Evolution and dynamics of tropical river plumes in the Great Barrier Reef: an integrated remote sensing and in situ study

The short-lived but intense discharge of freshwater from tropical rivers into the Great Barrier Reef (GBR) Lagoon and the associated salinity reductions are a critical consideration in marine research and management of the ecologically sensitive GBR World Heritage Area. Salinity provides a unique tracer that gives clues to the origin of river-borne contaminants and allows the influences of storm-induced resuspension and river discharge on turbidity to be clearly distinguished. We describe a field investigation of the evolution and dynamics of the Herbert River plume in the central GBR. Its primary goals were to use an airborne salinity mapper and in situ instruments to study the three-dimensional structure and evolution of the plume and to lay a foundation for numerical modeling studies of its dynamics. The aircraft surveys provided a rapid assessment of the plumes spatial extent, while the in situ data revealed details of its subsurface structure. The Herbert River plume was produced by heavy rainfall associated with tropical storms during the La Nina-dominated 1999/2000 monsoon season. In the near field, the surface expression of the plume boundaries was indicated by sharp color and salinity fronts that were clearly visible from the air and sea surface. In the far field and middle Lagoon, the plume was more dispersed and ultimately merged with the larger-scale salinity gradients and with the remnant plume of the more distant, and larger, Burdekin River. The plume location and structure evolved in response to changing river flows, tidal and subtidal circulation, and wind. Using Garvine’s Kelvin number-based scheme, the plume was classified as intermediate in dynamical character and thus is subject to a variety of forcings. The plume evolved in response to changes in the relative intensity of tidal currents and low-frequency circulation due to wind and western boundary current forcing. It also displayed a characteristic ‘‘hook-shaped’’ structure, which has been identified previously in numerical plume model studies. This structure appeared in the presence of accelerating along-shelf current flow and horizontal shear and it indicates that the plume circulation had a strongly three-dimensional character. The approach demonstrates the efficacy of combining airborne and in situ methods to observe rapidly evolving coastal salinity structure and dynamics and sets the stage for future satellite-borne studies of larger-scale features showing contrasting salinity distributions

Evolution and Dynamics of Tropical River Plumes in the Great Barrier Reef: An Integrated Remote Sensing and In Situ Study

[1] The short-lived but intense discharge of freshwater from tropical rivers into the Great Barrier Reef (GBR) Lagoon and the associated salinity reductions are a critical consideration in marine research and management of the ecologically sensitive GBR World Heritage Area. Salinity provides a unique tracer that gives clues to the origin of river-borne contaminants and allows the influences of storm-induced resuspension and river discharge on turbidity to be clearly distinguished. We describe a field investigation of the evolution and dynamics of the Herbert River plume in the central GBR. Its primary goals were to use an airborne salinity mapper and in situ instruments to study the three-dimensional structure and evolution of the plume and to lay a foundation for numerical modeling studies of its dynamics. The aircraft surveys provided a rapid assessment of the plumes spatial extent, while the in situ data revealed details of its subsurface structure. The Herbert River plume was produced by heavy rainfall associated with tropical storms during the La Nina-dominated 1999/2000 monsoon season. In the near field, the surface expression of the plume boundaries was indicated by sharp color and salinity fronts that were clearly visible from the air and sea surface. In the far field and middle Lagoon, the plume was more dispersed and ultimately merged with the larger-scale salinity gradients and with the remnant plume of the more distant, and larger, Burdekin River. The plume location and structure evolved in response to changing river flows, tidal and subtidal circulation, and wind. Using Garvine\u27s Kelvin number-based scheme, the plume was classified as intermediate in dynamical character and thus is subject to a variety of forcings. The plume evolved in response to changes in the relative intensity of tidal currents and low-frequency circulation due to wind and western boundary current forcing. It also displayed a characteristic hook-shaped\u27\u27 structure, which has been identified previously in numerical plume model studies. This structure appeared in the presence of accelerating along-shelf current flow and horizontal shear and it indicates that the plume circulation had a strongly three-dimensional character. The approach demonstrates the efficacy of combining airborne and in situ methods to observe rapidly evolving coastal salinity structure and dynamics and sets the stage for future satellite-borne studies of larger-scale features showing contrasting salinity distributions

A low frequency persistent reservoir of a genomic island in a pathogen population ensures island survival and improves pathogen fitness in a susceptible host

The co-evolution of bacterial plant pathogens and their hosts is a complex and dynamic process. Host resistance imposes stress on invading pathogens that can lead to changes in the bacterial genome enabling the pathogen to escape host resistance. We have observed this phenomenon with the plant pathogen Pseudomonas syringae pv. phaseolicola where isolates that have lost the genomic island PPHGI-1 carrying the effector gene avrPphB from its chromosome are infective against previously resistant plant hosts. However, we have never observed island extinction from the pathogen population within a host suggesting the island is maintained. Here we present a mathematical model which predicts different possible fates for the island in the population; one outcome indicated that PPHGI-1 would be maintained at low frequency in the population long term if it confers a fitness benefit. We empirically tested this prediction and determined that PPHGI-1 frequency in the bacterial population drops to a low but consistently detectable level during host resistance. Once PPHGI-1-carrying cells encounter a susceptible host, they rapidly increase in the population in a negative frequency dependent manner. Importantly, our data show that mobile genetic elements can persist within the bacterial population and increase in frequency under favourable conditions

Vascular Endothelial Growth Factor-Related Pathways in Hemato-Lymphoid Malignancies

Angiogenesis is essential for malignant tumor growth. This has been documented for solid tumors, and there is an emerging evidence suggesting that tumor progression of hematolymphoid malignancies also depends on the induction of new blood vessel formation. The most important proangiogenic agent is vascular endothelial growth factor (VEGF), activating VEGF receptors 1 and 2. The available data on angiogenesis in hemato-lymphoid malignancies, such as acute leukemias, myelodysplastic syndromes, myeloproliferative neoplasms, multiple myeloma, and lymphomas, point towards the significance of autocrine and paracrine VEGF-mediated effects for proliferation and survival of leukemia/lymphoma cells in addition to tumor vascularization. Antiangiogenic strategies have become an important therapeutic modality for solid tumors. Several antiangiogenic agents targeting VEGF-related pathways are also being utilized in clinical trials for the treatment of hemato-lymphoid malignancies, and in some instances these pathways have emerged as promising therapeutic targets. This review summarizes recent advances in the basic understanding of the role of angiogenesis in hemato-lymphoid malignancies and the translation of such basic findings into clinical studies

Rho GTPase function in flies: insights from a developmental and organismal perspective.

Morphogenesis is a key event in the development of a multicellular organism and is reliant on coordinated transcriptional and signal transduction events. To establish the segmented body plan that underlies much of metazoan development, individual cells and groups of cells must respond to exogenous signals with complex movements and shape changes. One class of proteins that plays a pivotal role in the interpretation of extracellular cues into cellular behavior is the Rho family of small GTPases. These molecular switches are essential components of a growing number of signaling pathways, many of which regulate actin cytoskeletal remodeling. Much of our understanding of Rho biology has come from work done in cell culture. More recently, the fruit fly Drosophila melanogaster has emerged as an excellent genetic system for the study of these proteins in a developmental and organismal context. Studies in flies have greatly enhanced our understanding of pathways involving Rho GTPases and their roles in development

Risk-factors for non-adherence to antiretroviral therapy

Cross-sectional study analyzed as case-control to identify risk factors for non-adherence to antiretroviral therapy. We studied 412 out-clinics HIV infected subjects of three public hospitals of Recife, Pernambuco. The objective was to examine the association between non-adherence to the antiretroviral therapy and biological, social-behavior and demographics and economic factors, factors related to the disease and/or treatment, factors related to life habits and depression symptoms. Variables significantly associated with non-adherence to antiretroviral therapy were: time elapsed since HIV diagnosis (p = 0.002), daily dose (p = 0.046), use of alcohol (p = 0.030) and past drug use (p = 0.048), and borderline p-values were found for educational level (p = 0.093) and family monthly income (p = 0.08). In the multivariable analysis, the factors that remained in the final model were family monthly income, time period with HIV infection and use of alcohol. No association was observed between non-adherence to antiretroviral therapy and gender, age, sexual orientation, marital status, educational level and place of residence. Based on our results and the local situation we suggest: assessment of social needs; training of partners and/or families on supporting adherence, creation of "adherence groups" to motivate and to reassure patients on the benefits of treatment; counseling and/or psychotherapy for alcohol drinkers

AD51B in Familial Breast Cancer

Common variation on 14q24.1, close to RAD51B, has been associated with breast cancer: rs999737 and rs2588809 with the risk of female breast cancer and rs1314913 with the risk of male breast cancer. The aim of this study was to investigate the role of RAD51B variants in breast cancer predisposition, particularly in the context of familial breast cancer in Finland. We sequenced the coding region of RAD51B in 168 Finnish breast cancer patients from the Helsinki region for identification of possible recurrent founder mutations. In addition, we studied the known rs999737, rs2588809, and rs1314913 SNPs and RAD51B haplotypes in 44,791 breast cancer cases and 43,583 controls from 40 studies participating in the Breast Cancer Association Consortium (BCAC) that were genotyped on a custom chip (iCOGS). We identified one putatively pathogenic missense mutation c.541C>T among the Finnish cancer patients and subsequently genotyped the mutation in additional breast cancer cases (n = 5259) and population controls (n = 3586) from Finland and Belarus. No significant association with breast cancer risk was seen in the meta-analysis of the Finnish datasets or in the large BCAC dataset. The association with previously identified risk variants rs999737, rs2588809, and rs1314913 was replicated among all breast cancer cases and also among familial cases in the BCAC dataset. The most significant association was observed for the haplotype carrying the risk-alleles of all the three SNPs both among all cases (odds ratio (OR): 1.15, 95% confidence interval (CI): 1.11–1.19, P = 8.88 x 10−16) and among familial cases (OR: 1.24, 95% CI: 1.16–1.32, P = 6.19 x 10−11), compared to the haplotype with the respective protective alleles. Our results suggest that loss-of-function mutations in RAD51B are rare, but common variation at the RAD51B region is significantly associated with familial breast cancer risk