Perímetro blando : Un recorrido por los bordes

Se presentan aquí los Trabajos de Graduación correspondientes a la Licenciatura en Artes Plásticas con Orientación en Grabado y Arte Impreso y con Orientación en Pintura de la Facultad de Artes de la UNLP. Esta propuesta colectiva es parte de un proyecto institucional que desde el año 2020 desarrolla dichos trabajos de finalización de la carrera en el marco de los Talleres de Producción Plástica, con el acompañamiento de los equipos docentes.Texto curatorial de la exhibición de los trabajos de graduación correspondientes a la Licenciatura en Artes Plásticas, con Orientación en Grabado y Arte Impreso de la Facultad de Artes (UNLP).Facultad de Arte

Metodología para una investigación acción visual : "El estilo personal en la producción artística individual"

Diversas codificaciones, como los estilos históricos, se originan en la cristalización de ciertas combinaciones de opciones expresivas de los elementos del lenguaje visual. Más allá del marco cultural, entorno y época, motivo por el que comprendemos e interpretamos de las diversas plasmaciones como sistemas aceptados, entendemos al estilo como un “conjunto de elementos que opera mediante una lógica interna de forma sistemática u orgánica, que permite la futura lectura y posterior resignificación a lo largo de la historia”. En la actualidad, donde abunda el recurso de la cita, la hibridación y la transposición el estilo ha recobrado trascendencia como medio y fin en diversas manifestaciones artísticas abriendo el campo a nuevas y múltiples lecturas en diferentes niveles. El presente trabajo busca explicar un método de investigación aplicado centrado en la acción y la producción visual como factor principal de acercamiento al objeto de estudio: en nuestro caso el estilo personal en la producción artística individual. Las experiencias que aquí se mencionan son parte del proyecto de investigación UNA Nº34/0336 dirigido por la Dra. M. Bibiana Anguio.Eje 1: Educación e investigación. Estrategias para la producción de tramas de sentido en las artes y los diseños.Facultad de Bellas Arte

Metodología para una investigación acción visual : "El estilo personal en la producción artística individual"

Diversas codificaciones, como los estilos históricos, se originan en la cristalización de ciertas combinaciones de opciones expresivas de los elementos del lenguaje visual. Más allá del marco cultural, entorno y época, motivo por el que comprendemos e interpretamos de las diversas plasmaciones como sistemas aceptados, entendemos al estilo como un “conjunto de elementos que opera mediante una lógica interna de forma sistemática u orgánica, que permite la futura lectura y posterior resignificación a lo largo de la historia”. En la actualidad, donde abunda el recurso de la cita, la hibridación y la transposición el estilo ha recobrado trascendencia como medio y fin en diversas manifestaciones artísticas abriendo el campo a nuevas y múltiples lecturas en diferentes niveles. El presente trabajo busca explicar un método de investigación aplicado centrado en la acción y la producción visual como factor principal de acercamiento al objeto de estudio: en nuestro caso el estilo personal en la producción artística individual. Las experiencias que aquí se mencionan son parte del proyecto de investigación UNA Nº34/0336 dirigido por la Dra. M. Bibiana Anguio.Eje 1: Educación e investigación. Estrategias para la producción de tramas de sentido en las artes y los diseños.Facultad de Bellas Arte

Inmanencias: proyecto para una galería a cielo abierto

En el presente trabajo queremos compartir una experiencia colectiva e innovadora en la que participamos docentes y estudiantes de la Cátedra de Pintura Complementaria de la FBA y del Área de Artes Visuales del Liceo Víctor Mercante; la misma surge de la invitación del cuerpo docente de la Cátedra de Pintura Complementaria de la FBA para participar en su proyecto denominado “Inmanencias” en el marco de la Cuarta Bienal de Arte y Cultura de la UNLP (4 BUAyC). La propuesta que definía claramente sus objetivos desde las primeras líneas del proyecto, consistía en el armado y montaje de una galería a cielo abierto en el muro exterior del Liceo; propuesta que permitió desarrollar una actividad entre dos instituciones, poner en diálogo los trabajos realizados por estudiantes de ambas unidades académicas, compartir una jornada de montaje más allá del espacio del aula y darle a las imágenes el carácter de públicas.Eje 5: Las prácticas de producción artística en ámbitos de talleres académicos y otros.Facultad de Bellas Arte

La radiación sale a la comunidad : Parte II

Este proyecto viene realizándose desde 2016, ya que es de suma relevancia esta temática llevada a la comunidad y así fortalecer el sistema de Salud Público. La radioprotección es un tema poco abordado en proyectos de extensión universitaria y salud pública. Los pacientes conocen lo que es una radiografía pero la mayoría desconoce que se utilizan radiaciones ionizantes, las cuales son acumulativas durante toda la vida del ser humano y que pueden causar posibles efectos perjudiciales sino se utilizan las medidas de radioprotección en forma correcta. Objetivos: Lograr la concientización y difusión actuando en la prevención de posibles efectos provocados por la acción de las radiaciones ionizantes en pacientes de la comunidad de Lisandro Olmos y Berisso.Facultad de Odontologí

Epigenetic Natural Variation in Arabidopsis thaliana

Cytosine methylation of repetitive sequences is widespread in plant genomes, occurring in both symmetric (CpG and CpNpG) as well as asymmetric sequence contexts. We used the methylation-dependent restriction enzyme McrBC to profile methylated DNA using tiling microarrays of Arabidopsis Chromosome 4 in two distinct ecotypes, Columbia and Landsberg erecta. We also used comparative genome hybridization to profile copy number polymorphisms. Repeated sequences and transposable elements (TEs), especially long terminal repeat retrotransposons, are densely methylated, but one third of genes also have low but detectable methylation in their transcribed regions. While TEs are almost always methylated, genic methylation is highly polymorphic, with half of all methylated genes being methylated in only one of the two ecotypes. A survey of loci in 96 Arabidopsis accessions revealed a similar degree of methylation polymorphism. Within-gene methylation is heritable, but is lost at a high frequency in segregating F2 families. Promoter methylation is rare, and gene expression is not generally affected by differences in DNA methylation. Small interfering RNA are preferentially associated with methylated TEs, but not with methylated genes, indicating that most genic methylation is not guided by small interfering RNA. This may account for the instability of gene methylation, if occasional failure of maintenance methylation cannot be restored by other means

Genome-wide characterization of simple sequence repeats in cucumber (Cucumis sativus L.)

<p>Abstract</p> <p>Background</p> <p>Cucumber, <it>Cucumis sativus </it>L. is an important vegetable crop worldwide. Until very recently, cucumber genetic and genomic resources, especially molecular markers, have been very limited, impeding progress of cucumber breeding efforts. Microsatellites are short tandemly repeated DNA sequences, which are frequently favored as genetic markers due to their high level of polymorphism and codominant inheritance. Data from previously characterized genomes has shown that these repeats vary in frequency, motif sequence, and genomic location across taxa. During the last year, the genomes of two cucumber genotypes were sequenced including the Chinese fresh market type inbred line '9930' and the North American pickling type inbred line 'Gy14'. These sequences provide a powerful tool for developing markers in a large scale. In this study, we surveyed and characterized the distribution and frequency of perfect microsatellites in 203 Mbp assembled Gy14 DNA sequences, representing 55% of its nuclear genome, and in cucumber EST sequences. Similar analyses were performed in genomic and EST data from seven other plant species, and the results were compared with those of cucumber.</p> <p>Results</p> <p>A total of 112,073 perfect repeats were detected in the Gy14 cucumber genome sequence, accounting for 0.9% of the assembled Gy14 genome, with an overall density of 551.9 SSRs/Mbp. While tetranucleotides were the most frequent microsatellites in genomic DNA sequence, dinucleotide repeats, which had more repeat units than any other SSR type, had the highest cumulative sequence length. Coding regions (ESTs) of the cucumber genome had fewer microsatellites compared to its genomic sequence, with trinucleotides predominating in EST sequences. AAG was the most frequent repeat in cucumber ESTs. Overall, AT-rich motifs prevailed in both genomic and EST data. Compared to the other species examined, cucumber genomic sequence had the highest density of SSRs (although comparable to the density of poplar, grapevine and rice), and was richest in AT dinucleotides. Using an electronic PCR strategy, we investigated the polymorphism between 9930 and Gy14 at 1,006 SSR loci, and found unexpectedly high degree of polymorphism (48.3%) between the two genotypes. The level of polymorphism seems to be positively associated with the number of repeat units in the microsatellite. The <it>in silico </it>PCR results were validated empirically in 660 of the 1,006 SSR loci. In addition, primer sequences for more than 83,000 newly-discovered cucumber microsatellites, and their exact positions in the Gy14 genome assembly were made publicly available.</p> <p>Conclusions</p> <p>The cucumber genome is rich in microsatellites; AT and AAG are the most abundant repeat motifs in genomic and EST sequences of cucumber, respectively. Considering all the species investigated, some commonalities were noted, especially within the monocot and dicot groups, although the distribution of motifs and the frequency of certain repeats were characteristic of the species examined. The large number of SSR markers developed from this study should be a significant contribution to the cucurbit research community.</p

Sorghum Genome Sequencing by Methylation Filtration

Sorghum bicolor is a close relative of maize and is a staple crop in Africa and much of the developing world because of its superior tolerance of arid growth conditions. We have generated sequence from the hypomethylated portion of the sorghum genome by applying methylation filtration (MF) technology. The evidence suggests that 96% of the genes have been sequence tagged, with an average coverage of 65% across their length. Remarkably, this level of gene discovery was accomplished after generating a raw coverage of less than 300 megabases of the 735-megabase genome. MF preferentially captures exons and introns, promoters, microRNAs, and simple sequence repeats, and minimizes interspersed repeats, thus providing a robust view of the functional parts of the genome. The sorghum MF sequence set is beneficial to research on sorghum and is also a powerful resource for comparative genomics among the grasses and across the entire plant kingdom. Thousands of hypothetical gene predictions in rice and Arabidopsis are supported by the sorghum dataset, and genomic similarities highlight evolutionarily conserved regions that will lead to a better understanding of rice and Arabidopsis

Sequencing of diverse mandarin, pummelo and orange genomes reveals complex history of admixture during citrus domestication

Cultivated citrus are selections from, or hybrids of, wild progenitor species whose identities and contributions to citrus domestication remain controversial. Here we sequence and compare citrus genomes-a high-quality reference haploid clementine genome and mandarin, pummelo, sweet-orange and sour-orange genomes-and show that cultivated types derive from two progenitor species. Although cultivated pummelos represent selections from one progenitor species, Citrus maxima, cultivated mandarins are introgressions of C. maxima into the ancestral mandarin species Citrus reticulata. The most widely cultivated citrus, sweet orange, is the offspring of previously admixed individuals, but sour orange is an F1 hybrid of pure C. maxima and C. reticulata parents, thus implying that wild mandarins were part of the early breeding germplasm. A Chinese wild 'mandarin' diverges substantially from C. reticulata, thus suggesting the possibility of other unrecognized wild citrus species. Understanding citrus phylogeny through genome analysis clarifies taxonomic relationships and facilitates sequence-directed genetic improvement

Microsatellite isolation and marker development in carrot - genomic distribution, linkage mapping, genetic diversity analysis and marker transferability across Apiaceae

<p>Abstract</p> <p>Background</p> <p>The Apiaceae family includes several vegetable and spice crop species among which carrot is the most economically important member, with ~21 million tons produced yearly worldwide. Despite its importance, molecular resources in this species are relatively underdeveloped. The availability of informative, polymorphic, and robust PCR-based markers, such as microsatellites (or SSRs), will facilitate genetics and breeding of carrot and other Apiaceae, including integration of linkage maps, tagging of phenotypic traits and assisting positional gene cloning. Thus, with the purpose of isolating carrot microsatellites, two different strategies were used; a hybridization-based library enrichment for SSRs, and bioinformatic mining of SSRs in BAC-end sequence and EST sequence databases. This work reports on the development of 300 carrot SSR markers and their characterization at various levels.</p> <p>Results</p> <p>Evaluation of microsatellites isolated from both DNA sources in subsets of 7 carrot F₂mapping populations revealed that SSRs from the hybridization-based method were longer, had more repeat units and were more polymorphic than SSRs isolated by sequence search. Overall, 196 SSRs (65.1%) were polymorphic in at least one mapping population, and the percentage of polymophic SSRs across F₂populations ranged from 17.8 to 24.7. Polymorphic markers in one family were evaluated in the entire F₂, allowing the genetic mapping of 55 SSRs (38 codominant) onto the carrot reference map. The SSR loci were distributed throughout all 9 carrot linkage groups (LGs), with 2 to 9 SSRs/LG. In addition, SSR evaluations in carrot-related taxa indicated that a significant fraction of the carrot SSRs transfer successfully across Apiaceae, with heterologous amplification success rate decreasing with the target-species evolutionary distance from carrot. SSR diversity evaluated in a collection of 65 <it>D. carota </it>accessions revealed a high level of polymorphism for these selected loci, with an average of 19 alleles/locus and 0.84 expected heterozygosity.</p> <p>Conclusions</p> <p>The addition of 55 SSRs to the carrot map, together with marker characterizations in six other mapping populations, will facilitate future comparative mapping studies and integration of carrot maps. The markers developed herein will be a valuable resource for assisting breeding, genetic, diversity, and genomic studies of carrot and other Apiaceae.</p