The MMS22L-TONSL Complex Mediates Recovery from Replication Stress and Homologous Recombination

Genome integrity is jeopardized each time DNA replication forks stall or collapse. Here we report the identification of a complex composed of MMS22L (C6ORF167) and TONSL (NFKBIL2) that participates in the recovery from replication stress. MMS22L and TONSL are homologous to yeast Mms22 and plant Tonsoku/Brushy1, respectively. MMS22L-TONSL accumulates at regions of ssDNA associated with distressed replication forks or at processed DNA breaks, and its depletion results in high levels of endogenous DNA double-strand breaks caused by an inability to complete DNA synthesis after replication fork collapse. Moreover, cells depleted of MMS22L are highly sensitive to camptothecin, a topoisomerase I poison that impairs DNA replication progression. Finally, MMS22L and TONSL are necessary for the efficient formation of RAD51 foci after DNA damage, and their depletion impairs homologous recombination. These results indicate that MMS22L and TONSL are genome caretakers that stimulate the recombination-dependent repair of stalled or collapsed replication forks

Effects of customer incivility on frontline employees and the moderating role of supervisor leadership style

Customer incivility toward frontline employees (FLEs) is a widespread phenomenon within tourism and hospitality industries, severely depleting the psychological resources of FLEs and delivered customer service. Drawing on the job demands-resources and conservation of resources frameworks, the current research compares the effects of the two most common forms of customer incivility on FLEs’ psychological responses and behavioral intentions (study 1). Moreover, this work explores the degree to which supervisor leadership style can mitigate the depleting effects of these two forms of customer incivility on FLEs (study 2). Findings demonstrate that FLEs’ responses to customer incivility episodes remain contingent upon supervisor’s leadership style and acknowledge that an empowering (vs. laissez-faire) leadership style can better mitigate the depleting effects of both customer incivility forms on FLEs’ role stress, rumination, retaliation and withdrawal intentions. The implications of these findings for tourism and hospitality theory and practicing managers are discussed

Alzheimer's disease genes in zebrafish (Danio rerio) / Casper Groth.

"August 2003"Bibliography: leaves 166-195.vii, 195 leaves : ill. (col.); plates (col.) ; 30 cm.It was felt that investigations of the mechanisms controlling developmental processes in different embryonic tissues may be a relevant approach in order to gain insight into the normal biological functions of Alzheimer's disease related genes, and, ultimately the cause of neurodegeneration as well as other pathological conditions in the adult. Therefore, it was decided to dissect the functions of Alzheimer's disease genes during embryo development using the zebra fish (Danio rerio) as a model organism.Thesis (Ph.D.)--University of Adelaide, School of Molecular and Biomedial Science, Discipline of Genetics, 200