation plays a key role in atmospheric Hg transfer to soil horizons. This study assessed variations in total Hg contents (HgT) and accumulation (HgRes) in the soil organic horizons of a forest area in NE Portugal, where post-wildfire afforestation led to the substitution of the native deciduous species (Quercus pyrenaica) by fast-growing coniferous species (Pseudotsuga menziesii and Pinus nigra). The study also evaluated, for each species, the links between Hg contents and other biophilic elements of soil organic matter (C, N, S) present in organic subhorizons (OL, OF, OH). Mean HgT in the organic horizons of the different tree species follow the sequence: P. nigra (88 μg kg−1) < Q. pyrenaica (101 μg kg−1) < P. menziesii (141 μg kg−1). The highest HgRes for the entire organic horizon was found under P. menziesii (471 μg m−2), followed by P. nigra (253 μg m−2) and Q. pyrenaica (189 μg m−2). Among the organic subhorizons, values of HgT and HgRes follow the sequence OL < OF < OH, which is consistent with the degree of organic matter humification. Indeed, HgT and HgRes correlated significantly with the C/N and C/S ratios for all species and organic subhorizons, suggesting that the quality of organic matter may influence strongly the Hg fate in these forest soils. Soils from P. menziesii plots have shown an HgRes 2.5 times higher than in plots dominated by the native Q. pyrenaica. Hg accumulation in the organic horizons, promoted in the coniferous species, may increase the risk of Hg mobilization due to wildfires and forest management practices. Therefore, forest management plans should select cautiously the tree species for afforestation in order to minimize adverse environmental effects caused by changes in the biogeochemical cycle of contaminants such as Hg.Ministerio de Educación, Cultura y Deporte | Ref. FPU17/05484Xunta de Galicia | Ref. ED431C 2021/46Xunta de Galicia | Ref. ED431F2018/0

Guidelines for the use and interpretation of assays for monitoring autophagy (4th edition)

In 2008, we published the first set of guidelines for standardizing research in autophagy. Since then, this topic has received increasing attention, and many scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Thus, it is important to formulate on a regular basis updated guidelines for monitoring autophagy in different organisms. Despite numerous reviews, there continues to be confusion regarding acceptable methods to evaluate autophagy, especially in multicellular eukaryotes. Here, we present a set of guidelines for investigators to select and interpret methods to examine autophagy and related processes, and for reviewers to provide realistic and reasonable critiques of reports that are focused on these processes. These guidelines are not meant to be a dogmatic set of rules, because the appropriateness of any assay largely depends on the question being asked and the system being used. Moreover, no individual assay is perfect for every situation, calling for the use of multiple techniques to properly monitor autophagy in each experimental setting. Finally, several core components of the autophagy machinery have been implicated in distinct autophagic processes (canonical and noncanonical autophagy), implying that genetic approaches to block autophagy should rely on targeting two or more autophagy-related genes that ideally participate in distinct steps of the pathway. Along similar lines, because multiple proteins involved in autophagy also regulate other cellular pathways including apoptosis, not all of them can be used as a specific marker for bona fide autophagic responses. Here, we critically discuss current methods of assessing autophagy and the information they can, or cannot, provide. Our ultimate goal is to encourage intellectual and technical innovation in the field

Rare predicted loss-of-function variants of type I IFN immunity genes are associated with life-threatening COVID-19

BackgroundWe previously reported that impaired type I IFN activity, due to inborn errors of TLR3- and TLR7-dependent type I interferon (IFN) immunity or to autoantibodies against type I IFN, account for 15-20% of cases of life-threatening COVID-19 in unvaccinated patients. Therefore, the determinants of life-threatening COVID-19 remain to be identified in similar to 80% of cases.MethodsWe report here a genome-wide rare variant burden association analysis in 3269 unvaccinated patients with life-threatening COVID-19, and 1373 unvaccinated SARS-CoV-2-infected individuals without pneumonia. Among the 928 patients tested for autoantibodies against type I IFN, a quarter (234) were positive and were excluded.ResultsNo gene reached genome-wide significance. Under a recessive model, the most significant gene with at-risk variants was TLR7, with an OR of 27.68 (95%CI 1.5-528.7, P=1.1x10(-4)) for biochemically loss-of-function (bLOF) variants. We replicated the enrichment in rare predicted LOF (pLOF) variants at 13 influenza susceptibility loci involved in TLR3-dependent type I IFN immunity (OR=3.70[95%CI 1.3-8.2], P=2.1x10(-4)). This enrichment was further strengthened by (1) adding the recently reported TYK2 and TLR7 COVID-19 loci, particularly under a recessive model (OR=19.65[95%CI 2.1-2635.4], P=3.4x10(-3)), and (2) considering as pLOF branchpoint variants with potentially strong impacts on splicing among the 15 loci (OR=4.40[9%CI 2.3-8.4], P=7.7x10(-8)). Finally, the patients with pLOF/bLOF variants at these 15 loci were significantly younger (mean age [SD]=43.3 [20.3] years) than the other patients (56.0 [17.3] years; P=1.68x10(-5)).ConclusionsRare variants of TLR3- and TLR7-dependent type I IFN immunity genes can underlie life-threatening COVID-19, particularly with recessive inheritance, in patients under 60 years old