115 research outputs found
Characterization of materials used in the manufacture of ceramic tile with incorporation of ornamental rock waste
The production of ceramic tiles, such as tiles, has a great environmental impact, either in
the extraction of natural raw materials or gas emissions in the burning stages. The use of industrial
solid waste in ceramic materials can contribute to the reduction of these impacts, according to the
characteristics of solid waste and its interaction with ceramic materials in the processing steps. Thus,
this study aimed to characterize the materials needed to make a ceramic tile with incorporation of
ornamental rock waste (ORW), thus evaluating its main characteristics regarding the feasibility of this
incorporation. The physical characterization of the clays used in the production of ceramic artifacts
was performed, and for the waste the mineralogical analyzes were performed, through x-ray diffraction
(XRD), microstructure analysis from confocal optical microscopy, after sintering the prototypes and
chemical analysis by X-ray spectroscopy (EDX). Soon after the raw materials went through the step
of conformation and preparation of the prismatic specimens by the process of extrusion of the ceramic
mass, with an incorporation of the ORW in 0% and 15% of the ceramic mass, for its subsequent The
prototypes were sintered at three different temperatures (850 °C, 950 °C and 1,050 °C). The specimens
were submitted to technological tests of mechanical resistance, water absorption, firing shrinkage and
porosity to evaluate the incorporation viability. The results indicated the presence of quartz particles
in all raw materials, and also that the clays of the study region are predominantly kaolinitic. The
presence of these materials in the ceramic masses directly influences the micrographs, because they
result in the formation of liquid phase, inert particles that can turn the site into a stress concentration
point and when incorporated in the ORW the specimens met the technical specifications of the
Brazilian standard for application on ceramic tiles. The results found in the technological tests carried
out, that the incorporation of 15% of ornamental rock waste in both clays did not affect the tile
properties, indicating the feasibility of incorporating this waste in civil construction, minimizing the
impacts generated
Electromagnetic properties of 21O for benchmarking nuclear Hamiltonians
The structure of exotic nuclei provides valuable tests for state-of-the-art
nuclear theory. In particular electromagnetic transition rates are more
sensitive to aspects of nuclear forces and many-body physics than excitation
energies alone. We report the first lifetime measurement of excited states in
O, finding
\,ps. This
result together with the deduced level scheme and branching ratio of several
-ray decays are compared to both phenomenological shell-model and ab
initio calculations based on two- and three-nucleon forces derived from chiral
effective field theory. We find that the electric quadrupole reduced transition
probability of $\rm B(E2;1/2^+ \rightarrow 5/2^+_{g.s.}) = 0.71^{+0.07\
+0.02}_{-0.06\ -0.06}^2^41/2^+$
state, is smaller than the phenomenological result where standard effective
charges are employed, suggesting the need for modifications of the latter in
neutron-rich oxygen isotopes. We compare this result to both large-space and
valence-space ab initio calculations, and by using multiple input interactions
we explore the sensitivity of this observable to underlying details of nuclear
forces.Comment: 23 pages, 3 figure
AWAKE, the advanced proton driven plasma wakefield acceleration experiment at CERN
The Advanced Proton Driven Plasma Wakefield Acceleration Experiment (AWAKE) aims at studying plasma wakefield generation and electron acceleration driven by proton bunches. It is a proof-of-principle R&D experiment at CERN and the world׳s first proton driven plasma wakefield acceleration experiment. The AWAKE experiment will be installed in the former CNGS facility and uses the 400 GeV/c proton beam bunches from the SPS. The first experiments will focus on the self-modulation instability of the long (rms ~12 cm) proton bunch in the plasma. These experiments are planned for the end of 2016. Later, in 2017/2018, low energy (~15 MeV) electrons will be externally injected into the sample wakefields and be accelerated beyond 1 GeV. The main goals of the experiment will be summarized. A summary of the AWAKE design and construction status will be presented
Antibody Complementarity-Determining Regions (CDRs) Can Display Differential Antimicrobial, Antiviral and Antitumor Activities
Background: Complementarity-determining regions (CDRs) are immunoglobulin (Ig) hypervariable domains that determine specific antibody (Ab) binding. We have shown that synthetic CDR-related peptides and many decapeptides spanning the variable region of a recombinant yeast killer toxin-like antiidiotypic Ab are candidacidal in vitro. An alanine-substituted decapeptide from the variable region of this Ab displayed increased cytotoxicity in vitro and/or therapeutic effects in vivo against various bacteria, fungi, protozoa and viruses. the possibility that isolated CDRs, represented by short synthetic peptides, may display antimicrobial, antiviral and antitumor activities irrespective of Ab specificity for a given antigen is addressed here.Methodology/Principal Findings: CDR-based synthetic peptides of murine and human monoclonal Abs directed to: a) a protein epitope of Candida albicans cell wall stress mannoprotein; b) a synthetic peptide containing well-characterized B-cell and T-cell epitopes; c) a carbohydrate blood group A substance, showed differential inhibitory activities in vitro, ex vivo and/or in vivo against C. albicans, HIV-1 and B16F10-Nex2 melanoma cells, conceivably involving different mechanisms of action. Antitumor activities involved peptide-induced caspase-dependent apoptosis. Engineered peptides, obtained by alanine substitution of Ig CDR sequences, and used as surrogates of natural point mutations, showed further differential increased/unaltered/decreased antimicrobial, antiviral and/or antitumor activities. the inhibitory effects observed were largely independent of the specificity of the native Ab and involved chiefly germline encoded CDR1 and CDR2 of light and heavy chains.Conclusions/Significance: the high frequency of bioactive peptides based on CDRs suggests that Ig molecules are sources of an unlimited number of sequences potentially active against infectious agents and tumor cells. the easy production and low cost of small sized synthetic peptides representing Ig CDRs and the possibility of peptide engineering and chemical optimization associated to new delivery mechanisms are expected to give rise to a new generation of therapeutic agents.Department of Education, Universities and Research, Basque GovermentFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Istituto Superiore di Sanita, National Research Project on A.I.D.S.Cariparma Banking FoundationBrazilian National Research CouncilUniv Parma, Sez Microbiol, Dipartimento Patol, I-43100 Parma, ItalyUniv Basque Country, Fac Med Odontol, Dept Inmunol, Microbiol Parasitol, Bilbao, SpainUniv Basque Country, Dept Enfermeria I, Bilbao, SpainUniv Milan, Dipartimento Sci Cliniche L Sacco, Sez Malattie Infettive Immunopatol, Milan, ItalyUniv Studi Parma, Dipartimento Clin Med, Nefrol Sci Prev, Parma, ItalyUniversidade Federal de São Paulo, Departamento Microbiol, Imunol Parasitol, Unidade Oncol Expt, São Paulo, BrazilUniversidade Federal de São Paulo, Dept Biofis, São Paulo, BrazilUniversidade Federal de São Paulo, Departamento Microbiol, Imunol Parasitol, Unidade Oncol Expt, São Paulo, BrazilUniversidade Federal de São Paulo, Dept Biofis, São Paulo, BrazilDepartment of Education, Universities and Research, Basque Goverment: IT-264-07FAPESP: 06/50634-2Istituto Superiore di Sanita, National Research Project on A.I.D.S.: 50G.30Istituto Superiore di Sanita, National Research Project on A.I.D.S.: 40D.14Cariparma Banking Foundation: 2004.0190Brazilian National Research Council: research fellowshipWeb of Scienc
Serum Stabilities of Short Tryptophan- and Arginine-Rich Antimicrobial Peptide Analogs
Several short antimicrobial peptides that are rich in tryptophan and arginine residues were designed with a series of simple modifications such as end capping and cyclization. The two sets of hexapeptides are based on the Trp- and Arg-rich primary sequences from the "antimicrobial centre" of bovine lactoferricin as well as an antimicrobial sequence obtained through the screening of a hexapeptide combinatorial library.HPLC, mass spectrometry and antimicrobial assays were carried out to explore the consequences of the modifications on the serum stability and microbicidal activity of the peptides. The results show that C-terminal amidation increases the antimicrobial activity but that it makes little difference to its proteolytic degradation in human serum. On the other hand, N-terminal acetylation decreases the peptide activities but significantly increases their protease resistance. Peptide cyclization of the hexameric peptides was found to be highly effective for both serum stability and antimicrobial activity. However the two cyclization strategies employed have different effects, with disulfide cyclization resulting in more active peptides while backbone cyclization results in more proteolytically stable peptides. However, the benefit of backbone cyclization did not extend to longer 11-mer peptides derived from the same region of lactoferricin. Mass spectrometry data support the serum stability assay results and allowed us to determine preferred proteolysis sites in the peptides. Furthermore, isothermal titration calorimetry experiments showed that the peptides all had weak interactions with albumin, the most abundant protein in human serum.Taken together, the results provide insight into the behavior of the peptides in human serum and will therefore aid in advancing antimicrobial peptide design towards systemic applications
Inhibition of Enterovirus 71 (EV-71) Infections by a Novel Antiviral Peptide Derived from EV-71 Capsid Protein VP1
Enterovirus 71 (EV-71) is the main causative agent of hand, foot and mouth disease (HFMD). In recent years, EV-71 infections were reported to cause high fatalities and severe neurological complications in Asia. Currently, no effective antiviral or vaccine is available to treat or prevent EV-71 infection. In this study, we have discovered a synthetic peptide which could be developed as a potential antiviral for inhibition of EV-71. Ninety five synthetic peptides (15-mers) overlapping the entire EV-71 capsid protein, VP1, were chemically synthesized and tested for antiviral properties against EV-71 in human Rhabdomyosarcoma (RD) cells. One peptide, SP40, was found to significantly reduce cytopathic effects of all representative EV-71 strains from genotypes A, B and C tested, with IC50 values ranging from 6–9.3 µM in RD cells. The in vitro inhibitory effect of SP40 exhibited a dose dependent concentration corresponding to a decrease in infectious viral particles, total viral RNA and the levels of VP1 protein. The antiviral activity of SP40 peptide was not restricted to a specific cell line as inhibition of EV-71 was observed in RD, HeLa, HT-29 and Vero cells. Besides inhibition of EV-71, it also had antiviral activities against CV-A16 and poliovirus type 1 in cell culture. Mechanism of action studies suggested that the SP40 peptide was not virucidal but was able to block viral attachment to the RD cells. Substitutions of arginine and lysine residues with alanine in the SP40 peptide at positions R3A, R4A, K5A and R13A were found to significantly decrease antiviral activities, implying the importance of positively charged amino acids for the antiviral activities. The data demonstrated the potential and feasibility of SP40 as a broad spectrum antiviral agent against EV-71
Neutrino-driven wind simulations and nucleosynthesis of heavy elements
Neutrino-driven winds, which follow core-collapse supernova explosions,
present a fascinating nuclear astrophysics problem that requires understanding
advanced astrophysics simulations, the properties of matter and neutrino
interactions under extreme conditions, the structure and reactions of exotic
nuclei, and comparisons against forefront astronomical observations. The
neutrino-driven wind has attracted vast attention over the last 20 years as it
was suggested to be a candidate for the astrophysics site where half of the
heavy elements are produced via the r-process. In this review, we summarize our
present understanding of neutrino-driven winds from the dynamical and
nucleosynthesis perspectives. Rapid progress has been made during recent years
in understanding the wind with improved simulations and better micro physics.
The current status of the fields is that hydrodynamical simulations do not
reach the extreme conditions necessary for the r-process and the proton or
neutron richness of the wind remains to be investigated in more detail.
However, nucleosynthesis studies and observations point already to
neutrino-driven winds to explain the origin of lighter heavy elements, such as
Sr, Y, Zr.Comment: Submitted to: J. Phys. G: Nucl. Phy
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