Разработка композиций готовых форм антисептиков на основе Циторецифена

Проблематика. Практичне використання біологічно активних субстанцій ускладнюється їх нестабільністю, складністю попередньої підготовки та нанесення. Використання допоміжних речовин дає можливість створити зручну для застосування готову форму препарату та скорегувати активність субстанції. Мета дослідження. Широкий спектр антимікробної дії ферментного препарату Циторецифен дає підстави розглядати його як перспективну основу м’яких готових форм препарату. Тому мета роботи полягає в розробленні композиції м’якої готової форми препарату-антисептика для медицини та ветеринарії на основі Циторецифену. Методика реалізації. Літичну активність ферментного комплексу та його готових форм визначали турбідіметричним методом за здатністю до деградації суспензії мікробної тест-культури. Результати дослідження. Визначено вплив основних формоутворюючих допоміжних речовин (проксанолу 268, ПЕО-400, ПЕГ-600, ПЕГ-1000 і гліцерину) консервантів і анестетиків (мірамістину, офлоксацину, димексиду, лідокаїну) на літичну активність бактеріолітичного ферментного препарату Циторецифен. Встановлено відсутність істотного негативного впливу вибраних допоміжних речовин на активність ферменту, а отже, можливість їх використання у складі готових форм препарату. Висновки. Запропоновано композицію антисептичної мазі для ветеринарного та медичного застосування на основі поліоксиетиленів, проксанолу 268, гліцерину, лідокаїну та офлоксацину, що містить 40 мг/мл ферментного препарату Циторецифен.Background. The practical use of bioactive substances becomes complicated by their instability, complication of pre-treatment and causing. The use of auxiliary matters is given by possibility to create the comfortable for application prepared form of preparation and correct activity of substance. Objective. The wide spectrum of antimicrobial action of enzyme preparation of Cytorecifen provides grounds to examine him as perspective basis for the soft prepared forms of preparation. Therefore, the purpose of work is to develop composition of the soft prepared form of preparation-antiseptic for medicine and veterinary on the basis of Cytorecifen. Methods. The lytic activity of enzyme complex and it the prepared forms determined a turbidimetric method by the capacity of degradation of suspension of microbe test-culture. Results. It was determined the influence of basic shape-generating auxiliary materials (proksanol-268, PEO-400, PEG-600, PEG-1000, and glycerin), preservatives and anesthetic (miramistin, ofloksacyn, dimeksid, lidocaine) on lytic activity of bacteriolytic enzyme preparation of Cytorecifen. It was proved an absence of substantial negative influence of the chosen auxiliary materials on activity of enzyme, and consequently possibility of their use in composition the prepared forms of preparation. Conclusions. The composition of antiseptic ointment is offered for veterinary and medical application on the basis of polyoxyethylen, proksanola 268, glycerin, lidocaine and ofloksacyn, containing 40 mg/ml of enzyme preparation of Cytorecifen.Проблематика. Практическое использование биологически активных субстанций усложняется их нестабильностью, сложностью предварительной подготовки и нанесения. Использование вспомогательных веществ дает возможность создать удобную для применения готовую форму препарата и скорректировать активность субстанции. Цель исследования. Широкий спектр антимикробного действия ферментного препарата Циторецифен дает основания рассматривать его как перспективную основу мягких готовых форм препарата. Поэтому целью работы является разработка композиции мягкой готовой формы препарата-антисептика для медицины и ветеринарии на основе Циторецифена. Методика реализации. Литическую активность ферментного комплекса и его готовых форм определяли турбидиметрическим методом по способности к деградации суспензии микробной тест-культуры. Результаты исследований. Определенно влияние основных формообразующих вспомогательных веществ (проксанола 268, ПЕО-400, ПЭГ-600, ПЭГ-1000 и глицерина) консервантов и анестетиков (мирамистина, офлоксацина, димексида, лидокаина) на литическую активность бактериолитического ферментного препарата Циторецифен. Установлено отсутствие существенного негативного влияния выбранных вспомогательных веществ на активность фермента, а следовательно, возможность их использования в составе готовых форм препарата. Выводы. Предложена композиция антисептической мази для ветеринарного и медицинского применения на основе полиоксиэтиленов, проксанола 268, глицерина, лидокаина и офлоксацина, содержащая 40 мг/мл ферментного препарата Циторецифен

Indicators of the cytokine system in practically healthy women of different ages and interrelation with the emotional state

Numerous studies show the role of the cytokine network in the pathogenesis of anxiety and depression. However, at present, studies of the correlation between the levels of pro-inflammatory and antiinflammatory cytokines and the level of emotional stress are rather few. The aim of the study was to analyze the serum levels of pro-inflammatory and anti-inflammatory cytokines and the emotional state in apparently healthy women depending on age. Serum levels were tested IL-1β, IL-6, IL-17, IFNγ, IL-10 and IL-4 in 100 apparently healthy women, who were divided into 3 groups depending on age (WHO): 18-44 (young age) 30 people, 45-59 (middle age) 40 people, 60-74 (old age) 30 people (sandwich variant of enzyme-linked immunosorbent assay, pg/mL). To assess the emotional component of health, all the subjects passed the questionnaire SF-36 “Assessment of the quality of life”. Statistical processing of the obtained data was carried out using the analytical software IBM SPSS Statistics, 22.0. In practically healthy women, an increase in the values of IL-1β and IL-6 was found in the elderly group (p < 0.05), while no differences were found between the groups of young and middle age. The level of IFNγ in all age groups of women did not differ significantly. At the same time, in the elderly group, the levels of IFNγ in 40% ranged from 1.04 to 8.76 pg/mL, and in 60% of women – from 24.85 to 28.5 pg/mL. IL-17 was also high (p < 0.05-0.01) in the group of women aged 60-74. In the anti-inflammatory link, the opposite picture was observed, for example, in young and middle-aged women, the levels of IL-10 and IL-4 were higher than in the elderly group. Thus, the analysis made it possible to state that the parameters of the cytokine profile and emotional state in women are associated with age

Transverse-momentum-dependent Multiplicities of Charged Hadrons in Muon-Deuteron Deep Inelastic Scattering

A semi-inclusive measurement of charged hadron multiplicities in deep inelastic muon scattering off an isoscalar target was performed using data collected by the COMPASS Collaboration at CERN. The following kinematic domain is covered by the data: photon virtuality $Q^{2}>1$ (GeV/$c$)$^2$, invariant mass of the hadronic system $W > 5$ GeV/$c^2$, Bjorken scaling variable in the range $0.003 < x < 0.4$, fraction of the virtual photon energy carried by the hadron in the range $0.2 < z < 0.8$, square of the hadron transverse momentum with respect to the virtual photon direction in the range 0.02 (GeV/$c)^2 < P_{\rm{hT}}^{2} < 3$ (GeV/$c$)$^2$. The multiplicities are presented as a function of $P_{\rm{hT}}^{2}$ in three-dimensional bins of $x$, $Q^2$, $z$ and compared to previous semi-inclusive measurements. We explore the small-$P_{\rm{hT}}^{2}$ region, i.e. $P_{\rm{hT}}^{2} < 1$ (GeV/$c$)$^2$, where hadron transverse momenta are expected to arise from non-perturbative effects, and also the domain of larger $P_{\rm{hT}}^{2}$, where contributions from higher-order perturbative QCD are expected to dominate. The multiplicities are fitted using a single-exponential function at small $P_{\rm{hT}}^{2}$ to study the dependence of the average transverse momentum $\langle P_{\rm{hT}}^{2}\rangle$ on $x$, $Q^2$ and $z$. The power-law behaviour of the multiplicities at large $P_{\rm{hT}}^{2}$ is investigated using various functional forms. The fits describe the data reasonably well over the full measured range.Comment: 28 pages, 20 figure

Light isovector resonances in π-p →π-π-π+p at 190 GeV/c

We have performed the most comprehensive resonance-model fit of π-π-π+ states using the results of our previously published partial-wave analysis (PWA) of a large data set of diffractive-dissociation events from the reaction π-+p→π-π-π++precoil with a 190 GeV/c pion beam. The PWA results, which were obtained in 100 bins of three-pion mass, 0.5<2.5 GeV/c2, and simultaneously in 11 bins of the reduced four-momentum transfer squared, 0.1<1.0 (GeV/c)2, are subjected to a resonance-model fit using Breit-Wigner amplitudes to simultaneously describe a subset of 14 selected waves using 11 isovector light-meson states with JPC=0-+, 1++, 2++, 2-+, 4++, and spin-exotic 1-+ quantum numbers. The model contains the well-known resonances π(1800), a1(1260), a2(1320), π2(1670), π2(1880), and a4(2040). In addition, it includes the disputed π1(1600), the excited states a1(1640), a2(1700), and π2(2005), as well as the resonancelike a1(1420). We measure the resonance parameters mass and width of these objects by combining the information from the PWA results obtained in the 11 t′ bins. We extract the relative branching fractions of the ρ(770)π and f2(1270)π decays of a2(1320) and a4(2040), where the former one is measured for the first time. In a novel approach, we extract the t′ dependence of the intensity of the resonances and of their phases. The t′ dependence of the intensities of most resonances differs distinctly from the t′ dependence of the nonresonant components. For the first time, we determine the t′ dependence of the phases of the production amplitudes and confirm that the production mechanism of the Pomeron exchange is common to all resonances. We have performed extensive systematic studies on the model dependence and correlations of the measured physical parameters

Double J/ψJ/\psi production in pion-nucleon scattering at COMPASS

We present the study of the production of double $J/\psi$ mesons using COMPASS data collected with a 190 GeV/$c$ $\pi^-$ beam scattering off NH$_{3}$, Al and W targets. Kinematic distributions of the collected double $J/\psi$ events are analysed, and the double $J/\psi$ production cross section is estimated for each of the COMPASS targets. The results are compared to predictions from single- and double-parton scattering models as well as the pion intrinsic charm and the tetraquark exotic resonance hypotheses. It is demonstrated that the single parton scattering production mechanism gives the dominant contribution that is sufficient to describe the data. An upper limit on the double intrinsic charm content of pion is evaluated. No significant signatures that could be associated with exotic tetraquarks are found in the double $J/\psi$ mass spectrum.Comment: 12 pages, 4 figure

Feasibility studies for the measurement of time-like proton electromagnetic form factors from p¯ p→ μ+μ- at P ¯ ANDA at FAIR

This paper reports on Monte Carlo simulation results for future measurements of the moduli of time-like proton electromagnetic form factors, | GE| and | GM| , using the p¯ p→ μ+μ- reaction at P ¯ ANDA (FAIR). The electromagnetic form factors are fundamental quantities parameterizing the electric and magnetic structure of hadrons. This work estimates the statistical and total accuracy with which the form factors can be measured at P ¯ ANDA , using an analysis of simulated data within the PandaRoot software framework. The most crucial background channel is p¯ p→ π+π-, due to the very similar behavior of muons and pions in the detector. The suppression factors are evaluated for this and all other relevant background channels at different values of antiproton beam momentum. The signal/background separation is based on a multivariate analysis, using the Boosted Decision Trees method. An expected background subtraction is included in this study, based on realistic angular distributions of the background contribution. Systematic uncertainties are considered and the relative total uncertainties of the form factor measurements are presented

Longitudinal double-spin asymmetry A1pand spin-dependent structure function g1pof the proton at small values of x and Q2

We present a precise measurement of the proton longitudinal double-spin asymmetry Ap1 and the proton spin-dependent structure function gp 1 at photon virtualities 0.006 (GeV/c)2 < Q 2 < 1 (GeV/c)2 in the Bjorken x range of 4 7 10 125 < x < 4 7 10 122. The results are based on data collected by the COMPASS Collaboration at CERN using muon beam energies of 160 GeV and 200 GeV. The statistical precision is more than tenfold better than that of the previous measurement in this region. In the whole range of x, the measured values of Ap1 and gp1 are found to be positive. It is for the first time that spin effects are found at such low values of

Precision resonance energy scans with the PANDA experiment at FAIR: Sensitivity study for width and line shape measurements of the X(3872)

This paper summarises a comprehensive Monte Carlo simulation study for precision resonance energy scan measurements. Apart from the proof of principle for natural width and line shape measurements of very narrow resonances with PANDA, the achievable sensitivities are quantified for the concrete example of the charmonium-like X(3872) state discussed to be exotic, and for a larger parameter space of various assumed signal cross-sections, input widths and luminosity combinations. PANDA is the only experiment that will be able to perform precision resonance energy scans of such narrow states with quantum numbers of spin and parities that differ from J P C = 1 - -