34 research outputs found
Nanostructured luminescently labeled nucleic acids
Important and emerging trends at the interface of luminescence, nucleic acids and nanotechnology
are: (i) the conventional luminescence labeling of nucleic acid nanostructures (e.g. DNA tetrahedron);
(ii) the labeling of bulk nucleic acids (e.g. singleâstranded DNA, doubleâstranded DNA) with
nanostructured luminescent labels (e.g. copper nanoclusters); and (iii) the labeling of nucleic acid
nanostructures (e.g. origami DNA) with nanostructured luminescent labels (e.g. silver
nanoclusters). This review surveys recent advances in these three different approaches to the
generation of nanostructured luminescently labeled nucleic acids, and includes both direct and
indirect labeling methods
scFv-based fluorogen activating proteins and variable domain inhibitors as fluorescent biosensor platforms
High-throughput flow cytometry compatible biosensor based on fluorogen activating protein technology
Fluorogen activating proteins in flow cytometry for the study of surface molecules and receptors
Fluorogen-Activating scFv Biosensors Target Surface Markers on Live Cells Via Streptavidin or Single-Chain Avidin
A Convenient Preparation of Multi-Spectral Microparticles by Bacteria-Mediated Assemblies of Conjugated Polymer Nanoparticles for Cell Imaging and Barcoding
Nanoscale DNA tetrahedra improve biomolecular recognition on patterned surfaces.
The bottomâup approach of DNA nanoâbiotechnology can create biomaterials with defined properties relevant for a wide range of applications. This report describes nanoscale DNA tetrahedra that are beneficial to the field of biosensing and the targeted immobilization of biochemical receptors on substrate surfaces. The DNA nanostructures act as immobilization agents that are able to present individual molecules at a defined nanoscale distance to the solvent thereby improving biomolecular recognition of analytes. The tetrahedral display devices are selfâassembled from four oligonucleotides. Three of the four tetrahedron vertices are equipped with disulfide groups to enable oriented binding to gold surfaces. The fourth vertex at the top of the bound tetrahedron presents the biomolecular receptor to the solvent. In assays testing the molecular accessibility via DNA hybridization and protein capturing, tetrahedronâtethered receptors outperformed conventional immobilization approaches with regard to specificity and amount of captured polypeptide by a factor of up to seven. The bottomâup strategy of creating DNA tetrahedrons is also compatible with the topâdown route of nanopatterning of inorganic substrates, as demonstrated by the specific coating of microâ to nanoscale gold squares amid surrounding blank or poly(ethylene glycol)âpassivated glass surfaces. DNA tetrahedra can create biofunctionalized surfaces of rationally designed properties that are of relevance in analytical chemistry, cell biology, and singleâmolecule biophysics