Vascular endothelial growth factor (VEGF ) receptor expression correlates with histologic grade and stage of colorectal cancer

Background: Colorectal carcinoma (CRC) is the seventh-most common malignancy and is the main cause of death in Iraq. The incidence of this cancer has increased sharply after the invasion of Iraq in 2003. Aim: To estimate immunohistochemical expression of vascular endothelial growth factor (VEGF) in CRC in relation to other parameters, such as grade and stage of tumour. Methods: Formalin fixed, paraffin-embedded blocks from 52 patients (27 male and 25 female) with CRC were included in this study. A group of 22 patients with non-cancerous colonic tissues were included as a control group. Avidin-biotin complex method was employed for immunohistochemical detection of VEGF. Results: VEGF immuno-expression was positive in 51.9% of CRC, while it was 18.2% in the normal colonic tissue (p<0.05). VEGF immunostaining was positively correlated with grade of colonic malignancy (p<0.05). Conclusion: These findings provide further evidence for the role of VEGF in the carcinogenesis of CRC. However, VEGF could not be well correlated with stage of tumour and hence may be a poor prognostic parameter of state of malignancy of colonic carcinoma.Keywords: colorectal carcinoma; VEGF; immunohistochemistr

An overview of the utilisation of microalgae biomass derived from nutrient recycling of wet market wastewater and slaughterhouse wastewater

Microalgae have high nutritional values for aquatic organisms compared to fish meal, because microalgae cells are rich in proteins, lipids, and carbohydrates. However, the high cost for the commercial production of microalgae biomass using fresh water or artificial media limits its use as fish feed. Few studies have investigated the potential of wet market wastewater and slaughterhouse wastewater for the production of microalgae biomass. Hence, this study aims to highlight the potential of these types of wastewater as an alternative superior medium for microalgae biomass as they contain high levels of nutrients required for microalgae growth. This paper focuses on the benefits of microalgae biomass produced during the phycore-mediation of wet market wastewater and slaughterhouse wastewater as fish feed. The extraction techniques for lipids and proteins as well as the studies conducted on the use of microalgae biomass as fish feed were reviewed. The results showed that microalgae biomass can be used as fish feed due to feed utilisation efficiency, physiological activity, increased resistance for several diseases, improved stress response, and improved protein retention

Up-regulated expression of LAMP2 and autophagy activity during neuroendocrine differentiation of prostate cancer LNCaP cells

Neuroendocrine (NE) prostate cancer (PCa) is a highly aggressive subtype of prostate cancer associated with resistance to androgen ablation therapy. In this study, we used LNCaP prostate cancer cells cultured in a serum-free medium for 6 days as a NE model of prostate cancer. Serum deprivation increased the expression of NE markers such as neuron-specific enolase (NSE) and βIII tubulin (βIII tub) and decreased the expression of the androgen receptor protein in LNCaP cells. Using cDNA microarrays, we compared gene expression profiles of NE cells and non-differentiated LNCaP cells. We identified up-regulation of 155 genes, among them LAMP2, a lysosomal membrane protein involved in lysosomal stability and autophagy. We then confirmed up-regulation of LAMP2 in NE cells by qRT-PCR, Western blot and confocal microscopy assays, showing that mRNA up-regulation correlated with increased levels of LAMP2 protein. Subsequently, we determined autophagy activity in NE cells by assessing the protein levels of SQSTM/p62 and LC3 by Western blot and LC3 and Atg5 mRNAs content by qRT-PCR. The decreased levels of SQSTM/p62 was accompanied by an enhanced expression of LC3 and ATG5, suggesting activation of autophagy in NE cells. Blockage of autophagy with 1μM AKT inhibitor IV, or by silencing Beclin 1 and Atg5, prevented NE cell differentiation, as revealed by decreased levels of the NE markers. In addition, AKT inhibitor IV as well as Beclin1 and Atg5 kwockdown attenuated LAMP2 expression in NE cells. On the other hand, LAMP2 knockdown by siRNA led to a marked blockage of autophagy, prevention of NE differentiation and decrease of cell survival. Taken together, these results suggest that LAMP2 overexpression assists NE differentiation of LNCaP cells induced by serum deprivation and facilitates autophagy activity in order to attain the NE phenotype and cell survival. LAMP2 could thus be a potential biomarker and potential target for NE prostate cancer

Properties of a Low-Carbon Binder-Based Mortar Made with Waste LCD Glass and Waste Rope (Nylon) Fibers

Carbon dioxide emissions are one of the problems that arouses the interest of scientists because of their harmful effects on the environment and climate. The construction sector, particularly the cement industry, is a significant source of CO2. On the other hand, solid waste constitutes a major problem facing governments due to the difficulty of decomposing it and the fact that it requires large areas for landfill. Among these wastes are LCD waste glass (WG) and used rope waste. Therefore, reusing these wastes, for example, in concrete technology, is a promising solution to reduce their environmental impact. Limited studies have dealt with the simultaneous utilization of glass waste as a substitute for cement and rope waste (nylon) fiber (WRF). Therefore, this study aimed to partially replace cement with WG with the addition of rope waste as fibers. Thirteen mixtures were poured: a reference mixture (without replacement or addition) and three other groups containing WG and WRF in proportions of 5, 15 and 25% by cement weight and 0.25, 0.5 and 0.75% by mortar weight, respectively. Flow rate, compression strength, flexural strength, dry density, water absorption, dynamic modulus of elasticity, ultrasonic pulse velocity and electrical resistivity were tested. The results indicate that the best ratio for replacing cement with WG without fibers was 5% of the weight of cement. However, using WRF increased the amount of glass replacement to 25%, with an improvement in strength and durability characteristics

Polycystic ovary syndrome

The document attached has been archived with permission from the editor of the Medical Journal of Australia. An external link to the publisher’s copy is included.Polycystic ovary syndrome (PCOS) affects 5-20% of women of reproductive age worldwide. The condition is characterized by hyperandrogenism, ovulatory dysfunction and polycystic ovarian morphology (PCOM) - with excessive androgen production by the ovaries being a key feature of PCOS. Metabolic dysfunction characterized by insulin resistance and compensatory hyperinsulinaemia is evident in the vast majority of affected individuals. PCOS increases the risk for type 2 diabetes mellitus, gestational diabetes and other pregnancy-related complications, venous thromboembolism, cerebrovascular and cardiovascular events and endometrial cancer. PCOS is a diagnosis of exclusion, based primarily on the presence of hyperandrogenism, ovulatory dysfunction and PCOM. Treatment should be tailored to the complaints and needs of the patient and involves targeting metabolic abnormalities through lifestyle changes, medication and potentially surgery for the prevention and management of excess weight, androgen suppression and/or blockade, endometrial protection, reproductive therapy and the detection and treatment of psychological features. This Primer summarizes the current state of knowledge regarding the epidemiology, mechanisms and pathophysiology, diagnosis, screening and prevention, management and future investigational directions of the disorder.Robert J Norman, Ruijin Wu and Marcin T Stankiewic

Mechanical properties, corrosion resistance and microstructural analysis of recycled aggregate concrete made with ceramic wall waste and ultrafine ceria

This study examines how incorporating ultrafine cerium dioxide particles (UFCe) into recycled coarse aggregate concrete affects its physical, mechanical, and long-term properties. No analogous research exists about the impact of ultrafine cerium dioxide particles on various characteristics of concrete containing recycled aggregates (RCA). UFCe was employed, and its mean particle size was 350 nm in different doses (0.0, 0.5, 1.0, and 1.5 % by cement weight) to explore its effect on the properties of concrete containing 25 % coarse aggregates (RCA) prepared from ceramic wall waste. The setting time, slump flow, porosity, water absorption, compressive and tensile strengths, electrical resistance, chloride penetration resistance, corrosion resistance, and microstructure analysis were investigated. The findings indicated that UFCe significantly enhanced the compressive and tensile strength while decreasing water absorption and pore ratio comparison to the control mixture after 90 days of curing. Moreover, all mixtures displayed significantly lower chloride penetration depth and corrosion rate than the reference mixture. The inclusion of UFCe additionally improved the microstructure due to the enhancement of the ultrafine particle hydration process. On the other hand, the optimum improvement of mechanical strength, durability properties, and microstructure was recorded at a UFCe replacement rate of 0.5 %. For example, the compressive and tensile strengths increased by 33 % and 9 %, respectively, while the total water absorption and migration coefficient were reduced by 42 % and 67 % at 90 days, respectively, compared with the reference sample

Does true Gleason pattern 3 merit its cancer descriptor?

Nearly five decades following its conception, the Gleason grading system remains a cornerstone in the prognostication and management of patients with prostate cancer. In the past few years, a debate has been growing whether Gleason score 3 + 3 = 6 prostate cancer is a clinically significant disease. Clinical, molecular and genetic research is addressing the question whether well characterized Gleason score 3 + 3 = 6 disease has the ability to affect the morbidity and quality of life of an individual in whom it is diagnosed. The consequences of treatment of Gleason score 3 + 3 = 6 disease are considerable; few men get through their treatments without sustaining some harm. Further modification of the classification of prostate cancer and dropping the label cancer for Gleason score 3 + 3 = 6 disease might be warranted

Surgical site infection after gastrointestinal surgery in high-income, middle-income, and low-income countries: a prospective, international, multicentre cohort study

Background: Surgical site infection (SSI) is one of the most common infections associated with health care, but its importance as a global health priority is not fully understood. We quantified the burden of SSI after gastrointestinal surgery in countries in all parts of the world. Methods: This international, prospective, multicentre cohort study included consecutive patients undergoing elective or emergency gastrointestinal resection within 2-week time periods at any health-care facility in any country. Countries with participating centres were stratified into high-income, middle-income, and low-income groups according to the UN's Human Development Index (HDI). Data variables from the GlobalSurg 1 study and other studies that have been found to affect the likelihood of SSI were entered into risk adjustment models. The primary outcome measure was the 30-day SSI incidence (defined by US Centers for Disease Control and Prevention criteria for superficial and deep incisional SSI). Relationships with explanatory variables were examined using Bayesian multilevel logistic regression models. This trial is registered with ClinicalTrials.gov, number NCT02662231. Findings: Between Jan 4, 2016, and July 31, 2016, 13 265 records were submitted for analysis. 12 539 patients from 343 hospitals in 66 countries were included. 7339 (58·5%) patient were from high-HDI countries (193 hospitals in 30 countries), 3918 (31·2%) patients were from middle-HDI countries (82 hospitals in 18 countries), and 1282 (10·2%) patients were from low-HDI countries (68 hospitals in 18 countries). In total, 1538 (12·3%) patients had SSI within 30 days of surgery. The incidence of SSI varied between countries with high (691 [9·4%] of 7339 patients), middle (549 [14·0%] of 3918 patients), and low (298 [23·2%] of 1282) HDI (p < 0·001). The highest SSI incidence in each HDI group was after dirty surgery (102 [17·8%] of 574 patients in high-HDI countries; 74 [31·4%] of 236 patients in middle-HDI countries; 72 [39·8%] of 181 patients in low-HDI countries). Following risk factor adjustment, patients in low-HDI countries were at greatest risk of SSI (adjusted odds ratio 1·60, 95% credible interval 1·05–2·37; p=0·030). 132 (21·6%) of 610 patients with an SSI and a microbiology culture result had an infection that was resistant to the prophylactic antibiotic used. Resistant infections were detected in 49 (16·6%) of 295 patients in high-HDI countries, in 37 (19·8%) of 187 patients in middle-HDI countries, and in 46 (35·9%) of 128 patients in low-HDI countries (p < 0·001). Interpretation: Countries with a low HDI carry a disproportionately greater burden of SSI than countries with a middle or high HDI and might have higher rates of antibiotic resistance. In view of WHO recommendations on SSI prevention that highlight the absence of high-quality interventional research, urgent, pragmatic, randomised trials based in LMICs are needed to assess measures aiming to reduce this preventable complication

Potential Use of Rendering Mortar Waste Powder as a Cement Replacement Material: Fresh, Mechanical, Durability and Microstructural Properties

The difficulty of decomposing solid waste over time has made it a significant global problem because of its environmental impact and the need for large areas for disposal. Among these residues is the waste of the rendering mortar that is produced (falls to the ground) while applied to wall surfaces. The quantity of these materials may reach 200 to 500 g/m2. As a result of local urban development (in Iraq), thousands of tons of these wastes are produced annually. On the other hand, the emission of greenhouse gases in the cement industry has had a great environmental impact. One of the solutions to this problem is to reduce the cement content in the mix by replacing it with less emissive materials. Residues from other industries are considered a relatively ideal option due to their disposal on the one hand and the reduction of harmful emissions of the cement industry on the other hand. Therefore, this research aims to reuse rendering mortar waste powder (RMWP) as a possible alternative to cement in mortar. RMWP replaced the cement in proportions (0, 10, 15, 20, 25, and 30% by weight). The flow rate, flexural and compressive strengths, ultrasonic pulse velocity, bulk density, dynamic modulus of elasticity, electrical resistivity, and water absorption tests of the produced mortar were executed. Microstructural analysis of the produced mortar was also investigated. Results indicated that, for sustainable development, an eco-friendly mortar can be made by replacing cement with RMWP at a rate of 15%, resulting in a 17% decrease in compressive strength while maintaining or improving durability properties. Moreover, the microstructure became denser and more homogeneous in the presence of RMWP

Prenatal exposures and exposomics of asthma

This review examines the causal investigation of preclinical development of childhood asthma using exposomic tools. We examine the current state of knowledge regarding early-life exposure to non-biogenic indoor air pollution and the developmental modulation of the immune system. We examine how metabolomics technologies could aid not only in the biomarker identification of a particular asthma phenotype, but also the mechanisms underlying the immunopathologic process. Within such a framework, we propose alternate components of exposomic investigation of asthma in which, the exposome represents a reiterative investigative process of targeted biomarker identification, validation through computational systems biology and physical sampling of environmental medi