Microfluidics for Advanced Drug Delivery Systems.

Considerable efforts have been devoted towards developing effective drug delivery methods. Microfluidic systems, with their capability for precise handling and transport of small liquid quantities, have emerged as a promising platform for designing advanced drug delivery systems. Thus, microfluidic systems have been increasingly used for fabrication of drug carriers or direct drug delivery to a targeted tissue. In this review, the recent advances in these areas are critically reviewed and the shortcomings and opportunities are discussed. In addition, we highlight the efforts towards developing smart drug delivery platforms with integrated sensing and drug delivery components

Warp and Weft Wiring method for rapid, modifiable, self-aligned, and bonding-free fabrication of multi electrodes microfluidic sensors

The need for rapid fabrication of microfluidic devices has become increasingly critical as microfluidics become part of biomedical sensors. Using Warp and Weft Wiring (WWW) of copper wires, this paper presents a novel low-cost method for rapid, self-aligned, bonding-free, and modifiable fabrication of multi-electrodes microfluidic sensors. All the proposed features are promising and highly recommended for the development of Point-of-Care Tests (POCTs), while most of the conventional methods have low chances of coming out of the research labs and play no role in POCTs development. To have an experimental proof of concept, the proposed chip was fabricated and then tested with two sets of experiments that showed the potential applications of water quality management, hygiene, biomedical impedance measurement, cell analysis, flow cytometry, etc

Additively manufactured porous scaffolds by design for treatment of bone defects

There has been increasing attention to produce porous scaffolds that mimic human bone properties for enhancement of tissue ingrowth, regeneration, and integration. Additive manufacturing (AM) technologies, i.e., three dimensional (3D) printing, have played a substantial role in engineering porous scaffolds for clinical applications owing to their high level of design and fabrication flexibility. To this end, this review article attempts to provide a detailed overview on the main design considerations of porous scaffolds such as permeability, adhesion, vascularisation, and interfacial features and their interplay to affect bone regeneration and osseointegration. Physiology of bone regeneration was initially explained that was followed by analysing the impacts of porosity, pore size, permeability and surface chemistry of porous scaffolds on bone regeneration in defects. Importantly, major 3D printing methods employed for fabrication of porous bone substitutes were also discussed. Advancements of MA technologies have allowed for the production of bone scaffolds with complex geometries in polymers, composites and metals with well-tailored architectural, mechanical, and mass transport features. In this way, a particular attention was devoted to reviewing 3D printed scaffolds with triply periodic minimal surface (TPMS) geometries that mimic the hierarchical structure of human bones. In overall, this review enlighten a design pathway to produce patient-specific 3D-printed bone substitutions with high regeneration and osseointegration capacity for repairing large bone defects

Recent microfluidic innovations for sperm sorting

Sperm selection is a clinical need for guided fertilization in men with low-quality semen. In this regard, microfluidics can provide an enabling platform for the precise manipulation and separation of high-quality sperm cells through applying various stimuli, including chemical agents, mechanical forces, and thermal gradients. In addition, microfluidic platforms can help to guide sperms and oocytes for controlled in vitro fertilization or sperm sorting using both passive and active methods. Herein, we present a detailed review of the use of various microfluidic methods for sorting and categorizing sperms for different applications. The advantages and disadvantages of each method are further discussed and future perspectives in the field are given

A switchable pH-differential unitized regenerative fuel cell with high performance

Regenerative fuel cells are a potential candidate for future energy storage, but their applications are limited by the high cost and poor round-trip efficiency. Here we present a switchable pH-differential unitized regenerative fuel cell capable of addressing both the obstacles. Relying on a membraneless laminar flow-based design, pH environments in the cell are optimized independently for different electrode reactions and are switchable together with the cell process to ensure always favorable thermodynamics for each electrode reaction. Benefiting from the thermodynamic advantages of the switchable pH-differential arrangement, the cell allows water electrolysis at a voltage of 0.57 V, and a fuel cell open circuit voltage of 1.89 V, rendering round-trip efficiencies up to 74%. Under room conditions, operating the cell in fuel cell mode yields a power density of 1.3 W cm¯², which is the highest performance to date for laminar flow-based cells and is comparable to state-of-the-art polymer electrolyte membrane fuel cells

Human Organ Culture: Updating the Approach to Bridge the Gap from In Vitro to In Vivo in Inflammation, Cancer, and Stem Cell Biology

Human studies, critical for developing new diagnostics and therapeutics, are limited by ethical and logistical issues, and preclinical animal studies are often poor predictors of human responses. Standard human cell cultures can address some of these concerns but the absence of the normal tissue microenvironment can alter cellular responses. Three-dimensional cultures that position cells on synthetic matrices, or organoid or organ-on-a-chip cultures, in which different cell spontaneously organize contacts with other cells and natural matrix only partly overcome this limitation. Here, we review how human organ cultures (HOCs) can more faithfully preserve in vivo tissue architecture and can better represent disease-associated changes. We will specifically describe how HOCs can be combined with both traditional and more modern morphological techniques to reveal how anatomic location can alter cellular responses at a molecular level and permit comparisons among different cells and different cell types within the same tissue. Examples are provided involving use of HOCs to study inflammation, cancer, and stem cell biology.The authors would like to express their gratitude to The National Institute for Health Research (NIHR) Cambridge Biomedical Research Centre (RA-L, JB)

The effects of electrode and catalyst selection on microfluidic fuel cell performance

A fuel cell can be best defined as an electrochemical converter of fuel and oxidant of chemical energy to electrical energy. The important components of micro fuel cells are the electrodes and catalysts because the kinetics and rates of the electrochemical reactions depend on their materials. All fuel cells consist of two electrodes: the anode, where fuel oxidation takes place, and the cathode, which is used to reduce the oxidants. The present review article highlights the use of a range of electrodes made up of different materials, a variety of catalysts that have been used in previous studies, and their fabrication materials and approaches. In this article, electrodes and catalysts are classified into two types based on the design approach applied to produce the micro fuel cell: micro fuel cell design and conventional assembly design. Most previous studies on fuel cells have demonstrated that the construction and position of the electrodes play crucial roles in improving the performance of micro fuel cells

Fiber Optic Sensor Designs and Luminescence-based Methods for the detection of Oxygen and pH measurement

Optical, and especially fiber optic techniques for chemical sensing have become very attractive in recent decades for a wide variety of biomedical and industrial processes and considerable progress in research in this field has been seen, evidenced by the significant number of papers published over several decades, commercial products developed and marketed and which continuing to be produced. This work extends the body of knowledge in the field and focuses on two industrially-important ‘chemical’ measurands: the determination of pH level and oxygen concentration (O2) - both are critically important for a broad range of applications globally, in fields as diverse at the life sciences, environmental monitoring, biomedical research and thus widely across industry. The many different optical platform designs and fabrication methods that have been developed are considered, including those for commercial applications, recognizing the wide range of industrial and scientific uses, and their performance compared. Further, the effect of specific fiber structures on sensor performance, e.g. on sensitivity, response time and long-term stability, and possible applications also has been analyzed. Applications are seen in difficult and ‘niche’ measurement environments to which conventional sensors are often not well suited, taking advantage of their lightweight nature, ease of miniaturization, potential to be multiplexed and low cost. Through a discussion of representative techniques that have reached commercial development, a comprehensive and state-of-the-art view of this exciting and important field is possible

Microfluidic fuel cell for off-the-grid applications

The present doctoral thesis studies air-breathing microfluidic fuel cells with separated fuel and electrolyte streams as well as a membraneless fuel cell with selective electrodes. In order to gain more insight into the physio-chemical reactions, numerical simulation of the in-house developed air-breathing microfluidic fuel cell is formulated and solved using COMSOL Multiphysics. The results from the simulation show that fuel stream at the anode side and its interaction with the electrolyte stream has significant impact on the total fuel cell performance. As the first step for improving the hydrodynamic manipulation of the fuel stream, a flow-through porous anode is introduced. The effects of flow architecture on fuel utilization and the whole cell performance are investigated. Experimental results show that the flow-through porous anode improves the cell current in a long-term performance test as compared to the conventional design with flow-over planar anode. Because of the improved current generation, the rate of carbon dioxide generation in the cell increases. At high current densities, carbon dioxide produced in the channel emerges as bubbles that block and hinders reactant transport to the active sites of the anode.DOCTOR OF PHILOSOPHY (MAE